Single-Metabolite Bio-Nano-Sensors and System for Remote Monitoring in Animal Models
A novel system for remote monitoring of metabolism in animal model is proposed in this paper. The system is obtained by integrating Bio-Nano-Sensors to detect single- metabolites, an electrochemical front-end made with off- the-shelf components, an RF communication sub-system, and an antenna of new design. The system has been calibrated and tested for continuous monitoring of four different metabolites: glucose, lactate, glutamate, and adenosine triphosphate (ATP). Tests with animal models (mice) have been conducted to investigate tissue inflammation induced by the implanted Bio-Nano- Sensors. The tests confirmed that our system is suitable and reliable for remote monitoring of single-metabolites in experiments with animal models.2011. IEEE Sensors 2011 Conference, Limerick, Ireland, October 28-31, 2011. p. 716-719.
Full text: http://infoscience.epfl.ch/record/169913/files/IEEE_Sensors_2011_L%C3%A9andre_Bolomey_system.pdf
Instrumented Prosthesis for Knee Implants Monitoring
In this work we present an instrumented smart knee prosthesis for in-vivo measurement of forces and kinematics. Studying the constraints, we designed minimal sensory systems to be placed in the polyethylene part of the prosthesis. The magnetic sensors and a permanent magnet are chosen and configured to measure the relative kinematics of the prosthesis. Moreover, the strain gauges were designed to measure the forces on the polyethylene part. The kinematic and kinetic measurements on a mechanical knee simulator are validated toward reference systems. The supplementary electronics, including the A/D, amplifier, rectifier and voltage doubler are designed. Consequently, by considering the necessary power budget for all the components to be performed, the optimal coils for remote powering is investigated. The system will be packaged in the polyethylene part. Therefore, by the end we will have a smart polyethylene part which can be easily modified for different types of the knee prosthesis without changing the prosthesis design.2011. IEEE Conference on Automation Science and Engineering (IEEE CASE), Trieste, Italy, August 24 - 27, 2011. p. 828-835. DOI : 10.1109/CASE.2011.6042490.
MULTI-ANALYTE DETECTION FOR BIOLOGICAL FLUIDS Towards Continous Monitoring of Glucose, Ionized Calcium and pH using a Viscometric Affinity Biosensor
We present a viscometric affinity biosensor that can potentially allow continuous multi-analyte monitoring in biological fluids like blood or plasma. The sensing principle is based on the detection of viscosity changes of a polymeric solution which has a selective affinity for the analyte of interest. The chemico-mechanical sensor incorporates an actuating piezoelectric diaphragm, a sensing piezoelectric diaphragm and a flow-resisting microchannel for viscosity detection. A free-standing Anodic Alumina Oxide (AAO) porous nano-membrane is used as selective interface. A glucose-sensitive sensor was fabricated and extensively assessed in buffer solution. The sensor reversibility, stability and sensitivity were excellent during at least 65 hours. Results showed also a good degree of stability for a long term measurement (25 days). The sensor behaviour was furthermore tested in fetal bovine serum (FBS). The obtained results for glucose sensing are very promising, indicating that the developed sensor is a candidate for continuous monitoring in biological fluids. Sensitive solutions for ionized calcium and pH are currently under development and should allow multi-analyte sensing in the near future.2011. International Conference on Biomedical Electronics and Devices , Rome, Italy, 26-29 January, 2011 . p. 295-298. DOI : 10.5220/0003350902950298.
Full text: http://infoscience.epfl.ch/record/168190/files/PaperBiodevice2011.pdf
Compression Schemes for In-body and On-body UWB Sensor Networks2010. ISABEL, Rome, Italy, November, 2010.
Full text: http://infoscience.epfl.ch/record/161619/files/isabel2010_submission_187.pdf
In-Vitro Platform to study Ultrasound as Source for Wireless Energy Transfer and Communication for Implanted Medical Devices
A platform to study ultrasound as a source for wireless energy transfer and communication for implanted medical devices is described. A tank is used as a container for a pair of electroacoustic transducers, where a control unit is fixed to one wall of the tank and a transponder can be manually moved in three axes and rotate using a mechanical system. The tank is filled with water to allow acoustic energy and data transfer, and the system is optimized to avoid parasitic effects due to cables, reflection paths and cross talk problems. A printed circuit board is developed to test energy scavenging such that enough acoustic intensity is generated by the control unit to recharge a battery loaded to the transponder. In the same manner, a second printed circuit board is fabricated to study transmission of information through acoustic waves.2010. 32nd Annual International Conference of the IEEE EMBS, Buenos Aires. p. 3751-3754.
Full text: http://infoscience.epfl.ch/record/154838/files/In-Vitro%20Platform%20to%20study%20Ultrasound%20as%20Source%20for%20Wireless%20Energy%20Transfer%20and%20Communication%20for%20Implanted%20Medical%20Devices.pdf
Size-Selective Diffusion in Nanoporous but Flexible Membranes for Glucose Sensors
A series of nanoporous membranes prepared from polyethylene-block-polystyrene were applied for size-selective diffusion of glucose and albumin molecules. Millimeter-sized test cells for characterization of such molecular diffusions were designed assuming an implantable glucose sensor. The prepared nanoporous membrane exhibits excellent flexibility and toughness compared to conventional nanoporous membranes of brittle alumina. Pore size of the membranes could be controlled from 5 to 30 nm by varying preparation conditions. All of these nanoporous membranes prepared in this study let glucose pass through, indicating a continuous pore connection through the entire thickness of the membrane in a few tens of micrometers. In contrast, membranes prepared under optimum conditions could perfectly block albumin permeation. This means that these vital molecules having different sizes can be selectively diffused through the nanoporous membranes. Such a successful combination of size selectivity of molecular diffusion in nanoscale and superior mechanical properties in macroscale is also beneficial for other devices requesting down-sized manufacture.Acs Nano. 2009. DOI : 10.1021/nn8008728.
Novel chemico-mechanical approach towards long-term implantable glucose sensing
The proof of concept of a continuously sensing affinity device based on the glucose-dependent viscosity of a sensitive solution containing dextran and Concanavalin A has been successfully demonstrated. The biosensor incorporates a piezoelectric diaphragm and a flow-resisting microchannel for viscosity detection, and a free-standing Anodic Alumina Oxide (AAO) porous nano-membrane as glucose selective interface. Extensive in vitro glucose measurements between two physiologically relevant glucose concentrations, 2 mM and 9 mM (respectively hypo- and hyperglycemia), were successfully performed during 4 days. To the best of our knowledge, such reversibility and stability of glucose measurement over time had not been reported yet.2009. Eurosensors XXIII, Lausanne, Switzerland, September 7-9, 2009. p. 313-316. DOI : 10.1016/j.proche.2009.07.078.
Full text: http://infoscience.epfl.ch/record/141381/files/2009-Boss-Eurosensors.pdf