BiographySenior scientific level (R&D) with extensive experience in the management of multidisciplinary technological projects. Dr. Gerardo Turcatti, directs the academic technological platform, Biomolecular Screening Facility (BSF) at the EPFL he created in 2006. In the framework of the NCCR-Chemical Biology, he is project leader of the program ACCESS (An Academic Chemical Screening Platform for Switzerland). Previously he co-founded and acted as CTO of Manteia S.A., a Swiss-based company that developed high throughput DNA sequencing technologies currently owned by Illumina and used in the ‘Next Generation Sequencing’ instruments. Prior to this experience, Dr Turcatti had a long multidisciplinary career in R&D divisions of Biotechnology and Pharmaceutical companies with extensive expertise in several Chemical Biology-related disciplines such as Drug Screening, Chemical Biology, Bio-analytical Chemistry, DNA and Protein Chemistry. Dr Turcatti earned his Master in Chemical Engineering at the University of Geneva and his PhD in Chemistry and Biochemistry from the EPFL where he received the award for the best doctoral thesis of the year.
Extracted from Infoscience
High-throughput automated organoid culture via stem-cell aggregation in microcavity arraysNature Biomedical Engineering. 2020-06-08. DOI : 10.1038/s41551-020-0565-2.
Marker-Free Automatic Quantification of Drug-Treated Cardiomyocytes with Digital Holographic ImagingAcs Photonics. 2020-01-01. DOI : 10.1021/acsphotonics.9b01152.
Image-Based Marker-Free Screening of GABA(A) Agonists, Antagonists, and ModulatorsSlas Discovery. 2019-11-28. DOI : 10.1177/2472555219887142.
A Universal Assay for Aminopeptidase Activity and Its Application for Dipeptidyl Peptidase-4 Drug DiscoveryAnalytical Chemistry. 2019-01-01. DOI : 10.1021/acs.analchem.8b04672.
Quantification of stored red blood cell fluctuations by time-lapse holographic cell imagingBiomedical Optics Express. 2018-10-01. DOI : 10.1364/BOE.9.004714.
Targeting STING with covalent small-molecule inhibitorsNature. 2018. DOI : 10.1038/s41586-018-0287-8.
Red blood cells ageing markers: a multi-parametric analysisBlood Transfusion. 2017. DOI : 10.2450/2017.0318-16.
Screening out irrelevant cell-based models of diseaseNature Reviews Drug Discovery. 2016. DOI : 10.1038/nrd.2016.175.
Growth control switch by a DNA-damage-inducible toxin–antitoxin system in Caulobacter crescentusNature Microbiology. 2016. DOI : 10.1038/nmicrobiol.2016.8.
Development Of A Novel First-In-Class Oral Inhibitor Of The Notch Pathway2016. 21st Congress of the European-Hematology-Association, Copenhagen, DENMARK, JUN 09-12, 2016. p. 29-29.
Wnt directs the endosomal flux of LDL-derived cholesterol and lipid droplet homeostasisEmbo Reports. 2015. DOI : 10.15252/embr.201540081.
Identification of CDC-48 inhibitors in C. elegans2015. 40th Congress of the Federation-of-European-Biochemical-Societies (FEBS) - The Biochemical Basis of Life, Berlin, GERMANY, JUL 04-09, 2015. p. 389-389.
Digital Holographic Imaging for Label-Free Phenotypic Profiling, Cytotoxicity, and Chloride Channels Target ScreeningLabel-Free Biosensor Methods in Drug Discovery; New York: Springer, 2015. p. 307-325.
Detailed analysis and follow-up studies of a high-throughput screening for indoleamine 2,3-dioxygenase 1 (IDO1) inhibitorsEuropean Journal Of Medicinal Chemistry. 2014. DOI : 10.1016/j.ejmech.2014.06.078.
Developing the Biomolecular Screening Facility at the EPFL into the Chemical Biology Screening Platform for SwitzerlandCombinatorial Chemistry & High Throughput Screening. 2014.
Loss of Protein Tyr-phosphorylation During in vitro Storage of Human Erythrocytes: Impact on RBC Morphology2014. AABB Annual Meeting, Philadelphia, PA, OCT 25-28, 2014. p. 49A-50A.
Telomerase Inhibitors from Cyanobacteria: Isolation and Synthesis of Sulfoquinovosyl Diacylglycerols fromChemistry - A European Journal. 2013. DOI : 10.1002/chem.201203296.
Label-Free Cytotoxicity Screening Assay by Digital Holographic MicroscopyASSAY and Drug Development Technologies. 2013. DOI : 10.1089/adt.2012.476.
Chemical Biology Approaches to Membrane Homeostasis and FunctionChimia. 2011. DOI : 10.2533/chimia.2011.849.
Multiplexed detection of nucleic acids - testing and benchmarking of a novel platform developed by Biocartis2011
Leads for antitubercular compounds from kinase inhibitor library screensTuberculosis. 2010. DOI : 10.1016/j.tube.2010.09.001.
Preparation of nucleic acid templates for solid phase amplificationEP2918686 ; EP1957668 ; WO2007060456 ; US8168388 ; US2010041561 ; EP1957668 ; WO2007060456 ; GB0524069 . 2010.
Development of high content image-based cell viability assays2010
Cellular transfection of small interfering ribonucleic acids. Electroporation as an alternative to liposome-based delivery transfection methods2010
Preparation of templates for nucleic acid sequencingUS10710046 ; US2018353926 ; US9999866 ; US2017021325 ; US9498763 ; US2015343410 ; US9085802 ; US2014349891 ; US8715966 ; HK1154636 ; ES2398808 ; EP2298930 ; US2012208194 ; EP1910559 ; EP2298930 ; US2009118128 ; EP1910559 ; WO2007010251 ; WO2007010251 ; GB0514936 . 2009.
Accurate whole human genome sequencing using reversible terminator chemistryNature. 2008. DOI : 10.1038/nature07517.
Method of Nucleotide DetectionEP1828412 ; US9970055 ; US2016208324 ; US9303290 ; US9217178 ; US2014378323 ; EP1828412 ; US2008280773 ; EP1828412 ; WO2006064199 ; GB0514933 ; GB0427236 . 2008.
Low- to high-throughput analysis of telomerase modulators with TelospotNature Methods. 2007. DOI : 10.1038/nmeth1099.
Conversion of amine- to carboxyl groups on solid surfacesUS7704543 ; US2007042109 ; EP1692090 ; WO2005030695 . 2007.
Improved method of nucleotide detectionEP1828412 ; US9970055 ; US2016208324 ; US9303290 ; US9217178 ; US2014378323 ; EP1828412 ; US2008280773 ; EP1828412 ; WO2006064199 ; GB0514933 ; GB0427236 . 2007.
Aryldithioethyloxycarbonyl (Ardec): A new family of amine protecting groups removable under mild reducing conditions and their applications to peptide synthesisChemistry - A European Journal. 2006. DOI : 10.1002/chem.200501538.
A microfluidic platform using molecular beacon-based temperature calibration for thermal dehybridization of surface-bound DNAAnalytical Chemistry. 2004. DOI : 10.1021/ac034377+.
Microfluidics devices for heterogeneous assaysUniversité de Neuchâtel, 2003.
Nucleotide detection by fluorescence resonance energy transfer and methods thereofBR0303224 ; NO20034592 ; NO20034592 ; AU2003226829 ; WO03068986 . 2003.
Development of a Microfluidic Device for Genetic Analysis Based on Hybridization of Surface-bound DNA2002.
Synthesis and characterization of fluorescent and photoactivatable MIP-1alpha ligands and interactions with chemokine receptors CCR1 and CCR5Journal of Medicinal Chemistry. 2001. DOI : 10.1021/jm000982i.
Methods of nucleic acid amplification and sequencingUS10370652 ; US2016319274 ; US9297006 ; US2014228254 ; US8652810 ; EP1634963 ; EP1117827 ; US2008160580 ; US7115400 ; DE69928683 ; EP1634963 ; DE69928683 ; AT311474 ; EP1117827 ; AU771073 ; EA004271 ; HU0105052 ; NZ510599 ; HK1042525 ; JP2002525125 ; AR021833 ; EE200100195 ; HU0105052 ; PL347125 ; IL142178 ; BG105363 ; CN1325458 ; SK4292001 ; EA200100402 ; ZA200102420 ; CZ20011183 ; KR20010085860 ; EP1117827 ; TR200100911 ; BR9914159 ; NO20011303 ; NO20011303 ; AU6108899 ; CA2344575 ; WO0018957 . 2000.
Fluorescent pseudo-peptide linear vasopressin antagonists: Design, synthesis, and applicationsJournal of Medicinal Chemistry. 1999. DOI : 10.1021/jm9804782.
Biophysical approaches to G protein-coupled receptors: Structure, function and dynamicsJournal of Computer-Aided Molecular Design. 1999. DOI : 10.1023/A:1008052002695.
Mapping the binding sites of peptide and non-peptide molecules to G protein-coupled receptors by fluorescenceLetters in Peptide Science. 1998.
Probing ligand-receptor recognition in G protein-coupled receptors through biosynthetic incorporation of fluorescent amino acids at specific sites and measurement of distances by fluorescence energy transfer1998. 2nd Conference on Fluorescence Microscopy and Fluorescent Probes, Prague, Apr. 9-12, 1997. p. 87-92.
Characterization of non-peptide antagonist and peptide agonist binding sites of the NK1 receptor with fluorescent ligandsJournal of Biological Chemistry. 1997. DOI : 10.1074/jbc.272.34.21167.
Fluorescent labeling of NK2 receptor at specific sites in vivo and fluorescence energy transfer analysis of NK2 ligand-receptor complexesReceptors and Channels. 1997.
Probing the structure and function of the tachykinin neurokinin-2 receptor through biosynthetic incorporation of fluorescent amino acids at specific sitesJournal of Biological Chemistry. 1996.
Probing the Binding Domain of the NK2 Receptor with Fluorescent Ligands: Evidence That Heptapeptide Agonists and Antagonists Bind DifferentlyBiochemistry. 1995. DOI : 10.1021/bi00012a015.
Purification, cDNA cloning and heterologous expression of human phosphomannose isomeraseEuropean Journal of Biochemistry. 1994. DOI : 10.1111/j.1432-1033.1994.tb19954.x.
Synthesis and characterization of selective fluorescent ligands for the neurokinin NK2 receptorJournal of Medicinal Chemistry. 1994. DOI : 10.1021/jm00039a012.
Biochemical characterization and solubilization of human NK2 receptor expressed in Chinese hamster ovary cellsJournal of Receptor Research. 1993.
Role of cysteine62 in DNA recognition by the p50 subunit of NF-kappa BNucleic Acids Research. 1993. DOI : 10.1093/nar/21.8.1727.
Partial characterization of natural and recombinant human soluble CD23Biochemical Journal. 1992.
Purification and characterization of biologically active human recombinant 37 kDa soluble CD23 (sFc epsilon RII) expressed in insect cellsJournal of Immunological Methods. 1992. DOI : 10.1016/0022-1759(92)90253-P.
Human interleukin-5 expressed in Escherichia coli: Assignment of the disulfide bridges of the purified unglycosylated proteinFEBS Letters. 1991. DOI : 10.1016/0014-5793(91)80553-F.
Purification and partial characterization of rat factor DBiochemical Journal. 1991.
Tumor necrosis factor inhibitor: purification, NH2-terminal amino acid sequence and evidence for anti-inflammatory and immunomodulatory activitiesEuropean Journal of Immunology. 1990.
Structure and partial amino acid sequence of calf thymus DNA topoisomerase II: Comparison with other type II enzymesBiochemical and Biophysical Research Communications. 1990. DOI : 10.1016/0006-291X(90)92156-T.
Purification and characterization of a methionine-specific aminopeptidase from Salmonella typhimuriumEuropean Journal of Biochemistry. 1989. DOI : 10.1111/j.1432-1033.1989.tb14610.x.
Characterization of recombinant-derived granulocyte-colony stimulating factor (G-CSF)Biochemical Journal. 1988.
Insulin proteinase liberates from glucagon a fragment known to have enhanced activity against Ca2+ + Mg2+-dependent ATPaseBiochemical Journal. 1988.
Teaching & PhD
Life Sciences Engineering