Fields of expertise
Solid State Nanopores
Second Harmonic Generation
Super -resolution microscopy
Curriculum VitaeCurriculum Vitae CV
MissionMany biomolecules show remarkably more complex behavior at the single-molecule level than it is observed in bulk, ensemble-averaged experiments. It is now evident that sparsely populated molecular sub-states are vital for the biological function. Insights into complex behavior can be gained through manipulation, imaging or sensing of single biomolecules.
Single-molecule techniques can provide us with extraordinarily clear and often surprising views of biomolecules in action. Development of appropriate instrumentation has been identified as the key ingredient for advances in biophysical sciences. Three most important examples here are single molecule localization microscopy (SMLM), optical tweezers and nanopores. SMLM made it possible to see by visualizing single fluorophores in living cells while laser-based optical tweezers allowed us to touch with experiments on physical forces involved in stretching and binding of biomolecules, while nanopores, the simplest and the most recent single molecule technique allows for rapid and high-throughput biosensing and discrimination of attomolar molecule concentrations.
BiographyFrom April 2021 Full Professor
2015 -2021 Associate Professor
2008-2015 Tenure-Track Assistant Professor
2004-2007 Postdoc at the University of California, Berkeley in the group of Prof.Liphardt
2003 PhD student of Prof. Dietler in Laboratory of Physics of Living Matter, University of Lausanne
1999 Diploma thesis on the subject of the Raman spectroscopy of beta carotene
1994-1999 Physics department at the University of Zagreb
1994 baccalaureate, Classical gymnasium
Current workMy lab works in the research field that can be termed single molecule biophysics. We develop techniques and methodologies based on optical imaging, biosensing and single molecule manipulation with the aim to monitor the behavior of individual biological molecules and complexes in vitro and in live cells. Our current research is focused on three major directions:
(i) Developing and using nanopores as platform for molecular sensing and manipulation. In particular we focus on solid-state nanopores realized either in glass nanocapillaries, or on suspended 2d-material membranes and standard silicon-nitride membranes.
(ii) Studying how biomolecules function, especially how proteins and nucleic acids interact, using force-based manipulation single-molecule techniques, in particular optical tweezers, optical wrench system, Anti- Brownian Electrokinetic (ABEL) trap and combination of nanopore/nanocapillaries with OT.
(iii) Developing super-resolution optical microscopy, based on single molecule localizations (SMLM) in cells with molecular-scale resolution, with an aim to extract quantitative information.
Awards/Achievements2016 CCMX Materials challenge award
2015 SNSF-ERC Consolidator Grant
2010 ERC Starting Grant
2003 Fellowship of the Swiss National Science Foundation
PatentsPatent Number: WO07079411
ALIGNMENT, TRANSPORTATION AND INTEGRATION OF NANOWIRES USING OPTICAL TRAPPING
Publication date: 2007-07-12.
MANUFACTURING OF ORIFICES IN GLASS LIKE MATERIALS, E.G. NANOCAPILLARIES AND OBJECT OBTAINED ACCORDING TO THIS PROCESS
Publication date: 2014-25-03
Link US patent
Patent Number: WO2015121394
MOLECULAR SENSING DEVICE-
Publication date: 2015-20-08
Patent Number: WO2016142925A1
NANOPORE FORMING METHOD AND USES THEREOF WO2016142925A1
Publication date :2016-15-09
Patent Number: WO2018002099 OSMOTIC POWER GENERATION IN 2D NANOPORES
Publication date :2018-15-09
Publication date :2016-15-09 Link Link US patent
Patent application:BENCHMARKING OF SINGLE IMAGING DATASETS19 180 900.3 - Your ref.: 6.1943 - Our ref.: 34623EP Patent applicationSYSTEMS AND METHODS FOR DIGITAL INFORMATION DECODING AND DATA STORAGE IN HYBRID MACROMOLECULES
ERC Advanced Grant
CCMX Materials challenge award
SNSF-ERC Consolidator Grant
ERC Starting Grant
Peer reviewed journal articles
 Three-step, transfer-free growth of MoS2/WS2/graphene vertical van der Waals heterostructure2D Materials. 2022-04-01. DOI : 10.1088/2053-1583/ac5f6d.
 Engineering Optically Active Defects in Hexagonal Boron Nitride Using Focused Ion Beam and WaterAcs Nano. 2022-03-22. DOI : 10.1021/acsnano.1c07086.
 Zero-Bias Power-Detector Circuits based on MoS2 Field-Effect Transistors on Wafer-Scale Flexible SubstratesAdvanced Materials. 2022-02-17. DOI : 10.1002/adma.202108469.
 Time-Resolved Scanning Ion Conductance Microscopy for Three-Dimensional Tracking of Nanoscale Cell Surface DynamicsAcs Nano. 2021-11-23. DOI : 10.1021/acsnano.1c05202.
 Rhesus Blood Typing within a Few Seconds by Packing-Enhanced Nanoscattering on Individual ErythrocytesAnalytical Chemistry. 2021-11-16. DOI : 10.1021/acs.analchem.1c03590.
 Anomalous interfacial dynamics of single proton charges in binary aqueous solutionsScience Advances. 2021-10-01. DOI : 10.1126/sciadv.abg8568.
 Bio-orthogonal Red and Far-Red Fluorogenic Probes for Wash-Free Live-Cell and Super-resolution MicroscopyAcs Central Science. 2021-09-22. DOI : 10.1021/acscentsci.1c00703.
 Experimental Combination of Super-Resolution Optical Fluctuation Imaging with Structured Illumination Microscopy for Large Fields-of-ViewAcs Photonics. 2021-08-18. DOI : 10.1021/acsphotonics.1c00668.
 Direct Growth of Hexagonal Boron Nitride on Photonic Chips for High-Throughput CharacterizationAcs Photonics. 2021-07-21. DOI : 10.1021/acsphotonics.1c00165.
 High resolution optical projection tomography platform for multispectral imaging of the mouse gutBiomedical Optics Express. 2021-06-01. DOI : 10.1364/BOE.423284.
 From Water Solutions to Ionic Liquids with Solid State Nanopores as a Perspective to Study Transport and Translocation PhenomenaSmall. 2021-05-06. DOI : 10.1002/smll.202100777.
 Super-resolved Optical Mapping of Reactive Sulfur-Vacancies in Two-Dimensional Transition Metal DichalcogenidesAcs Nano. 2021-04-27. DOI : 10.1021/acsnano.1c00373.
 Wetting of nanopores probed with pressurePhysical Chemistry Chemical Physics. 2021-02-28. DOI : 10.1039/d1cp00253h.
 Decoding Digital Information Stored in Polymer by Nanopore2021-02-12. p. 98A-98A.
 Recent Advances and Prospects in the Research of Nascent AdhesionsFrontiers In Physiology. 2020-12-04. DOI : 10.3389/fphys.2020.574371.
 Towards artificial mechanosensingNature Materials. 2020-10-01. DOI : 10.1038/s41563-020-00811-5.
 Self-Blinking Dyes Unlock High-Order and Multiplane Super-Resolution Optical Fluctuation ImagingAcs Nano. 2020-07-28. DOI : 10.1021/acsnano.0c04602.
 Waveguide-Based Platform for Large-FOV Imaging of Optically Active Defects in 2D MaterialsAcs Photonics. 2019-12-01. DOI : 10.1021/acsphotonics.9b01103.
 Spatiotemporal Imaging of Water in Operating Voltage-Gated Ion Channels Reveals the Slow Motion of Interfacial IonsNano Letters. 2019-10-03. DOI : 10.1021/acs.nanolett.9b02024.
 Wafer-scale MOCVD growth of monolayer MoS2 on sapphire and SiO2Nano Research. 2019-10-01. DOI : 10.1007/s12274-019-2502-9.
 Facile Production of Hexagonal Boron Nitride Nanoparticles by Cryogenic ExfoliationNano Letters. 2019-08-01. DOI : 10.1021/acs.nanolett.9b01913.
 Transverse Detection of DNA in a MoS2 NanoporeBiophysical Journal. 2018. DOI : 10.1016/j.bpj.2017.11.1005.
 Fluorescent Nanodiamonds in Biological and Biomedical Imaging and SensingSuper-Resolution Imaging in Biomedicine; Taylor & Francis Group, 6000 Broken Sound Parkway NW, Suite 300, Boca Raton, FL 33487-2742: CRC Press, 2016.
 Molybdenum Disulfide Nanopores: Why 3 Atoms are Better than One?2015. 59th Annual Meeting of the Biophysical-Society, Baltimore, MD, FEB 07-11, 2015. p. 489A-489A.
 Investigating Cellular Focal Adhesions on Nano-Patterned Substrates with Dual Color Photo-Activated Localization Microscopy2015. 59th Annual Meeting of the Biophysical-Society, Baltimore, MD, FEB 07-11, 2015. p. 359A-359A.
 Electron Spin Resonance of Nitrogen-Vacancy Defects Embedded in Single Nanodiamonds in an ABEL TrapNano Letters. 2014. DOI : 10.1021/nl5023964.
 Shrinking Nanocapillaries to Low Noise Nanopores for Single Molecule Detection2014. 58th Annual Meeting of the Biophysical-Society, San Francisco, CA, FEB 15-19, 2014. p. 633A-633A.
 Combination of Optical Tweezers with Nanocapillaries as System for Estimation of DNA/Ligand Interactions2014. 58th Annual Meeting of the Biophysical-Society, San Francisco, CA, FEB 15-19, 2014. p. 393A-393A.
 Niobates Nanowires: Synthesis, Characterization and ApplicationsNanowires - Implementations and Applications; In Tech, 2011. p. 509-524.
 Electrophoretic threading kinetics of optically trapped DNA through synthetic nanoporesBiophysical Journal. 2007.
 Complex characterization of physiology solution based magnetic fluidIndian Journal of Engineering and Materials Sciences. 2004.
 Study of probes and substrates for low temperature atomic force microscopy and biological applications2003.
Teaching & PhD
Life Sciences Engineering
Doctoral Program in Biotechnology and Bioengineering
Doctoral Program in Photonics
Doctoral Program in Chemistry and Chemical Engineering
Doctoral Program in Materials Science and Engineering
Doctoral Program in Computational and Quantitative Biology
Doctoral Program in Microsystems and Microelectronics
Doctoral Program in Physics