Fields of expertise
Expert in production and Purification of macromolecules, such as antibodies, membrane proteins and growth factors.
MissionThe mission of the facility is to connect researchers in Protein Sciences and Integrative Structural Biology. We advice, support and train scientists in production, purification and biophysical/structural characterisations of macromolecules, such as enzymes, antibodies or any kind of proteins you are interested in.
Current workDaily support of scientists in X-ray crystallography, Bio-NMR and Single particle cryoEM pipelines to obtain atomic structures of their favourite proteins
Daily support of scientists in production and purification of proteins in various hosts
Daily support of scientists in biophysical techniques and protein quality control
Head of Protein Production and Structure Core Facility
School of Life Sciences
Chair of Microbial Pathogenesis, Prof. Stewart Cole
Structural Biology lab, Prof. Joseph Noel
The Salk Institute (La Jolla, USA)
Machine learning predictions of MHC-II specificities reveal alternative binding mode of class II epitopesImmunity. 2023-06-13. DOI : 10.1016/j.immuni.2023.03.009.
Uncovering and engineering the mechanical properties of the adhesion GPCR ADGRG1 GAIN domain2023-04-06. DOI : 10.1101/2023.04.05.535724.
Broadly potent anti-SARS-CoV-2 antibody shares 93% of epitope with ACE2 and provides full protection in monkeysJournal of Infection. 2023. DOI : 10.1016/j.jinf.2023.10.008.
Structure-based optimization of type III indoleamine 2,3-dioxygenase 1 (IDO1) inhibitorsJournal Of Enzyme Inhibition And Medicinal Chemistry. 2022-12-31. DOI : 10.1080/14756366.2022.2089665.
Palmitoylated acyl protein thioesterase APT2 deforms membranes to extract substrate acyl chainsNature Chemical Biology. 2021-04-01. DOI : 10.1038/s41589-021-00753-2.
Azole-Based Indoleamine 2,3-Dioxygenase 1 (IDO1) InhibitorsJournal Of Medicinal Chemistry. 2021-02-25. DOI : 10.1021/acs.jmedchem.0c01968.
Inhibition Mechanisms of Indoleamine 2,3-Dioxygenase 1 (IDO1)Journal Of Medicinal Chemistry. 2019-10-10. DOI : 10.1021/acs.jmedchem.9b00942.
Discovery of benzothiazoles as antimycobacterial agents: Synthesis, structure-activity relationships and binding studies with Mycobacterium tuberculosis decaprenylphosphoryl-beta-D-ribose 2 '-oxidaseBioorganic & Medicinal Chemistry. 2015. DOI : 10.1016/j.bmc.2015.11.017.
Structure of EspB, a secreted substrate of the ESX-1 secretion system of Mycobacterium tuberculosisJournal Of Structural Biology. 2015. DOI : 10.1016/j.jsb.2015.06.003.
2-Carboxyquinoxalines Kill Mycobacterium tuberculosis through Noncovalent Inhibition of DprE1Acs Chemical Biology. 2015. DOI : 10.1021/cb5007163.
Dithiol amino acids can structurally shape and enhance the ligand-binding properties of polypeptidesNature Chemistry. 2014. DOI : 10.1038/nchem.2043.
The cysteine desulfurase IscS of Mycobacterium tuberculosis is involved in iron sulfur cluster biogenesis and oxidative stress defenceBiochemical Journal. 2014. DOI : 10.1042/Bj20130732.
Functional Dissection of Intersubunit Interactions in the EspR Virulence Regulator of Mycobacterium tuberculosisJournal Of Bacteriology. 2014. DOI : 10.1128/Jb.00039-1.
Pyridomycin bridges the NADH- and substratebinding pockets of the enoyl reductase InhANature Chemical Biology. 2014. DOI : 10.1038/nchembio.1405.
Phenotypic Profiling of Mycobacterium tuberculosis EspA Point Mutants Reveals that Blockage of ESAT-6 and CFP-10 Secretion In Vitro Does Not Always Correlate with Attenuation of VirulenceJournal Of Bacteriology. 2013. DOI : 10.1128/Jb.00967-13.
Mycobacterium tuberculosis EspB binds phospholipids and mediates EsxA-independent virulenceMolecular Microbiology. 2013. DOI : 10.1111/mmi.12336.
Structural Basis for Benzothiazinone-Mediated Killing of Mycobacterium tuberculosisScience Translational Medicine. 2012. DOI : 10.1126/scitranslmed.3004395.
Virulence Regulator EspR of Mycobacterium tuberculosis Is a Nucleoid-Associated ProteinPlos Pathogens. 2012. DOI : 10.1371/journal.ppat.1002621.
Structure and function of the transketolase from Mycobacterium tuberculosis and comparison with the human enzymeOpen Biology. 2012. DOI : 10.1098/rsob.110026.
EspD Is Critical for the Virulence-Mediating ESX-1 Secretion System in Mycobacterium tuberculosisJournal of bacteriology. 2011. DOI : 10.1128/JB.06417-11.
Atypical DNA recognition mechanism used by the EspR virulence regulator of Mycobacterium tuberculosisMolecular Microbiology. 2011. DOI : 10.1111/j.1365-2958.2011.07813.x.
Towards anti-virulence drugs targeting ESX-1 mediated pathogenesis of Mycobacterium tuberculosisDrug Discovery Today: Disease Mechanisms. 2010. DOI : 10.1016/j.ddmec.2010.09.002.
Sigma Factor F does not Prevent Rifampin Inhibition of RNA Polymerase or Cause Rifampin Tolerance in Mycobacterium tuberculosisJournal of bacteriology. 2010. DOI : 10.1128/JB.00687-10.
Teaching & PhD
Life Sciences Engineering