Florian Maria Wurm
Publications représentatives
Production of recombinant protein therapeutics in cultivated mammalian cells
Florian M. Wurm
Published in Nature Biotechnology 22,11, 1393-1398 in 2004
CHO Quasispecies - Implications for Manufacturing Processes
Florian Maria Wurm
Published in Processes 2013, 1, 296-311 in 2013
* High-Titer Neutralizing Antibodies against the SARS-CoV-2 Delta Variant Induced by Alhydroxyquim-II-Adjuvanted Trimeric Spike Antigens
Microbiology Spectrum. 2022-02-01. DOI : 10.1128/spectrum.01695-21.* Fluid dynamics of a pilot-scale OrbShake bioreactor under different operating conditions
Journal Of Chemical Technology And Biotechnology. 2022. DOI : 10.1002/jctb.6995.* Boosted Pro-Inflammatory Activity in Human PBMCs by Lipopolysaccharide and SARS-CoV-2 Spike Protein Is Regulated by alpha-1 Antitrypsin
International Journal Of Molecular Sciences. 2021-08-01. DOI : 10.3390/ijms22157941.* Considerations of the Impacts of Cell-Specific Growth and Production Rate on Clone Selection-A Simulation Study
Processes. 2021-06-01. DOI : 10.3390/pr9060964.* Naming CHO cells for bio-manufacturing: Genome plasticity and variant phenotypes of cell populations in bioreactors question the relevance of old names
Biotechnology Journal. 2021-06-25. DOI : 10.1002/biot.202100165.* Asymmetric opening of the homopentameric 5-HT3A serotonin receptor in lipid bilayers
Nature Communications. 2021-02-16. DOI : 10.1038/s41467-021-21016-7.* Trimeric SARS-CoV-2 Spike Proteins Produced from CHO Cells in Bioreactors Are High-Quality Antigens
Processes. 2020-12-01. DOI : 10.3390/pr8121539.* Designs and Characterization of Subunit Ebola GP Vaccine Candidates: Implications for Immunogenicity
Frontiers In Immunology. 2020-11-04. DOI : 10.3389/fimmu.2020.586595.* Analysis of volumetric mass transfer coefficient (k(L)a) in small- (250 mL) to large-scale (2500 L) orbitally shaken bioreactors
3 Biotech. 2020-08-19. DOI : 10.1007/s13205-020-02352-9.* The Delivery of alpha 1-Antitrypsin Therapy Through Transepidermal Route: Worthwhile to Explore
Frontiers In Pharmacology. 2020-07-03. DOI : 10.3389/fphar.2020.00983.* Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells
Molecular Therapy-Methods & Clinical Development. 2019-06-14. DOI : 10.1016/j.omtm.2018.11.004.* Recombinant Proteins for Industrial versus Pharmaceutical Purposes: A Review of Process and Pricing
Processes. 2019-08-01. DOI : 10.3390/pr7080476.* Contactless Respiration Monitoring in Real-Time via a Video Camera
2018-01-01. Joint Conference of the European Medical and Biological Engineering Conference (EMBEC) / Nordic-Baltic Conference on Biomedical Engineering and Medical Physics (NBC), Tampere, FINLAND, Jun, 2017. p. 567-570. DOI : 10.1007/978-981-10-5122-7_142.* Improved process conditions for increasing expression of MHC class II protein from a stable Drosophila S2 cell line
BIOTECHNOLOGY LETTERS. 2018. DOI : 10.1007/s10529-017-2440-0.* Fluid dynamics of flow fields in a disposable 600-mL orbitally shaken bioreactor
Biochemical Engineering Journal. 2018. DOI : 10.1016/j.bej.2017.10.019.* Cloning of CHO Cells, Productivity and Genetic Stability—A Discussion
Processes. 2017-04-20. DOI : 10.3390/pr5020020.* Validation of Scalable and Compliant AAV Production
2017. 20th Annual Meeting of the American-Society-of-Gene-and-Cell-Therapy (ASGCT), Washington, DC, MAY 10-13, 2017. p. 326-326.* Studies on fluid dynamics of the flow field and gas transfer in orbitally shaken tubes
Biotechnology Progress. 2017. DOI : 10.1002/btpr.2375.* Multigene expression in stable CHO cell pools generated with the piggyBac transposon system
Biotechnology Progress. 2016. DOI : 10.1002/btpr.2319.* A comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation
Biotechnology Progress. 2016. DOI : 10.1002/btpr.2328.* Recombinant protein production from stable mammalian cell lines and pools
Current Opinion In Structural Biology. 2016. DOI : 10.1016/j.sbi.2016.06.005.* Suspension-Adapted HEK 293 Cells in Orbital Shaken Bioreactors for the Production of Adeno-Associated Virus Vectors
2016. 19th Annual Meeting of the American-Society-of-Gene-and-Cell-Therapy (ASGCT), Washington DC, USA, May 4-7, 2016. p. S183-S184.* Comparison of Three Transposons for the Generation of Highly Productive Recombinant CHO Cell Pools and Cell Lines
Biotechnology And Bioengineering. 2016. DOI : 10.1002/bit.25888.* Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension
Analytical Biochemistry. 2016. DOI : 10.1016/j.ab.2016.04.009.* Enhanced Plasmid DNA Utilization in Transiently Transfected CHO-DG44 Cells in the Presence of Polar Solvents
Biotechnology Progress. 2015. DOI : 10.1002/btpr.2152.* A simple plasmid-based transient gene expression method using High Five cells
Journal Of Biotechnology. 2015. DOI : 10.1016/j.jbiotec.2015.10.007.* Production of active glycosylation-deficient gamma-secretase complex for crystallization studies
Biotechnology And Bioengineering. 2015. DOI : 10.1002/bit.25675.* Transient Production of Recombinant Adeno-Associated Virus (AAV) Vectors for Gene Therapy Applications Using Suspension-Adapted HEK 293 Cells in Orbital Shaken Bioreactors
2015. 18th Annual Meeting of the American-Society-of-Gene-and-Cell-Therapy (ASGCT)'. p. S184-S185.* Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools
Journal of Biotechnology. 2015. DOI : 10.1016/j.jbiotec.2015.03.001.* Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells
Biotechnology Progress. 2015. DOI : 10.1002/btpr.2064.* Study of transposon-mediated cell pool and cell line generation in CHO cells
Lausanne, EPFL, 2015. DOI : 10.5075/epfl-thesis-6563.* Development of plasmid-based expression technology for rapid and sustained production of recombinant proteins in insect cells
Lausanne, EPFL, 2014. DOI : 10.5075/epfl-thesis-6435.* Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells
International journal of molecular and cellular medicine. 2014.* The Engineering of a Scalable Disposable Orbitally Shaken Bioreactor System for Animal Cell Cultivation and Therapeutic Protein Production
Lausanne, EPFL, 2014. DOI : 10.5075/epfl-thesis-6423.* Surface wave dynamics in orbital shaken cylindrical containers
Physics of Fluids. 2014. DOI : 10.1063/1.4874612.* Virus-free transient protein production in Sf9 cells
Journal Of Biotechnology. 2014. DOI : 10.1016/j.jbiotec.2013.11.018.* CHO Quasispecies—Implications for Manufacturing Processes
Processes. 2013. DOI : 10.3390/pr1030296.* The optimization of a rapid low-cost alternative of large-scale medium sterilization
2013. 23rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health, Lille, France, 23-26 June 2013. p. P45. DOI : 10.1186/1753-6561-7-S6-P45.* Study of the improved Sf9 transient gene expression process
2013. 23rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health, Lille, France, 23-26 June 2013. p. P19. DOI : 10.1186/1753-6561-7-S6-P19.* Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells
Protein Expression and Purification. 2013. DOI : 10.1016/j.pep.2013.09.001.* High expression of the aspartate–glutamate carrier Aralar1 favors lactate consumption in CHO cell culture
Pharmaceutical Bioprocessing. 2013. DOI : 10.4155/pbp.13.5.* Disposable 600-mL orbitally shaken bioreactor for mammalian cell cultivation in suspension
Biochemical Engineering Journal. 2013. DOI : 10.1016/j.bej.2013.04.008.* Numerical simulation of orbitally shaken viscous fluids with free surface
International Journal For Numerical Methods In Fluids. 2013. DOI : 10.1002/fld.3658.* Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures
Processes. 2013. DOI : 10.3390/pr1010003.* DNA delivery with hyperbranched polylysine: A comparative study with linear and dendritic polylysine
Journal of Controlled Release. 2013. DOI : 10.1016/j.jconrel.2013.01.019.* Highly efficient production of the Alzheimer's γ-Secretase integral membrane protease complex by a multi-gene stable integration approach
Biotechnology and Bioengineering. 2013. DOI : 10.1002/bit.24851.* A Simple High Yielding Transient Gene Expression System for CHO Cells
Lausanne, EPFL, 2013. DOI : 10.5075/epfl-thesis-5572.* The Use of Valproic Acid in Transient Gene Expression with HEK-293E Cells
Lausanne, EPFL, 2013. DOI : 10.5075/epfl-thesis-5498.* Lactate metabolism shift in CHO cell culture: the role of mitochondrial oxidative activity
New Biotechnology. 2013. DOI : 10.1016/j.nbt.2012.05.021.* Comparative Study on the In Vitro Cytotoxicity of Linear, Dendritic, and Hyperbranched Polylysine Analogues
Biomacromolecules. 2012. DOI : 10.1021/bm300930j.* A NanoDrop-based method for rapid determination of viability decline in suspension cultures of animal cells
Analytical Biochemistry. 2012. DOI : 10.1016/j.ab.2012.08.028.* Multidisciplinary Analysis of the Metabolic Shift to Lactate Consumption in CHO Cell Culture
Lausanne, EPFL, 2012. DOI : 10.5075/epfl-thesis-5420.* Power input correlation to characterize the hydrodynamics of cylindrical orbitally shaken bioreactors
Biochemical Engineering Journal. 2012. DOI : 10.1016/j.bej.2012.04.007.* Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells
Biotechnology and Bioengineering. 2012. DOI : 10.1002/bit.24494.* Poly(ethyleneimine)-Mediated Large-Scale Transient Gene Expression: Influence of Molecular Weight, Polydispersity and N-Propionyl Groups
Macromolecular Bioscience. 2012. DOI : 10.1002/mabi.201100404.* k(L)a as a predictor for successful probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors
New biotechnology. 2012. DOI : 10.1016/j.nbt.2011.10.010.* Reduced glutamine concentration improves protein production in growth-arrested CHO-DG44 and HEK-293E cells
Biotechnology letters. 2012. DOI : 10.1007/s10529-011-0809-z.* Flexible antibodies with nonprotein hinges
Proceedings Of The Japan Academy Series B-Physical And Biological Sciences. 2011. DOI : 10.2183/pjab.87.603.* Hydrodynamic stress in orbitally shaken bioreactors
2011. p. P39. DOI : 10.1186/1753-6561-5-S8-P39.* Transient gene expression with CHO cells in conditioned medium: a study using TubeSpin® bioreactors
2011. p. P38. DOI : 10.1186/1753-6561-5-S8-P38.* Transient transfection of insect Sf-9 cells in TubeSpin® bioreactor 50 tubes
2011. p. P37. DOI : 10.1186/1753-6561-5-S8-P37.* Influence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells
2011. p. P35. DOI : 10.1186/1753-6561-5-S8-P35.* Rapid recombinant protein production from pools of transposon-generated CHO cells
2011. p. P34. DOI : 10.1186/1753-6561-5-S8-P34.* The use of filler DNA for improved transfection and reduced DNA needs in transient gene expression with CHO and HEK cells
2011. p. P33. DOI : 10.1186/1753-6561-5-S8-P33.* Transposon mediated co-integration and co-expression of transgenes in CHO-DG44 cells
2011. p. P32. DOI : 10.1186/1753-6561-5-S8-P32.* CHO cell lines generated by PiggyBac transposition
2011. p. P31. DOI : 10.1186/1753-6561-5-S8-P31.* Structural analysis of intact monoclonal antibodies by electron transfer dissociation mass spectrometry
Analytical chemistry. 2011. DOI : 10.1021/ac201293m.* Mobile phone-induced honeybee worker piping
Apidologie. 2011. DOI : 10.1007/s13592-011-0016-x.* Large-Scale Transfection of Mammalian Cells
Protein Expression in Mammalian Cells; Humana Press, 2011. p. 13-26.* First CHO genome
Nature Biotechnology. 2011. DOI : 10.1038/nbt.1943.* The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability
Biotechnology and Bioengineering. 2011. DOI : 10.1002/bit.23167.* A simple high-yielding process for transient gene expression in CHO cells
Journal of Biotechnology. 2011. DOI : 10.1016/j.jbiotec.2011.03.001.* OrbShake Bioreactors for Mammalian Cell Cultures
Lausanne, EPFL, 2011. DOI : 10.5075/epfl-thesis-5135.* Efficient and reproducible mammalian cell bioprocesses without probes and controllers?
New biotechnology. 2011. DOI : 10.1016/j.nbt.2011.02.004.* Manufacturing recombinant proteins in kg-ton quantities using animal cells in bioreactors
European Journal of Pharmaceutics and Biopharmaceutics. 2011. DOI : 10.1016/j.ejpb.2011.01.005.* TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension
Biotechnology Letters. 2011. DOI : 10.1007/s10529-011-0527-6.* Role of polymer architecture on polycation induced cell death: systematic study on molecular mechanism
2010. 3rd International Cellular Delivery of Therapeutic Macromolecules (CDTM) Symposium, Cardiff, ENGLAND, Jun 26-29, 2010. p. 1086-1086. DOI : 10.1016/j.drudis.2010.09.369.* Innovative, Non-stirred Bioreactors in Scales from Milliliters up to 1000 Liters for Suspension Cultures of Cells using Disposable Bags and Containers – A Swiss Contribution
CHIMIA International Journal for Chemistry. 2010. DOI : 10.2533/chimia.2010.819.* Generation of stable, high-producing cho cell lines by lentiviral vector-mediated gene transfer in serum-free suspension culture
Biotechnology and Bioengineering. 2010. DOI : 10.1002/bit.22968.* Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells
Journal of biotechnology. 2010. DOI : 10.1016/j.jbiotec.2010.05.003.* Lentiviral Vector-Mediated Gene Delivery for the Generation of Chinese Hamster Ovary Cell Lines with Improved Productivity and Stability
Lausanne, EPFL, 2010. DOI : 10.5075/epfl-thesis-4834.* Hyperosmolarity enhances transient recombinant protein yield in Chinese hamster ovary cells
Biotechnology Letters. 2010. DOI : 10.1007/s10529-010-0331-8.* Transient Gene Expression in HEK-293E Cells for Recombinant Protein Production
Lausanne, EPFL, 2010. DOI : 10.5075/epfl-thesis-4815.* Determination of a scale-up factor from mixing time studies in orbitally shaken bioreactors
Biochemical Engineering Journal. 2010. DOI : 10.1016/j.bej.2010.08.005.* Compressed collagen gel: a novel scaffold for human bladder cells
Journal of Tissue Engineering and Regenerative Medicine. 2010. DOI : 10.1002/term.222.* Isolation and evaluation of novel adeno-associated virus sequences from porcine tissues
Gene Therapy. 2009. DOI : 10.1038/gt.2009.82.* Transient Gene Expression in Mammalian Cells: Promises and Challenges for Medical Biotechnology
Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology; Hoboken, N.J: Wiley, 2009.* Orbitally shaken bioreactors for mammalian cell culture
Lausanne, EPFL, 2009. DOI : 10.5075/epfl-thesis-4492.* DNA delivery by microinjection for the generation of recombinant mammalian cell lines
Microinjection: Methods and Applications; Humana Press, 2009. p. 99-112.* 25 years of recombinant proteins from reactor-grown cells — Where do we go from here?
Biotechnology Advances. 2009. DOI : 10.1016/j.biotechadv.2009.05.008.* Use of Orbital Shaken Disposable Bioreactors for Mammalian Cell Cultures from the Milliliter-Scale to the 1,000-Liter Scale
Advances in Biochemical Engineering / Biotechnology. 2009. DOI : 10.1007/10_2008_18.* Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells
Vaccine. 2009. DOI : 10.1016/j.vaccine.2009.06.019.* Studies on scaffold design for tissue engineering applications
Lausanne, EPFL, 2009. DOI : 10.5075/epfl-thesis-4445.* Suspension-adapted Chinese hamster ovary-derived cells expressing green fluorescent protein as a screening tool for biomaterials
Biotechnol Lett. 2009. DOI : 10.1007/s10529-009-9997-1.* Transient recombinant protein expression in mammalian cells
Lausanne, EPFL, 2009. DOI : 10.5075/epfl-thesis-4393.* Production and characterisation of rationally optimised, anti-melanoma T-cell receptors: a step closer towards immunotherapeutic treatment for patients
2008. Annual joint meeting of the Swiss Societies for Pneumology, Paediatric Pneumology, Allergology and Immunology, Thoracic Surgery, Fribourg, April 17-18, 2008. p. 44S.* Recombinant human collagen as a bioactive element in scaffolds for tissue engineering
Annual Tissue-Engineering-and-Regenerative-Medicine-International-Society-European-Chapter Meeting, Porto, PORTUGAL, Jun, 2008.* Compressed collagen-PLA hybrids as scaffold for human bladder cells
Annual Tissue-Engineering-and-Regenerative-Medicine-International-Society-European-Chapter Meeting, Porto, PORTUGAL, Jun, 2008.* The kinetics of polyethylenimine-mediated transfection in suspension cultures of Chinese hamster ovary cells
Molecular Biotechnology. 2008. DOI : 10.1007/s12033-008-9069-0.* Recombinant therapeutic protein production in cultivated mammalian cells: current status and future prospects
Drug Discovery Today: Technologies. 2008. DOI : 10.1016/j.ddtec.2008.12.003.* Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells
Biotechnology Progress. 2008. DOI : 10.1021/bp070286c.* Valproic acid: A viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures
Biotechnology and Bioengineering. 2008. DOI : 10.1002/bit.21882.* Shaken helical track bioreactors: Providing oxygen to high-density cultures of mammalian cells at volumes up to 1000L by surface aeration with air
New Biotechnology. 2008. DOI : 10.1016/j.nbt.2008.03.001.* Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions
Nucleic Acids Research. 2008. DOI : 10.1093/nar/gkn423.* Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection
Biotechnology and Bioengineering. 2008. DOI : 10.1002/bit.21972.* Coexpression of acidic fibroblast growth factor enhances specific productivity and antibody titers in transiently transfected HEK293 cells
New Biotechnology. 2008. DOI : 10.1016/j.nbt.2008.08.007.* Development of high-titer transient gene expression processes for manufacturing recombinant proteins
Lausanne, EPFL, 2008. DOI : 10.5075/epfl-thesis-4174.* Accelerating the development time of recombinant mammalian cell lines producing high titers of protein therapeutics
Lausanne, EPFL, 2008. DOI : 10.5075/epfl-thesis-4128.* Sustained activation and tumor targeting of NKT cells using a CD1d-anti-HER2-scFv fusion protein induce antitumor effects in mice
Journal of Clinical Investigation. 2008. DOI : 10.1172/JCI33249.* High-titer, serum-free production of adeno-associated virus vectors by polyethyleneimine-mediated plasmid transfection in mammalian suspension cells
Biotechnology Letters. 2007. DOI : 10.1007/s10529-007-9441-3.* Manufacturing of Biopharmaceuticals and Implications for Biosimilars
Kidney and Blood Pressure Research. 2007. DOI : 10.1159/000107094.* Novel orbital shake bioreactors for transient production of CHO derived IgGs
Biotechnology Progress. 2007. DOI : 10.1021/bp070219i.* High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI
Biotechnology and Bioengineering. 2007. DOI : 10.1002/bit.21596.* Bioreactor processes based on disposable materials for the production of recombinant proteins from mammalian cells
Lausanne, EPFL, 2007. DOI : 10.5075/epfl-thesis-3947.* Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems
Biotechnol Lett. 2007. DOI : 10.1007/s10529-006-9298-x.* Recombinant protein production by large-scale transient gene expression in mammalian cells : state of the art and future perspectives
Biotechnology Letters. 2007. DOI : 10.1007/s10529-006-9297-y.* Microfabricated solenoids and Helmholtz coils for NMR spectroscopy of mammalian cells
Lab on a chip. 2007. DOI : 10.1039/b614044k.* NMR spectroscopy and perfusion of mammalian cells using surface microprobes
Lab on a Chip. 2007. DOI : 10.1039/B613240E.* New disposable tubes for rapid and precise biomass assessment for suspension cultures of mammalian cells
Biotechnol Bioeng. 2006. DOI : 10.1002/bit.21071.* Disassembly of polyethylenimine-DNA particles in vitro: implications for polyethylenimine-mediated DNA delivery
J Control Release. 2006. DOI : 10.1016/j.jconrel.2006.09.006.* Multiple glycerol shocks increase the calcium phosphate transfection of non-synchronized CHO cells
Biotechnology letters. 2006. DOI : 10.1007/s10529-006-9167-7.* Genetic characterization of CHO production host DG44 and derivative recombinant cell lines
Biochemical and biophysical research communications. 2006. DOI : 10.1016/j.bbrc.2005.12.111.* Generation of recombinant Chinese hamster ovary cell lines by microinjection
Biotechnology letters. 2006. DOI : 10.1007/s10529-005-6062-6.* Polyethylenimine-based quality control assay for plasmid DNA
Analytical biochemistry. 2006. DOI : 10.1016/j.ab.2006.05.002.* The use of RNA interference to increase PEI-mediated transient gene expression in mammalian cells
Lausanne, EPFL, 2006. DOI : 10.5075/epfl-thesis-3450.* Low-temperature pausing of cultivated mammalian cells
Biotechnol Bioeng. 2005. DOI : 10.1002/bit.20320.* Mammalian cell culture productivity at large scale – from grams/liter to tens of grams/liter soon?
2005. p. 509-512.* Engineered Streptomyces quorum-sensing components enable inducible siRNA-mediated translation control in mammalian cells and adjustable transcription control in mice
journal of gene medicine. 2005. DOI : 10.1002/jgm.682.* Orbital shaker technology for the cultivation of mammalian cells in suspension
Biotechnology and bioengineering. 2005. DOI : 10.1002/bit.20358.* Large-scale transient expression of therapeutic proteins in mammalian cells
Therapeutic Proteins: Methods and Protocols; Totowa, New jersey: Humana Press, 2005. p. 87-98.* The CELO adenovirus Gam1 protein enhances transient and stable recombinant protein expression in Chinese hamster ovary cells
Journal of biotechnology. 2005. DOI : 10.1016/j.jbiotec.2005.01.006.* Transient gene expression in suspension HEK-293 cells: application to large-scale protein production
Biotechnology progress. 2005. DOI : 10.1021/bp049830x.* Transient gene expression for rapid protein production
Lausanne, EPFL, 2005. DOI : 10.5075/epfl-thesis-3297.* Establishment of recombinant mammalian cell lines
Lausanne, EPFL, 2005. DOI : 10.5075/epfl-thesis-3150.* Natural anti-FcepsilonRIalpha autoantibodies may interfere with diagnostic tests for autoimmune urticaria
J Autoimmun. 2004. DOI : 10.1016/j.jaut.2003.09.007.* Specific recognition of the viral protein UL18 by CD85j/LIR-1/ILT2 on CD8+ T cells mediates the non-MHC-restricted lysis of human cytomegalovirus-infected cells
Journal of Immunology. 2004. DOI : 10.4049/jimmunol.172.9.5629.* Tubespin satellites: a fast track approach for process development with animal cells using shaking technology
Biochemical Engineering Journal. 2004. DOI : 10.1016/S1369-703X(03)00180-3.* Production of recombinant protein therapeutics in cultivated mammalian cells
Nature biotechnology. 2004. DOI : 10.1038/nbt1026.* Transfection of adherent and suspended cells by calcium phosphate
Methods. 2004. DOI : 10.1016/j.ymeth.2003.11.011.* Reduction of adenovirus E1A mRNA by RNAi results in enhanced recombinant protein expression in transiently transfected HEK293 cells
Gene. 2004. DOI : 10.1016/j.gene.2004.06.054.* Serum-free large-scale transient transfection of CHO cells
Biotechnology and bioengineering. 2004. DOI : 10.1002/bit.20161.* A spectrophotometric assay for the quantification of polyethylenimine in DNA nanoparticles
Analytical biochemistry. 2004. DOI : 10.1016/j.ab.2004.07.020.* Calfection: a novel gene transfer method for suspension cells
Biochimica et biophysica acta. 2004. DOI : 10.1016/j.bbaexp.2003.11.016.* A method for introducing a nucleic acid into a eukaryotic cell
WO03000908 ; EP1270733 . 2003.* Transient gene expression in suspension HEK-293 cells: application to large-scale protein production
2003. p. 365-367. DOI : 10.1007/1-4020-3103-3_71.* Towards an affinity-based capture system for the isolation of high expression cells using a co-expressed surface protein
2003. p. 471-473. DOI : 10.1007/1-4020-3103-3_90.* Gene transfer and gene amplification in mammalian cells
Gene Transfer and Expression in Mammalian Cells; Amsterdam: Elsevier Science, 2003. p. 309-335.* Co-transfer of multiple plasmids/viruses as an attractive method to introduce several genes in mammalian cells
New Comprehensive Biochemistry; 2003. p. 337-357.* Human therapeutic proteins from silkworms
Nature biotechnology. 2003. DOI : 10.1038/nbt0103-34.* Transfection of partially purified plasmid DNA for high level transient protein expression in HEK293-EBNA cells
Journal of biotechnology. 2003. DOI : 10.1016/S0168-1656(03)00032-4.* Pausing of mammalian cells by cold exposure, limits and opportunities
2003. p. 67-70. DOI : 10.1007/1-4020-3103-3_8.* Molecular approaches to influence epigenetic effectors of transient and stable transgene expression in mammalian cells
2003. p. 15-22. DOI : 10.1007/1-4020-3103-3_2.* Scalable, Serum-free, High Efficiency Transfection of CHO Cells using an Economical DNA Transfer Vehicle
2003. p. 629-632. DOI : 10.1007/1-4020-3103-3_127.* Gene Transfer to animal cells in culture by microinjection: A novel tool to create recombinant cell lines?
2003. p. 509-512. DOI : 10.1007/1-4020-3103-3_99.* Correlation between cell cycle and calcium phosphate transient transfection of CHO cells
Lausanne, EPFL, 2003. DOI : 10.5075/epfl-thesis-2829.* Affinity macroligands
EP1237940 ; US6258275 ; AU7540900 ; WO0125287 . 2002.* Cells, genetically modified with optical marker
US6509174 ; US2002058334 ; EP1088058 ; WO9966032 ; AU4054399 ; WO9966032 . 2002.* Transfection using plasmid preparations
WO0226966 ; AU9014701 ; WO0226966 . 2002.* 100-liter transient transfection
Cytotechnology. 2002. DOI : 10.1023/A:1021173124640.* S-phase synchronized CHO cells show elevated transfection efficiency and expression using CaPi
Cytotechnology. 2002. DOI : 10.1023/A:1021197830091.* Transient gene expression: A novel mammalian cell-based technology for recombinant protein production. A historical and technical perspective
2002. p. 83-88. DOI : 10.1007/978-94-017-0726-8_15.* Mammalian Cell Culture
The Encyclopedia of Physical Science and Technology; San Diego, Calif: Academic Press, 2002. p. 31-47.* Human urothelial cells grown on collagen adsorbed to surface-modified polymers
Urology. 2002. DOI : 10.1016/S0090-4295(02)01642-4.* Balancing GFP reporter plasmid quantity in large-scale transient transfections for recombinant anti-human Rhesus-D IgG1 synthesis
Biotechnology and bioengineering. 2002. DOI : 10.1002/bit.10309.* Fluorescent proteins in animal cells for process development: optimization of sodium butyrate treatment as an example
Biotechnology and bioengineering. 2002. DOI : 10.1002/bit.10108.* Acrylic acid grafting and collagen immobilization on poly(ethylene terephthalate) surfaces for adherence and growth of human bladder smooth muscle cells
Biomaterials. 2002. DOI : 10.1016/S0142-9612(02)00061-3.* Generation of plasmid DNA for the application of transient transfection in mammalian cells
Lausanne, EPFL, 2002. DOI : 10.5075/epfl-thesis-2486.* Tissue engineering
Lausanne, EPFL, 2002. DOI : 10.5075/epfl-thesis-2477.* Rapid protein expression in mammalian cells
Lausanne, EPFL, 2002. DOI : 10.5075/epfl-thesis-2473.* Vers un nouveau médicament contre une grave maladie foetale
Flash EPFL. 2001.* Transient gene expression from mammalian cells – a new chapter in animal cell technology?
Cytotechnology. 2001. DOI : 10.1023/A:1013178914052.* Spitzenforschung bleibt möglich
Biotech Forum. 2001.* S-phase synchronized CHO cells show elevated transfection efficiency and expression using Ca/PO4
2001. p. 238-240. DOI : 10.1007/978-94-010-0369-8_55.* Sodium butyrate treatment: a study of critical parameters using CHO cell lines expressing the Green Fluorescent Protein
2001. p. 318-323. DOI : 10.1007/978-94-010-0369-8_72.* Na+ is responsible for intra-run variations of specific productivity of CHO cell lines producing human anti RhD-IgG in bioreactors
2001. p. 193-196. DOI : 10.1007/978-94-010-0369-8_42.* The Influence of pH on Cell Growth and Specific Productivity of Two CHO Cell Lines Producing Human Anti RhD IgG
2001. p. 197-199. DOI : 10.1007/978-94-010-0369-8_43.* Successful Transfection of Partially Purified DNA in the HEK293EBNA Cell Line for Transient Expression
2001. p. 69-71. DOI : 10.1007/978-94-010-0369-8_15.* Extraction of plasmid DNA using reactor scale alkaline lysis and precipitation useful for transient transfection of animal cells
Cytotechnology. 2001. DOI : 10.1023/A:1013106032341.* Calcium phosphate transfection optimization for serum-free suspension culture
Cytotechnology. 2001. DOI : 10.1023/A:1013101927350.* 100 Liter Transient Transfection
2001. p. 37-42. DOI : 10.1007/978-94-010-0369-8_10.* Development of stable cell lines for production or regulated expression using matrix attachment regions
Journal of biotechnology. 2001. DOI : 10.1016/S0168-1656(00)00423-5.* Transient gene expression: recombinant protein production with suspension-adapted HEK293-EBNA cells
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FASEB Journal. 2001. DOI : 10.1096/fj.00-0890hyp.* Small-scale bioreactor system for process development and optimization
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Bioforum International 4. 2000.* Fluorescent mammalian cells
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The Journal of biological chemistry. 1999. DOI : 10.1074/jbc.274.6.3622.* GFP-expressing mammalian cells for fast, sensitive, noninvasive cell growth assessment in a kinetic mode
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Biologicals. 1994. DOI : 10.1006/biol.1994.1015.* Protection against rat endotoxic shock by p55 tumor necrosis factor (TNF) receptor immunoadhesin: comparison with anti-TNF monoclonal antibody
The Journal of infectious diseases. 1994. DOI : 10.1093/infdis/170.5.1323.* A slow clearing, fibrin-specific, PAI-1 resistant variant of t-PA (T103N, KHRR 296-299 AAAA)
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Thrombosis and haemostasis. 1993. DOI : 10.1055/s-0038-1649572.* Involvement of residues 296-299 in the enzymatic activity of tissue-type plasminogen activator
Protein engineering. 1992. DOI : 10.1093/protein/5.3.259.* Integration and stability of CHO amplicons containing plasmid sequences
Developments in biological standardization. 1992.* A region of tissue plasminogen activator that affects plasminogen activation differentially with various fibrin(ogen)-related stimulators
Biochemistry. 1992. DOI : 10.1021/bi00117a016.* High resolution analysis of functional determinants on human tissue-type plasminogen activator
The Journal of biological chemistry. 1991. DOI : 10.1016/S0021-9258(19)67773-2.* Primary structure and biological activity of human brain-derived neurotrophic factor
Endocrinology. 1991. DOI : 10.1210/endo-129-3-1289.* Defective endogenous retrovirus-like sequences and particles of Chinese hamster ovary cells
Developments in biological standardization. 1991.* Existence of two types of integrations
Biologicals. 1991. DOI : 10.1016/1045-1056(91)90028-I.* Biological properties of a CD4 immunoadhesin
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Molecular and cellular biology. 1990. DOI : 10.1128/MCB.10.1.401.* Presence and transcription of intracisternal A-particle-related sequences in CHO cells
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Biologicals. 1990. DOI : 10.1016/1045-1056(90)90002-H.* Protection of chimpanzees from infection by HIV-1 after vaccination with recombinant glycoprotein gp120 but not gp160
Nature. 1990. DOI : 10.1038/345622a0.* Designing CD4 immunoadhesins for AIDS therapy
Nature. 1989. DOI : 10.1038/337525a0.* Use of fluorescence in situ hybridization to detect and monitor transfected and amplified sequences in recombinant CHO cells
Developments in biological standardization. 1989.* Inducible overproduction of the mouse c-myc protein in mammalian cells
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Developments in biological standardization. 1985.* Expression of Herpes simplex virus type 1 glycoprotein C antigens in Escherichia coli
Gene. 1984. DOI : 10.1016/0378-1119(84)90048-9.* Suppression of melanoma development and regression of melanoma in xiphophorine fish after treatment with immune RNA
Cancer research. 1981.Enseignement et PhD
A dirigé les thèses EPFL de
Philippe Pierre Girard, Lisa Hunt, Jason Wright, Isabelle Bisson, Frédéric Grosjean, Madiha Derouazi, Natalie Muller, Martin Bertschinger, Mathieu Benjamin Stettler, Gaurav Backliwal, Sébastien Chenuet, Eva-Maria Balet, Sarah Wulhfard, Xiaowei Zhang, Sophie Nallet, Agata Oberbek, Stéphanie Tissot, Francesca Zagari, Divor Kiseljak, Yashas Rajendra, Xiao Shen, Dominique Terry Monteil Jr, Sowmya Balasubramanian