Graham Knott
Mission
Graham is a senior scientist and head of the BioEM Facility within the Centre of Electron Microscopy at the EPFL. This platform comprises a wide variety of electron imaging technologies and preparation methods that enable EPFL researchers to image all types of biological samples with electrons.
Biography
Graham Knott received his degree in physiology from the University of Southampton, UK, in 1990, and his PhD in neuroscience from the University of Tasmania, Australia, in 1995. He moved to the University of Lausanne in Switzerland in 1999 where he researched the plasticity of neuronal connectivity in the adult brain, developing correlative light and electron microscopy methods for the analysis of in vivo imaged neurons. In 2006 Graham joined the Ecole Polytechnique Fédérale de Lausanne, establishing the Bio Electron Microscopy Facility and has continued his research interests in brain plasticity and 3D electron microscopy.
Enseignement & Phd
Enseignement
- Life Sciences Engineering,
Programmes doctoraux
- Doctoral Program in Neuroscience
Doctorants
Cours
Electron Microscopy for Life Science
This is a two-day course. It consists of seven lectures, and 2 afternoon practical/demonstration sessions that covers the principles of preparing biological samples from electron microscopy and a basic introduction to using scanning and transmission elect... 
Recherche
Pubications during last 5 years
PEER REVIEWED RESEARCH
Pino, E., Amamoto, R., Zheng, L., Cacquevel, M., Sarria, J.C., Knott, G.W., and Schneider, B.L. (2013). FOXO3 determines the accumulation of ?-synuclein and controls the fate of dopaminergic neurons in the substantia nigra. Hum Mol Genet
Allegra Mascaro, A.L., Cesare, P., Sacconi, L., Grasselli, G., Mandolesi, G., Maco, B., Knott, G.W., Huang, L., De Paola, V., Strata, P., and Pavone, F.S. (2013). In vivo single branch axotomy induces GAP-43-dependent sprouting and synaptic remodeling in cerebellar cortex. Proc Natl Acad Sci U S A 110, 10824-10829.
Canty, A.J., Huang, L., Jackson, J.S., Little, G.E., Knott, G., Maco, B., and De Paola, V. (2013). In-vivo single neuron axotomy triggers axon regeneration to restore synaptic density in specific cortical circuits. Nat Commun 4, 2038.
Grillo, F.W., Song, S., Teles-Grilo Ruivo, L.M., Huang, L., Gao, G., Knott, G.W., Maco, B., Ferretti, V., Thompson, D., Little, G.E., and De Paola, V. (2013). Increased axonal bouton dynamics in the aging mouse cortex. Proc Natl Acad Sci U S A 110, E1514-E1523.
Houtkooper, R.H., Mouchiroud, L., Ryu, D., Moullan, N., Katsyuba, E., Knott, G., Williams, R.W., and Auwerx, J. (2013). Mitonuclear protein imbalance as a conserved longevity mechanism. Nature 497, 451-457.
Knott, G.W. (2013). Imaging green fluorescent protein-labeled neurons using light and electron microscopy. Cold Spring Harb Protoc 2013, 542-550.
Maco, B., Holtmaat, A., Cantoni, M., Kreshuk, A., Straehle, C.N., Hamprecht, F.A., and Knott, G.W. (2013). Correlative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical neurons. PLoS One 8, e57405.
Mostany, R., Anstey, J.E., Crump, K.L., Maco, B., Knott, G., and Portera-Cailliau, C. (2013). Altered synaptic dynamics during normal brain aging. J Neurosci 33, 4094-4104.
Xiao, L., Michalski, N., Kronander, E., Gjoni, E., Genoud, C., Knott, G., and Schneggenburger, R. (2013). BMP signaling specifies the development of a large and fast CNS synapse. Nat Neurosci 16, 856-864.
Becker, C., Ali, K., Knott, G., and Fua, P. (2012). Learning context cues for synapse segmentation in EM volumes. Med Image Comput Comput Assist Interv 15, 585-592.
Cheetham, C.E., Barnes, S.J., Albieri, G., Knott, G.W., and Finnerty, G.T. (2012). Pansynaptic Enlargement at Adult Cortical Connections Strengthened by Experience. Cereb Cortex
Gaugler, M.N., Genc, O., Bobela, W., Mohanna, S., Ardah, M.T., El-Agnaf, O.M., Cantoni, M., Bensadoun, J.C., Schneggenburger, R., Knott, G.W., Aebischer, P., and Schneider, B.L. (2012). Nigrostriatal overabundance of ?-synuclein leads to decreased vesicle density and deficits in dopamine release that correlate with reduced motor activity. Acta Neuropathol
Mikeladze-Dvali, T., von Tobel, L., Strnad, P., Knott, G., Leonhardt, H., Schermelleh, L., and Gönczy, P. (2012). Analysis of centriole elimination during C. elegans oogenesis. Development 139, 1670-1679.
Sznitman, R., Lucchi, A., Pjescic-Emedji, N., Knott, G., and Fua, P. (2012). Efficient scanning for EM based target localization. Med Image Comput Comput Assist Interv 15, 337-344.
Wu, X., Fu, Y., Knott, G., Lu, J., Di Cristo, G., and Huang, Z.J. (2012). GABA signaling promotes synapse elimination and axon pruning in developing cortical inhibitory interneurons. J Neurosci 32, 331-343.
Kreshuk, A., Straehle, C.N., Sommer, C., Koethe, U., Cantoni, M., Knott, G., and Hamprecht, F.A. (2011). Automated detection and segmentation of synaptic contacts in nearly isotropic serial electron microscopy images. PLoS One 6, e24899.
Knott, G., Rosset, S., and Cantoni, M. (2011). Focussed ion beam milling and scanning electron microscopy of brain tissue. J Vis Exp
Landers, M.S., Knott, G.W., Lipp, H.P., Poletaeva, I., and Welker, E. (2011). Synapse formation in adult barrel cortex following naturalistic environmental enrichment. Neuroscience 199, 143-152.
Lucchi, A., Smith, K., Achanta, R., Knott, G., and Fua, P. (2011). Supervoxel-Based Segmentation of Mitochondria in EM Image Stacks With Learned Shape Features. IEEE Trans Med Imaging 31, 474-486.
Straehle, C.N., Köthe, U., Knott, G., and Hamprecht, F.A. (2011). Carving: scalable interactive segmentation of neural volume electron microscopy images. Med Image Comput Comput Assist Interv 14, 653-660.
Holtmaat, A., Bonhoeffer, T., Chow, D.K., Chuckowree, J., De Paola, V., Hofer, S.B., Hubener, M., Keck, T., Knott, G., Lee, W.C., Mostany, R., Mrsic-Flogel, T.D., Nedivi, E., Portera-Cailliau, C., Svoboda, K., Trachtenberg, J.T., and Wilbrecht, L. (2009). Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window. Nat Protoc 4, 1128-1144.
Knott, G.W., Holtmaat, A., Trachtenberg, J.T., Svoboda, K., and Welker, E. (2009). A protocol for preparing GFP-labeled neurons previously imaged in vivo and in slice preparations for light and electron microscopic analysis. Nat Protoc 4, 1145-1156.
Zito, K., Scheuss, V., Knott, G., Hill, T., and Svoboda, K. (2009). Rapid functional maturation of nascent dendritic spines. Neuron 61, 247-258.
REVIEW
Knott, G., and Genoud, C. (2013). Is EM dead? J Cell Sci 126, 4545-4552