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Suliana Manley
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Assistant professor (July, 2009)
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office(s):
BSP515
phone(s): [+41 21 69] 30632,35574
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MISSION
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Cellular responses to external signals begin at the plasma membrane, where the dynamic assembly of receptors or ion channels can regulate cellular activity. Membrane-enveloped viruses, including the human immunodeficiency virus (HIV) also assemble at the plasma membrane, exploiting mechanisms evolved for cellular trafficking. Despite the importance of these processes, our physical paradigm for how proteins form mesoscale assemblies is far from complete.
This is in part a consequence of technical limitations. While the organization and dynamics of membrane proteins are heterogeneous, commonly used fluorescence-based measurements lack information at the molecular scale. In contrast, single molecule measurements limited to looking at only a few molecules in a given cell lack ensemble information. Thus, the study of protein assembly has been limited by a lack of spatially resolved, dynamic information on ensembles of molecules. To overcome these obstacles, we use super-resolution fluorescence imaging techniques combined with live cell imaging and single molecule tracking to determine how the dynamics of protein assembly are coordinated.
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BIOGRAPHY
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From 06/2009 Tenure-track assistant professor, EPFL, Lausanne, Switzerland
2006-2009 Post-Doctoral fellow, National Institutes of Health, Bethesda, MD, USA
2004-2006 Post-Doctoral fellow, Massachusetts Institute of Technology, Cambridge, MA, USA
1999-2004 PhD (Physics) Awarded 06/2004, Harvard University, Cambridge, MA, USA
1993-1997 Bachelors (Cum Laude) Physics & Mathematics, Rice University, Houston, TX, USA
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MAIN PUBLICATIONS
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14. F.V. Subach, G.H. Patterson, S. Manley, J.M. Gillette, J. Lippincott-Schwartz and V.V. Verkhusha “Photoactivatable mCherry for high-resolution two-color fluorescence microscopy,” In press (Nature Methods)
13. G. Shtengel, J.A. Galbraith, C.G. Galbraith, J. Lippincott-Schwartz, J.M. Gillette, S. Manley, R. Sougrat, C.M. Waterman, P. Kanchanawong, M. Davidson, R. Fetter and H.F. Hess “Interferometric Fluorescent Super-Resolution Microscopy Resolves 3D Cellular Ultrastructure,” In press (Proc. Natl. Acad. Sci. USA)
12. S. Manley, J. M. Gillette, H. Shroff, H. Hess, E. Betzig and J. Lippincott-Schwartz “High-density mapping of single-molecule trajectories with photoactivated localization microscopy,” Nature Methods 5(2):155-157 (2008) Featured in Nature Methods News and Views, Faculty of 1000: ‘Must Read’
11. S. Manley, M.R. Horton, S. Leszczynski and A.P. Gast, “Sorting of protein crystals on phase-separating membranes,” Biophysical Journal 95:2301-2307 (2008)
10. A.E. Bailey, W.C.K. Poon, R.J. Christianson, A.B. Schofield, U. Gasser, V. Prasad, S. Manley, P.N. Segre, L. Cipelletti, W.V. Meyer, M.P. Doherty, S. Sankaran, A.L. Jankovsky, W.L. Shiley, J.P. Bowen, J.C. Eggers, C. Kurta, T. Lorik, P.N. Pusey, and D.A. Weitz, “Spinodal decomposition in a model colloid-polymer mixture in microgravity,” Physical Review Letters 99:205701 (2007)
9. M.R. Horton, S. Manley, S. Arevalo, A.E. Lobkovsky and A.P. Gast, “Crystalline protein domains and lipid bilayer vesicle shape transformations,” Journal of Physical Chemistry B 111:880-885 (2007)
8. G. Popescu, T. Ikeda, K. Goda, C.A. Best, M. Laposata, S. Manley, R.R. Dasari, K. Badizadegan and M. Feld, “Optical Measurement of Red Blood Cell Membrane Tension,” Physical Review Letters 97:218101 (2006)
7. S. Manley, H.M. Wyss, K. Miyazaki, J. Conrad, V. Trappe, L.J. Kaufman, D.R. Reichman and D.A. Weitz, “Glasslike Arrest in Spinodal Decomposition as a Route to Gelation,” Physical Review Letters 95:238302 (2005) Featured in Research Highlights, Nature 441:670-671 ( 2006)
6. S. Manley, B. Davidovitch, N.R. Davies, L. Cipelletti, A.E. Bailey, R.J. Christianson, U. Gasser, V. Prasad, P.N. Segre, M.P. Doherty, S. Sankaran, A.L. Jankovsky, W.L. Shiley, J. Bowen, J. Eggers, C. Kurta, T. Lorik and D.A. Weitz, “Time-Dependent Strength of Colloidal Gels,” Physical Review Letters 95:048302 (2005)
5. S. Manley, J.M. Skotheim, L. Mahadevan and D.A. Weitz, “Gravitational Collapse of Colloidal Gels,” Physical Review Letters 94:218302 (2005)
4. S. Manley, L. Cipelletti, V. Trappe, A.E. Bailey, R.J. Christianson, U. Gasser, V. Prasad, P.N. Segre, M.P. Doherty, S. Sankaran, A.L. Jankovsky, W.L. Shiley, J. Bowen, J. Eggers, C. Kurta, T. Lorik and D. A. Weitz, “Limits to Gelation in Colloidal Aggregation,” Physical Review Letters 93:108302 (2004)
3. Luca Cipelletti, Laurence Ramos, S. Manley, E. Pitard, D.A. Weitz, Eugene E. Pashkovski and Marie Johansson, “Universal non-diffusive slow dynamics in aging soft matter,” Faraday Discussions 123:237-251 (2003)
2. Shang-You Tee, P.J. Mucha, Luca Cipelletti, S. Manley, M.P. Brenner, P.N. Segre and D.A. Weitz, “Nonuniversal Velocity Fluctuations of Sedimenting Particles,” Physical Review Letters 89:054501 (2002)
1. Luca Cipelletti, S. Manley, R.C. Ball and D.A. Weitz, “Universal Aging Features in Restructuring of Fractal Colloidal Gels,” Physical Review Letters 84:2275 (2000)
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POSITIONS AVAILABLE
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PhD and postdoctoral positions available. Email for more information.
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| Phd programs |
- Molecular Biology of Cancer and Infection
- Physics (edoc)
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| Phd Students |
Gunzenhäuser Julia
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