Christian Depeursinge

Professeur honoraire

christian.depeursinge@epfl.ch http://apl.epfl.ch/muvision

EPFL PH-STI
AAB 2 01 (Bâtiment AAB)
Station 19
CH-1015 Lausanne

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Biography

Christian Depeursinge is the leader of the Microvision and Micro-Diagnostics (MVD) group at the Advanced Photonics Laboratory of the Institute of Microengineering at EPFL (Ecole Polytechnique Fédérale de Lausanne), Switzerland (http://apl.epfl.ch/muvision). His research and expertise in biomedical engineering and optics is internationally acknowledged. His current research topics include coherent and incoherent Imaging applied to diagnostics in biology, His research group pioneered in the development of DHM technology. He worked on several projects developed in cooperation with European and international partners. He is author and co-author of over 100 papers published in peer reviewed journals, several book chapters and more than 30 patents. He has given more than 20 invited lectures and plenaries in the last five years. He developed many projects in cooperation with national and international industries. He is co-founder of a start-up company (Lyncée Tec SA: www.Lynceetec.com). He is currently teaching at EPFL and occasionally in foreign universities and institutes.

ALUMNI

PhD Students Yves Delacrétaz (2009) Florian Charrière (2007) Tristan Colomb (2006) Anca Marian (2005) Frédéric Montfort (2005) Pia Massatsch (2003) Daniel Salzmann (2002) Philippe Thueler (2001) Laure Montandon-Varoda (2000) Etienne Cuche (2000) Frédéric Bevilacqua (1998) Laurent Thybaud (1997) Ramiro Conde (1995) Olivier Coquoz (1994) Julio Soruco (1993) Emmanuel B. de Haller (1993) Patrick Poscio (1992) Lionel Desponds (1991) Etienne Bornand (1986) Abdel Kader Boutaleb (1984) Yvan Pochon (1983) Master Students Christophe Pache (2008) Daniel Boss (2008) Mathieu Grossenbacher (2006) Nicolas Pavillon (2006) Yves Delacrétaz (2004) Florian Charrière (2003) Jonas Kühn (2003) Tristan Colomb (2000) Frédéric Montfort (2000) Pierre Marquet

RESEARCH INTERESTS

Optics plays an increasing role in nanotechnologies, life sciences, biophysical research, and biomedical engineering. Optical imaging, in particular, presents a renewal of interest, based on the development of new concepts in coherent imaging. Exploitation of light coherence appears as a cornerstone in the development of new imaging technology in microscopy and the analysis of mutually coherent fields generated by the sources of radiation (specially engineered or generated by the observed molecules themselves) will provide access to the intimate organization of biological matter, at the ultimate limit of single molecules. The development, in my research group, of digital holographic microscopy (DHM) appears as a generalization of optical coherence imaging approach, in that it collects the data simultaneously for many directions of the impinging optical beam and appears therefore as a unique tool to restore the pieces of information coherently delivered by the irradiated matter. Further on, the recourse to spectroscopy, more particularly to nonlinear optical properties of matter, biological matter in particular, will provide a unique access to the molecular structure and biochemical composition of living cells and tissues, thus offering probably one of the most attractive tools in the field of optical microscopy. The main research axis can be outlined as follows: 1) Tomography of cells and tissues 2) Super-resolution (nanometer range) 3) Extension of the coherent imaging technology to fluorescence imaging 4) Multi-modality imaging combining DHM and atomic force microscopy (AFM) 5) Functional imaging by coherent nonlinear microscopy 6) Imaging in turbid media

SELECTED RECENT PUBLICATIONS

For a full list of publications, please go here B. Rappaz, A. Barbul, Y. Emery, R. Korenstein, C. Depeursinge, P. J. Magistretti, P. Marquet, "Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy and impedance volume analyser", Cytometry A 73A, 895-903 (2008). J. Kühn, F. Charrière, T. Colomb, E. Cuche, F. Montfort, Y. Emery, P. Marquet, and C. Depeursinge, "Axial sub-nanometer accuracy in digital holographic microscopy", Measurement Science & Technology 19, 074007 (2008). B. Rappaz, F. Charrière, C. Depeursinge, P.J. Magistretti, and P. Marquet, "Simultaneous cell morphometry and refractive index measurement with dual-wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium", Opt. Lett. 33(7), 744-746 (2008). F. Charrière, A. Marian, T. Colomb, P. Marquet, and C. Depeursinge, "Amplitude point spread function measurement of high NA microscope objectives by digital holographic microscopy", Opt. Lett. 32(16), 2456-2458 (2007). F. Montfort, T. Colomb, F. Charrière, J. Kühn, P. Marquet, E. Cuche, S. Herminjard, and C. Depeursinge, "Submicrometer optical tomography by multiple-wavelength digital holographic microscopy", Appl. Opt. 45(32), 8209-8217 (2006). F. Charrière, N. Pavillon, T. Colomb, C. Depeursinge, T.J. Heger, E.A.D. Mitchell, P. Marquet, and B. Rappaz, "Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba", Opt. Express 14(16), 7005-7013 (2006). F. Charrière, A. Marian, F. Montfort, J. Kühn, T. Colomb, E. Cuche, P. Marquet, and C. Depeursinge, "Cell refractive index tomography by digital holographic microscopy", Opt. Lett. 31(2), 178-180 (2006). P. Marquet, B. Rappaz, P.J. Magistretti, E. Cuche, Y. Emery, T. Colomb, C. Depeursinge, "Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy", Opt. Lett. 30(5), 468-470 (2005). E. Cuche, P. Marquet, and C. Depeursinge, "Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms", Appl. Opt. 38(34), 6994-7001 (1999). E. Cuche, F. Bevilacqua, and C. Depeursinge, "Digital holography for quantitative phase-contrast imaging", Opt. Lett. 24(5), 291-293 (1999).

RECENT INVITED LECTURES

"Digital Holographic Microscopy (DHM): A versatile tool for micro- and nano- technology as well as biomedical applications", International Topical Meeting on Photonics, 2008, Hyogo, Japan. "Digital Holographic Microscopy: a new perspective in 3D imaging at the nanoscale", Biophotonics ASIA Optics Within Life Sciences (OWLS-10) conference, 2008, Singapore. (plenary) "Digital Holographic Microscopy (DHM) applied to metrology and biomedical applications", OFD'08 6th international conference on optics design and fabrication, 2008, Taipei, Taïwan. "Innovative aspects of Digital Holographic Microscopy", ELMI 8th European Light Microscopy Initiative, 2008, Davos, Switzerland. "Full numerical processing of optical data in microscopy: a perspective offered by digital holography", Photonics Ireland, 2007, Galway, Ireland. "Recent progress and perspectives in Digital Holographic Microscopy", OSA: Digital Holography and Three dimensional imaging, 2007, Vancouver, Canada. "Performance and limitations of Digital Holographic Microscopy: theory and practice", 3rd Asian and Pacific Rim Symposium on Biophotonics, 2007, Cairn, Australia. "DHM (Digital Holographic Microscopy): one step further towards full numerical optics in microscopy. Application to full tomography.", Workshop on Digital Holographic Reconstruction & Optical Tomography, 2007, Loughborough University, United Kingdom.

Publications

Publications Infoscience

Steer'n'Detect: fast 2D template detection with accurate orientation estimation

V. Uhlmann; Z. Puspoki; A. Depeursinge; M. Unser; D. Sage et al.

Bioinformatics. 2022-04-18. DOI : 10.1093/bioinformatics/btac270.

Christian Depeursinge

Professeur honoraire

Biography

ALUMNI

RESEARCH INTERESTS

SELECTED RECENT PUBLICATIONS

RECENT INVITED LECTURES

Publications

Publications Infoscience

Steer'n'Detect: fast 2D template detection with accurate orientation estimation

Principled Design and Implementation of Steerable Detectors

Texture-driven parametric snakes for semi-automatic image segmentation

Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence

Neural network training for cross-protocol radiomic feature standardization in computed tomography

Revealing Tumor Habitats from Texture Heterogeneity Analysis for Classification of Lung Cancer Malignancy and Aggressiveness

PET-based prognostic survival model after radiotherapy for head and neck cancer

The use of texture-based radiomics CT analysis to predict outcomes in early-stage non-small cell lung cancer treated with stereotactic ablative radiotherapy

Quantitative phase imaging in biomedicine

Rotation Invariance and Directional Sensitivity: Spherical Harmonics versus Radiomics Features

Holographic Visualisation and Interaction of Fused CT, PET and MRI Volumetric Medical Imaging Data Using Dedicated Remote GPGPU Ray Casting

Rotation-covariant tissue analysis for interstitial lung diseases using learned steerable filters: Performance evaluation and relevance for diagnostic aid

(F-18)-FDG PET/CT parameters to predict survival and recurrence in patients with locally advanced cervical cancer treated with chemoradiotherapy

Metabolic Tumor Volume and Total Lesion Glycolysis in Oropharyngeal Cancer Treated With Definitive Radiotherapy: Which Threshold Is the Best Predictor of Local Control?

3D Solid Texture Classification Using Locally-Oriented Wavelet Transforms

A PET-Based Nomogram for Oropharyngeal Cancers

Automated Classification of Brain Tumor Type in Whole-Slide Digital Pathology Images Using Local Representative Tiles

A 3-D Riesz-Covariance Texture Model for Prediction of Nodule Recurrence in Lung CT

Multidimensional Texture Analysis for Improved Prediction of Ultrasound Liver Tumor Response to Chemotherapy Treatment

Optimized Distributed Hyperparameter Search and Simulation for Lung Texture Classification in CT Using Hadoop

Overview of the Predictive Value of Quantitative 18 FDG PET in Head and Neck Cancer Treated with Chemoradiotherapy

Quantitative phase-digital holographic microscopy: a new imaging modality to identify original cellular biomarkers of diseases

Combining Unsupervised Feature Learning and Riesz Wavelets for Histopathology Image Representation: Application to Identifying Anaplastic Medulloblastoma

Towards an incoherent off-axis digital holographic microscope

Optimized Steerable Wavelets for Texture Analysis of Lung Tissue In 3-D CT: Classification of Usual Interstitial Pneumonia

Off-axis digital holographic camera for quantitative phase microscopy

Portable advanced off-axis camera for quantitative phase microscopy

Rotation-Covariant Texture Learning Using Steerable Riesz Wavelets

Three-dimensional solid texture analysis in biomedical imaging: Review and opportunities

Apparatus and method for quantitative phase tomography through linear scanning with coherent and non-coherent detection

A Practical Inverse-Problem Approach to Digital Holographic Reconstruction

Optical reconstruction of transparent objects with phase-only SLMs

Exploring Neural Cell Dynamics with Digital Holographic Microscopy

Quantitative phase noise in a two-color low coherence Digital Holographic Microscope

Full field vertical scanning in short coherence digital holographic microscope

Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy

Marker-free phase nanoscopy

3D Optical Imaging of Living Cells in Microgravity

Label-Free Cytotoxicity Screening Assay by Digital Holographic Microscopy

Simultaneous Optical Recording in Multiple Cells by Digital Holographic Microscopy of Chloride Current Associated to Activation of the Ligand-Gated Chloride Channel GABA(A) Receptor

Spatially-Resolved Eigenmode Decomposition of Red Blood Cells Membrane Fluctuations Questions the Role of ATP in Flickering

Local demodulation of holograms using the Riesz transform with application to microscopy

Accelerated autofocusing of off-axis holograms using critical sampling

Near-Affine-Invariant Texture Learning for Lung Tissue Analysis Using Isotropic Wavelet Frames

Quantitative Measurement of absolute cell volume and intracellular integral refractive index (RI) with Dual-wavelength Digital Holographic Microscopy (DHM)

Digital holographic microscopy for the cytomorphological imaging of cells under zero gravity

Single shot dual wavelength full field imaging in low coherence digital holographic microscopy

Early Cell Death Detection with Digital Holographic Microscopy

Monte Carlo simulation of the field back-scattered from rough surfaces

Reconstruction in interferometric synthetic aperture microscopy: comparison with optical coherence tomography and digital holographic microscopy

Enhanced robustness digital holographic microscopy for demanding environment of space biology

Resolution and Complex Deconvolution in Holographic Microscopy

Holographic second harmonic generation imaging

Single-shot, simultaneous incoherent and holographic microscopy

Complex index refraction tomography with sub ?/6-resolution

Dual wavelength full field imaging in low coherence digital holographic microscopy

Beyond the lateral resolution limit by phase imaging

Realistic 3D coherent transfer function inverse filtering of complex fields

Determination of Transmembrane Water Fluxes in Neurons Elicited by Glutamate Ionotropic Receptors and by the Co-transporters KCC2 and NKCC1: A Digital Holographic Microscopy Study

Quantitative phase restoration by direct inversion using the optical transfer function

Holographic Second Harmonic Generation Microscopy

Dual-wavelength Digital Holography for quantification of cell volume and integral refractive index (RI)

Cell death detection and ionic homeostasis monitoring with digital holographic microscopy

Holographic Second Harmonic Generation Imaging

Three-dimensional deconvolution of complex fields

Cell Death and Ionic Regulation Detection with Digital Holographic Microscopy

Cellular Dynamics and Three-Dimensional Refractive Index Distribution Studied with Quantitative Phase Imaging

Holographic second harmonic generation (SHG) microscopy

Complex field imaging for diffraction tomography

Quantitative phase from defocus

Holographic Second Harmonic Generation Microscopy