Matteo Cornaglia

Collaborateur scientifique

Site web: http://lmis2.epfl.ch/
Unité: LMIS2

Unité: PSEFORGE

Mission

We conceive technological solutions for the automated analysis of the nematode Caenorhabditis elegans, one of the most employed model organisms in biomedical research. Specifically, we develop microfluidic platforms for on-chip nematode culture and creation of synchronized C. elegans micro-arrays, long-term multi-dimensional imaging of both developing embryos and worms and systematic phenotyping studies on C. elegans at single-organism resolution. The main fields of application of our technology are developmental biology and research on metabolic and neurodegenerative diseases.

Biography

Matteo Cornaglia was born in Bra, Italy in 1986. He received his B.s. degree, with high honors, in Engineering physics from Politecnico di Torino (Turin, Italy) in 2008. In 2010, he then obtained his M.s. degree, with high honors, in Nanotechnologies for ICT, as a joint study agreement between Politecnico di Torino (Turin, Italy), Institut National Polytechnique de Grenoble (Grenoble, France) and École Polytechnique Féderal de Lausanne (Lausanne, Switzerland). He carried on research projects as intern student at Microla Optoelectronics (Turin, Italy), in 2008, and in the Microsystem Technology Lab at KTH - Royal Institute of Technology (Stockholm, Sweden), in 2009. He pursued his master's thesis project as part of The Molecular Foundry at the Lawrence Berkeley National Lab (Berkeley, California). Matteo has been a Doctoral Assistant as part of the Laboratory of Microsystems LMIS2 at EPFL since May 2011. He obtained his PhD in Microsystems and Microelectronics in 2015 with his thesis titled: "Merging microfluidics and micro-array concepts: from molecular to nematode-based bioassays".

Supervised projects

- Gopal Krishnamani - MSc in Life Sciences - EPFL. Research Projects
- Federico Cantoni - MSc in Material Engineering, ENSIACET Toulouse. Master internship
- Chiara Rossi - MSc in Nanotechnologies - EPFL/GrenobleINP/PoliTo. Summer internship
- Edoardo Ceccarelli - MSc in Nanotechnologies - EPFL/GrenobleINP/PoliTo. Summer internship
- Daniel Migliozzi - MSc in Life Sciences - EPFL. Master Thesis
- Marie-Pierre Meurville - Bs in Life Sciences - EPFL. Bachelor Project
- Alexis Marette - MSc in Nanotechnologies - EPFL/GrenobleINP/PoliTo. Master Thesis
- Dimitry Dumont-Fillon - MSc in Nanotechnologies - EPFL/GrenobleINP/PoliTo. Summer internship
- Shreya Narasimhan - Bs in Life Sciences - EPFL. Semester Project

Education

PhD	Microsystems and Microelectronics	EPFL	2011-2015
M.Sc.	Nanotechnologies	EPFL / Grenoble INP / PoliTo	2008-2010
B.S.	Physics engineering	Polytechnic University of Turin (PoliTo)	2005-2008

Publications

Journal Papers

M. Cornaglia, G. Krishnamani, L. Mouchiroud, V. Sorrentino, T. Lehnert, J. Auwerx and M. A. M. Gijs, Automated longitudinal monitoring of in vivo protein aggregation in neurodegenerative disease C. elegans models, Mol Neurodegener 11, doi: 10.1186/s13024-016-0083-6 (2016) link L. Dong, M. Cornaglia, T. Lehnert, and M. A. M. Gijs, Versatile size-dependent sorting of C. elegans nematodes and embryos using a tunable microfluidic filter structure, Lab Chip 16, 574-585, doi: 10.1039/C5LC01328C (2015) link M. Cornaglia, L. Mouchiroud, A. Marette, S. Narasimhan, T. Lehnert, V. Jovaisaite, J. Auwerx, and M. A. M. Gijs, An automated microfluidic platform for C. elegans embryo arraying, phenotyping, and long-term live imaging, Sci Rep 5, 10192, doi: 10.1038/srep10192 (2015) link M. Cornaglia, R. Trouillon, H. C. Tekin, T. Lehnert, and M. A. M. Gijs, Dose-response curve of a microfluidic magnetic bead-based surface coverage sandwich assay, New Biotechnol 32, 433-440, doi: 10.1016/j.nbt.2015.03.008 (2015) link H. Yang, M. Cornaglia, and M. A. M. Gijs, Photonic Nanojet Array for Fast Detection of Single Nanoparticles in a Flow. Nano Lett, doi: 10.1021/nl5044067 (2015) link P. Tewari Kumar, K. Vriens, M. Cornaglia, M. A. M. Gijs, T. Kokalj, K. Thevissen, A. Geeraerd, B.P.A. Cammue, R. Puers and J. Lammertyn, Digital microfluidics for time-resolved cytotoxicity studies on single non-adherent yeast cells. Lab Chip, doi: 10.1039/C4LC01469C (2015) link M. Cornaglia, R. Trouillon, H. C. Tekin, T. Lehnert, and M. A. M. Gijs, Magnetic particle-scanning for ultrasensitive immunodetection on-chip. Anal Chem 86, 8213-8223, doi:10.1021/ac501568g (2014) link B. Verbruggen, T. Tóth, M. Cornaglia, R. Puers, M. A. M. Gijs, and J. Lammertyn. Separation of magnetic microparticles in segmented flow using asymmetric splitting regimes. Microfluid Nanofluid 18, 91-102, doi: 10.1007/s10404-014-1409-8 (2014) link N. Vergauwe, S. Vermeir, J. B. Wacker, F. Ceyssens, M. Cornaglia, R. Puers, M. A. M. Gijs, J. Lammertyn, and D. Witters. A highly efficient extraction protocol for magnetic particles on a digital microfluidic chip. Sensor Actuat B-Chem 196, 282-291, doi:Doi 10.1016/j.snb.2014.01.076 (2014) link M. Cornaglia, H. C. Tekin, T. Lehnert & M. A. M. Gijs. Fine-tuning of magnetic and microfluidic viscous forces for specific magnetic bead-based immunocomplex formation. J Appl Phys 114, doi: 10.1063/1.4817663 (2013) link H. C. Tekin, M. Cornaglia & M. A. M. Gijs. Attomolar protein detection using a magnetic bead surface coverage assay. Lab Chip 13, 1053-1059, doi: 10.1039/C3lc41285g (2013) link A. Weber-Bargioni, A. Schwartzberg, M. Cornaglia, A. Ismach, J. J. Urban, Y. J. Pang, R. Gordon, J. Bokor, M. B. Salmeron, D. F. Ogletree, P. Ashby, S. Cabrini & P. J. Schuck. Hyperspectral Nanoscale Imaging on Dielectric Substrates with Coaxial Optical Antenna Scan Probes. Nano Lett 11, 1201-1207, doi: 10.1021/Nl104163m (2011) link C. F. Carlborg, T. Haraldsson, M. Cornaglia, G. Stemme & W. van der Wijngaart. A High-Yield Process for 3-D Large-Scale Integrated Microfluidic Networks in PDMS. J Microelectromech S 19, 1050-1057, doi: 10.1109/Jmems.2010.2067203 (2010) link

Conference Papers

M. C. Letizia, M. Cornaglia, and M.A.M. Gijs, Accurate quantification of bacteria concentration as a nutrient for C. elegans worm culture on chip. NanoBioTech 2015, Montreux, Switzerland, November 2015. Poster presentation M. Cornaglia, L. Mouchiroud, A. Marette, S. Narasimhan, T. Lehnert, V. Jovaisaite, J. Auwerx, and M. A. M. Gijs, Multi-dimensional imaging and phenotyping of C. elegans embryos via an automated microfluidic device. Proc. of 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2015), Gyeongju, Korea, October 2015. Poster presentation M. Cornaglia, G. Krishnamani, M. Meurville, L. Mouchiroud, T. Lehnert, J. Auwerx, and M.A.M. Gijs. A microfluidic device for longitudinal studies of C. elegans neurodegenerative models. Proc. of 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2015), Gyeongju, Korea, October 2015. Poster presentation H. Yang, M. Cornaglia, and M.A.M. Gijs, Fast detection of single nanoparticles in a microfluidic channel by a microlens array in combination with conventional optical microscope. Proc. of 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2015), Gyeongju, Korea, October 2015. Poster presentation G. Huszka, H. Yang, M. Cornaglia, and M.A.M. Gijs, Patterning of melamine and SiO2 microsphere lens arrays. MNE 2015 - the 41th International Conference on Micro and Nano Engineering, The Hague, The Netherlands, September 2015. Poster presentation M. Cornaglia, L. Mouchiroud, J. Auwerx, and M.A.M. Gijs, Automated microfluidic plat-forms for C. elegans arraying, phenotyping, and long-term analysis. BioInnovation Day 2015, CHUV, Lausanne, Switzerland, June 2015. Oral presentation G. Krishnamani, M. Cornaglia, L. Mouchiroud, M.A.M. Gijs, and J. Auwerx, Long-term culture and high-resolution imaging of C. elegans using an automated microfluidic platform. Third Lausanne Integrative Metabolism and Nutrition Alliance (LIMNA) Symposium, Lau-sanne, Switzerland, March 2015. Poster presentation H. Yang, M. Cornaglia, T. Lehnert and M.A.M. Gijs, Study of constrained Brownian motion of nanoparticles near an interface using optical tweezers . Proc. of SPIE, Vol. 9379, Photonics West, San Francisco, CA, USA, February 2015. Poster presentation P. Tewari Kumar, K. Vriens, M. Cornaglia, M.A.M. Gijs, T. Kokalj, K. Thevissen, A. Geeraerd, B.P.A.Cammue, R. Puers, and J. Lammertyn. Digital microfluidic chip technology for cytotoxicity studies on yeast cells at spatio-temporal resolution. NanoBioTech 2014, Montreux, Switzerland, November 2014. Oral presentation M. Cornaglia, G. Krishnamani, M. Meurville, L. Mouchiroud, T. Lehnert, J. Auwerx, and M.A.M. Gijs. An automated microfluidic platform for long-term high-resolution imaging of C. elegans. Proc. of 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2014), San Antonio, TX, USA, October 2014. Poster presentation M. Cornaglia, R. Trouillon, H.C. Tekin, T. Lehnert, and M.A.M. Gijs. A random-walk based model to explain ultrasensitive magnetic bead-based immunoassays. Proc. of 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2014), San Antonio, TX, USA, October 2014. Oral presentation H. Yang, M. Cornaglia, T. Lehnert, and M.A.M. Gijs. Lens array by electrostatic patterning of dielectric microspheres in a parylene-c well template. Proc. of 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2014), San Antonio, TX, USA, October 2014. Poster presentation B. Verbruggen, F. Ceyssens, K. Leirs, M. Cornaglia, M.A.M. Gijs, T. Kokalj, R. Puers, and J. Lammertyn. Magnetic microparticle based DNA extraction in a droplet microfluidic chip. Proc. of 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2014), San Antonio, TX, USA, October 2014. Poster presentation P. Tewari Kumar, K. Vriens, M. Cornaglia, M.A.M. Gijs, T. Kokalj, K. Thevissen, A. Geeraerd, B.P.A. Cammue, R. Puers, and J. Lammertyn. Spatio-temporal analysis of yeast cells responses on a digital microfluidic platform. Diatech 2014 - Novel technologies for in vitro diagnostics, Leuven, Belgium, October 2014. Poster presentation M. Cornaglia, R. Trouillon, H.C. Tekin, T. Lehnert, and M.A.M. Gijs. Random-walk model of magnetic bead-based surface coverage immunoassays. Diatech 2014 - Novel technologies for in vitro diagnostics, Leuven, Belgium, October 2014. Oral presentation B. Verbruggen, F. Ceyssens, K. Leirs, M. Cornaglia, M.A.M. Gijs, T. Kokalj, R. Puers, and J. Lammertyn. DNA extraction with magnetic microbeads in segmented flow microfluidic chip. Diatech 2014 - Novel technologies for in vitro diagnostics, Leuven, Belgium, October 2014. Poster presentation M. Cornaglia, H. Yang, T. Lehnert, and M.A.M. Gijs. Electrostatic self-assembly of microsphere lens arrays. MNE 2014 - the 40th International Conference on Micro and Nano Engineering, Lausanne, Switzerland, September 2014. Poster presentation M. Cornaglia, G. Krishnamani, M. Meurville, L. Mouchiroud, T. Lehnert, J. Auwerx, and M.A.M. Gijs. An automated microfluidic platform for reversible immobilization and imaging of C. elegans. EMBL Microfluidics 2014, Heidelberg, Germany, July 2014. Poster presentation M. Cornaglia, H.C. Tekin, T. Lehnert, M.A.M. Gijs. Magnetic Bead-Rolling for Ultrasensitive Surface-Based Immunoassays. Proc. of 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2013), October 2013, Freiburg, Germany. Oral presentation M. Cornaglia, H.C. Tekin, T. Lehnert, M.A.M. Gijs. Balancing Magnetic Dipolar and Viscous Forces on Superparamagnetic Beads for Improving the Specificity of Microfluidic Surface-based Immunoassays. 9th International Conference on the Scientific and Clinical Applications of Magnetic Carriers, Minneapolis, Minnesota, USA, May 2012. Poster presentation M. Cornaglia, H.C. Tekin, T. Lehnert, M.A.M. Gijs. A Combined Finite Element Method/Analytical Model of the Magnetophoretic Manipulation of Superparamagnetic Particles for Microfluidic Immunoassays. EMBL Microfluidics 2012, Heidelberg, Germany, July 2012. Poster presentation H.C. Tekin, M. Cornaglia, C. Razaname, M.A.M. Gijs. Analysis of protein biomarkers via magnetic bead surface coverage detection. NanoBioTech 2011, Montreux, Switzerland, November 2011. Poster presentation A. Weber-Bargioni, A. Schwartzberg, M. Cornaglia, A. Ismach, J. J. Urban, Y. J. Pang, R. Gordon, D. F. Ogletree, S. Cabrini & P. J. Schuck. Hyperspectral Nanoscale Imaging on Dielectric Substrates with Coaxial Optical Antenna Scan Probes. 2011 Conference on Lasers and Electro-Optics (Cleo) (2011) M. Cornaglia, A. Weber-Bargioni, S. Dhuey, A. McLeod, J. Neaton, D.F. Ogletree, P.J. Schuck, S. Cabrini. Manipulating Resonance and Radiative Properties of Optical Bowtie Antennae by Modifying the Antennas Aspect Ratio while Maintaining the Enhancement. MNE - International Conference on Micro & Nano Engineering, Genova, Italy, September 2010, Oral presentation C. F. Carlborg, K. T. Haraldsson, M. Cornaglia, G. Stemme & W. van der Wijngaart. Large Scale Integrated 3d Microfluidic Networks through High Yield Fabrication of Vertical Vias in Pdms. Proc Ieee Micr Elect, 240-243 (2010)