Aleksandra Radenovic
Full Professor
aleksandra.radenovic@epfl.ch +41 21 693 73 71 http://lben.epfl.ch
Citizenship: Croatian and Swiss
Birth date: 27.07.1975
EPFL STI IBI-STI LBEN
BM 2140 (Bâtiment BM)
Station 17
CH-1015 Lausanne
+41 21 693 73 71
+41 21 693 11 61
Office:
BM 2140
EPFL
>
STI
>
IBI-STI
>
LBEN
Web site: Web site: https://lben.epfl.ch/
+41 21 693 73 71
EPFL
>
SV
>
SV-SSV
>
SSV-ENS
Web site: Web site: https://sv.epfl.ch/education
EPFL P-SG AE
CE 2 328 (Centre Est)
Station 1
CH-1015 Lausanne
Web site: Web site: https://ae.epfl.ch/
EPFL CIME-CD
MXC 330 (Bâtiment MXC)
Station 12
CH-1015 Lausanne
+41 21 693 73 71
Office:
MXC 330
EPFL
>
VPA
>
VPA-AVP-CP
>
CIME
>
CIME-CD
+41 21 693 73 71
EPFL
>
STI
>
STI-SMT
>
SMT-ENS
+41 21 693 73 71
EPFL
>
VPA
>
VPA-FAC
>
CEAE
Fields of expertise
Solid State Nanopores
2-D materials
Optical tweezers
Second Harmonic Generation
Super -resolution microscopy
Signaling
Curriculum Vitae
Curriculum Vitae CVMission
Many biomolecules show remarkably more complex behavior at the single-molecule level than it is observed in bulk, ensemble-averaged experiments. It is now evident that sparsely populated molecular sub-states are vital for the biological function. Insights into complex behavior can be gained through manipulation, imaging or sensing of single biomolecules.Single-molecule techniques can provide us with extraordinarily clear and often surprising views of biomolecules in action. Development of appropriate instrumentation has been identified as the key ingredient for advances in biophysical sciences. Three most important examples here are single molecule localization microscopy (SMLM), optical tweezers and nanopores. SMLM made it possible to see by visualizing single fluorophores in living cells while laser-based optical tweezers allowed us to touch with experiments on physical forces involved in stretching and binding of biomolecules, while nanopores, the simplest and the most recent single molecule technique allows for rapid and high-throughput biosensing and discrimination of attomolar molecule concentrations.
Biography
From April 2021 Full Professor2015 -2021 Associate Professor
2008-2015 Tenure-Track Assistant Professor
2004-2007 Postdoc at the University of California, Berkeley in the group of Prof.Liphardt
2003 PhD student of Prof. Dietler in Laboratory of Physics of Living Matter, University of Lausanne
1999 Diploma thesis on the subject of the Raman spectroscopy of beta carotene
1994-1999 Physics department at the University of Zagreb
1994 baccalaureate, Classical gymnasium
Current work
My lab works in the research field that can be termed single molecule biophysics. We develop techniques and methodologies based on optical imaging, biosensing and single molecule manipulation with the aim to monitor the behavior of individual biological molecules and complexes in vitro and in live cells. Our current research is focused on three major directions:(i) Developing and using nanopores as platform for molecular sensing and manipulation. In particular we focus on solid-state nanopores realized either in glass nanocapillaries, or on suspended 2d-material membranes and standard silicon-nitride membranes.
(ii) Studying how biomolecules function, especially how proteins and nucleic acids interact, using force-based manipulation single-molecule techniques, in particular optical tweezers, optical wrench system, Anti- Brownian Electrokinetic (ABEL) trap and combination of nanopore/nanocapillaries with OT.
(iii) Developing super-resolution optical microscopy, based on single molecule localizations (SMLM) in cells with molecular-scale resolution, with an aim to extract quantitative information.
Awards/Achievements
2016 CCMX Materials challenge award2015 SNSF-ERC Consolidator Grant
2010 ERC Starting Grant
2003 Fellowship of the Swiss National Science Foundation
Patents
Patent Number: WO07079411ALIGNMENT, TRANSPORTATION AND INTEGRATION OF NANOWIRES USING OPTICAL TRAPPING
Publication date: 2007-07-12.
Link
Patent Number:PCT/IB2013/052093
MANUFACTURING OF ORIFICES IN GLASS LIKE MATERIALS, E.G. NANOCAPILLARIES AND OBJECT OBTAINED ACCORDING TO THIS PROCESS
Publication date: 2014-25-03
Link US patent
Patent Number: WO2015121394
MOLECULAR SENSING DEVICE-
Publication date: 2015-20-08
Link
Patent Number: WO2016142925A1
NANOPORE FORMING METHOD AND USES THEREOF WO2016142925A1
Publication date :2016-15-09
Link
Patent Number: WO2018002099 OSMOTIC POWER GENERATION IN 2D NANOPORES
Publication date :2018-15-09
Publication date :2016-15-09 Link Link US patent
Patent application:BENCHMARKING OF SINGLE IMAGING DATASETS19 180 900.3 - Your ref.: 6.1943 - Our ref.: 34623EP Patent applicationSYSTEMS AND METHODS FOR DIGITAL INFORMATION DECODING AND DATA STORAGE IN HYBRID MACROMOLECULES
2020 September
Awards
ERC Advanced Grant
2021
CCMX Materials challenge award
2016
SNSF-ERC Consolidator Grant
2015
ERC Starting Grant
2010
SNSF Fellowship
2003
Publications
Infoscience publications
Teaching & PhD
Teaching
Life Sciences Engineering
Microengineering