Florian Maria Wurm
EPFL SV SV-DEC PH-SV
AAB 1 46 (Bâtiment AAB)
Station 19
CH-1015 Lausanne
Biographie
Florian Wurm a obtenu son doctorat en biologie moléculaire et travaux génétiques en 1979 à lUniversité de Giessen en Allemagne.
De 1980 à 1985, il dirigea un groupe de recherche au sein du Département de Virologie de Behringwerke, Marburg en Allemagne.
En 1985, il rejoint la Harvard Medical School à Boston dans le Massachussetts, en tant que hôte académique. De 1986 à 1995, il occupa les fonctions de « Scientist », « Senior Scientist », « Project-Team-Leader » puis « Senior Manager » à Genentech, Inc. à San Francisco, Californie.
En 1995, il est nommé professeur extraordinaire à lEcole Polytechnique Fédérale de Lausanne, puis professeur ordinaire en 1998.
Le professeur Wurm est consultant et membre de plusieurs sociétés scientifiques nationales et internationales, dont ESACT (European Society of Animal Cell Technology).
Il a récemment fondé une start-up en biotechnologie à Monthey en Suisse (ExcellGene SA).
Les recherches quil poursuit actuellement au sein de son laboratoire à lEPFL sont centrées sur le développement de technologies pour lexpression de protéines recombinantes à partir de cellules animales, et plus particulièrement de mammifères.
Publications
Infoscience
Toutes les publications
Les données en-ligne ne sont pas accessibles*
X. Shen; D. Dojcinovic; L. Baldi; D. Hacker; I. Luescher et al. : Improved process conditions for increasing expression of MHC class II protein from a stable Drosophila S2 cell line; BIOTECHNOLOGY LETTERS. 2018. DOI : 10.1007/s10529-017-2440-0.
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L. Zhu; D. T. Monteil; Y. Wang; B. Song; D. L. Hacker et al. : Fluid dynamics of flow fields in a disposable 600-mL orbitally shaken bioreactor; Biochemical Engineering Journal. 2018. DOI : 10.1016/j.bej.2017.10.019.
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F. M. Wurm; M. J. Wurm : Cloning of CHO Cells, Productivity and Genetic Stability-A Discussion; Processes. 2017. DOI : 10.3390/pr5020020.
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D. R. Blessing; G. Vachey; B. Schneider; F. M. Wurm; N. Deglon : Validation of Scalable and Compliant AAV Production. 2017. 20th Annual Meeting of the American-Society-of-Gene-and-Cell-Therapy (ASGCT), Washington, DC, MAY 10-13, 2017. p. 326-326.
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L.-K. Zhu; B.-Y. Song; Z.-L. Wang; D. T. Monteil; X. Shen et al. : Studies on fluid dynamics of the flow field and gas transfer in orbitally shaken tubes; Biotechnology Progress. 2017. DOI : 10.1002/btpr.2375.
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S. Balasubramanian; F. M. Wurm; D. L. Hacker : Multigene expression in stable CHO cell pools generated with the piggyBac transposon system; Biotechnology Progress. 2016. DOI : 10.1002/btpr.2319.
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D. T. Monteil; V. Juvet; J. Paz; M. Moniatte; L. Baldi et al. : A comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation; Biotechnology Progress. 2016. DOI : 10.1002/btpr.2328.
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D. L. Hacker; S. Balasubramanian : Recombinant protein production from stable mammalian cell lines and pools; Current Opinion In Structural Biology. 2016. DOI : 10.1016/j.sbi.2016.06.005.
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D. R. Blessing; B. Schneider; F. M. Wurm; N. Deglon : Suspension-Adapted HEK 293 Cells in Orbital Shaken Bioreactors for the Production of Adeno-Associated Virus Vectors. 2016. 19th Annual Meeting of the American-Society-of-Gene-and-Cell-Therapy (ASGCT), Washington DC, USA, May 4-7, 2016. p. S183-S184.
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S. Balasubramanian; Y. Rajendra; L. Baldi; D. L. Hacker; F. M. Wurm : Comparison of Three Transposons for the Generation of Highly Productive Recombinant CHO Cell Pools and Cell Lines; Biotechnology And Bioengineering. 2016. DOI : 10.1002/bit.25888.
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D. T. Monteil; X. Shen; G. Tontodonati; L. Baldi; D. L. Hacker et al. : Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension; Analytical Biochemistry. 2016. DOI : 10.1016/j.ab.2016.04.009.
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Y. Rajendra; S. Balasubramanian; D. Kiseljak; L. Baldi; F. M. Wurm et al. : Enhanced Plasmid DNA Utilization in Transiently Transfected CHO-DG44 Cells in the Presence of Polar Solvents; Biotechnology Progress. 2015. DOI : 10.1002/btpr.2152.
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X. Shen; A. K. Pitol; V. Bachmann; D. L. Hacker; L. Baldi et al. : A simple plasmid-based transient gene expression method using High Five cells; Journal Of Biotechnology. 2015. DOI : 10.1016/j.jbiotec.2015.10.007.
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A. R. Lopez; M. Dimitrov; H. Gerber; V. Braman; D. L. Hacker et al. : Production of active glycosylation-deficient gamma-secretase complex for crystallization studies; Biotechnology And Bioengineering. 2015. DOI : 10.1002/bit.25675.
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D. R. Blessing; M. Montavon; M. De Jesus; L. Baldi; B. Schneider et al. : Transient Production of Recombinant Adeno-Associated Virus (AAV) Vectors for Gene Therapy Applications Using Suspension-Adapted HEK 293 Cells in Orbital Shaken Bioreactors. 2015. 18th Annual Meeting of the American-Society-of-Gene-and-Cell-Therapy (ASGCT)'. p. S184-S185.
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S. Balasubramanian; M. Matasci; Z. Kadlecova; L. Baldi; D. L. Hacker et al. : Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools; Journal of Biotechnology. 2015. DOI : 10.1016/j.jbiotec.2015.03.001.
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Y. Rajendra; D. Kiseljak; L. Baldi; F. M. Wurm; D. L. Hacker : Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells; Biotechnology Progress. 2015. DOI : 10.1002/btpr.2064.
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S. Balasubramanian / F. M. Wurm; D. Hacker (Dir.) : Study of transposon-mediated cell pool and cell line generation in CHO cells. Lausanne, EPFL, 2015. DOI : 10.5075/epfl-thesis-6563.
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X. Shen / F. M. Wurm; D. Hacker (Dir.) : Development of plasmid-based expression technology for rapid and sustained production of recombinant proteins in insect cells. Lausanne, EPFL, 2014. DOI : 10.5075/epfl-thesis-6435.
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F. Davami; L. Baldi; Y. Rajendra; F. M Wurm : Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells; International journal of molecular and cellular medicine. 2014.
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D. T. Monteil Jr / F. M. Wurm; D. Hacker (Dir.) : The Engineering of a Scalable Disposable Orbitally Shaken Bioreactor System for Animal Cell Cultivation and Therapeutic Protein Production. Lausanne, EPFL, 2014. DOI : 10.5075/epfl-thesis-6423.
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M. Reclari; M. Dreyer; S. Tissot; D. Obreschkow; F. M. Wurm et al. : Surface wave dynamics in orbital shaken cylindrical containers; Physics of Fluids. 2014. DOI : 10.1063/1.4874612.
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X. Shen; D. L. Hacker; L. Baldi; F. M. Wurm : Virus-free transient protein production in Sf9 cells; Journal Of Biotechnology. 2014. DOI : 10.1016/j.jbiotec.2013.11.018.
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F. Wurm : CHO Quasispecies—Implications for Manufacturing Processes; Processes. 2013. DOI : 10.3390/pr1030296.
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D. T. Monteil; C. A. Bürki; L. Baldi; D. L. Hacker; M. De Jesus et al. : The optimization of a rapid low-cost alternative of large-scale medium sterilization. 2013. 23rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health, Lille, France, 23-26 June 2013. p. P45. DOI : 10.1186/1753-6561-7-S6-P45.
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X. Shen; D. L. Hacker; L. Baldi; F. M. Wurm : Study of the improved Sf9 transient gene expression process. 2013. 23rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health, Lille, France, 23-26 June 2013. p. P19. DOI : 10.1186/1753-6561-7-S6-P19.
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D. L. Hacker; D. Kiseljak; Y. Rajendra; S. Thurnheer; L. Baldi et al. : Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells; Protein Expression and Purification. 2013. DOI : 10.1016/j.pep.2013.09.001.
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F. Zagari; M. Stettler; L. Baldi; H. Broly; F. M. Wurm et al. : High expression of the aspartate–glutamate carrier Aralar1 favors lactate consumption in CHO cell culture; Pharmaceutical Bioprocessing. 2013. DOI : 10.4155/pbp.13.5.
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D. T. Monteil; G. Tontodonati; S. Ghimire; L. Baldi; D. L. Hacker et al. : Disposable 600-mL orbitally shaken bioreactor for mammalian cell cultivation in suspension; Biochemical Engineering Journal. 2013. DOI : 10.1016/j.bej.2013.04.008.
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M. Discacciati; D. Hacker; A. Quarteroni; S. Quinodoz; S. Tissot et al. : Numerical simulation of orbitally shaken viscous fluids with free surface; International Journal For Numerical Methods In Fluids. 2013. DOI : 10.1002/fld.3658.
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S. Tissot; P. Michel; C. Douet; S. Grezet; L. Baldi et al. : Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures; Processes. 2013. DOI : 10.3390/pr1010003.
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Z. Kadlecova; Y. Rajendra; M. Matasci; L. Baldi; D. L. Hacker et al. : DNA delivery with hyperbranched polylysine: A comparative study with linear and dendritic polylysine; Journal of Controlled Release. 2013. DOI : 10.1016/j.jconrel.2013.01.019.
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J.-R. Alattia; M. Matasci; M. Dimitrov; L. Aeschbach; S. Balasubramanian et al. : Highly efficient production of the Alzheimer's γ-Secretase integral membrane protease complex by a multi-gene stable integration approach; Biotechnology and Bioengineering. 2013. DOI : 10.1002/bit.24851.
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Y. Rajendra / F. M. Wurm; D. Hacker (Dir.) : A Simple High Yielding Transient Gene Expression System for CHO Cells. Lausanne, EPFL, 2013. DOI : 10.5075/epfl-thesis-5572.
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D. Kiseljak / F. M. Wurm (Dir.) : The Use of Valproic Acid in Transient Gene Expression with HEK-293E Cells. Lausanne, EPFL, 2013. DOI : 10.5075/epfl-thesis-5498.
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F. Zagari; M. Jordan; M. Stettler; H. Broly; F. M. Wurm : Lactate metabolism shift in CHO cell culture: the role of mitochondrial oxidative activity; New Biotechnology. 2013. DOI : 10.1016/j.nbt.2012.05.021.
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Z. Kadlecova; L. Baldi; D. Hacker; F. M. Wurm; H.-A. Klok : Comparative Study on the In Vitro Cytotoxicity of Linear, Dendritic, and Hyperbranched Polylysine Analogues; Biomacromolecules. 2012. DOI : 10.1021/bm300930j.
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P. O. Michel; C. Degen; M. Hubert; L. Baldi; D. L. Hacker et al. : A NanoDrop-based method for rapid determination of viability decline in suspension cultures of animal cells; Analytical Biochemistry. 2012. DOI : 10.1016/j.ab.2012.08.028.
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F. Zagari / F. Wurm; M. Jordan (Dir.) : Multidisciplinary Analysis of the Metabolic Shift to Lactate Consumption in CHO Cell Culture. Lausanne, EPFL, 2012. DOI : 10.5075/epfl-thesis-5420.
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W. Klöckner; S. Tissot; F. Wurm; J. Büchs : Power input correlation to characterize the hydrodynamics of cylindrical orbitally shaken bioreactors; Biochemical Engineering Journal. 2012. DOI : 10.1016/j.bej.2012.04.007.
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Y. Rajendra; D. Kiseljak; S. Manoli; L. Baldi; D. L. Hacker et al. : Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells; Biotechnology and Bioengineering. 2012. DOI : 10.1002/bit.24494.
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Z. Kadlecova; S. Nallet; D. L. Hacker; L. Baldi; H.-A. Klok et al. : Poly(ethyleneimine)-Mediated Large-Scale Transient Gene Expression: Influence of Molecular Weight, Polydispersity and N-Propionyl Groups; Macromolecular Bioscience. 2012. DOI : 10.1002/mabi.201100404.
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S. Tissot; P. O. Michel; D. L. Hacker; L. Baldi; M. D. Jesus et al. : k(L)a as a predictor for successful probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors; New biotechnology. 2012. DOI : 10.1016/j.nbt.2011.10.010.
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Y. Rajendra; D. Kiseljak; L. Baldi; D. L. Hacker; F. M. Wurm : Reduced glutamine concentration improves protein production in growth-arrested CHO-DG44 and HEK-293E cells; Biotechnology letters. 2012. DOI : 10.1007/s10529-011-0809-z.
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D. J. Capon; N. Kaneko; T. Yoshimori; T. Shimada; F. M. Wurm et al. : Flexible antibodies with nonprotein hinges; Proceedings Of The Japan Academy Series B-Physical And Biological Sciences. 2011. DOI : 10.2183/pjab.87.603.
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S. Tissot; M. Reclari; S. Quinodoz; M. Dreyer; D. T. Monteil et al. : Hydrodynamic stress in orbitally shaken bioreactors. 2011. p. P39. DOI : 10.1186/1753-6561-5-S8-P39.
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J. Pereira; Y. Rajendra; L. Baldi; D. L. Hacker; F. M. Wurm : Transient gene expression with CHO cells in conditioned medium: a study using TubeSpin® bioreactors. 2011. p. P38. DOI : 10.1186/1753-6561-5-S8-P38.
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X. Shen; P. O. Michel; Q. Xie; D. L. Hacker; F. M. Wurm : Transient transfection of insect Sf-9 cells in TubeSpin® bioreactor 50 tubes. 2011. p. P37. DOI : 10.1186/1753-6561-5-S8-P37.
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Y. Rajendra; D. Kiseljak; L. Baldi; D. L. Hacker; F. M. Wurm : Influence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells. 2011. p. P35. DOI : 10.1186/1753-6561-5-S8-P35.
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M. Matasci; V. Bachmann; L. Baldi; D. L. Hacker; M. De Jesus et al. : Rapid recombinant protein production from pools of transposon-generated CHO cells. 2011. p. P34. DOI : 10.1186/1753-6561-5-S8-P34.
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D. Kiseljak; Y. Rajendra; S. S. Manoli; L. Baldi; D. L. Hacker et al. : The use of filler DNA for improved transfection and reduced DNA needs in transient gene expression with CHO and HEK cells. 2011. p. P33. DOI : 10.1186/1753-6561-5-S8-P33.
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S. Balasubramanian; M. Matasci; L. Baldi; D. L. Hacker; F. M. Wurm : Transposon mediated co-integration and co-expression of transgenes in CHO-DG44 cells. 2011. p. P32. DOI : 10.1186/1753-6561-5-S8-P32.
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M. Matasci; V. Bachmann; L. Baldi; D. L. Hacker; M. De Jesus et al. : CHO cell lines generated by PiggyBac transposition. 2011. p. P31. DOI : 10.1186/1753-6561-5-S8-P31.
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Y. O. Tsybin; L. Fornelli; C. Stoermer; M. Luebeck; J. Parra et al. : Structural analysis of intact monoclonal antibodies by electron transfer dissociation mass spectrometry; Analytical chemistry. 2011. DOI : 10.1021/ac201293m.
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D. Favre : Mobile phone-induced honeybee worker piping; Apidologie. 2011. DOI : 10.1007/s13592-011-0016-x.
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L. Baldi; D. L. Hacker; C. Meerschman; F. M. Wurm : Large-Scale Transfection of Mammalian Cells; Methods in Molecular Biology -Clifton then Totowa-. 2011. DOI : 10.1007/978-1-61779-352-3_2.
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F. M. Wurm; D. Hacker : First CHO genome; Nature Biotechnology. 2011. DOI : 10.1038/nbt.1943.
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M. Matasci; L. Baldi; D. L. Hacker; F. M. Wurm : The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability; Biotechnology and Bioengineering. 2011. DOI : 10.1002/bit.23167.
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Y. Rajendra; D. Kiseljak; L. Baldi; D. L. Hacker; F. M. Wurm : A simple high-yielding process for transient gene expression in CHO cells; Journal of Biotechnology. 2011. DOI : 10.1016/j.jbiotec.2011.03.001.
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S. Tissot / F. Wurm (Dir.) : OrbShake Bioreactors for Mammalian Cell Cultures. Lausanne, EPFL, 2011. DOI : 10.5075/epfl-thesis-5135.
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S. Tissot; A. Oberbek; M. Reclari; M. Dreyer; D. L. Hacker et al. : Efficient and reproducible mammalian cell bioprocesses without probes and controllers?; New biotechnology. 2011. DOI : 10.1016/j.nbt.2011.02.004.
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M. De Jesus; F. M. Wurm : Manufacturing recombinant proteins in kg-ton quantities using animal cells in bioreactors; European Journal of Pharmaceutics and Biopharmaceutics. 2011. DOI : 10.1016/j.ejpb.2011.01.005.
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Q. Xie; P. O. Michel; L. Baldi; D. L. Hacker; X. Zhang et al. : TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension; Biotechnology Letters. 2011. DOI : 10.1007/s10529-011-0527-6.
Enseignement & Phd
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Oberbek Agata ...
Rajendra Yashas ...
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