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Administrative data

Suliana Manley

suliana.manley@epfl.ch +41 21 693 06 32 http://leb.epfl.ch/

EPFL SB IPHYS LEB
BSP 427 (Cubotron UNIL)
Rte de la Sorge
1015 Lausanne

Mission

Cellular responses to external signals begin at the plasma membrane, where the dynamic assembly of receptors or ion channels can regulate cellular activity. Membrane-enveloped viruses, including the human immunodeficiency virus (HIV) also assemble at the plasma membrane, exploiting mechanisms evolved for cellular trafficking. Despite the importance of these processes, our physical paradigm for how proteins form mesoscale assemblies is far from complete.
This is in part a consequence of technical limitations. While the organization and dynamics of membrane proteins are heterogeneous, commonly used fluorescence-based measurements lack information at the molecular scale. In contrast, single molecule measurements limited to looking at only a few molecules in a given cell lack ensemble information. Thus, the study of protein assembly has been limited by a lack of spatially resolved, dynamic information on ensembles of molecules. To overcome these obstacles, we use super-resolution fluorescence imaging techniques combined with live cell imaging and single molecule tracking to determine how the dynamics of protein assembly are coordinated.
From 2016 Associate professor, EPFL, Lausanne, Switzerland 2009-2016 Tenure-track assistant professor, EPFL, Lausanne, Switzerland 2006-2009 Post-Doctoral fellow, National Institutes of Health, Bethesda, MD, USA 2004-2006 Post-Doctoral fellow, Massachusetts Institute of Technology, Cambridge, MA, USA 1999-2004 PhD (Physics) Awarded 06/2004, Harvard University, Cambridge, MA, USA 1993-1997 Bachelors (Cum Laude) Physics & Mathematics, Rice University, Houston, TX, USA

POSITIONS AVAILABLE

PhD and postdoctoral positions available. Email for more information.

RECENT PUBLICATIONS

Manoël Prouteau, Ambroise Desfosses, Christian Sieben, Clélia Bourgoint, Nour Lydia Mozaffari, Davide Demurtas, Alok K. Mitra, Paul Guichard, Suliana Manley and Robbie Loewith , «TORC1 Organised in Inhibited Domains (TOROIDs) regulate TORC1 activity,» Nature doi:10.1038/nature24021 (2017) A Vancevska*, KM Douglass*, V Pfeiffer, S Manley and J Lingner , «The telomeric DNA damage response occurs in the absence of chromatin decompaction,» Genes Dev, 31(6):567-577 (2017) T Verdier, J Gunzenhäuser, S Manley and M Castelnovo , "Single Particle Maximum Likelihood Reconstruction from Superresolution Microscopy Images," PLoS One, 12(3), e0172943 (2017) M Bergé, S Campagne, J Mignolet, SJ Holden, L Théraulaz, S Manley, FHT Allain and PH Viollier , "Modularity and determinants of a (bi-)polarization control system from free-living and obligate intracellular bacteria," eLife 5:e20640 (2016) J Mignolet, SJ Holden, M Bergé, G Panis, L Théraulaz, S Manley and PH Viollier , "Functional dichotomy and distinct nanoscale assemblies of a cell cycle-controlled bipolar zinc-finger regulator," eLife 5:e18647 (2016) C Deluz, ET Friman, D Strebinger, A Benke, M Raccaud, A Callegari, S Manley and DM Suter , "A role for mitotic bookmarking of SOX2 in pluripotency and differentiation," Genes Dev 30(22) 2538-2550 (2016) K Douglass , C Sieben, A Archetti, A Lambert, S Manley , "Super-resolution imaging of multiple cells by optimized flat-field epi-illumination," Nat Photonics 10.1038 (2016) Featured in Nat Methods Research Highlights Y Nasu, A Benke, S Arakawa, GJ Yoshida, G Kawamura, S Manley, S Shimizu, T Ozawa , "In Situ Characterization of Bak Clusters Responsible for Cell Death Using Single Molecule Localization Microscopy," Sci Rep 6:27505 (2016) L Carlini*, S Holden*, K Douglass* and S Manley , "Correction of a depth-dependent lateral distortion in 3D super-resolution imaging," PLoS One 10(11) e0142949 (2015) PJ Fabre, A Benke, E Joye, THN Huynh, S Manley and D Duboule , "Nanoscale spatial organization of the HoxD cluster in distinct transcriptional states," Proc Natl Acad Sci USA 112(45) 13964-13969 (2015) D Sage, H Kirschner, T Pengo, N Stuurman, J Min, S Manley and M Unser , "Quantitative evaluation of software packages for single-molecule localization microscopy." Nat Methods,12(8) 717-724 (2015) T Pengo, S Holden and S Manley , "PALMsiever: a tool to turn raw data into results for single-molecule localization microscopy," Bioinformatics, 31(5):797-798 (2015) J Gunzenhäuser, R Wyss and S Manley , "A quantitative approach to evaluate the impact of fluorescent labeling on HIV-Gag assembly by titration of unlabeled proteins," PLoS One, 9(12) e115095 (2014) J Min*, S Holden*, L Carlini, M Unser, S Manley and JC Ye , "3D high-density localization microscopy using hybrid astigmatic/ biplane imaging and sparse image reconstruction," Biomed Opt Express, 5(11):3935-3948 (2014) J Min, C Vonesch*, H Kirschner*, L Carlini*, N Olivier, S Holden, S Manley, JC Ye and M Unser , "FALCON: Fast and unbiased reconstruction of high-density super-resolution microscopy data," Sci Rep 4:4577 (2014) SJ Holden, T Pengo, K Meibom, C Fernandez-Fernandez, J Collier and S Manley , "In vivo quantitative analysis of Caulobacter crescentus z-ring organization by high throughput 3D PALM," Proc Natl Acad Sci USA 111(12):4566-4571 (2014) Featured in Nat Methods Research Highlights L Carlini, A Benke, L Reymond, G Lukinavicius and S Manley , "Reduced dyes enhance single molecule localization density for live superresolution imaging," ChemPhysChem 15(4):750-755 (2014) D Keller, M Orpinell, N Olivier, M Wachsmuth, R Mahen, R Wyss, V Hachet, J Ellenberg, S Manley and P Gönczy , "Mechanisms of HsSAS-6 assembly promoting centriole formation in human cells," J Cell Biol 204(5):697-712 (2014) L Carlini and S Manley , "Live intracellular super-resolution imaging using site-specific stains," ACS Chem Biol 8(12):2643-2648 (2013) N Olivier , D Keller, P Gönczy and S Manley , "Resolution doubling in 3D STORM imaging through improved buffers," PLoS One 8(7):e69004 (2013) N Olivier , D Keller, VS Rajan, P Gönczy and S Manley , "Simple buffers for 3D STORM microscopy," Biomed Opt Express 4(6):885-899 (2013) J Min, C Vonesch, N Olivier, H Kirshner, S Manley, JC Ye and M Unser , "Continuous localization using sparsity constraints for high-density super-resolution microscopy," ISBI (2013) G Lukinavicius, K Umezawa, N Olivier, A Honigmann, G Yang, T Plass, V Mueller, L Reymond, IR Correa Jr., Z-G Luo, C Schultz, EA Lemke, P Heppenstall, C Eggeling, S Manley and K Johnsson , "A near-infrared fluorophore for live-cell superresolution microscopy of cellular proteins," Nat Chem 5(2):132-139 (2013) N Hoze, D Nair, E Hosy, C Sieben, S Manley, A Herrmann, J-B Sibarita, D Choquet and D Holcman , «Heterogeneity of AMPA receptor trafficking and molecular interactions revealed by superresolution analysis of live cell imaging,» Proc Natl Acad Sci USA, 109(42):17052-17057 (2012) J Gunzenhäuser, N Olivier, T Pengo and S Manley , «Quantitative super-resolution imaging reveals protein stoichiometry and nanoscale morphology of assembling HIV-Gag virions,» Nano Lett 12(9):4705-10 (2012) H Kirschner, T Pengo, N Olivier, D Sage, S Manley, and M Unser «A PSF-based approach to Biplane calibration in 3D super-resolution microscopy,» ISBI (2012) pdf A Benke*, N Olivier*, J Gunzenhäuser and S Manley , «Multicolor single molecule tracking of stochastically active synthetic dyes,» Nano Lett 12(5):2619-2624 (2012) A Benke and S Manley , «Live-cell dSTORM of cellular DNA based on direct DNA labeling,» ChemBioChem, 13(2):298-301 (2012) «Very Important Paper» and cover, Featured in Nat Methods Research Highlights S Banala, D Maurel, S Manley and K Johnsson «A caged, localizable rhodamine derivative for superresolution microscopy,» ACS Chem Biol, 7(2) (2012)

Awards

Medal for Innovation in Light Microscopy by the Royal Microscopy Society

2018

Fellowship to the American Physical Society

American Physical Society

2020

Teaching & PhD

PhD Students

Emine Berna Durmus, Santiago Nicolás Rodriguez Alvarez, Laila Elfeky, Alara Kiris, Hale-Seda Ivo Radoykova

Past EPFL PhD Students

Julia Gunzenhäuser, Aleksandr Benke, Lina Carlini, Andrea Callegari, Aster Louis K Vanhecke, Anna Archetti, Dora Mahecic, Timo Henry René Rey, Julius Winter, Chen Zhang, Jenny Sülzle, Sheda Ben Nejma

Courses

Biophysics : physics of the cell

PHYS-301

In this course we will study the cell (minimum unit of life) and its components. We will study several key cellular features : membranes, genomes, channels and receptors. We will apply the laws of physics to develop models to make quantitative and predictive statements.

Let's experiment

BIOENG-602

Before you step into the lab to do an experiment, you have a long list of questions: How do I design an experiment that will give a clear answer to my question? What model system should I use? What are my controls? What's an ideal sample size? How can I tell if the experiment worked?

Planning your scientific journey (EDBB)

BIOENG-605

Planning Your Scientific Journey: Being successful as a scientist requires more than acquiring knowledge and developing experimental skills. It also requires: (1) asking a good scientific question, (2) establishing a clear plan of action, and (3) seeking advice along the way.

Sharing your research

BIOENG-603

Giving a research talk (say, at a conference, to your department or in your research group meeting) is a really important part of a scientist's career. This course is designed for anyone who will be giving research-based scientific talks in the future.

Topics in biophysics and physical biology

PHYS-466

This course provides exposure to research in biophysics and physical biology, with emphasis on the nature of scientific breakthroughs, and using critical reading of scientific literature. Each week, we will discuss the research of one recipient of the Max Delbruck Prize in Biological Physics.

Writing for science

BIOENG-604

The goal of the course is to develop effective writing skills for academic and professional contexts.