Florence Pojer

Nationalité: Swiss-French

EPFL SV PTECH PTPSP
AI 2147 (Bâtiment AI)
Station 19
1015 Lausanne

Expertise

Head of a core facility within the Life Sciences School - PTPSP
With main expertise in Integrative protein Structural Biology (e.g. biophysical techniques, X-ray Crystallography, SPR Cryo-EM,..)
And
production/Purification of macromolecules (e.g. antibodies, membrane proteins, growth factors...) expressed in mammalian, insect and bacterial cells

Expertise

Head of a core facility within the Life Sciences School - PTPSP
With main expertise in Integrative protein Structural Biology (e.g. biophysical techniques, X-ray Crystallography, SPR Cryo-EM,..)
And
production/Purification of macromolecules (e.g. antibodies, membrane proteins, growth factors...) expressed in mammalian, insect and bacterial cells

Mission

PTPSP is a hub for Protein Sciences and Integrative Structural Biology; with top-notch instrumentations, close connections to high-end facilities and a dedicated team of experts highly enthusiastic to help your project move forward.
On a daily basis, the team advises, supports and trains scientists in production, purification and biophysical/structural characterisations of macromolecules, such as enzymes, antibodies or any kind of proteins you are interested in.

Formation

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1999 – 2003 Tuebingen (Germany)
Dirigée par Prof. Lutz Heide

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1992 – 1998 Besancon (France)

Expériences professionnelles

Palmitoylated acyl protein thioesterase APT2 deforms membranes to extract substrate acyl chains

L. AbramiM. AudagnottoS. HoM. J. MarcaidaF. S. Mesquita  et al.

Nature Chemical Biology. 2021. DOI : 10.1038/s41589-021-00753-2.

Inhibition Mechanisms of Indoleamine 2,3-Dioxygenase 1 (IDO1)

U. F. RohrigA. ReynaudS. R. MajjigapuP. VogelF. Pojer  et al.

Journal Of Medicinal Chemistry. 2019. DOI : 10.1021/acs.jmedchem.9b00942.

The cysteine desulfurase IscS of Mycobacterium tuberculosis is involved in iron sulfur cluster biogenesis and oxidative stress defence

J. RybnikerF. PojerJ. MarienhagenG. S. KollyJ. M. Chen  et al.

Biochemical Journal. 2014. DOI : 10.1042/Bj20130732.

Pyridomycin bridges the NADH- and substratebinding pockets of the enoyl reductase InhA

R. C. HartkoornF. PojerJ. A. ReadH. GingellJ. Neres  et al.

Nature Chemical Biology. 2014. DOI : 10.1038/nchembio.1405.

Towards anti-virulence drugs targeting ESX-1 mediated pathogenesis of Mycobacterium tuberculosis

J. M. ChenF. PojerB. BlascoS. T. Cole

Drug Discovery Today: Disease Mechanisms. 2010. DOI : 10.1016/j.ddmec.2010.09.002.

Enseignement et PhD

Cours

Concept to early-stage drug and medTech products

BIO-497

Ce cours explore la mise sur le marché de médicaments et dispositifs médicaux innovants. Des experts d'entreprises leaders partagent leur expérience. Les étudiants acquièrent une compréhension pratique des aspects cliniques, réglementaires et de qualité.

Methods: from disease models to therapy

BIOENG-518

Ce cours décrira les méthodes qui sont à la base des approches translationnelles depuis la génération et la caractérisation des modèles des maladies jusqu'aux applications thérapeutiques. Les bases théoriques seront complétées par des rotations pratiques dans les plateformes technologiques.

Methods: omics in biomedical research

BIOENG-519

Les méthodologies à haut débit, appelées "Omiques", permettent de caractériser la complexité et la dynamique de tout système biologique. Ce cours fournira une description générale des méthodes liées au domaine des "Omiques", suivie de rotations pratiques dans les plateformes participantes.

Open and Outreach sciences

PTPSP is regularly hosting kids and classes to discuss scientific topics and propose practical experiments. PTPSP is also participating in EPFL open days.