Louise Helene Soegaard Jensen
Phone numbers
Office: 021 692 44 22 SEM lab: 021 692 43 53
EPFL ENAC IIE LGB
GEO 3236 (Géopolis UNIL)
Rue de la Mouline 11
CH-1015 Lausanne
+41 21 6924353
+41 21 6924373
Site web: http://lgb.epfl.ch
Unité: LGB
Local: GEO 3236
Local: GEO 3236
+41 21 6924373
Site web: http://lgb.epfl.ch
Unité: LGB
Local: GEO 3236
Local: GEO 3236
Biography
Jan 2018-Present: Scientific collaborator, EPFL
Cryo-SEM and cryo-preparation. Method development, prep- and microscopy service for users.
Jan 2015-Dec 2017: Postdoc, UNIL & EPFL
Planning and customizing a novel cryo-SEM, which at the present moment is among the most advanced cryo-SEM machines in the world.
Writing the request for tender and implementing a full prep lab for cryoSEM.
Cryo-NanoSIMS method development and electron microscopy.
Oct 2013-Dec 2014: Postdoc, DTU Center for Electron Nanoscopy
Electron microscopy in Nanotoxicology. Total activities in DTU: http://bit.ly/2fRbP9v
Jun 2013-Oct 2013: Research assistant, DTU Center for Electron Nanoscopy (CEN)
Setting up a prep lab for biological electron microscopy in Center for Electron Nanoscopy, DTU
Oct 2009-Jun 2013: PhD student, DTU Mechanical Engineering (and affiliated with DTU CEN)
Electron microscopy of the ultrastructure of emulsions. Supervisor: Andy Horsewell.
Apr 2008-Oct 2009: Research assistant, DTU Aqua
Chemical and sensorial analysis and correlation by multivariate statistics in studies of freezing and thawing of fish.
2007. M.Sc. Engineering, Biotechnology & molecular microbiology. Technical University of Denmark.
Thesis work, 1 year, on the function of a gene which is important for DNA repair and certain cancer types: “Characterization of the functional importance of rad52 mutants in spontaneous recombination and DNA double strand break repair”
Awards and Scholarships
Exceptional paper 2017 in Environmental Toxicology & Chemistry.
Awarded the Don Claugher Bursary from the Society of Electron Microscope Technology, 2013.
Research visits and courses
January 2018 Basics of Tokuyasu Techniques, Cellbiology, UMC Utrecht.
November 2014 Zeiss Merlin workshop, Umeå Core Facility Electron Microscopy, Sweden. Critical point drying and energy selective low kV SEM.
May 2014 CEMOVIS training by Helmut Gnaegi, King’s College London, England.
April October-November 2014 Research stays at Centre for Ultrastructural Imaging, King’s College London, England. High pressure freezing, CEMOVIS, freeze drying, STEM EDX, 3View SEM.
January-April 2012, June-August 2012 October 2012 Research stays at Electron Microscopy Facility (EMF), Université de Lausanne, Switzerland. High pressure freezing and freeze-substitution, cryoSEM, TEM.
January 2011 CryoTEM work at Kemicentrum, Lunds Universitet, Sweden.
March 2011 Research stay at Biozentrum, Martin Luther Universität Halle-Wittenberg, Germany. Propane jet freezing and freeze-fracture replicas.
October 2010 EELS-EFTEM meeting, ETH Zürich, Switzerland.
June 2010 High Pressure Freezing & cryoSEM at Electron Microscopy ETH Zürich (EMEZ), Switzerland.
March 2010 Cool snaps, cryo electron microscopy course, Rothamstead research station, England.
January 2010 Winter school, cryo transmission electron tomography. Electron Microscopy ETH Zürich (EMEZ), Switzerland.
Other education
2011 University level teaching, DTU.
2009 Multivariate statistics course, CAMO.
2007-2008 Certified leader coach.
Compétences
Electron microscopy of soft materials. Especially cryo-SEM, low voltage SEM and related prep techniques.
Microscopy:
(cryo-) SEM, VP-SEM, FIBSEM, (cryo-) TEM, STEM, EDX, CLSM, fluorescence microscopy. Method development for CryoNanoSIMS.
Experience with a multitude of sample types such as unicellular organisms, biofilms, invertebrates, polymers, liquid suspensions, nanoparticles, emulsions, building materials, sediments, foodstuff and plants.
Prep:
Experience with all freezing methods for cryo-SEM and –TEM including all brands of high pressure freezers, slam freezing, propane jet freezing, guillotine systems and plunge freezers. Experience with all cold stage systems on the market for cryo-SEM. Ultramicrotomy: standard, serial, cryo and cryo-planing. Freeze substitution, freeze drying, standard chemical fixation etc.
Other:
3D reconstruction of microscopy data from FIB, 3View and array tomography. Multivariate statistics and modelling. Microbiology and molecular biology methods. Sensory and chemical analysis.
Microscopy:
(cryo-) SEM, VP-SEM, FIBSEM, (cryo-) TEM, STEM, EDX, CLSM, fluorescence microscopy. Method development for CryoNanoSIMS.
Experience with a multitude of sample types such as unicellular organisms, biofilms, invertebrates, polymers, liquid suspensions, nanoparticles, emulsions, building materials, sediments, foodstuff and plants.
Prep:
Experience with all freezing methods for cryo-SEM and –TEM including all brands of high pressure freezers, slam freezing, propane jet freezing, guillotine systems and plunge freezers. Experience with all cold stage systems on the market for cryo-SEM. Ultramicrotomy: standard, serial, cryo and cryo-planing. Freeze substitution, freeze drying, standard chemical fixation etc.
Other:
3D reconstruction of microscopy data from FIB, 3View and array tomography. Multivariate statistics and modelling. Microbiology and molecular biology methods. Sensory and chemical analysis.