Anne-Laure Mahul Mellier

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Publications
1. Levine, P.M., Galesic, A., Balana, A.T., Mahul-Mellier, A.L., Navarro, M.X., De Leon, C.A., Lashuel, H.A., and Pratt, M.R. (2019). alpha-Synuclein O-GlcNAcylation alters aggregation and toxicity, revealing certain residues as potential inhibitors of Parkinson's disease. Proceedings of the National Academy of Sciences of the United States of America 116, 1511-1519. 2. Desclous A., GruBmayer KS., Bostan E, Lukes T., Bouwens A., Sharipov A., Geissbuehler S, Mahul-Mellier AL., Lashuel HA, Leutenegger M., Lasser T. Combined Multi-Plane Tomographic Phase Retrieval and S tochastic Optical Fluctuation Imaging for 4D Cell Microscopy. Nature Photonics. 2018, 12, 165–172. 3. Ait-Bouziad, N., Lv, G., Mahul-Mellier, A.L., Xiao, S., Zorludemir, G., Eliezer, D., Walz, T., and Lashuel, H.A. (2017). Discovery and characterization of stable and toxic Tau/phospholipid oligomeric complexes. Nature communications 8, 1678. 4. Ruggeri, F.S., Mahul-Mellier, A.L., Kasas, S., Lashuel, H.A., Longo, G., and Dietler, G. (2017). Amyloid single-cell cytotoxicity assays by nanomotion detection. Cell death discovery 3, 17053. 5. Alzoubi, S., Brody, L., Rahman, S., Mahul-Mellier, A.L., Mercado, N., Ito, K., El-Bahrawy, M., Silver, A., Boobis, A., Bell, J.D., and Hajji, N. (2016). Synergy between histone deacetylase inhibitors and DNA-damaging agents is mediated by histone deacetylase 2 in colorectal cancer. Oncotarget 7, 44505-44521. 6. Benmessaoud, I.R., Mahul-Mellier, A.L., Horvath, E., Maco, B., Spina, M., Lashuel, H.A., and Forro, L. (2016). Health hazards of methylammonium lead iodide based perovskites: cytotoxicity studies. Toxicology research 5, 407-419. 7. Dikiy, I., Fauvet, B., Jovicic, A., Mahul-Mellier, A.L., Desobry, C., El-Turk, F., Gitler, A.D., Lashuel, H.A., and Eliezer, D. (2016). Semisynthetic and in Vitro Phosphorylation of Alpha-Synuclein at Y39 Promotes Functional Partly Helical Membrane-Bound States Resembling Those Induced by PD Mutations. ACS chemical biology 11, 2428-2437. 8. Mahul-Mellier, A.L., Vercruysse, F., Maco, B., Ait-Bouziad, N., De Roo, M., Muller, D., and Lashuel, H.A. (2015). Fibril growth and seeding capacity play key roles in alpha-synuclein-mediated apoptotic cell death. Cell death and differentiation 22, 2107-2122. 9. Mahul-Mellier, A.L., Fauvet, B., Gysbers, A., Dikiy, I., Oueslati, A., Georgeon, S., Lamontanara, A.J., Bisquertt, A., Eliezer, D., Masliah, E., et al. (2014). c-Abl phosphorylates alpha-synuclein and regulates its degradation: implication for alpha-synuclein clearance and contribution to the pathogenesis of Parkinson's disease. Human molecular genetics 23, 2858-2879. 10. Khalaf, O., Fauvet, B., Oueslati, A., Dikiy, I., Mahul-Mellier, A.L., Ruggeri, F.S., Mbefo, M.K., Vercruysse, F., Dietler, G., Lee, S.J., et al. (2014). The H50Q mutation enhances alpha-synuclein aggregation, secretion, and toxicity. The Journal of biological chemistry 289, 21856-21876. 11. Pazarentzos, E., Mahul-Mellier, A.L., Datler, C., Chaisaklert, W., Hwang, M.S., Kroon, J., Qize, D., Osborne, F., Al-Rubaish, A., Al-Ali, A., et al. (2014). IkappaBetaalpha inhibits apoptosis at the outer mitochondrial membrane independently of NF-kappaB retention. The EMBO journal 33, 2814-2828. 12. Datler, C., Pazarentzos, E., Mahul-Mellier, A.L., Chaisaklert, W., Hwang, M.S., Osborne, F., and Grimm, S. (2014). CKMT1 regulates the mitochondrial permeability transition pore in a process that provides evidence for alternative forms of the complex. Journal of cell science 127, 1816-1828. 13. Mahul-Mellier, A.L., Datler, C., Pazarentzos, E., Lin, B., Chaisaklert, W., Abuali, G., and Grimm, S. (2012). De-ubiquitinating proteases USP2a and USP2c cause apoptosis by stabilising RIP1. Biochimica et biophysica acta 1823, 1353-1365. 14. Mahul-Mellier, A.L., Pazarentzos, E., Datler, C., Iwasawa, R., AbuAli, G., Lin, B., and Grimm, S. (2012). De-ubiquitinating protease USP2a targets RIP1 and TRAF2 to mediate cell death by TNF. Cell death and differentiation 19, 891-899. 15. Iwasawa, R., Mahul-Mellier, A.L., Datler, C., Pazarentzos, E., and Grimm, S. (2011). Fis1 and Bap31 bridge the mitochondria-ER interface to establish a platform for apoptosis induction. The EMBO journal 30, 556-568. 16. Lemarie, A., Huc, L., Pazarentzos, E., Mahul-Mellier, A.L., and Grimm, S. (2011). Specific disintegration of complex II succinate:ubiquinone oxidoreductase links pH changes to oxidative stress for apoptosis induction. Cell death and differentiation 18, 338-349. 17. Irshad, S., Mahul-Mellier, A.L., Kassouf, N., Lemarie, A., and Grimm, S. (2009). Isolation of ORCTL3 in a novel genetic screen for tumor-specific apoptosis inducers. Cell death and differentiation 16, 890-898. 18. Mahul-Mellier, A.L., Strappazzon, F., Chatellard-Causse, C., Blot, B., Beal, D., Torch, S., Hemming, F., Petiot, A., Verna, J.M., Fraboulet, S., and Sadoul, R. (2009). Alix and ALG-2 make a link between endosomes and neuronal death. Biochemical Society transactions 37, 200-203. 19. Mahul-Mellier, A.L., Strappazzon, F., Petiot, A., Chatellard-Causse, C., Torch, S., Blot, B., Freeman, K., Kuhn, L., Garin, J., Verna, J.M., et al. (2008). Alix and ALG-2 are involved in tumor necrosis factor receptor 1-induced cell death. The Journal of biological chemistry 283, 34954-34965. 20. Mahul-Mellier, A.L., Hemming, F.J., Blot, B., Fraboulet, S., and Sadoul, R. (2006). Alix, making a link between apoptosis-linked gene-2, the endosomal sorting complexes required for transport, and neuronal death in vivo. The Journal of neuroscience : the official journal of the Society for Neuroscience 26, 542-549. 21. Fraboulet, S., Hemming, F.J., Mahul, A.L., Cristina, N., and Sadoul, R. (2003). Expression of Alix protein during early avian development. Gene expression patterns : GEP 3, 139-142.

EPFL SV BMI LMNN
AI 2149 (Bâtiment AI)
Station 19
CH-1015 Lausanne

Web site: Web site: https://lashuel-lab.epfl.ch/

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Administrative data

Fields of expertise

Developmental Biology: electroporation in vivo in chick embryo, handling and staging of early chick embryo, cryostat sectioning, histology, TUNEL staining, immunohistochemistry.

Molecular Biology:
Plasmid construction, site-directed mutagenesis (deletion, insertion, point mutation), TOPo-cloning; Gateway cloning; PCR, RT-PCR, Q-RT-PCR

Biochemical Biology:
Western Blot, bringing to light evidence of protein interactions through immunoprecipitation, ubiquitination/deubiquitination assay in vivo and in vitro, Kinase assay.

Cellular Biology:
cell culture, neuronal primary culture, transfection, siRNA, shRNA, stable clone, immunocytochemistry, immunofluorescence, confocal microscopy, flow cytometry, lentivirus production and infection.
Quantification of cell Death: TUNEL, Hoechst, Propidium iodide (PI), 3,3?-dihexyloxacarbocyanine iodide (DIOC), caspase activity and MTT-test.

Publications

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