Kristin Stefanie Grussmayer
Academic ProfileI am a biophysicist using advanced microscopy to address specific problems in (neuro)biology, (bio)chemistry and material sciences using molecular scale readout. In my research, I collaborate with biologists, chemists, engineers and physicists to identify new strategies to complement traditional measurement tools. My multidisciplinary approach is based on a general physics education with specialization in biophysics. In my doctorate I worked on quantification of fluorescent molecules by photon statistics (antibunching) in the single molecule spectroscopy group of Prof. D.-P. Herten at Heidelberg University. I could establish the method as a robust tool that is compatible with a wide range of dyes. In first applications, I investigated the label number distributions of commonly used biological fluorescent markers and could reveal strikingly different fluorescence quenching behaviours in single conjugated polymer chains. At EPFL, I am extending my quantitative microscopy expertise to 3D fluorescence super-resolution and phase imaging. My current focus lies on developing and adapting new microscopy techniques for application in neurobiology. Specifically, I am interested in the direct monitoring of alpha-synuclein aggregates in Parkinson compromised neurons and the resulting overall pathogenic response of the cell. I am using a multi-plane microscope for structural and fast functional imaging to (better) understand the underlying molecular mechanism of the disease.
Fields of expertise
Super-resolution Fluorescence Microscopy, ensemble and single-molecule fluorescence spectroscopy, AlEX-FRET, fluorophore/protein quantification (photon antibunching, photobleaching step analysis, SOFI, ...), single-molecule kinetics
protein preparation and purification, surface immobilization, fluorescence labeling, DNA biosensors and transcription factors, PAGE and EMSA, cell culture, immunofluorescence
Mac and PC systems, Matlab, Latex, Monte Carlo Simulation