Florence Pojer

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Head of Unit

florence.pojer@epfl.ch +41 21 693 19 76 https://www.epfl.ch/research/facilities/ptpsp/

Citizenship: Swiss-French

EPFL SV PTECH PTPSP
AI 2147 (Bâtiment AI)
Station 19
1015 Lausanne

+41 21 693 19 76
Office: AI 2147
EPFL > SV > PTECH > PTPSP

Web site: Web site: https://www.epfl.ch/research/facilities/ptpsp/

+41 21 693 19 76
EPFL > VPA-AVP-DLE > AVP-DLE-EDOC > EDMS-ENS

Web site: Web site: https://go.epfl.ch/edms

+41 21 693 19 76
EPFL > SV > SV-SSV > SSV-ENS

Web site: Web site: https://sv.epfl.ch/education

+41 21 693 19 76
EPFL > P > P-EM > AE

Web site: Web site: https://ae.epfl.ch/

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Administrative data

Fields of expertise

Head of a core facility within the Life Sciences School - PTPSP
With main expertise in Integrative protein Structural Biology (e.g. biophysical techniques, X-ray Crystallography, SPR Cryo-EM,..)
And production/Purification of macromolecules (e.g. antibodies, membrane proteins, growth factors...) expressed in mammalian, insect and bacterial cells

Mission

PTPSP is a hub for Protein Sciences and Integrative Structural Biology; with top-notch instrumentations, close connections to high-end facilities and a dedicated team of experts highly enthusiastic to help your project move forward. On a daily basis, the team advises, supports and trains scientists in production, purification and biophysical/structural characterisations of macromolecules, such as enzymes, antibodies or any kind of proteins you are interested in.

Professional course

Head of Protein Production and Structure Core Facility

School of Life Sciences

EPFL

2011-now

Staff scientist

Chair of Microbial Pathogenesis, Prof. Stewart Cole

EPFL

2007-2018

Post-doc

Structural Biology lab, Prof. Joseph Noel

The Salk Institute (La Jolla, USA)

2004-2007

Education

PhD thesis

Antibiotic research

Tuebingen (Germany)

1999-2003

Pharm D

Pharmacy School

Besancon (France)

1992-1998

Publications

Infoscience publications

Antibody-peptide conjugates deliver covalent inhibitors blocking oncogenic cathepsins

A. S. Petruzzella; M. Bruand; A. Santamaria-Martinez; N. Katanayeva; L. Reymond et al.

Nature Chemical Biology. 2024-05-29. DOI : 10.1038/s41589-024-01627-z.

Florence Pojer

Head of Unit

Fields of expertise

Mission

Professional course

Education

Publications

Infoscience publications

Antibody-peptide conjugates deliver covalent inhibitors blocking oncogenic cathepsins

Manganese Transfer Hydrogenases Based on the Biotin-Streptavidin Technology

Machine learning predictions of MHC-II specificities reveal alternative binding mode of class II epitopes

High-affinity peptides developed against calprotectin and their application as synthetic ligands in diagnostic assays

Uncovering and engineering the mechanical properties of the adhesion GPCR ADGRG1 GAIN domain

Cryo-EM structures and binding of mouse and human ACE2 to SARS-CoV-2 variants of concern indicate that mutations enabling immune escape could expand host range

Broadly potent anti-SARS-CoV-2 antibody shares 93% of epitope with ACE2 and provides full protection in monkeys

Structure-based optimization of type III indoleamine 2,3-dioxygenase 1 (IDO1) inhibitors

Patient-derived monoclonal antibody neutralizes SARS-CoV-2 Omicron variants and confers full protection in monkeys

A highly potent antibody effective against SARS-CoV-2 variants of concern

A high-throughput cell- and virus-free assay shows reduced neutralization of SARS-CoV-2 variants by COVID-19 convalescent plasma

A Nanoscaffolded Spike-RBD Vaccine Provides Protection against SARS-CoV-2 with Minimal Anti-Scaffold Response

Palmitoylated acyl protein thioesterase APT2 deforms membranes to extract substrate acyl chains

Azole-Based Indoleamine 2,3-Dioxygenase 1 (IDO1) Inhibitors

Changes in SARS-CoV-2 Spike versus Nucleoprotein Antibody Responses Impact the Estimates of Infections in Population-Based Seroprevalence Studies

Structural investigation of ACE2 dependent disassembly of the trimeric SARS-CoV-2 Spike glycoprotein

Selective inhibition of STAT3 signaling using monobodies targeting the coiled-coil and N-terminal domains

High resolution CryoEM structure of the ring-shaped virulence factor EspB from Mycobacterium tuberculosis

De novo development of proteolytically resistant therapeutic peptides for oral administration

Btk SH2-kinase interface is critical for allosteric kinase activation and its targeting inhibits B-cell neoplasms

Inhibition Mechanisms of Indoleamine 2,3-Dioxygenase 1 (IDO1)

Structural and functional dissection of the DH and PH domains of oncogenic Bcr-Abl tyrosine kinase

Selective Targeting of SH2 Domain–Phosphotyrosine Interactions of Src Family Tyrosine Kinases with Monobodies

A rheostat mechanism governs the bifurcation of carbon flux in mycobacteria

Discovery of benzothiazoles as antimycobacterial agents: Synthesis, structure-activity relationships and binding studies with Mycobacterium tuberculosis decaprenylphosphoryl-beta-D-ribose 2 '-oxidase

Lansoprazole is an antituberculous prodrug targeting cytochrome bc1

Structure of EspB, a secreted substrate of the ESX-1 secretion system of Mycobacterium tuberculosis

2-Carboxyquinoxalines Kill Mycobacterium tuberculosis through Noncovalent Inhibition of DprE1

Internalization and vacuolar targeting of the brassinosteroid hormone receptor BRI1 are regulated by ubiquitination

The Crystal Structures of Apo and cAMP-Bound GlxR from Corynebacterium glutamicum Reveal Structural and Dynamic Changes upon cAMP Binding in CRP/FNR Family Transcription Factors

Dithiol amino acids can structurally shape and enhance the ligand-binding properties of polypeptides

The cysteine desulfurase IscS of Mycobacterium tuberculosis is involved in iron sulfur cluster biogenesis and oxidative stress defence

Functional Dissection of Intersubunit Interactions in the EspR Virulence Regulator of Mycobacterium tuberculosis

Towards a new combination therapy for tuberculosis with next generation benzothiazinones

Pyridomycin bridges the NADH- and substratebinding pockets of the enoyl reductase InhA

Peptide ligands stabilized by small molecules

Structure and Function of Essential Components of Contractile Injection Systems

Phenotypic Profiling of Mycobacterium tuberculosis EspA Point Mutants Reveals that Blockage of ESAT-6 and CFP-10 Secretion In Vitro Does Not Always Correlate with Attenuation of Virulence

Mycobacterium tuberculosis EspB binds phospholipids and mediates EsxA-independent virulence

Tetrahydrobiopterin Biosynthesis as an Off-Target of Sulfa Drugs

Structural Basis for Benzothiazinone-Mediated Killing of Mycobacterium tuberculosis

Towards a new tuberculosis drug: pyridomycin - nature's isoniazid

Characterization of Molecular Determinants of the Conformational Stability of Macrophage Migration Inhibitory Factor: Leucine 46 Hydrophobic Pocket

Virulence Regulator EspR of Mycobacterium tuberculosis Is a Nucleoid-Associated Protein

Directed evolution of the suicide protein O⁶-alkylguanine-DNA alkyltransferase for increased reactivity results in an alkylated protein with exceptional stability

Structure and function of the transketolase from Mycobacterium tuberculosis and comparison with the human enzyme

Benzothiazinones Are Suicide Inhibitors of Mycobacterial Decaprenylphosphoryl-β-

EspD Is Critical for the Virulence-Mediating ESX-1 Secretion System in Mycobacterium tuberculosis

Atypical DNA recognition mechanism used by the EspR virulence regulator of Mycobacterium tuberculosis

Towards anti-virulence drugs targeting ESX-1 mediated pathogenesis of Mycobacterium tuberculosis

Sigma Factor F does not Prevent Rifampin Inhibition of RNA Polymerase or Cause Rifampin Tolerance in Mycobacterium tuberculosis

A finite element model of the LHC dipole cold mass with hysteretic, non-linear behavior and single turn description

Teaching & PhD

Teaching

Outreach

Courses

Concept to early-stage drug and medTech products

Integrative structural biology for Life sciences

Recombinant protein expression in animal cells for appli-cations in medicine and structural biology

Methods: from disease models to therapy

Methods: omics in biomedical research

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