Florian Maria Wurm
Retirement
Professor Wurm retired in March 2015. His laboratory has been closed with no successor. Wurm continues to be active - scientifically and otherwise and can be approached for questions and issues in pharmaceutical biotechnology with emphasis on recombinant protein and biological generation for pharmaceutical use. He is also active within one of the companies he has founded - most notably ExcellGene SA, in Monthey, Wallis (CH). He has published more than 300 papers and has an H-index of 65.Current work
Wurm continues to be active scientifically and in other functions, most notably within his company ExcellGene SA in Monthey. As Chief Scientific Officer, co-owner, President of the Board he pursues, with the help of the Director of Research Dr. Paco Pino, relevant topics in manufacturing sciences, with the emphasis of high level production of biologics (AAV) and of therapeutic and prophylactic proteins from animal cells in bioreactors.Biography
Florian Wurm received his academic training as a Biologist and Molecular Geneticist at the University of Giessen. He joined the Hoechst AG (Behringwerke) in Marburg as head of a laboratory in Virology.Working with immortalized mammalian cells for the establishment of production processes for alpha-interferons provided the first opportunity to combine basic research with medical application. In 1984 he joined Harvard Medical School in Boston as a Research Fellow in Molecular Biology. 1986 he took an offer from Genentech Inc. in San Francisco to work in Process Sciences on the development of large scale manufacturing processes for recombinant proteins. There he has held a number of leading positions and has acquired intimate knowledge in the generation of protein pharmaceuticals in mammalian cells in bioreactors (a number of which are now marketed products).
In 1995 he joined the EPFL as a Professor for Biotechnology. Wurm has published more than 300 scientific papers and holds more than 20 patents/patent-applications. His work is cited more than 19'000 times. His H-index stands at 65 in 2024.
He was Chairman (2005-2009) and is member of the Executive Board of the European Society of Animal Cell Technology (ESACT). He serves as a consultant to the pharmaceutical Biotech Industry, mainly in the fields of animal cell technology for recombinant protein production and in regulatory affairs. He works as a scientific reviewer and editior/asscciate editor for a number of international journals in the Biotech field. F.M. Wurm teaches classes to pre- and postgraduate students in the fields of Molecular and Cellular Biotechnology.
He was founder and Chief Scientific Officer of ExcellGene SA, a 2001 established company in Monthey, Switzerland. He took the position of President and CEO of ExcellGene in 2015. He retired from the CEO position in 2017 and continues to be President and Chief Scientific Officer of ExcellGene.
In 2008 Dr. Wurm was appointed Visiting Professor for Biotechnology at Jinan University in Guangzhou, China.
He retired from his position at the EPFL in 2015. His laboratory is closed.
With his team at ExcellGene and in collaboration with Dr. Paco Pino, Director of R&D, he continues to explore manufacturing sciences with animal cells in bioreactors.
Publications
Infoscience publications
All publications
* High-Titer Neutralizing Antibodies against the SARS-CoV-2 Delta Variant Induced by Alhydroxyquim-II-Adjuvanted Trimeric Spike Antigens
Microbiology Spectrum. 2022-02-01. DOI : 10.1128/spectrum.01695-21.* Fluid dynamics of a pilot-scale OrbShake bioreactor under different operating conditions
Journal Of Chemical Technology And Biotechnology. 2022. DOI : 10.1002/jctb.6995.* Boosted Pro-Inflammatory Activity in Human PBMCs by Lipopolysaccharide and SARS-CoV-2 Spike Protein Is Regulated by alpha-1 Antitrypsin
International Journal Of Molecular Sciences. 2021-08-01. DOI : 10.3390/ijms22157941.* Considerations of the Impacts of Cell-Specific Growth and Production Rate on Clone Selection-A Simulation Study
Processes. 2021-06-01. DOI : 10.3390/pr9060964.* Naming CHO cells for bio-manufacturing: Genome plasticity and variant phenotypes of cell populations in bioreactors question the relevance of old names
Biotechnology Journal. 2021-06-25. DOI : 10.1002/biot.202100165.* Asymmetric opening of the homopentameric 5-HT3A serotonin receptor in lipid bilayers
Nature Communications. 2021-02-16. DOI : 10.1038/s41467-021-21016-7.* Trimeric SARS-CoV-2 Spike Proteins Produced from CHO Cells in Bioreactors Are High-Quality Antigens
Processes. 2020-12-01. DOI : 10.3390/pr8121539.* Designs and Characterization of Subunit Ebola GP Vaccine Candidates: Implications for Immunogenicity
Frontiers In Immunology. 2020-11-04. DOI : 10.3389/fimmu.2020.586595.* Analysis of volumetric mass transfer coefficient (k(L)a) in small- (250 mL) to large-scale (2500 L) orbitally shaken bioreactors
3 Biotech. 2020-08-19. DOI : 10.1007/s13205-020-02352-9.* The Delivery of alpha 1-Antitrypsin Therapy Through Transepidermal Route: Worthwhile to Explore
Frontiers In Pharmacology. 2020-07-03. DOI : 10.3389/fphar.2020.00983.* Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells
Molecular Therapy-Methods & Clinical Development. 2019-06-14. DOI : 10.1016/j.omtm.2018.11.004.* Recombinant Proteins for Industrial versus Pharmaceutical Purposes: A Review of Process and Pricing
Processes. 2019-08-01. DOI : 10.3390/pr7080476.* Contactless Respiration Monitoring in Real-Time via a Video Camera
2018-01-01. Joint Conference of the European Medical and Biological Engineering Conference (EMBEC) / Nordic-Baltic Conference on Biomedical Engineering and Medical Physics (NBC), Tampere, FINLAND, Jun, 2017. p. 567-570. DOI : 10.1007/978-981-10-5122-7_142.* Improved process conditions for increasing expression of MHC class II protein from a stable Drosophila S2 cell line
BIOTECHNOLOGY LETTERS. 2018. DOI : 10.1007/s10529-017-2440-0.* Fluid dynamics of flow fields in a disposable 600-mL orbitally shaken bioreactor
Biochemical Engineering Journal. 2018. DOI : 10.1016/j.bej.2017.10.019.* Cloning of CHO Cells, Productivity and Genetic Stability—A Discussion
Processes. 2017-04-20. DOI : 10.3390/pr5020020.* Validation of Scalable and Compliant AAV Production
2017. 20th Annual Meeting of the American-Society-of-Gene-and-Cell-Therapy (ASGCT), Washington, DC, MAY 10-13, 2017. p. 326-326.* Studies on fluid dynamics of the flow field and gas transfer in orbitally shaken tubes
Biotechnology Progress. 2017. DOI : 10.1002/btpr.2375.* Multigene expression in stable CHO cell pools generated with the piggyBac transposon system
Biotechnology Progress. 2016. DOI : 10.1002/btpr.2319.* A comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation
Biotechnology Progress. 2016. DOI : 10.1002/btpr.2328.* Recombinant protein production from stable mammalian cell lines and pools
Current Opinion In Structural Biology. 2016. DOI : 10.1016/j.sbi.2016.06.005.* Suspension-Adapted HEK 293 Cells in Orbital Shaken Bioreactors for the Production of Adeno-Associated Virus Vectors
2016. 19th Annual Meeting of the American-Society-of-Gene-and-Cell-Therapy (ASGCT), Washington DC, USA, May 4-7, 2016. p. S183-S184.* Comparison of Three Transposons for the Generation of Highly Productive Recombinant CHO Cell Pools and Cell Lines
Biotechnology And Bioengineering. 2016. DOI : 10.1002/bit.25888.* Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension
Analytical Biochemistry. 2016. DOI : 10.1016/j.ab.2016.04.009.* Enhanced Plasmid DNA Utilization in Transiently Transfected CHO-DG44 Cells in the Presence of Polar Solvents
Biotechnology Progress. 2015. DOI : 10.1002/btpr.2152.* A simple plasmid-based transient gene expression method using High Five cells
Journal Of Biotechnology. 2015. DOI : 10.1016/j.jbiotec.2015.10.007.* Production of active glycosylation-deficient gamma-secretase complex for crystallization studies
Biotechnology And Bioengineering. 2015. DOI : 10.1002/bit.25675.* Transient Production of Recombinant Adeno-Associated Virus (AAV) Vectors for Gene Therapy Applications Using Suspension-Adapted HEK 293 Cells in Orbital Shaken Bioreactors
2015. 18th Annual Meeting of the American-Society-of-Gene-and-Cell-Therapy (ASGCT)'. p. S184-S185.* Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools
Journal of Biotechnology. 2015. DOI : 10.1016/j.jbiotec.2015.03.001.* Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells
Biotechnology Progress. 2015. DOI : 10.1002/btpr.2064.* Study of transposon-mediated cell pool and cell line generation in CHO cells
Lausanne, EPFL, 2015. DOI : 10.5075/epfl-thesis-6563.* Development of plasmid-based expression technology for rapid and sustained production of recombinant proteins in insect cells
Lausanne, EPFL, 2014. DOI : 10.5075/epfl-thesis-6435.* Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells
International journal of molecular and cellular medicine. 2014.* The Engineering of a Scalable Disposable Orbitally Shaken Bioreactor System for Animal Cell Cultivation and Therapeutic Protein Production
Lausanne, EPFL, 2014. DOI : 10.5075/epfl-thesis-6423.* Surface wave dynamics in orbital shaken cylindrical containers
Physics of Fluids. 2014. DOI : 10.1063/1.4874612.* Virus-free transient protein production in Sf9 cells
Journal Of Biotechnology. 2014. DOI : 10.1016/j.jbiotec.2013.11.018.* CHO Quasispecies—Implications for Manufacturing Processes
Processes. 2013. DOI : 10.3390/pr1030296.* The optimization of a rapid low-cost alternative of large-scale medium sterilization
2013. 23rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health, Lille, France, 23-26 June 2013. p. P45. DOI : 10.1186/1753-6561-7-S6-P45.* Study of the improved Sf9 transient gene expression process
2013. 23rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health, Lille, France, 23-26 June 2013. p. P19. DOI : 10.1186/1753-6561-7-S6-P19.* Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells
Protein Expression and Purification. 2013. DOI : 10.1016/j.pep.2013.09.001.* High expression of the aspartate–glutamate carrier Aralar1 favors lactate consumption in CHO cell culture
Pharmaceutical Bioprocessing. 2013. DOI : 10.4155/pbp.13.5.* Disposable 600-mL orbitally shaken bioreactor for mammalian cell cultivation in suspension
Biochemical Engineering Journal. 2013. DOI : 10.1016/j.bej.2013.04.008.* Numerical simulation of orbitally shaken viscous fluids with free surface
International Journal For Numerical Methods In Fluids. 2013. DOI : 10.1002/fld.3658.* Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures
Processes. 2013. DOI : 10.3390/pr1010003.* DNA delivery with hyperbranched polylysine: A comparative study with linear and dendritic polylysine
Journal of Controlled Release. 2013. DOI : 10.1016/j.jconrel.2013.01.019.* Highly efficient production of the Alzheimer's γ-Secretase integral membrane protease complex by a multi-gene stable integration approach
Biotechnology and Bioengineering. 2013. DOI : 10.1002/bit.24851.* A Simple High Yielding Transient Gene Expression System for CHO Cells
Lausanne, EPFL, 2013. DOI : 10.5075/epfl-thesis-5572.* The Use of Valproic Acid in Transient Gene Expression with HEK-293E Cells
Lausanne, EPFL, 2013. DOI : 10.5075/epfl-thesis-5498.* Lactate metabolism shift in CHO cell culture: the role of mitochondrial oxidative activity
New Biotechnology. 2013. DOI : 10.1016/j.nbt.2012.05.021.* Comparative Study on the In Vitro Cytotoxicity of Linear, Dendritic, and Hyperbranched Polylysine Analogues
Biomacromolecules. 2012. DOI : 10.1021/bm300930j.* A NanoDrop-based method for rapid determination of viability decline in suspension cultures of animal cells
Analytical Biochemistry. 2012. DOI : 10.1016/j.ab.2012.08.028.* Multidisciplinary Analysis of the Metabolic Shift to Lactate Consumption in CHO Cell Culture
Lausanne, EPFL, 2012. DOI : 10.5075/epfl-thesis-5420.* Power input correlation to characterize the hydrodynamics of cylindrical orbitally shaken bioreactors
Biochemical Engineering Journal. 2012. DOI : 10.1016/j.bej.2012.04.007.* Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells
Biotechnology and Bioengineering. 2012. DOI : 10.1002/bit.24494.* Poly(ethyleneimine)-Mediated Large-Scale Transient Gene Expression: Influence of Molecular Weight, Polydispersity and N-Propionyl Groups
Macromolecular Bioscience. 2012. DOI : 10.1002/mabi.201100404.* k(L)a as a predictor for successful probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors
New biotechnology. 2012. DOI : 10.1016/j.nbt.2011.10.010.* Reduced glutamine concentration improves protein production in growth-arrested CHO-DG44 and HEK-293E cells
Biotechnology letters. 2012. DOI : 10.1007/s10529-011-0809-z.* Flexible antibodies with nonprotein hinges
Proceedings Of The Japan Academy Series B-Physical And Biological Sciences. 2011. DOI : 10.2183/pjab.87.603.* Hydrodynamic stress in orbitally shaken bioreactors
2011. p. P39. DOI : 10.1186/1753-6561-5-S8-P39.* Transient gene expression with CHO cells in conditioned medium: a study using TubeSpin® bioreactors
2011. p. P38. DOI : 10.1186/1753-6561-5-S8-P38.* Transient transfection of insect Sf-9 cells in TubeSpin® bioreactor 50 tubes
2011. p. P37. DOI : 10.1186/1753-6561-5-S8-P37.* Influence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells
2011. p. P35. DOI : 10.1186/1753-6561-5-S8-P35.* Rapid recombinant protein production from pools of transposon-generated CHO cells
2011. p. P34. DOI : 10.1186/1753-6561-5-S8-P34.* The use of filler DNA for improved transfection and reduced DNA needs in transient gene expression with CHO and HEK cells
2011. p. P33. DOI : 10.1186/1753-6561-5-S8-P33.* Transposon mediated co-integration and co-expression of transgenes in CHO-DG44 cells
2011. p. P32. DOI : 10.1186/1753-6561-5-S8-P32.* CHO cell lines generated by PiggyBac transposition
2011. p. P31. DOI : 10.1186/1753-6561-5-S8-P31.* Structural analysis of intact monoclonal antibodies by electron transfer dissociation mass spectrometry
Analytical chemistry. 2011. DOI : 10.1021/ac201293m.* Mobile phone-induced honeybee worker piping
Apidologie. 2011. DOI : 10.1007/s13592-011-0016-x.* Large-Scale Transfection of Mammalian Cells
Protein Expression in Mammalian Cells; Humana Press, 2011. p. 13-26.* First CHO genome
Nature Biotechnology. 2011. DOI : 10.1038/nbt.1943.* The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability
Biotechnology and Bioengineering. 2011. DOI : 10.1002/bit.23167.* A simple high-yielding process for transient gene expression in CHO cells
Journal of Biotechnology. 2011. DOI : 10.1016/j.jbiotec.2011.03.001.* OrbShake Bioreactors for Mammalian Cell Cultures
Lausanne, EPFL, 2011. DOI : 10.5075/epfl-thesis-5135.* Efficient and reproducible mammalian cell bioprocesses without probes and controllers?
New biotechnology. 2011. DOI : 10.1016/j.nbt.2011.02.004.* Manufacturing recombinant proteins in kg-ton quantities using animal cells in bioreactors
European Journal of Pharmaceutics and Biopharmaceutics. 2011. DOI : 10.1016/j.ejpb.2011.01.005.* TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension
Biotechnology Letters. 2011. DOI : 10.1007/s10529-011-0527-6.* Role of polymer architecture on polycation induced cell death: systematic study on molecular mechanism
2010. 3rd International Cellular Delivery of Therapeutic Macromolecules (CDTM) Symposium, Cardiff, ENGLAND, Jun 26-29, 2010. p. 1086-1086. DOI : 10.1016/j.drudis.2010.09.369.* Innovative, Non-stirred Bioreactors in Scales from Milliliters up to 1000 Liters for Suspension Cultures of Cells using Disposable Bags and Containers – A Swiss Contribution
CHIMIA International Journal for Chemistry. 2010. DOI : 10.2533/chimia.2010.819.* Generation of stable, high-producing cho cell lines by lentiviral vector-mediated gene transfer in serum-free suspension culture
Biotechnology and Bioengineering. 2010. DOI : 10.1002/bit.22968.* Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells
Journal of biotechnology. 2010. DOI : 10.1016/j.jbiotec.2010.05.003.* Lentiviral Vector-Mediated Gene Delivery for the Generation of Chinese Hamster Ovary Cell Lines with Improved Productivity and Stability
Lausanne, EPFL, 2010. DOI : 10.5075/epfl-thesis-4834.* Hyperosmolarity enhances transient recombinant protein yield in Chinese hamster ovary cells
Biotechnology Letters. 2010. DOI : 10.1007/s10529-010-0331-8.* Transient Gene Expression in HEK-293E Cells for Recombinant Protein Production
Lausanne, EPFL, 2010. DOI : 10.5075/epfl-thesis-4815.* Determination of a scale-up factor from mixing time studies in orbitally shaken bioreactors
Biochemical Engineering Journal. 2010. DOI : 10.1016/j.bej.2010.08.005.* Compressed collagen gel: a novel scaffold for human bladder cells
Journal of Tissue Engineering and Regenerative Medicine. 2010. DOI : 10.1002/term.222.* Isolation and evaluation of novel adeno-associated virus sequences from porcine tissues
Gene Therapy. 2009. DOI : 10.1038/gt.2009.82.* Transient Gene Expression in Mammalian Cells: Promises and Challenges for Medical Biotechnology
Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology; Hoboken, N.J: Wiley, 2009.* Orbitally shaken bioreactors for mammalian cell culture
Lausanne, EPFL, 2009. DOI : 10.5075/epfl-thesis-4492.* DNA delivery by microinjection for the generation of recombinant mammalian cell lines
Microinjection: Methods and Applications; Humana Press, 2009. p. 99-112.* 25 years of recombinant proteins from reactor-grown cells — Where do we go from here?
Biotechnology Advances. 2009. DOI : 10.1016/j.biotechadv.2009.05.008.* Use of Orbital Shaken Disposable Bioreactors for Mammalian Cell Cultures from the Milliliter-Scale to the 1,000-Liter Scale
Advances in Biochemical Engineering / Biotechnology. 2009. DOI : 10.1007/10_2008_18.* Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells
Vaccine. 2009. DOI : 10.1016/j.vaccine.2009.06.019.* Studies on scaffold design for tissue engineering applications
Lausanne, EPFL, 2009. DOI : 10.5075/epfl-thesis-4445.* Suspension-adapted Chinese hamster ovary-derived cells expressing green fluorescent protein as a screening tool for biomaterials
Biotechnol Lett. 2009. DOI : 10.1007/s10529-009-9997-1.* Transient recombinant protein expression in mammalian cells
Lausanne, EPFL, 2009. DOI : 10.5075/epfl-thesis-4393.* Production and characterisation of rationally optimised, anti-melanoma T-cell receptors: a step closer towards immunotherapeutic treatment for patients
2008. Annual joint meeting of the Swiss Societies for Pneumology, Paediatric Pneumology, Allergology and Immunology, Thoracic Surgery, Fribourg, April 17-18, 2008. p. 44S.* Recombinant human collagen as a bioactive element in scaffolds for tissue engineering
Annual Tissue-Engineering-and-Regenerative-Medicine-International-Society-European-Chapter Meeting, Porto, PORTUGAL, Jun, 2008.* Compressed collagen-PLA hybrids as scaffold for human bladder cells
Annual Tissue-Engineering-and-Regenerative-Medicine-International-Society-European-Chapter Meeting, Porto, PORTUGAL, Jun, 2008.* The kinetics of polyethylenimine-mediated transfection in suspension cultures of Chinese hamster ovary cells
Molecular Biotechnology. 2008. DOI : 10.1007/s12033-008-9069-0.* Recombinant therapeutic protein production in cultivated mammalian cells: current status and future prospects
Drug Discovery Today: Technologies. 2008. DOI : 10.1016/j.ddtec.2008.12.003.* Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells
Biotechnology Progress. 2008. DOI : 10.1021/bp070286c.* Valproic acid: A viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures
Biotechnology and Bioengineering. 2008. DOI : 10.1002/bit.21882.* Shaken helical track bioreactors: Providing oxygen to high-density cultures of mammalian cells at volumes up to 1000L by surface aeration with air
New Biotechnology. 2008. DOI : 10.1016/j.nbt.2008.03.001.* Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions
Nucleic Acids Research. 2008. DOI : 10.1093/nar/gkn423.* Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection
Biotechnology and Bioengineering. 2008. DOI : 10.1002/bit.21972.* Coexpression of acidic fibroblast growth factor enhances specific productivity and antibody titers in transiently transfected HEK293 cells
New Biotechnology. 2008. DOI : 10.1016/j.nbt.2008.08.007.* Development of high-titer transient gene expression processes for manufacturing recombinant proteins
Lausanne, EPFL, 2008. DOI : 10.5075/epfl-thesis-4174.* Accelerating the development time of recombinant mammalian cell lines producing high titers of protein therapeutics
Lausanne, EPFL, 2008. DOI : 10.5075/epfl-thesis-4128.* Sustained activation and tumor targeting of NKT cells using a CD1d-anti-HER2-scFv fusion protein induce antitumor effects in mice
Journal of Clinical Investigation. 2008. DOI : 10.1172/JCI33249.* High-titer, serum-free production of adeno-associated virus vectors by polyethyleneimine-mediated plasmid transfection in mammalian suspension cells
Biotechnology Letters. 2007. DOI : 10.1007/s10529-007-9441-3.* Manufacturing of Biopharmaceuticals and Implications for Biosimilars
Kidney and Blood Pressure Research. 2007. DOI : 10.1159/000107094.* Novel orbital shake bioreactors for transient production of CHO derived IgGs
Biotechnology Progress. 2007. DOI : 10.1021/bp070219i.* High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI
Biotechnology and Bioengineering. 2007. DOI : 10.1002/bit.21596.* Bioreactor processes based on disposable materials for the production of recombinant proteins from mammalian cells
Lausanne, EPFL, 2007. DOI : 10.5075/epfl-thesis-3947.* Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems
Biotechnol Lett. 2007. DOI : 10.1007/s10529-006-9298-x.* Recombinant protein production by large-scale transient gene expression in mammalian cells : state of the art and future perspectives
Biotechnology Letters. 2007. DOI : 10.1007/s10529-006-9297-y.* Microfabricated solenoids and Helmholtz coils for NMR spectroscopy of mammalian cells
Lab on a chip. 2007. DOI : 10.1039/b614044k.* NMR spectroscopy and perfusion of mammalian cells using surface microprobes
Lab on a Chip. 2007. DOI : 10.1039/B613240E.* New disposable tubes for rapid and precise biomass assessment for suspension cultures of mammalian cells
Biotechnol Bioeng. 2006. DOI : 10.1002/bit.21071.* Disassembly of polyethylenimine-DNA particles in vitro: implications for polyethylenimine-mediated DNA delivery
J Control Release. 2006. DOI : 10.1016/j.jconrel.2006.09.006.* Multiple glycerol shocks increase the calcium phosphate transfection of non-synchronized CHO cells
Biotechnology letters. 2006. DOI : 10.1007/s10529-006-9167-7.* Genetic characterization of CHO production host DG44 and derivative recombinant cell lines
Biochemical and biophysical research communications. 2006. DOI : 10.1016/j.bbrc.2005.12.111.* Generation of recombinant Chinese hamster ovary cell lines by microinjection
Biotechnology letters. 2006. DOI : 10.1007/s10529-005-6062-6.* Polyethylenimine-based quality control assay for plasmid DNA
Analytical biochemistry. 2006. DOI : 10.1016/j.ab.2006.05.002.* The use of RNA interference to increase PEI-mediated transient gene expression in mammalian cells
Lausanne, EPFL, 2006. DOI : 10.5075/epfl-thesis-3450.* Low-temperature pausing of cultivated mammalian cells
Biotechnol Bioeng. 2005. DOI : 10.1002/bit.20320.* Mammalian cell culture productivity at large scale – from grams/liter to tens of grams/liter soon?
2005. p. 509-512.* Engineered Streptomyces quorum-sensing components enable inducible siRNA-mediated translation control in mammalian cells and adjustable transcription control in mice
journal of gene medicine. 2005. DOI : 10.1002/jgm.682.* Orbital shaker technology for the cultivation of mammalian cells in suspension
Biotechnology and bioengineering. 2005. DOI : 10.1002/bit.20358.* Large-scale transient expression of therapeutic proteins in mammalian cells
Therapeutic Proteins: Methods and Protocols; Totowa, New jersey: Humana Press, 2005. p. 87-98.* The CELO adenovirus Gam1 protein enhances transient and stable recombinant protein expression in Chinese hamster ovary cells
Journal of biotechnology. 2005. DOI : 10.1016/j.jbiotec.2005.01.006.* Transient gene expression in suspension HEK-293 cells: application to large-scale protein production
Biotechnology progress. 2005. DOI : 10.1021/bp049830x.* Transient gene expression for rapid protein production
Lausanne, EPFL, 2005. DOI : 10.5075/epfl-thesis-3297.* Establishment of recombinant mammalian cell lines
Lausanne, EPFL, 2005. DOI : 10.5075/epfl-thesis-3150.* Natural anti-FcepsilonRIalpha autoantibodies may interfere with diagnostic tests for autoimmune urticaria
J Autoimmun. 2004. DOI : 10.1016/j.jaut.2003.09.007.* Specific recognition of the viral protein UL18 by CD85j/LIR-1/ILT2 on CD8+ T cells mediates the non-MHC-restricted lysis of human cytomegalovirus-infected cells
Journal of Immunology. 2004. DOI : 10.4049/jimmunol.172.9.5629.* Tubespin satellites: a fast track approach for process development with animal cells using shaking technology
Biochemical Engineering Journal. 2004. DOI : 10.1016/S1369-703X(03)00180-3.* Production of recombinant protein therapeutics in cultivated mammalian cells
Nature biotechnology. 2004. DOI : 10.1038/nbt1026.* Transfection of adherent and suspended cells by calcium phosphate
Methods. 2004. DOI : 10.1016/j.ymeth.2003.11.011.* Reduction of adenovirus E1A mRNA by RNAi results in enhanced recombinant protein expression in transiently transfected HEK293 cells
Gene. 2004. DOI : 10.1016/j.gene.2004.06.054.* Serum-free large-scale transient transfection of CHO cells
Biotechnology and bioengineering. 2004. DOI : 10.1002/bit.20161.* A spectrophotometric assay for the quantification of polyethylenimine in DNA nanoparticles
Analytical biochemistry. 2004. DOI : 10.1016/j.ab.2004.07.020.* Calfection: a novel gene transfer method for suspension cells
Biochimica et biophysica acta. 2004. DOI : 10.1016/j.bbaexp.2003.11.016.* A method for introducing a nucleic acid into a eukaryotic cell
WO03000908 ; EP1270733 . 2003.* Transient gene expression in suspension HEK-293 cells: application to large-scale protein production
2003. p. 365-367. DOI : 10.1007/1-4020-3103-3_71.* Towards an affinity-based capture system for the isolation of high expression cells using a co-expressed surface protein
2003. p. 471-473. DOI : 10.1007/1-4020-3103-3_90.* Gene transfer and gene amplification in mammalian cells
Gene Transfer and Expression in Mammalian Cells; Amsterdam: Elsevier Science, 2003. p. 309-335.* Co-transfer of multiple plasmids/viruses as an attractive method to introduce several genes in mammalian cells
New Comprehensive Biochemistry; 2003. p. 337-357.* Human therapeutic proteins from silkworms
Nature biotechnology. 2003. DOI : 10.1038/nbt0103-34.* Transfection of partially purified plasmid DNA for high level transient protein expression in HEK293-EBNA cells
Journal of biotechnology. 2003. DOI : 10.1016/S0168-1656(03)00032-4.* Pausing of mammalian cells by cold exposure, limits and opportunities
2003. p. 67-70. DOI : 10.1007/1-4020-3103-3_8.* Molecular approaches to influence epigenetic effectors of transient and stable transgene expression in mammalian cells
2003. p. 15-22. DOI : 10.1007/1-4020-3103-3_2.* Scalable, Serum-free, High Efficiency Transfection of CHO Cells using an Economical DNA Transfer Vehicle
2003. p. 629-632. DOI : 10.1007/1-4020-3103-3_127.* Gene Transfer to animal cells in culture by microinjection: A novel tool to create recombinant cell lines?
2003. p. 509-512. DOI : 10.1007/1-4020-3103-3_99.* Correlation between cell cycle and calcium phosphate transient transfection of CHO cells
Lausanne, EPFL, 2003. DOI : 10.5075/epfl-thesis-2829.* Affinity macroligands
EP1237940 ; US6258275 ; AU7540900 ; WO0125287 . 2002.* Cells, genetically modified with optical marker
US6509174 ; US2002058334 ; EP1088058 ; WO9966032 ; AU4054399 ; WO9966032 . 2002.* Transfection using plasmid preparations
WO0226966 ; AU9014701 ; WO0226966 . 2002.* 100-liter transient transfection
Cytotechnology. 2002. DOI : 10.1023/A:1021173124640.* S-phase synchronized CHO cells show elevated transfection efficiency and expression using CaPi
Cytotechnology. 2002. DOI : 10.1023/A:1021197830091.* Transient gene expression: A novel mammalian cell-based technology for recombinant protein production. A historical and technical perspective
2002. p. 83-88. DOI : 10.1007/978-94-017-0726-8_15.* Mammalian Cell Culture
The Encyclopedia of Physical Science and Technology; San Diego, Calif: Academic Press, 2002. p. 31-47.* Human urothelial cells grown on collagen adsorbed to surface-modified polymers
Urology. 2002. DOI : 10.1016/S0090-4295(02)01642-4.* Balancing GFP reporter plasmid quantity in large-scale transient transfections for recombinant anti-human Rhesus-D IgG1 synthesis
Biotechnology and bioengineering. 2002. DOI : 10.1002/bit.10309.* Fluorescent proteins in animal cells for process development: optimization of sodium butyrate treatment as an example
Biotechnology and bioengineering. 2002. DOI : 10.1002/bit.10108.* Acrylic acid grafting and collagen immobilization on poly(ethylene terephthalate) surfaces for adherence and growth of human bladder smooth muscle cells
Biomaterials. 2002. DOI : 10.1016/S0142-9612(02)00061-3.* Generation of plasmid DNA for the application of transient transfection in mammalian cells
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| Paco Pino, Joeri Kint, Divor Kiseljak, Valentina Agnolon, Giampietro Corradin, Andrey V. Kajava, Paolo Rovero, Ronald Dijkman, Gerco den Hartog, Jason S. McLellan, Patric O. Byrne, Maria J. Wurm, Florian M. Wurm Processes 2020, 8(12), 1539 |
Trimeric SARS-CoV-2 Spike Proteins Produced from CHO Cells in Bioreactors are High-Quality Antigens |