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Andrea Cavallini
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office(s):
MXG321
phone(s): [+41 21 69] 36878
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BIOGRAPHY
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Andrea Cavallini got a bachelor degree in Pharmaceutical Biotechnology at the University of Ferrara and a master degree in Molecular Biotechnology at the University of Bologna.
Currently, he is a PhD student in Microsystems and Microelectronics at the Ecole Politechnique Federale de Lausanne (EFPL), under the direction of professors Sandro Carrara and Giovanni de Micheli.
His main research interest focuses on the development of point-of-care and implantable cytochrome P450-based nanochip arrays for diagnostic applications. He also did research in the characterization and biofunctionalization of thin films for the realization of label free DNA microarrays based on capacitance detection.
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PhD THESIS TOPIC
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PROTEIN NANOCHIP ARRAYS FOR DIAGNOSTIC APPLICATIONS
The objective of this thesis is to develop a smart nanotechnology based biosensor for point-of-care drug metabolism analysis, which significantly improves the quality and reliability of drug concentration measurements, providing at the same time a reduction in the cost and time of analysis. In particular, the system will quantify concentrations of most drugs and those of endogenous substances that are substrates of cytochrome P450 enzymes.
P450’s have a key role in the metabolism of drugs and can represent a potential target for the development of platforms for personalized therapy.
Enzyme Biosensors based on a variety P450 proteins have already been developed, however the detection of drug mixtures with enzyme biosensors rapresent an inovation. Multiple drugs detection with cytochromes is a challenging task because different P450 isoforms are not selective for a single compound, and are subjected to several atypical kinetic features that will affect the protein efficiency according to the concentration and the type of the drugs present in the sample analyzed.
A parallel topic considered in this project will be the development of a biocompatible envelope for the sensor, which will allow its implantation in living subjects.
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MAIN PUBLICATIONS
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New insights for using self-assembly materials to improve the detection stability in label-free DNA-chip and immuno-sensors, Carrara, S., Benini, L., Bhalla, V., Stagni, C., Ferretti, A., Cavallini, A., Riccò, B., Samorì, B., 2009, Biosensors and Bioelectronics 24(12), 3425-3429
Circuits Design and Nano-Structured Electrodes for Drugs Monitoring in Personalized Therapy, Carrara, S., Cavallini, A., De Micheli, G., Olivo, J., Benini, L., Shumyantseva, V., Archakov, A., in proceedings of IEEE BioCAS 2008, Biomedical Circuits and Systems Conference, Baltimore, 20-22 November 2008, pp. 325-328, ISBN: 978-1-4244-2879-3/08
Dynamical Spot Queries to Improve Specificity in P450s based Multi-Drugs Monitoring, Carrara, S., Cavallini, A., Garg, A., De Micheli, G., proceedings of the IEEE/ICME International Conference on Complex Medical Engineering, April 9-11, 2009, Tampe, Arizona, U.S.A.
New probe immobilizations by Lipoate-Diethalonamines or Ethylene-Glycol molecules for Capacitance DNA Chip, Carrara, S., Cavallini, A., Leblebici, Y., De Micheli, G., Bhalla, V., Valle, F., Benini, B., Riccò, B., Vikholm-Lundin, I., Munter, T., IEEE Conference Proceedings of IWASI, Trani (Bari), June 2009, 9-14
3D Architecture and Replaceable Layers for Label-Free DNA Biochips, Temiz, Y., Carrara, S., Cavallini, A., Leblebici, Y., De Micheli, G., accepted for publication in IEEE Conference Proceedings of IWASI, Trani (Bari), June 2009, 35-40.
Capacitance DNA Bio-Chips improved by new Probe Immobilization Strategies, Carrara, S., Cavallini, A., Leblebici, Y., De Micheli, G., Bhalla, V., Valle, F., Samori, B., Benini, L., Riccò, B., Vikholm-Lundin, I., Munter, T., Microelectronics Journal 41(11), 711-717.
New Ethylene Glycol-Silanes monolayer for highly-specific DNA detection on Silicon Chips, Sandro Carrara, Andrea Cavallini, Yuki Maruyama, Edoardo Charbon, Giovanni De Micheli, Surface Science 604 (2010), pp. L71-L74
Multiplexing pH and Temperature in a Molecular Biosensor, Sandro Carrara, Michele Daniel Torre, Andrea Cavallini, Daniela De Venuto, Giovanni De Micheli, IEEE International Conference BioCAS 2010, Cyprus November 3-5, 2010, pag. 146-149
P450-Mediated Electrochemical Sensing of Drugs in Human Plasma for Personalized Therapy, Andrea Cavallini, Sandro Carrara, Victor Erokhin, Giovanni De Micheli, IEEE International Conference PRIME 2010, Berlin, 18-21 July 2010, 1-4
Multi-panel drugs detection in human serum for personalized therapy, Sandro Carrara, Andrea Cavallini, Victor Erokhin, Giovanni de Micheli, 2011 Biosensors and Bioelectronics 26(9), 3914-3919
A new Ethylene-Glycol-Silanes monolayer for highly-specific DNA detection onto Silicon Chip, Carrara, S., Cavallini, A., Maruyama, Y., Charbon, E., De Micheli, G., 2010. A new ethylene glycol-silane monolayer for highly-specific DNA detection on Silicon Chips. Surface Science 604(23-24), L71-L74.
Comparison of Three Methods of Biocompatible Multi-walled Carbon Nanotubes Confinement For The Development of Implantable Amperometric ATP Biosensors, Andrea Cavallini, Sandro Carrara, Giovanni de Micheli, Sensor letters - submitted
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AWARDS
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"Best Poster Award" at the 7th Nanoeurope symposium - Rapperswil 2009
"Bronze Leaf Award" at PRIME 2010 conference (Berlin, 19-21 july)
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Doctoral Assistant