Florian Maria Wurm

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Professeur honoraire

florian.wurm@epfl.ch +41 21 693 71 08 http://archiveweb.epfl.ch/lbtc.epfl.ch/

EPFL SV SV-DEC PH-SV
AAB 1 46 (Bâtiment AAB)
Station 19
CH-1015 Lausanne

+41 21 693 71 08
Local: AAB 1 46
EPFL > SV > SV-DEC > PH-SV

Web site: Site web: https://sv.epfl.ch/professeurs-honoraires

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Biographie

Florian Wurm a obtenu son doctorat en biologie moléculaire et travaux génétiques en 1979 à lUniversité de Giessen en Allemagne.

De 1980 à 1985, il dirigea un groupe de recherche au sein du Département de Virologie de Behringwerke, Marburg en Allemagne.

En 1985, il rejoint la Harvard Medical School à Boston dans le Massachussetts, en tant que hôte académique. De 1986 à 1995, il occupa les fonctions de « Scientist », « Senior Scientist », « Project-Team-Leader » puis « Senior Manager » à Genentech, Inc. à San Francisco, Californie.

En 1995, il est nommé professeur extraordinaire à lEcole Polytechnique Fédérale de Lausanne, puis professeur ordinaire en 1998.

Le professeur Wurm est consultant et membre de plusieurs sociétés scientifiques nationales et internationales, dont ESACT (European Society of Animal Cell Technology).

Il a récemment fondé une start-up en biotechnologie à Monthey en Suisse (ExcellGene SA).

Les recherches quil poursuit actuellement au sein de son laboratoire à lEPFL sont centrées sur le développement de technologies pour lexpression de protéines recombinantes à partir de cellules animales, et plus particulièrement de mammifères.

Publications

Publications Infoscience

Toutes les publications

* Recombinant Proteins for Industrial versus Pharmaceutical Purposes: A Review of Process and Pricing

J. Puetz; F. M. Wurm

Processes. 2019-08-01. DOI : 10.3390/pr7080476.

Florian Maria Wurm

Professeur honoraire

Biographie

Publications

Publications Infoscience

Toutes les publications

* Recombinant Proteins for Industrial versus Pharmaceutical Purposes: A Review of Process and Pricing

* Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells

* Contactless Respiration Monitoring in Real-Time via a Video Camera

* Improved process conditions for increasing expression of MHC class II protein from a stable Drosophila S2 cell line

* Fluid dynamics of flow fields in a disposable 600-mL orbitally shaken bioreactor

* Cloning of CHO Cells, Productivity and Genetic Stability—A Discussion

* Validation of Scalable and Compliant AAV Production

* Studies on fluid dynamics of the flow field and gas transfer in orbitally shaken tubes

* Multigene expression in stable CHO cell pools generated with the piggyBac transposon system

* A comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation

* Recombinant protein production from stable mammalian cell lines and pools

* Suspension-Adapted HEK 293 Cells in Orbital Shaken Bioreactors for the Production of Adeno-Associated Virus Vectors

* Comparison of Three Transposons for the Generation of Highly Productive Recombinant CHO Cell Pools and Cell Lines

* Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension

* Enhanced Plasmid DNA Utilization in Transiently Transfected CHO-DG44 Cells in the Presence of Polar Solvents

* A simple plasmid-based transient gene expression method using High Five cells

* Production of active glycosylation-deficient gamma-secretase complex for crystallization studies

* Transient Production of Recombinant Adeno-Associated Virus (AAV) Vectors for Gene Therapy Applications Using Suspension-Adapted HEK 293 Cells in Orbital Shaken Bioreactors

* Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools

* Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells

* Study of transposon-mediated cell pool and cell line generation in CHO cells

* Development of plasmid-based expression technology for rapid and sustained production of recombinant proteins in insect cells

* Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells

* The Engineering of a Scalable Disposable Orbitally Shaken Bioreactor System for Animal Cell Cultivation and Therapeutic Protein Production

* Surface wave dynamics in orbital shaken cylindrical containers

* Virus-free transient protein production in Sf9 cells

* CHO Quasispecies—Implications for Manufacturing Processes

* The optimization of a rapid low-cost alternative of large-scale medium sterilization

* Study of the improved Sf9 transient gene expression process

* Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells

* High expression of the aspartate–glutamate carrier Aralar1 favors lactate consumption in CHO cell culture

* Disposable 600-mL orbitally shaken bioreactor for mammalian cell cultivation in suspension

* Numerical simulation of orbitally shaken viscous fluids with free surface

* Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures

* DNA delivery with hyperbranched polylysine: A comparative study with linear and dendritic polylysine

* Highly efficient production of the Alzheimer's γ-Secretase integral membrane protease complex by a multi-gene stable integration approach

* A Simple High Yielding Transient Gene Expression System for CHO Cells

* The Use of Valproic Acid in Transient Gene Expression with HEK-293E Cells

* Lactate metabolism shift in CHO cell culture: the role of mitochondrial oxidative activity

* Comparative Study on the In Vitro Cytotoxicity of Linear, Dendritic, and Hyperbranched Polylysine Analogues

* A NanoDrop-based method for rapid determination of viability decline in suspension cultures of animal cells

* Multidisciplinary Analysis of the Metabolic Shift to Lactate Consumption in CHO Cell Culture

* Power input correlation to characterize the hydrodynamics of cylindrical orbitally shaken bioreactors

* Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells

* Poly(ethyleneimine)-Mediated Large-Scale Transient Gene Expression: Influence of Molecular Weight, Polydispersity and N-Propionyl Groups

* k(L)a as a predictor for successful probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors

* Reduced glutamine concentration improves protein production in growth-arrested CHO-DG44 and HEK-293E cells

* Flexible antibodies with nonprotein hinges

* Hydrodynamic stress in orbitally shaken bioreactors

* Transient gene expression with CHO cells in conditioned medium: a study using TubeSpin® bioreactors

* Transient transfection of insect Sf-9 cells in TubeSpin® bioreactor 50 tubes

* Influence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells

* Rapid recombinant protein production from pools of transposon-generated CHO cells

* The use of filler DNA for improved transfection and reduced DNA needs in transient gene expression with CHO and HEK cells

* Transposon mediated co-integration and co-expression of transgenes in CHO-DG44 cells

* CHO cell lines generated by PiggyBac transposition

* Structural analysis of intact monoclonal antibodies by electron transfer dissociation mass spectrometry

* Mobile phone-induced honeybee worker piping

* Large-Scale Transfection of Mammalian Cells

* First CHO genome

* The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability

* A simple high-yielding process for transient gene expression in CHO cells

* OrbShake Bioreactors for Mammalian Cell Cultures

* Efficient and reproducible mammalian cell bioprocesses without probes and controllers?

* Manufacturing recombinant proteins in kg-ton quantities using animal cells in bioreactors

* TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension

* Role of polymer architecture on polycation induced cell death: systematic study on molecular mechanism

* Innovative, Non-stirred Bioreactors in Scales from Milliliters up to 1000 Liters for Suspension Cultures of Cells using Disposable Bags and Containers – A Swiss Contribution

* Generation of stable, high-producing cho cell lines by lentiviral vector-mediated gene transfer in serum-free suspension culture

* Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells

* Lentiviral Vector-Mediated Gene Delivery for the Generation of Chinese Hamster Ovary Cell Lines with Improved Productivity and Stability

* Hyperosmolarity enhances transient recombinant protein yield in Chinese hamster ovary cells

* Transient Gene Expression in HEK-293E Cells for Recombinant Protein Production

* Determination of a scale-up factor from mixing time studies in orbitally shaken bioreactors