Florian Maria Wurm
Professeur honoraire
florian.wurm@epfl.ch +41 21 693 71 08 http://archiveweb.epfl.ch/lbtc.epfl.ch/
EPFL SV SV-DEC PH-SV
AAB 1 46 (Bâtiment AAB)
Station 19
CH-1015 Lausanne
Web site: Site web: https://sv.epfl.ch/professeurs-honoraires
Biographie
Florian Wurm a obtenu son doctorat en biologie moléculaire et travaux génétiques en 1979 à lUniversité de Giessen en Allemagne.De 1980 à 1985, il dirigea un groupe de recherche au sein du Département de Virologie de Behringwerke, Marburg en Allemagne.
En 1985, il rejoint la Harvard Medical School à Boston dans le Massachussetts, en tant que hôte académique. De 1986 à 1995, il occupa les fonctions de « Scientist », « Senior Scientist », « Project-Team-Leader » puis « Senior Manager » à Genentech, Inc. à San Francisco, Californie.
En 1995, il est nommé professeur extraordinaire à lEcole Polytechnique Fédérale de Lausanne, puis professeur ordinaire en 1998.
Le professeur Wurm est consultant et membre de plusieurs sociétés scientifiques nationales et internationales, dont ESACT (European Society of Animal Cell Technology).
Il a récemment fondé une start-up en biotechnologie à Monthey en Suisse (ExcellGene SA).
Les recherches quil poursuit actuellement au sein de son laboratoire à lEPFL sont centrées sur le développement de technologies pour lexpression de protéines recombinantes à partir de cellules animales, et plus particulièrement de mammifères.
Publications
Publications Infoscience
Toutes les publications
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J. Puetz; F. M. Wurm : Recombinant Proteins for Industrial versus Pharmaceutical Purposes: A Review of Process and Pricing; Processes. 2019-08-01. DOI : 10.3390/pr7080476.
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X. Shen; D. Dojcinovic; L. Baldi; D. Hacker; I. Luescher et al. : Improved process conditions for increasing expression of MHC class II protein from a stable Drosophila S2 cell line; BIOTECHNOLOGY LETTERS. 2018. DOI : 10.1007/s10529-017-2440-0.
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L. Zhu; D. T. Monteil; Y. Wang; B. Song; D. L. Hacker et al. : Fluid dynamics of flow fields in a disposable 600-mL orbitally shaken bioreactor; Biochemical Engineering Journal. 2018. DOI : 10.1016/j.bej.2017.10.019.
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F. M. Wurm; M. J. Wurm : Cloning of CHO Cells, Productivity and Genetic Stability-A Discussion; Processes. 2017. DOI : 10.3390/pr5020020.
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D. R. Blessing; G. Vachey; B. Schneider; F. M. Wurm; N. Deglon : Validation of Scalable and Compliant AAV Production. 2017. 20th Annual Meeting of the American-Society-of-Gene-and-Cell-Therapy (ASGCT), Washington, DC, MAY 10-13, 2017. p. 326-326.
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L.-K. Zhu; B.-Y. Song; Z.-L. Wang; D. T. Monteil; X. Shen et al. : Studies on fluid dynamics of the flow field and gas transfer in orbitally shaken tubes; Biotechnology Progress. 2017. DOI : 10.1002/btpr.2375.
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S. Balasubramanian; F. M. Wurm; D. L. Hacker : Multigene expression in stable CHO cell pools generated with the piggyBac transposon system; Biotechnology Progress. 2016. DOI : 10.1002/btpr.2319.
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D. T. Monteil; V. Juvet; J. Paz; M. Moniatte; L. Baldi et al. : A comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation; Biotechnology Progress. 2016. DOI : 10.1002/btpr.2328.
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D. L. Hacker; S. Balasubramanian : Recombinant protein production from stable mammalian cell lines and pools; Current Opinion In Structural Biology. 2016. DOI : 10.1016/j.sbi.2016.06.005.
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D. R. Blessing; B. Schneider; F. M. Wurm; N. Deglon : Suspension-Adapted HEK 293 Cells in Orbital Shaken Bioreactors for the Production of Adeno-Associated Virus Vectors. 2016. 19th Annual Meeting of the American-Society-of-Gene-and-Cell-Therapy (ASGCT), Washington DC, USA, May 4-7, 2016. p. S183-S184.
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S. Balasubramanian; Y. Rajendra; L. Baldi; D. L. Hacker; F. M. Wurm : Comparison of Three Transposons for the Generation of Highly Productive Recombinant CHO Cell Pools and Cell Lines; Biotechnology And Bioengineering. 2016. DOI : 10.1002/bit.25888.
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D. T. Monteil; X. Shen; G. Tontodonati; L. Baldi; D. L. Hacker et al. : Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension; Analytical Biochemistry. 2016. DOI : 10.1016/j.ab.2016.04.009.
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Y. Rajendra; S. Balasubramanian; D. Kiseljak; L. Baldi; F. M. Wurm et al. : Enhanced Plasmid DNA Utilization in Transiently Transfected CHO-DG44 Cells in the Presence of Polar Solvents; Biotechnology Progress. 2015. DOI : 10.1002/btpr.2152.
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X. Shen; A. K. Pitol; V. Bachmann; D. L. Hacker; L. Baldi et al. : A simple plasmid-based transient gene expression method using High Five cells; Journal Of Biotechnology. 2015. DOI : 10.1016/j.jbiotec.2015.10.007.
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A. R. Lopez; M. Dimitrov; H. Gerber; V. Braman; D. L. Hacker et al. : Production of active glycosylation-deficient gamma-secretase complex for crystallization studies; Biotechnology And Bioengineering. 2015. DOI : 10.1002/bit.25675.
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D. R. Blessing; M. Montavon; M. De Jesus; L. Baldi; B. Schneider et al. : Transient Production of Recombinant Adeno-Associated Virus (AAV) Vectors for Gene Therapy Applications Using Suspension-Adapted HEK 293 Cells in Orbital Shaken Bioreactors. 2015. 18th Annual Meeting of the American-Society-of-Gene-and-Cell-Therapy (ASGCT)'. p. S184-S185.
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S. Balasubramanian; M. Matasci; Z. Kadlecova; L. Baldi; D. L. Hacker et al. : Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools; Journal of Biotechnology. 2015. DOI : 10.1016/j.jbiotec.2015.03.001.
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Y. Rajendra; D. Kiseljak; L. Baldi; F. M. Wurm; D. L. Hacker : Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells; Biotechnology Progress. 2015. DOI : 10.1002/btpr.2064.
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S. Balasubramanian / F. M. Wurm; D. Hacker (Dir.) : Study of transposon-mediated cell pool and cell line generation in CHO cells. Lausanne, EPFL, 2015. DOI : 10.5075/epfl-thesis-6563.
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X. Shen / F. M. Wurm; D. Hacker (Dir.) : Development of plasmid-based expression technology for rapid and sustained production of recombinant proteins in insect cells. Lausanne, EPFL, 2014. DOI : 10.5075/epfl-thesis-6435.
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F. Davami; L. Baldi; Y. Rajendra; F. M Wurm : Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells; International journal of molecular and cellular medicine. 2014.
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D. T. Monteil Jr / F. M. Wurm; D. Hacker (Dir.) : The Engineering of a Scalable Disposable Orbitally Shaken Bioreactor System for Animal Cell Cultivation and Therapeutic Protein Production. Lausanne, EPFL, 2014. DOI : 10.5075/epfl-thesis-6423.
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M. Reclari; M. Dreyer; S. Tissot; D. Obreschkow; F. M. Wurm et al. : Surface wave dynamics in orbital shaken cylindrical containers; Physics of Fluids. 2014. DOI : 10.1063/1.4874612.
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X. Shen; D. L. Hacker; L. Baldi; F. M. Wurm : Virus-free transient protein production in Sf9 cells; Journal Of Biotechnology. 2014. DOI : 10.1016/j.jbiotec.2013.11.018.
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F. Wurm : CHO Quasispecies—Implications for Manufacturing Processes; Processes. 2013. DOI : 10.3390/pr1030296.
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D. T. Monteil; C. A. Bürki; L. Baldi; D. L. Hacker; M. De Jesus et al. : The optimization of a rapid low-cost alternative of large-scale medium sterilization. 2013. 23rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health, Lille, France, 23-26 June 2013. p. P45. DOI : 10.1186/1753-6561-7-S6-P45.
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X. Shen; D. L. Hacker; L. Baldi; F. M. Wurm : Study of the improved Sf9 transient gene expression process. 2013. 23rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health, Lille, France, 23-26 June 2013. p. P19. DOI : 10.1186/1753-6561-7-S6-P19.
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D. L. Hacker; D. Kiseljak; Y. Rajendra; S. Thurnheer; L. Baldi et al. : Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells; Protein Expression and Purification. 2013. DOI : 10.1016/j.pep.2013.09.001.
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F. Zagari; M. Stettler; L. Baldi; H. Broly; F. M. Wurm et al. : High expression of the aspartate–glutamate carrier Aralar1 favors lactate consumption in CHO cell culture; Pharmaceutical Bioprocessing. 2013. DOI : 10.4155/pbp.13.5.
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D. T. Monteil; G. Tontodonati; S. Ghimire; L. Baldi; D. L. Hacker et al. : Disposable 600-mL orbitally shaken bioreactor for mammalian cell cultivation in suspension; Biochemical Engineering Journal. 2013. DOI : 10.1016/j.bej.2013.04.008.
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M. Discacciati; D. Hacker; A. Quarteroni; S. Quinodoz; S. Tissot et al. : Numerical simulation of orbitally shaken viscous fluids with free surface; International Journal For Numerical Methods In Fluids. 2013. DOI : 10.1002/fld.3658.
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S. Tissot; P. Michel; C. Douet; S. Grezet; L. Baldi et al. : Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures; Processes. 2013. DOI : 10.3390/pr1010003.
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Z. Kadlecova; Y. Rajendra; M. Matasci; L. Baldi; D. L. Hacker et al. : DNA delivery with hyperbranched polylysine: A comparative study with linear and dendritic polylysine; Journal of Controlled Release. 2013. DOI : 10.1016/j.jconrel.2013.01.019.
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J.-R. Alattia; M. Matasci; M. Dimitrov; L. Aeschbach; S. Balasubramanian et al. : Highly efficient production of the Alzheimer's γ-Secretase integral membrane protease complex by a multi-gene stable integration approach; Biotechnology and Bioengineering. 2013. DOI : 10.1002/bit.24851.
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Y. Rajendra / F. M. Wurm; D. Hacker (Dir.) : A Simple High Yielding Transient Gene Expression System for CHO Cells. Lausanne, EPFL, 2013. DOI : 10.5075/epfl-thesis-5572.
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D. Kiseljak / F. M. Wurm (Dir.) : The Use of Valproic Acid in Transient Gene Expression with HEK-293E Cells. Lausanne, EPFL, 2013. DOI : 10.5075/epfl-thesis-5498.
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F. Zagari; M. Jordan; M. Stettler; H. Broly; F. M. Wurm : Lactate metabolism shift in CHO cell culture: the role of mitochondrial oxidative activity; New Biotechnology. 2013. DOI : 10.1016/j.nbt.2012.05.021.
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Z. Kadlecova; L. Baldi; D. Hacker; F. M. Wurm; H.-A. Klok : Comparative Study on the In Vitro Cytotoxicity of Linear, Dendritic, and Hyperbranched Polylysine Analogues; Biomacromolecules. 2012. DOI : 10.1021/bm300930j.
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P. O. Michel; C. Degen; M. Hubert; L. Baldi; D. L. Hacker et al. : A NanoDrop-based method for rapid determination of viability decline in suspension cultures of animal cells; Analytical Biochemistry. 2012. DOI : 10.1016/j.ab.2012.08.028.
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F. Zagari / F. Wurm; M. Jordan (Dir.) : Multidisciplinary Analysis of the Metabolic Shift to Lactate Consumption in CHO Cell Culture. Lausanne, EPFL, 2012. DOI : 10.5075/epfl-thesis-5420.
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W. Klöckner; S. Tissot; F. Wurm; J. Büchs : Power input correlation to characterize the hydrodynamics of cylindrical orbitally shaken bioreactors; Biochemical Engineering Journal. 2012. DOI : 10.1016/j.bej.2012.04.007.
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Y. Rajendra; D. Kiseljak; S. Manoli; L. Baldi; D. L. Hacker et al. : Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells; Biotechnology and Bioengineering. 2012. DOI : 10.1002/bit.24494.
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Z. Kadlecova; S. Nallet; D. L. Hacker; L. Baldi; H.-A. Klok et al. : Poly(ethyleneimine)-Mediated Large-Scale Transient Gene Expression: Influence of Molecular Weight, Polydispersity and N-Propionyl Groups; Macromolecular Bioscience. 2012. DOI : 10.1002/mabi.201100404.
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S. Tissot; P. O. Michel; D. L. Hacker; L. Baldi; M. D. Jesus et al. : k(L)a as a predictor for successful probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors; New biotechnology. 2012. DOI : 10.1016/j.nbt.2011.10.010.
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Y. Rajendra; D. Kiseljak; L. Baldi; D. L. Hacker; F. M. Wurm : Reduced glutamine concentration improves protein production in growth-arrested CHO-DG44 and HEK-293E cells; Biotechnology letters. 2012. DOI : 10.1007/s10529-011-0809-z.
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D. J. Capon; N. Kaneko; T. Yoshimori; T. Shimada; F. M. Wurm et al. : Flexible antibodies with nonprotein hinges; Proceedings Of The Japan Academy Series B-Physical And Biological Sciences. 2011. DOI : 10.2183/pjab.87.603.
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S. Tissot; M. Reclari; S. Quinodoz; M. Dreyer; D. T. Monteil et al. : Hydrodynamic stress in orbitally shaken bioreactors. 2011. p. P39. DOI : 10.1186/1753-6561-5-S8-P39.
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J. Pereira; Y. Rajendra; L. Baldi; D. L. Hacker; F. M. Wurm : Transient gene expression with CHO cells in conditioned medium: a study using TubeSpin® bioreactors. 2011. p. P38. DOI : 10.1186/1753-6561-5-S8-P38.
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X. Shen; P. O. Michel; Q. Xie; D. L. Hacker; F. M. Wurm : Transient transfection of insect Sf-9 cells in TubeSpin® bioreactor 50 tubes. 2011. p. P37. DOI : 10.1186/1753-6561-5-S8-P37.
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Y. Rajendra; D. Kiseljak; L. Baldi; D. L. Hacker; F. M. Wurm : Influence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells. 2011. p. P35. DOI : 10.1186/1753-6561-5-S8-P35.
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M. Matasci; V. Bachmann; L. Baldi; D. L. Hacker; M. De Jesus et al. : Rapid recombinant protein production from pools of transposon-generated CHO cells. 2011. p. P34. DOI : 10.1186/1753-6561-5-S8-P34.
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D. Kiseljak; Y. Rajendra; S. S. Manoli; L. Baldi; D. L. Hacker et al. : The use of filler DNA for improved transfection and reduced DNA needs in transient gene expression with CHO and HEK cells. 2011. p. P33. DOI : 10.1186/1753-6561-5-S8-P33.
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S. Balasubramanian; M. Matasci; L. Baldi; D. L. Hacker; F. M. Wurm : Transposon mediated co-integration and co-expression of transgenes in CHO-DG44 cells. 2011. p. P32. DOI : 10.1186/1753-6561-5-S8-P32.
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M. Matasci; V. Bachmann; L. Baldi; D. L. Hacker; M. De Jesus et al. : CHO cell lines generated by PiggyBac transposition. 2011. p. P31. DOI : 10.1186/1753-6561-5-S8-P31.
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Y. O. Tsybin; L. Fornelli; C. Stoermer; M. Luebeck; J. Parra et al. : Structural analysis of intact monoclonal antibodies by electron transfer dissociation mass spectrometry; Analytical chemistry. 2011. DOI : 10.1021/ac201293m.
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D. Favre : Mobile phone-induced honeybee worker piping; Apidologie. 2011. DOI : 10.1007/s13592-011-0016-x.
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L. Baldi; D. L. Hacker; C. Meerschman; F. M. Wurm : Large-Scale Transfection of Mammalian Cells; Methods in Molecular Biology -Clifton then Totowa-. 2011. DOI : 10.1007/978-1-61779-352-3_2.
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F. M. Wurm; D. Hacker : First CHO genome; Nature Biotechnology. 2011. DOI : 10.1038/nbt.1943.
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M. Matasci; L. Baldi; D. L. Hacker; F. M. Wurm : The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability; Biotechnology and Bioengineering. 2011. DOI : 10.1002/bit.23167.
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Y. Rajendra; D. Kiseljak; L. Baldi; D. L. Hacker; F. M. Wurm : A simple high-yielding process for transient gene expression in CHO cells; Journal of Biotechnology. 2011. DOI : 10.1016/j.jbiotec.2011.03.001.
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S. Tissot / F. Wurm (Dir.) : OrbShake Bioreactors for Mammalian Cell Cultures. Lausanne, EPFL, 2011. DOI : 10.5075/epfl-thesis-5135.
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S. Tissot; A. Oberbek; M. Reclari; M. Dreyer; D. L. Hacker et al. : Efficient and reproducible mammalian cell bioprocesses without probes and controllers?; New biotechnology. 2011. DOI : 10.1016/j.nbt.2011.02.004.
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M. De Jesus; F. M. Wurm : Manufacturing recombinant proteins in kg-ton quantities using animal cells in bioreactors; European Journal of Pharmaceutics and Biopharmaceutics. 2011. DOI : 10.1016/j.ejpb.2011.01.005.
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Q. Xie; P. O. Michel; L. Baldi; D. L. Hacker; X. Zhang et al. : TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension; Biotechnology Letters. 2011. DOI : 10.1007/s10529-011-0527-6.
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Z. Kadlecova; E. Corbacella; F. M. Wurm; H. A. Klok : Role of polymer architecture on polycation induced cell death: systematic study on molecular mechanism. 2010. 3rd International Cellular Delivery of Therapeutic Macromolecules (CDTM) Symposium, Cardiff, ENGLAND, Jun 26-29, 2010. p. 1086-1086. DOI : 10.1016/j.drudis.2010.09.369.
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S. Werner; R. Eibl; C. Lettenbauer; M. Röll; D. Eibl et al. : Innovative, Non-stirred Bioreactors in Scales from Milliliters up to 1000 Liters for Suspension Cultures of Cells using Disposable Bags and Containers – A Swiss Contribution; CHIMIA International Journal for Chemistry. 2010. DOI : 10.2533/chimia.2010.819.
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A. Oberbek; M. Matasci; D. L. Hacker; F. M. Wurm : Generation of stable, high-producing cho cell lines by lentiviral vector-mediated gene transfer in serum-free suspension culture; Biotechnology and Bioengineering. 2010. DOI : 10.1002/bit.22968.
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S. Wulhfard; L. Baldi; D. L. Hacker; F. Wurm : Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells; Journal of biotechnology. 2010. DOI : 10.1016/j.jbiotec.2010.05.003.
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A. Oberbek / F. Wurm; D. Hacker (Dir.) : Lentiviral Vector-Mediated Gene Delivery for the Generation of Chinese Hamster Ovary Cell Lines with Improved Productivity and Stability. Lausanne, EPFL, 2010. DOI : 10.5075/epfl-thesis-4834.
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X. Zhang; I. F. Garcia; L. Baldi; D. L. Hacker; F. M. Wurm : Hyperosmolarity enhances transient recombinant protein yield in Chinese hamster ovary cells; Biotechnology Letters. 2010. DOI : 10.1007/s10529-010-0331-8.
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S. Nallet / F. Wurm (Dir.) : Transient Gene Expression in HEK-293E Cells for Recombinant Protein Production. Lausanne, EPFL, 2010. DOI : 10.5075/epfl-thesis-4815.
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S. Tissot; M. Farhat; D. L. Hacker; T. Anderlei; M. Kühner et al. : Determination of a scale-up factor from mixing time studies in orbitally shaken bioreactors; Biochemical Engineering Journal. 2010. DOI : 10.1016/j.bej.2010.08.005.
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E.-M. Engelhardt; E. Stegberg; R. A. Brown; J. A. Hubbell; F. M. Wurm et al. : Compressed collagen gel: a novel scaffold for human bladder cells; Journal of Tissue Engineering and Regenerative Medicine. 2010. DOI : 10.1002/term.222.
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A. Bello; K. Tran; A. Chand; M. Doria; M. Allocca et al. : Isolation and evaluation of novel adeno-associated virus sequences from porcine tissues; Gene Therapy. 2009. DOI : 10.1038/gt.2009.82.
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D. L. Hacker; L. Baldi; M. Adam; F. M. Wurm : Transient Gene Expression in Mammalian Cells: Promises and Challenges for Medical Biotechnology; Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology; Hoboken, N.J: Wiley, 2009.
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X. Zhang / F. Wurm (Dir.) : Orbitally shaken bioreactors for mammalian cell culture. Lausanne, EPFL, 2009. DOI : 10.5075/epfl-thesis-4492.
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S. Chenuet; M. Derouazi; D. Hacker; F. Wurm : DNA delivery by microinjection for the generation of recombinant mammalian cell lines; Microinjection: Methods and Applications; Humana Press, 2009. p. 99-112.
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D. L. Hacker; M. De Jesus; F. M. Wurm : 25 years of recombinant proteins from reactor-grown cells — Where do we go from here?; Biotechnology Advances. 2009. DOI : 10.1016/j.biotechadv.2009.05.008.
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X. Zhang; M. Stettler; D. Sanctis; M. Perrone; N. Parolini et al. : Use of Orbital Shaken Disposable Bioreactors for Mammalian Cell Cultures from the Milliliter-Scale to the 1,000-Liter Scale; Advances in Biochemical Engineering / Biotechnology. 2009. DOI : 10.1007/10_2008_18.
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S. Nallet; M. Amacker; N. Westerfeld; L. Baldi; I. König et al. : Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells; Vaccine. 2009. DOI : 10.1016/j.vaccine.2009.06.019.
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E.-M. Engelhardt / F. Wurm; P. Frey (Dir.) : Studies on scaffold design for tissue engineering applications. Lausanne, EPFL, 2009. DOI : 10.5075/epfl-thesis-4445.
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E. Engelhardt; S. Houis; T. Gries; J. Hilborn; M. Adam et al. : Suspension-adapted Chinese hamster ovary-derived cells expressing green fluorescent protein as a screening tool for biomaterials; Biotechnol Lett. 2009. DOI : 10.1007/s10529-009-9997-1.
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S. Wulhfard / F. Wurm (Dir.) : Transient recombinant protein expression in mammalian cells. Lausanne, EPFL, 2009. DOI : 10.5075/epfl-thesis-4393.
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M. B. Irving; M. Andre; V. Zoete; D. A. Schmid; P. Reichenbach et al. : Production and characterisation of rationally optimised, anti-melanoma T-cell receptors: a step closer towards immunotherapeutic treatment for patients. 2008. Annual joint meeting of the Swiss Societies for Pneumology, Paediatric Pneumology, Allergology and Immunology, Thoracic Surgery, Fribourg, April 17-18, 2008. p. 44S.
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E.-M. Engelhardt; S. Houis; R. A. Brown; J. Hillborn; M. Adam et al. : Recombinant human collagen as a bioactive element in scaffolds for tissue engineering ; Annual Tissue-Engineering-and-Regenerative-Medicine-International-Society-European-Chapter Meeting, Porto, PORTUGAL, Jun, 2008.
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E.-M. Engelhardt; M. Ananta; F. Foroughi; V. Mudera; R. A. Brown et al. : Compressed collagen-PLA hybrids as scaffold for human bladder cells ; Annual Tissue-Engineering-and-Regenerative-Medicine-International-Society-European-Chapter Meeting, Porto, PORTUGAL, Jun, 2008.
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M. Bertschinger; A. Schertenleib; J. Cevey; D. L. Hacker; F. M. Wurm : The kinetics of polyethylenimine-mediated transfection in suspension cultures of Chinese hamster ovary cells; Molecular Biotechnology. 2008. DOI : 10.1007/s12033-008-9069-0.
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M. Matasci; D. L. Hacker; L. Baldi; F. M. Wurm : Recombinant therapeutic protein production in cultivated mammalian cells: current status and future prospects; Drug Discovery Today: Technologies. 2008. DOI : 10.1016/j.ddtec.2008.12.003.
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S. Wulhfard; S. Tissot; S. Bouchet; J. Cevey; M. De Jesus et al. : Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells; BIOTECHNOL PROG.. 2008.
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G. Backliwal; M. Hildinger; I. Kuettel; F. Delegrange; D. L. Hacker et al. : Valproic acid: A viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures; Biotechnology and Bioengineering. 2008. DOI : 10.1002/bit.21882.
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X. Zhang; M. Stettler; O. Reif; A. Kocourek; M. Dejesus et al. : Shaken helical track bioreactors: Providing oxygen to high-density cultures of mammalian cells at volumes up to 1000L by surface aeration with air; New Biotechnology. 2008. DOI : 10.1016/j.nbt.2008.03.001.
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G. Backliwal; M. Hildinger; S. Chenuet; S. Wulhfard; M. De Jesus et al. : Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions; NUCLEIC ACIDS RES.. 2008. DOI : 10.1093/nar/gkn423.
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S. Chenuet; D. Martinet; N. Besuchet-Schmutz; M. Wicht; N. Jaccard et al. : Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection; Biotechnology and Bioengineering. 2008. DOI : 10.1002/bit.21972.
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G. Backliwal; M. Hildinger; S. Chenuet; M. Dejesus; F. Wurm : Coexpression of acidic fibroblast growth factor enhances specific productivity and antibody titers in transiently transfected HEK293 cells; New Biotechnology. 2008. DOI : 10.1016/j.nbt.2008.08.007.
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G. Backliwal / F. Wurm (Dir.) : Development of high-titer transient gene expression processes for manufacturing recombinant proteins. Lausanne, EPFL, 2008. DOI : 10.5075/epfl-thesis-4174.
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S. Chenuet / F. Wurm (Dir.) : Accelerating the development time of recombinant mammalian cell lines producing high titers of protein therapeutics. Lausanne, EPFL, 2008. DOI : 10.5075/epfl-thesis-4128.
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K. Stirnemann; J. F. Romero; L. Baldi; B. Robert; V. Cesson et al. : Sustained activation and tumor targeting of NKT cells using a CD1d-anti-HER2-scFv fusion protein induce antitumor effects in mice; Journal of Clinical Investigation. 2008. DOI : 10.1172/JCI33249.
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M. Hildinger; L. Baldi; M. Stettler; F. M. Wurm : High-titer, serum-free production of adeno-associated virus vectors by polyethyleneimine-mediated plasmid transfection in mammalian suspension cells; Biotechnology Letters. 2007. DOI : 10.1007/s10529-007-9441-3.
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F. M. Wurm : Manufacturing of Biopharmaceuticals and Implications for Biosimilars; Kidney and Blood Pressure Research. 2007. DOI : 10.1159/000107094.
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M. Stettler; X. Zhang; D. L. Hacker; M. De Jesus; F. M. Wurm : Novel orbital shake bioreactors for transient production of CHO derived IgGs; Biotechnology Progress. 2007. DOI : 10.1021/bp070219i.
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G. Backliwal; M. Hildinger; V. Hasija; F. M. Wurm : High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI; Biotechnology and Bioengineering. 2007. DOI : 10.1002/bit.21596.
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M. Stettler / F. Wurm (Dir.) : Bioreactor processes based on disposable materials for the production of recombinant proteins from mammalian cells. Lausanne, EPFL, 2007. DOI : 10.5075/epfl-thesis-3947.
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N. Muller; M. Derouazi; F. Van Tilborgh; S. Wulhfard; D. L. Hacker et al. : Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems; Biotechnol Lett. 2007. DOI : 10.1007/s10529-006-9298-x.
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L. Baldi; D. Hacker; M. Adam; F. Wurm : Recombinant protein production by large-scale transient gene expression in mammalian cells : state of the art and future perspectives; Biotechnology Letters. 2007. DOI : 10.1007/s10529-006-9297-y.
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K. Ehrmann; N. Saillen; F. Vincent; M. Stettler; M. Jordan et al. : Microfabricated solenoids and Helmholtz coils for NMR spectroscopy of mammalian cells; Lab on a chip. 2007. DOI : 10.1039/b614044k.
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K. Ehrmann; K. Pataky; M. Stettler; F. M. Wurm; J. Brugger et al. : NMR spectroscopy and perfusion of mammalian cells using surface microprobes; Lab on a Chip. 2007.
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M. Stettler; N. Jaccard; D. Hacker; M. De Jesus; F. M. Wurm et al. : New disposable tubes for rapid and precise biomass assessment for suspension cultures of mammalian cells; Biotechnol Bioeng. 2006. DOI : 10.1002/bit.21071.
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M. Bertschinger; G. Backliwal; A. Schertenleib; M. Jordan; D. L. Hacker et al. : Disassembly of polyethylenimine-DNA particles in vitro: implications for polyethylenimine-mediated DNA delivery; J Control Release. 2006. DOI : 10.1016/j.jconrel.2006.09.006.
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F. Grosjean; M. Bertschinger; D. L. Hacker; F. M. Wurm : Multiple glycerol shocks increase the calcium phosphate transfection of non-synchronized CHO cells; Biotechnology letters. 2006. DOI : 10.1007/s10529-006-9167-7.
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M. Derouazi; D. Martinet; N. Besuchet Schmutz; R. Flaction; M. Wicht et al. : Genetic characterization of CHO production host DG44 and derivative recombinant cell lines; Biochemical and biophysical research communications. 2006. DOI : 10.1016/j.bbrc.2005.12.111.
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M. Derouazi; R. Flaction; P. Girard; M. de Jesus; M. Jordan et al. : Generation of recombinant Chinese hamster ovary cell lines by microinjection; Biotechnology letters. 2006. DOI : 10.1007/s10529-005-6062-6.
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M. Bertschinger; C. Burki; G. Backliwal; D. L. Hacker; M. Jordan et al. : Polyethylenimine-based quality control assay for plasmid DNA; Analytical biochemistry. 2006. DOI : 10.1016/j.ab.2006.05.002.
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M. Bertschinger / F. Wurm (Dir.) : The use of RNA interference to increase PEI-mediated transient gene expression in mammalian cells. Lausanne, EPFL, 2006. DOI : 10.5075/epfl-thesis-3450.
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L. Hunt; D. L. Hacker; F. Grosjean; M. De Jesus; L. Uebersax et al. : Low-temperature pausing of cultivated mammalian cells; Biotechnol Bioeng. 2005. DOI : 10.1002/bit.20320.
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F. Wurm; D. Hacker; M. Jordan : Mammalian cell culture productivity at large scale – from grams/liter to tens of grams/liter soon?. 2005. p. 509-512.
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W. Weber; L. Malphettes; M. de Jesus; R. Schoenmakers; M. D. El-Baba et al. : Engineered Streptomyces quorum-sensing components enable inducible siRNA-mediated translation control in mammalian cells and adjustable transcription control in mice; journal of gene medicine. 2005. DOI : 10.1002/jgm.682.
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N. Muller; P. Girard; D. L. Hacker; M. Jordan; F. M. Wurm : Orbital shaker technology for the cultivation of mammalian cells in suspension; Biotechnology and bioengineering. 2005. DOI : 10.1002/bit.20358.
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M. C. Smales; S. Geisse; M. Jordan; F. M. Wurm : Large-scale transient expression of therapeutic proteins in mammalian cells; Therapeutic Proteins: Methods and Protocols; Totowa, New jersey: Humana Press, 2005. p. 87-98.
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D. L. Hacker; E. Derow; F. M. Wurm : The CELO adenovirus Gam1 protein enhances transient and stable recombinant protein expression in Chinese hamster ovary cells; Journal of biotechnology. 2005. DOI : 10.1016/j.jbiotec.2005.01.006.
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L. Baldi; N. Muller; S. Picasso; R. Jacquet; P. Girard et al. : Transient gene expression in suspension HEK-293 cells: application to large-scale protein production; Biotechnology progress. 2005. DOI : 10.1021/bp049830x.
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N. Muller / F. Wurm (Dir.) : Transient gene expression for rapid protein production. Lausanne, EPFL, 2005. DOI : 10.5075/epfl-thesis-3297.
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M. Derouazi / F. Wurm (Dir.) : Establishment of recombinant mammalian cell lines. Lausanne, EPFL, 2005. DOI : 10.5075/epfl-thesis-3150.
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J. M. Pachlopnik; M. P. Horn; M. Fux; M. Dahinden; M. Mandallaz et al. : Natural anti-FcepsilonRIalpha autoantibodies may interfere with diagnostic tests for autoimmune urticaria; J Autoimmun. 2004. DOI : 10.1016/j.jaut.2003.09.007.
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D. Saverino; F. Ghiotto; A. Merlo; S. Bruno; L. Battini et al. : Specific recognition of the viral protein UL18 by CD85j/LIR-1/ILT2 on CD8+ T cells mediates the non-MHC-restricted lysis of human cytomegalovirus-infected cells; Journal of Immunology. 2004.
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M. De Jesus; P. Girard; M. Bourgeois; M. Baumgartner; G. Jacko et al. : Tubespin satellites: a fast track approach for process development with animal cells using shaking technology; Biochemical Engineering Journal. 2004. DOI : 10.1016/S1369-703X(03)00180-3.
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J. Lindell; P. Girard; N. Müller; M. Jordan; F. Wurm : Calfection – a novel transfection method for mammalian cells cultivated in suspensio; Biochemica Biophysica Acta – Gene Structure and Expression. 2004.
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F. M. Wurm : Production of recombinant protein therapeutics in cultivated mammalian cells; Nature biotechnology. 2004. DOI : 10.1038/nbt1026.
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M. Jordan; F. Wurm : Transfection of adherent and suspended cells by calcium phosphate; Methods. 2004.
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D. L. Hacker; M. Bertschinger; L. Baldi; F. M. Wurm : Reduction of adenovirus E1A mRNA by RNAi results in enhanced recombinant protein expression in transiently transfected HEK293 cells; Gene. 2004. DOI : 10.1016/j.gene.2004.06.054.
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M. Derouazi; P. Girard; F. Van Tilborgh; K. Iglesias; N. Muller et al. : Serum-free large-scale transient transfection of CHO cells; Biotechnology and bioengineering. 2004. DOI : 10.1002/bit.20161.
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M. Bertschinger; S. Chaboche; M. Jordan; F. M. Wurm : A spectrophotometric assay for the quantification of polyethylenimine in DNA nanoparticles; Analytical biochemistry. 2004. DOI : 10.1016/j.ab.2004.07.020.
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J. Lindell; P. Girard; N. Muller; M. Jordan; F. Wurm : Calfection: a novel gene transfer method for suspension cells; Biochimica et biophysica acta. 2004.
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P. Girard; M. Jordan; F. Wurm ; A method for introducing a nucleic acid into a eukaryotic cell. WO03000908 ; EP1270733 . 2003.
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L. Baldi; R. Jacquet; S. Picasso; P. Tromba; E. Derow et al. : Transient gene expression in suspension HEK-293 cells: application to large-scale protein production. 2003. p. 365-367.
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M. Derouazi; H. Pick; C. Deluz; S. Picasso; F. Wurm : Towards an affinity-based capture system for the isolation of high expression cells using a co-expressed surface protein. 2003. p. 471-473.
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S. C. Makrides; F. M. Wurm; M. Jordan : Gene transfer and gene amplification in mammalian cells; Gene Transfer and Expression in Mammalian Cells.; Amsterdam: Elsevier Science, 2003. p. 309-335.
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M. Jordan; F. Wurm : Co-transfer of multiple plasmids/viruses as an attractive method to introduce several genes in mammalian cells; New Comprehensive Biochemistry; 2003. p. 337-357.
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F. M. Wurm : Human therapeutic proteins from silkworms; Nature biotechnology. 2003. DOI : 10.1038/nbt0103-34.
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J. L. Wright; M. Jordan; F. M. Wurm : Transfection of partially purified plasmid DNA for high level transient protein expression in HEK293-EBNA cells; Journal of biotechnology. 2003. DOI : 10.1016/S0168-1656(03)00032-4.
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L. Hunt; D. Hacker; H. El Abridi; H. Grosjean; M. Jordan et al. : Pausing of mammalian cells by cold exposure, limits and opportunities. 2003. p. 67-70.
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D. L. Hacker; M. Cotten; F. Wurm : Molecular approaches to influence epigenetic effectors of transient and stable transgene expression in mammalian cells. 2003. p. 15-22.
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P. Girard; M. Derouazi; F. Wurm : Scalable, Serum-free, High Efficiency Transfection of CHO Cells using an Economical DNA Transfer Vehicle. 2003. p. 629-632.
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M. Derouazi; P. Girard; M. Jordan; C. Denoya; F. Wurm : Gene Transfer to animal cells in culture by microinjection: A novel tool to create recombinant cell lines?. 2003. p. 509-512.
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F. Grosjean / F. Wurm (Dir.) : Correlation between cell cycle and calcium phosphate transient transfection of CHO cells. Lausanne, EPFL, 2003. DOI : 10.5075/epfl-thesis-2829.
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R. Freitag; F. Garret-flaudy ; Affinity macroligands. EP1237940 ; US6258275 ; AU7540900 ; WO0125287 . 2002.
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M. Jordan; F. M. Wurm ; Cells, genetically modified with optical marker. US6509174 ; US2002058334 ; EP1088058 ; WO9966032 ; AU4054399 ; WO9966032 . 2002.
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J. Wright; F. Wurm ; Transfection using plasmid preparations. WO0226966 ; AU9014701 ; WO0226966 . 2002.
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P. Girard; M. Derouazi; G. Baumgartner; M. Bourgeois; M. Jordan et al. : 100-liter transient transfection; Cytotechnology. 2002. DOI : 10.1023/A:1021173124640.
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F. Grosjean; P. Batard; M. Jordan; F. Wurm : S-phase synchronized CHO cells show elevated transfection efficiency and expression using CaPi; Cytotechnology. 2002. DOI : 10.1023/A:1021197830091.
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F. Wurm; L. Baldi; P. Girard; J. Lindell; F. Grosjean et al. : Transient gene expression: A novel mammalian cell-based technology for recombinant protein production. A historical and technical perspective. 2002. p. 83-88.
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F. M. Wurm; J. Griffiths : Mammalian Cell Culture; The Encyclopedia of Physical Science and Technology; San Diego, Calif: Academic Press, 2002. p. 31-47.
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I. Bisson; J. Hilborn; F. Wurm; B. Meyrat; P. Frey : Human urothelial cells grown on collagen adsorbed to surface-modified polymers; Urology. 2002. DOI : 10.1016/S0090-4295(02)01642-4.
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H. M. Pick; P. Meissner; A. K. Preuss; P. Tromba; H. Vogel et al. : Balancing GFP reporter plasmid quantity in large-scale transient transfections for recombinant anti-human Rhesus-D IgG1 synthesis; Biotechnology and bioengineering. 2002. DOI : 10.1002/bit.10309.
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L. Hunt; P. Batard; M. Jordan; F. M. Wurm : Fluorescent proteins in animal cells for process development: optimization of sodium butyrate treatment as an example; Biotechnology and bioengineering. 2002. DOI : 10.1002/bit.10108.
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I. Bisson; M. Kosinski; S. Ruault; B. Gupta; J. Hilborn et al. : Acrylic acid grafting and collagen immobilization on poly(ethylene terephthalate) surfaces for adherence and growth of human bladder smooth muscle cells; Biomaterials. 2002. DOI : 10.1016/S0142-9612(02)00061-3.
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J. Wright / F. Wurm (Dir.) : Generation of plasmid DNA for the application of transient transfection in mammalian cells. Lausanne, EPFL, 2002. DOI : 10.5075/epfl-thesis-2486.
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I. Bisson / F. Wurm (Dir.) : Tissue engineering. Lausanne, EPFL, 2002. DOI : 10.5075/epfl-thesis-2477.
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P. Girard / F. Wurm (Dir.) : Rapid protein expression in mammalian cells. Lausanne, EPFL, 2002. DOI : 10.5075/epfl-thesis-2473.
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F. Wurm : Vers un nouveau médicament contre une grave maladie foetale; Flash EPFL. 2001.
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F. Wurm; A. Bernard : Transient gene expression from mammalian cells – a new chapter in animal cell technology?; Cytotechnology. 2001.
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F. Wurm : Spitzenforschung bleibt möglich; Biotech Forum. 2001.
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F. Grosjean; P. Batard; M. Jordan; F. Wurm : S-phase synchronized CHO cells show elevated transfection efficiency and expression using Ca/PO4. 2001. p. 238-240.
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L. Hunt; M. Jordan; F. Wurm : Sodium butyrate treatment: a study of critical parameters using CHO cell lines expressing the Green Fluorescent Protein. 2001. p. 318-323.
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M. De Jesus; L. Hunt; M. Bourgeois; L. Baldi; M. Jordan et al. : Na+ is responsible for intra-run variations of specific productivity of CHO cell lines producing human anti RhD-IgG in bioreactors. 2001. p. 193-196.
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M. De Jesus; M. Bourgeois; G. Baumgartner; P. Tromba; M. Jordan et al. : The Influence of pH on Cell Growth and Specific Productivity of Two CHO Cell Lines Producing Human Anti RhD IgG. 2001. p. 197-199.
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J. Wright; M. Jordan; F. Wurm : High effciency transfection of HEK 293 cells with impure plasmid DNA. 2001. p. 69-71.
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J. L. Wright; M. Jordan; F. Wurm : Extraction of plasmid DNA using reactor scale alkaline lysis and precipitation useful for transient transfection of animal cells; Cytotechnology. 2001. DOI : 10.1023/A:1013106032341.
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P. Girard; L. Porte; T. Berta; M. Jordan; F. Wurm : Calcium phosphate transfection optimization for serum-free suspension culture; Cytotechnology. 2001.