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Julien Maillard

julien.maillard@epfl.ch

Nationalité: Swiss

EPFL › ENAC › IIE › LBE

Site web: https://lbe.epfl.ch/

Expertise

- Anaerobic microbiology
- Bioremediation
- Molecular biology
- Biochemistry

Travail en cours

Current projects: - Role of the gene products from the pceABC gene cluster in Dehalobacter restrictus - Characterization of RdhK proteins involved in the transcriptional regulation of organohalide respiration in Dehalobacter restrictus (with Mathilde Willemin, PhD student at LBE). - Tetrachloroethene dechlorinating consortia - competition with other anaerobic respiration pathways (with Alexandra Murray, DTU, Denmark) - Microbiology of aerobic granular sludge for wastewater treatment (with Aline Alder and Arnaud Gelb, PhD students of LBE). - Tetrasphaera as possible key player in phosphate removal in wastewater treatment (initiated with Marta Cerruti, Master student at LBE). External collaborations: - Phosphate metabolism of Shewanella loihica in iron reduction studies for the conservation of artefact objects (with Wafa Kooli, Edith Joseph and Pilar Junier, University of Neuchâtel). On-going. - Desulfitobacterium as biological tool for the restoration of iron artefact objects (with Lucrezia Comensoli, Edith Joseph and Pilar Junier, University of Neuchâtel). Completed in 2017. - arsM gene diversity in rice paddy soil for arsenic methylation (with Matthew Reid, Leia Falquet and Rizlan Bernier-Latmani, Environmental Microbiology Laboratory, EPFL). Completed in 2017.

Mission

Main project: Physiology and biochemistry of the tetrachloroethene anaerobic respiration pathway (organohalide respiration). A subset of obligate anaerobic bacteria are able to use chlorinated compounds such as tetrachloroethene (PCE) as terminal electron acceptors. This process, coined 'organohalide respiration', involves the establishment of a dedicated respiratory chain, in which the PCE reductive dehalogenase (PceA) plays a central and catalytic role. On-going work focuses on the study of the proteins involved in PCE respiration and on molecular aspects of this metabolism. In addition to pure cultures, bacterial consortia are investigated for the coexistence of PCE dechlorinating populations and their succession in the stepwise dechlorination of PCE.
2011 - present: Research associate at the Laboratory for Environmental Biotechnology (LBE, IIE-ENAC, EPFL) 2007 - 2010: Postdoctoral Fellow at the Laboratory for Environmental Biotechnology (LBE, IIE-ENAC, EPFL) 2005 - 2006: Postdoctoral Fellow at the University of East Anglia (UEA, Norwich, UK) 2000 - 2004: PhD thesis at the Laboratory for Environmental Biotechnology (LBE) 1995 - 2000: B.sc & M.sc at the Swiss Federal Institute for Technology, Zurich (ETHZ)

CONFERENCES & TALKS

2017 - Annual Assembly of the Swiss Society of Microbiology, Basel, Switzerland (2 Talks and 3 Posters). - DehaloCon, Conference on dehalogenation, Leipzig, Germany (1 Poster and 1 Talk). 2016 - International Symposium on Biopolymers, Madrid, Spain (1 Poster). - Annual Assembly of the Swiss Society of Microbiology, Bern, Switzerland (1 Talk and 2 Posters). - Annual Conference of the Association for General and Applied Microbiology (VAAM), Jena, Germany (1 Poster). 2015 - FEMS Microbiology Congress, Geneva, Switzerland (1 Poster). - Swiss Microbial Ecology meeting, Ascona, Switzerland (2 Posters and 1 Talk). - Bacterial electron transport processes and their regulation, Vimeiro, Portugal (2 Posters). 2014 - Annual Assembly of the Swiss Society of Microbiology, Fribourg, Switzerland (3 Posters). - DehaloCon, Conference on dehalogenation, Jena, Germany (1 Poster and 3 Talks). - Invited talk given at the University of Strasbourg. 2013 - FEMS Microbiology Congress, Leipzig, Germany (2 Posters). - DFG Research Unit FOR 1530 PhD Conference, UFZ Leipzig, Germany (Invited talk). - Annual Assembly of the Swiss Society of Microbiology, Interlaken, Switzerland (2 Posters). 2012 - 6th Swiss Molecular Microbiology Meeting, Villars-sur-Ollon, Switzerland (2 Posters). - Annual Assembly of the Swiss Society of Microbiology, St-Gallen, Switzerland (3 Posters). 2011 - International Scientific Seminar: Understanding and application of organohalide respiration: from genomes to structures, Kavli Centre, UK (Invited talk). - FEMS 2011, Geneva, Switzerland (Poster) - European Workshop on Bacterial Respiratory Chains, Höör, Sweden (Talk). 2010 - Gordon Research Conference on Molecular Basis Of Microbial One-Carbon Metabolism, Bates College, Lewiston ME, USA (Poster). - Annual Assembly of the Swiss Society of Microbiology, Zurich, Switzerland (Poster). - Minisymposium on Functional Genomics of Organohalide Respiring Bacteria, University of Wageningen, The Netherlands (Invited talk). 2009 - EMBO-FEMS Workshop on Microbial Sulfur Metabolism, Tomar, Portugal (Poster). - Annual Assembly of the Swiss Society of Microbiology, Lausanne, Switzerland (Poster). - Gordon Research Conference on Vitamin B12 and Corphins, Magdalen College, Oxford, UK (Poster). 2008 - Annual Assembly of the Swiss Society of Microbiology, Interlaken, Switzerland (Poster talk).

STUDENT GUIDANCE

PhD students: - Lorenzo Cimmino, EPFL-LBE. PhD thesis. Co-direction. On-going. - Mathilde Willemin, EPFL-LBE. PhD thesis. Co-direction. On-going. - Alexandra Murray, Denmark Technical University (DTU). PhD thesis. On-going. - Géraldine Buttet, EPFL-LBE. PhD thesis. Co-direction. 2017. - Aamani Rupakula, EPFL-LBE. PhD thesis. Co-direction. 2015. - Laure Prat, EPFL-LBE. PhD thesis. Co-direction. 2009. Master students: - Marta Cerruti, Université de Turin, Italie. 10-month Master. Main supervision. 2017. - Corantin Grob, Université de Strasbourg, France. 5-month Master-2 thesis. Main supervision. 2015. - Arnaud Gelb, Université de Strasbourg, France. 5-month Master-2 thesis. Main supervision. 2014. - Laura Frutieaux, Université de Pau et des Pays de l'Adour, France. 2-month Master-1 thesis. Main supervision. 2013. - Chloé Bennati-Granier, University of Paris-Sud, France. 6-month Master thesis. Co-direction. 2012 - Géraldine Buttet, University of Neuchâtel, Switzerland. 12-month Master thesis. Main supervision. 2011-2 - Aamani Rupakula, Velore University, India. 5-month Master thesis. Main supervision. 2010. - Ariane Kull, University of Applied Sciences, HES-SO, Sion, Switzerland. 5-month Master thesis. Main supervision. 2009. Bachelor students: - Isabel Jankowski, University of British Columbia, Vancouver, Canada. 3-month internship. 2017. - Selima Zahar, EPFL, SV. 14-day semester project. 2017. - Antsa Rabenifara Estoppey, HES-SO, Sion, Switzerland. 2.5-month Bachelor project. Co-supervision with Magalie Bassan. 2015. - Kiyan Shabestary, EPFL-SV, Lausanne, Switzerland. Semester project. 2013. Internship students: - Ziad Julier, EPFL. 3-month civil service. 2015-2016. - Clément Descarpentries, Université de Strasbourg, France. 2-month internship. 2015. - Cindy Kunze, University of Jena, Germany. 2-month internship. 2013. - Alexandre Chabrelie, PolyTech Grenoble, France. 3-month internship. Co-supervision with David Weissbrodt. 2011. - Christelle Petit, PolyTech Clermont, France. 3-month internship. 2010. - Marion Blondeau, PolyTech Clermont, France. 3-month internship. 2008.

Publications représentatives

(40) The membrane-bound C subunit of reductive dehalogenases: topology analysis and reconstitution of the FMN-binding domain of PceC

Buttet GF, Willemin MS, Hamelin R, Rupakula, Maillard J
Published in Frontiers in Microbiology, in press in

(39) Coexistence of two distinct Sulfurospirillum populations respiring tetrachloroethene - genomic and kinetic considerations

Buttet GF, Murray AM, Goris T, Burion M, Jin B, Rolle M, Holliger C, Maillard J
Published in FEMS Microbiol Ecol. 2018 Mar 6. doi: 10.1093/femsec/fiy018 in

(38) Bacterial iron reduction and biogenic mineral formation for the stabilisation of corroded iron objects

Kooli WM, Comensoli L, Maillard J, Albini M, Gelb A, Junier P, Joseph E
Published in Sci Rep. 2018 Jan 15;8(1):764. doi: 10.1038/s41598-017-19020-3 in

(37) Comparative genomics of the genus Desulfitobacterium

Kruse T, Goris T, Maillard J, Woyke T, Lechner U, de Vos W, Smidt H
Published in FEMS Microbiol Ecol. 2017 Dec 1;93(12). doi: 10.1093/femsec/fix135 in

(36) Arsenic Methylation Dynamics in a Rice Paddy Soil Anaerobic Enrichment Culture

Reid MC, Maillard J, Bagnoud A, Falquet L, Le Vo P, Bernier-Latmani R
Published in Environ Sci Technol. 2017 Sep 19;51(18):10546-10554. doi: 10.1021/acs.est.7b02970 in

(35) Use of Bacteria To Stabilize Archaeological Iron

Comensoli L, Maillard J, Albini M, Sandoz F, Junier P, Joseph E
Published in Appl Environ Microbiol. 2018 Apr 17;83(9): e03478-16. doi: 10.1128/AEM.03478-16 in

(34) The Genus Dehalobacter

Maillard J, Holliger C
Published in Book chapter in Organohalide-Respiring Bacteria (Adrian & Löffler. Editors), Springer-Verlag, 2016. in

(33) Functional genomics of corrinoid starvation in the organohalide-respiring bacterium Dehalobacter restrictus strain PER-K23

Rupakula A, Lu Y, Kruse T, Boeren S, Holliger C, Smidt H, Maillard J
Published in Front. Microbiol. 2015 5:751 in

(32) Nitrogen removal over nitrite by aeration control in aerobic granular sludge sequencing batch reactors

Lochmatter S, Maillard J, Holliger C.
Published in Int. J. Environ. Res. Public Health 2014, 11(7), 6955-6978; doi:10.3390/ijerph110706955 in

(31) Multiple dual C-Cl isotope patterns associated with reductive dechlorination of tetrachloroethene

Badin A, Buttet G, Maillard J, Holliger C, Hunkeler D.
Published in Environ Sci Technol. 2014 Jul 7. [Epub ahead of print]. in

(30) Multilevel correlations in the biological phosphorus removal process: From bacterial enrichment to conductivity-based metabolic batch tests and polyphosphatase assays

Weissbrodt DG, Maillard J, Brovelli A, Chabrelie A, May J, Holliger C.
Published in Biotechnol Bioeng. 2014. Ahead of pub. DOI: 10.1002/bit.25320 in

(29) Use of silicate minerals for long-term pH control during reductive dechlorination of high tetrachloroethene concentrations in continuous flow-through columns

Lacroix E, Brovelli A, Maillard J, Rohrbach-Brandt E, Barry DA, Holliger C
Published in Sci Total Environ. 2014 Jun 1;482-483:23-35 in

(28) Fe(III) reduction during pyruvate fermentation by Desulfotomaculum reducens strain MI-1

Fe(III) reduction during pyruvate fermentation by Desulfotomaculum reducens strain MI-1. Dalla Vecchia E, Suvorova EI, Maillard J, Bernier-Latmani R.
Published in Geobiology. 2014 Jan;12(1):48-61. doi: 10.1111/gbi.12067 in

(27) Bacterial versus fungal laccase: potential for micropollutant degradation

Margot J, Bennati-Granier C, Maillard J, Blánquez P, Barry DA, Holliger C.
Published in AMB Express 2013, Oct; 3(1):63-76. in

(26) Diversity of cobalamin riboswitches in the corrinoid-producing organohalide respirer Desulfitobacterium hafniense

Choudhary PK, Duret A, Rohrbach-Brandt E, Holliger C, Sigel RKO, Maillard J.
Published in J Bacteriol. 2013 Sep [Epub ahead of pub] DOI: 10.1128/JB.00730-13 in

(25) Functional genotyping of Sulfurospirillum spp. in mixed cultures allowed the identification of a new tetrachloroethene reductive dehalogenase

Buttet GF, Holliger C, Maillard J
Published in Appl Environ Microbiol. 2013 Aug 30;79(22):6941-7. in

(24) Complete genome sequence of Dehalobacter restrictus PER-K23T

Kruse T, Maillard J, Goodwin L,Woyke T, Teshima H, Bruce D, Detter C, Tapia R, Han C, Huntemann M, Wei CL, Han J, Chen A, Kyrpides N, Szeto E, Markowitz V, Ivanova N, Pagani I, Pati A, Pitluck S, Nolan M, Holliger C, Smidt H
Published in Stand. Genomic Sci. 2013 8:3 in

(23) Influence of treatment conditions on the oxidation of micropollutants by Trametes versicolor laccase

Margot J, Maillard J, Rossi L, Barry DA, Holliger C.
Published in N Biotechnol. 2013 Jul 3;30(6):803-13. DOI: 10.1016/j.nbt.2013.06.004 in

(22) The restricted metabolism of the obligate organohalide respiring bacterium Dehalobacter restrictus: lessons from tiered functional genomics

Rupakula A, Kruse T, Boeren S, Holliger C, Smidt H, Maillard J.
Published in Philos Trans R Soc Lond B Biol Sci. 2013 Mar 11;368(1616):20120325 in

(21) PyroTRF-ID: a novel bioinformatics methodology for the affiliation of terminal-restriction fragments using 16S rRNA gene pyrosequencing data

Weissbrodt DG, Shani N, Sinclair L, Lefebvre G, Rossi P, Maillard J, Rougemont J, Holliger C
Published in BMC Microbiol. 2012 Dec 27;12(1):306 in

(20) Segmented field OFFGEL® electrophoresis

Tobolkina E, Cortés-Salazar F, Momotenko D, Maillard J, Girault HH
Published in Electrophoresis. 2012 Nov;33(22):3331-8 in

(19) Bacterial Selection during the Formation of Early-Stage Aerobic Granules in Wastewater Treatment Systems Operated Under Wash-Out Dynamics

Weissbrodt DG, Lochmatter S, Ebrahimi S, Rossi P, Maillard J, Holliger C
Published in Front Microbiol. 2012;3:332 in

(18) The opportunistic physiology of Desulfitobacterium hafniense strain TCE1 towards organohalide respiration with tetrachloroethene

Duret A, Holliger C, Maillard J.
Published in Appl Environ Microbiol. 2012 Sep;78(17):6121-7 in

(17) An unusual tandem-domain rhodanese harbouring two active sites identified in Desulfitobacterium hafniense

Prat L, Maillard J, Rohrbach-Brandt E, Holliger C
Published in FEBS J. 2012 Aug;279(15):2754-67 in

(16) Overlapping transport and chaperone-binding functions within a bacterial twin-arginine signal peptide

Grahl S, Maillard J, Spronk CAEM, Vuister GW, Sargent F.
Published in Mol Microbiol. 2012; 83(6):1254-1267 in

(15) Redundancy and specificity of multiple Trigger Factor chaperones in Desulfitobacteria

Maillard J, Genevaux P, Holliger C.
Published in Microbiology (SGM). 2011 Aug;157(8):2410-21 in

(14) Physiological adaptation of Desulfitobacterium hafniense strain TCE1 to tetrachloroethene respiration

Prat L, Maillard J, Grimaud R, Holliger C.
Published in Appl Environ Microbiol. 2011. 77(11):3853-9 in

(13) Reductive dechlorination of tetrachloroethene by a stepwise catalysis of different organohalide respiring bacteria and reductive dehalogenases

Maillard J, Charnay M-P, Regeard C, Rohrbach-Brandt E, Rouzeau-Szynalski K, Rossi P, Holliger C
Published in Biodegradation 2011; 22:949-60 in

(12) Frequent concomitant presence of Desulfitobacterium spp. and “Dehalococcoides” spp. in chloroethene-dechlorinating microbial communities

Rouzeau-Szynalski K, Maillard J, Holliger C
Published in Appl Microbiol Biotechnol. 2011. 90:361-68 in

(11) Performance and microbial community composition dynamics of aerobic granular sludge from sequencing batch bubble column reactors operated at 20, 30, and 35°C

Ebrahimi S, Gabus S, Rohrbach-Brandt E, Hosseini M, Rossi P, Maillard J, Holliger C
Published in Appl Microbiol Biotechnol. 2010 Jul;87(4):1555-68 in

(10) Intrinsic GTPase activity of a bacterial twin-arginine translocation proofreading chaperone induced by domain swapping

Guymer D, Maillard J, Agacan MF, Brearley CA, Sargent F
Published in FEBS J. 2010 277(2):511-25 in

(9) A genetic analysis of in vivo selenate reduction by Salmonella enterica serovar Typhimurium LT2 and Escherichia coli K12

Guymer D, Maillard J, Sargent F
Published in Arch Microbiol 2009 191(6):519-28 in

(8) Features of a twin-arginine signal peptide required for recognition by a Tat proofreading chaperone

Buchanan G, Maillard J, Nabuurs SB, Richardson DJ, Palmer T, Sargent S
Published in FEBS Letters 2008 582:3979–3984 in

(7) Structural diversity in twin-arginine signal peptide-binding proteins

Maillard J, Spronk CA, Buchanan G, Lyall V, Richardson DJ, Palmer T, Vuister GW, Sargent F.
Published in Proc Natl Acad Sci U S A. 2007 Oct 2;104(40):15641-6 in

(6) Indications for acquisition of reductive dehalogenase genes through horizontal gene transfer by Dehalococcoides ethenogenes strain 195

Regeard C, Maillard J, Dufraigne C, Deschavanne P, Holliger C.
Published in Appl Environ Microbiol. 2005 Jun;71(6):2955-61 in

(5) Development of a real-time PCR method for quantification of the three genera Dehalobacter, Dehalococcoides, and Desulfitobacterium in microbial communities

Smits TH, Devenoges C, Szynalski K, Maillard J, Holliger C.
Published in J Microbiol Methods. 2004 Jun;57(3):369-78 in

(4) Isolation and characterization of Tn-Dha1, a transposon containing the tetrachloroethene reductive dehalogenase of Desulfitobacterium hafniense strain TCE1

Maillard J, Regeard C, Holliger C.
Published in Environ Microbiol. 2005 Jan;7(1):107-17 in

(3) Development of degenerate and specific PCR primers for the detection and isolation of known and putative chloroethene reductive dehalogenase genes

Regeard C, Maillard J, Holliger C.
Published in J Microbiol Methods. 2004 Jan;56(1):107-18 in

(2) Characterization of the corrinoid iron-sulfur protein tetrachloroethene reductive dehalogenase of Dehalobacter restrictus

Maillard J, Schumacher W, Vazquez F, Regeard C, Hagen WR, Holliger C.
Published in Appl Environ Microbiol. 2003 Aug;69(8):4628-38 in

(1) Dichloromethane mediated in vivo selection and functional characterization of rat glutathione S-transferase theta 1-1 variants

Gisi D, Maillard J, Flanagan JU, Rossjohn J, Chelvanayagam G, Board PG, Parker MW, Leisinger T, Vuilleumier S.
Published in Eur J Biochem. 2001 Jul;268(14):4001-10 in