Kristin Stefanie Grussmayer

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Academic Profile
I am a biophysicist using advanced microscopy to address specific problems in (neuro)biology, (bio)chemistry and material sciences using molecular scale readout. In my research, I collaborate with biologists, chemists, engineers and physicists to identify new strategies to complement traditional measurement tools. My multidisciplinary approach is based on a general physics education with specialization in biophysics. In my doctorate I worked on quantification of fluorescent molecules by photon statistics (antibunching) in the single molecule spectroscopy group of Prof. D.-P. Herten at Heidelberg University. I could establish the method as a robust tool that is compatible with a wide range of dyes. In first applications, I investigated the label number distributions of commonly used biological fluorescent markers and could reveal strikingly different fluorescence quenching behaviours in single conjugated polymer chains. At EPFL, I am extending my quantitative microscopy expertise to 3D fluorescence super-resolution and phase imaging. My current focus lies on developing and adapting new microscopy techniques for application in neurobiology. Specifically, I am interested in the direct monitoring of alpha-synuclein aggregates in Parkinson compromised neurons and the resulting overall pathogenic response of the cell. I am using a multi-plane microscope for structural and fast functional imaging to (better) understand the underlying molecular mechanism of the disease.

BM 5134 (Bâtiment BM)
Station 17
CH-1015 Lausanne

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Administrative data

Fields of expertise

Optics/Physical Chemistry

Super-resolution Fluorescence Microscopy, ensemble and single-molecule fluorescence spectroscopy, AlEX-FRET, fluorophore/protein quantification (photon antibunching, photobleaching step analysis, SOFI, ...), single-molecule kinetics


protein preparation and purification, surface immobilization, fluorescence labeling, DNA biosensors and transcription factors, PAGE and EMSA, cell culture, immunofluorescence

Computational skills

Mac and PC systems, Matlab, Latex, Monte Carlo Simulation