Lucia Baldi Unser
Nationality: Italian and Swiss
AVP-DLE-EDOC
CE 1 625 (Centre Est)
Station 1
1015 Lausanne
+41 21 693 55 62
Office: CE 1 625
EPFL › VPA › VPA-AVP-DLE › AVP-DLE-EDOC › EDOC-GE
Website: https://www.epfl.ch/education/phd/
Expertise
Management of academic organizations
Communication & events management
Gender equality promotion in academia
Past research activities:
Mammalian cells biotechnology ; Biopharmaceuticals ; Bioprocess engineering ; Non-viral gene delivery ; Stable cell lines development ; Gene expression and regulation ; Molecular immunology.
Communication & events management
Gender equality promotion in academia
Past research activities:
Mammalian cells biotechnology ; Biopharmaceuticals ; Bioprocess engineering ; Non-viral gene delivery ; Stable cell lines development ; Gene expression and regulation ; Molecular immunology.
01/2021 - 04/2022 Deputy to the Associate VP for Research / Deputy to the Associate VP for Centers & Platforms, EPFL
06/2017 - 12/2020 Deputy to the Dean, School of Basic Sciences, Ecole Polytechnique Fédérale de Lausanne ;
01/2015 - 11/2017 Lecturer/Communication and outreach manager at the School of Life Sciences, EPFL
04/1998 –- 03/2015: Research and Teaching Associate/Lecturer, Laboratory of Cellular Biotechnology, School of Life Sciences; 01/1994 -– 07/1997: Visiting Fellow, Laboratory of Immunoregulation, NIAID, NIH, Bethesda, Maryland, USA;
1991 - 1994: Research Fellow, Laboratory of Pediatric Oncology, G. Gaslini Scientific Institute, Genova, Italy;
1987 - 1990: Research Fellow, Clinical Immunology and Immunogenetics Dept., Nat. Inst. for Cancer Research, Genova, Italy.
06/2017 - 12/2020 Deputy to the Dean, School of Basic Sciences, Ecole Polytechnique Fédérale de Lausanne ;
01/2015 - 11/2017 Lecturer/Communication and outreach manager at the School of Life Sciences, EPFL
04/1998 –- 03/2015: Research and Teaching Associate/Lecturer, Laboratory of Cellular Biotechnology, School of Life Sciences; 01/1994 -– 07/1997: Visiting Fellow, Laboratory of Immunoregulation, NIAID, NIH, Bethesda, Maryland, USA;
1991 - 1994: Research Fellow, Laboratory of Pediatric Oncology, G. Gaslini Scientific Institute, Genova, Italy;
1987 - 1990: Research Fellow, Clinical Immunology and Immunogenetics Dept., Nat. Inst. for Cancer Research, Genova, Italy.
Education
PhD (Board Certification)
| Clinical Immunology and Allergy1994 – 1994 University of Genova, Italy, and NIAID, NIH, Bethesda MD, USA
MS, Biology
| Microbiology1986 – 1986 University of Genova, Italy
Publications of the last years at EPFL
2018
Improved process conditions for increasing expression of MHC class II protein from a stable Drosophila S2 cell line
BIOTECHNOLOGY LETTERS. 2018. DOI : 10.1007/s10529-017-2440-0.2016
A comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation
Biotechnology Progress. 2016. DOI : 10.1002/btpr.2328.User-Friendly Image-Based Segmentation and Analysis of Chromosomes
2016. p. 395-398. DOI : 10.1109/ISBI.2016.7493291.Comparison of Three Transposons for the Generation of Highly Productive Recombinant CHO Cell Pools and Cell Lines
Biotechnology And Bioengineering. 2016. DOI : 10.1002/bit.25888.Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension
Analytical Biochemistry. 2016. DOI : 10.1016/j.ab.2016.04.009.2015
Enhanced Plasmid DNA Utilization in Transiently Transfected CHO-DG44 Cells in the Presence of Polar Solvents
Biotechnology Progress. 2015. DOI : 10.1002/btpr.2152.A simple plasmid-based transient gene expression method using High Five cells
Journal Of Biotechnology. 2015. DOI : 10.1016/j.jbiotec.2015.10.007.Transient Production of Recombinant Adeno-Associated Virus (AAV) Vectors for Gene Therapy Applications Using Suspension-Adapted HEK 293 Cells in Orbital Shaken Bioreactors
2015. 18th Annual Meeting of the American-Society-of-Gene-and-Cell-Therapy (ASGCT)'. p. S184-S185.Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools
Journal of Biotechnology. 2015. DOI : 10.1016/j.jbiotec.2015.03.001.Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells
Biotechnology Progress. 2015. DOI : 10.1002/btpr.2064.2014
Cell Compatible Arginine Containing Cationic Polymer: One-Pot Synthesis and Preliminary Biological Assessment
2014. 1st International Conference on Infectious Diseases and Nanomedicine (ICIDN). p. 59-73. DOI : 10.1007/978-81-322-1777-0_5.Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells
International journal of molecular and cellular medicine. 2014.Arginine-Based Biodegradable Ether–Ester Polymers with Low Cytotoxicity as Potential Gene Carriers
Biomacromolecules. 2014. DOI : 10.1021/bm5005977.Virus-free transient protein production in Sf9 cells
Journal Of Biotechnology. 2014. DOI : 10.1016/j.jbiotec.2013.11.018.2013
The optimization of a rapid low-cost alternative of large-scale medium sterilization
2013. 23rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health, Lille, France, 23-26 June 2013. p. P45. DOI : 10.1186/1753-6561-7-S6-P45.Study of the improved Sf9 transient gene expression process
2013. 23rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health, Lille, France, 23-26 June 2013. p. P19. DOI : 10.1186/1753-6561-7-S6-P19.Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells
Protein Expression and Purification. 2013. DOI : 10.1016/j.pep.2013.09.001.High expression of the aspartate–glutamate carrier Aralar1 favors lactate consumption in CHO cell culture
Pharmaceutical Bioprocessing. 2013. DOI : 10.4155/pbp.13.5.Disposable 600-mL orbitally shaken bioreactor for mammalian cell cultivation in suspension
Biochemical Engineering Journal. 2013. DOI : 10.1016/j.bej.2013.04.008.Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures
Processes. 2013. DOI : 10.3390/pr1010003.DNA delivery with hyperbranched polylysine: A comparative study with linear and dendritic polylysine
Journal of Controlled Release. 2013. DOI : 10.1016/j.jconrel.2013.01.019.2012
Comparative Study on the In Vitro Cytotoxicity of Linear, Dendritic, and Hyperbranched Polylysine Analogues
Biomacromolecules. 2012. DOI : 10.1021/bm300930j.A NanoDrop-based method for rapid determination of viability decline in suspension cultures of animal cells
Analytical Biochemistry. 2012. DOI : 10.1016/j.ab.2012.08.028.Hyperbranched Polylysine: A Versatile, Biodegradable Transfection Agent for the Production of Recombinant Proteins by Transient Gene Expression and the Transfection of Primary Cells
Macromolecular Bioscience. 2012. DOI : 10.1002/mabi.201100519.Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells
Biotechnology and Bioengineering. 2012. DOI : 10.1002/bit.24494.Poly(ethyleneimine)-Mediated Large-Scale Transient Gene Expression: Influence of Molecular Weight, Polydispersity and N-Propionyl Groups
Macromolecular Bioscience. 2012. DOI : 10.1002/mabi.201100404.k(L)a as a predictor for successful probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors
New biotechnology. 2012. DOI : 10.1016/j.nbt.2011.10.010.Reduced glutamine concentration improves protein production in growth-arrested CHO-DG44 and HEK-293E cells
Biotechnology letters. 2012. DOI : 10.1007/s10529-011-0809-z.2011
Hydrodynamic stress in orbitally shaken bioreactors
2011. p. P39. DOI : 10.1186/1753-6561-5-S8-P39.Transient gene expression with CHO cells in conditioned medium: a study using TubeSpin® bioreactors
2011. p. P38. DOI : 10.1186/1753-6561-5-S8-P38.Influence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells
2011. p. P35. DOI : 10.1186/1753-6561-5-S8-P35.Rapid recombinant protein production from pools of transposon-generated CHO cells
2011. p. P34. DOI : 10.1186/1753-6561-5-S8-P34.The use of filler DNA for improved transfection and reduced DNA needs in transient gene expression with CHO and HEK cells
2011. p. P33. DOI : 10.1186/1753-6561-5-S8-P33.Transposon mediated co-integration and co-expression of transgenes in CHO-DG44 cells
2011. p. P32. DOI : 10.1186/1753-6561-5-S8-P32.CHO cell lines generated by PiggyBac transposition
2011. p. P31. DOI : 10.1186/1753-6561-5-S8-P31.Large-Scale Transfection of Mammalian Cells
Protein Expression in Mammalian Cells; Humana Press, 2011. p. 13-26.The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability
Biotechnology and Bioengineering. 2011. DOI : 10.1002/bit.23167.A simple high-yielding process for transient gene expression in CHO cells
Journal of Biotechnology. 2011. DOI : 10.1016/j.jbiotec.2011.03.001.Efficient and reproducible mammalian cell bioprocesses without probes and controllers?
New biotechnology. 2011. DOI : 10.1016/j.nbt.2011.02.004.TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension
Biotechnology Letters. 2011. DOI : 10.1007/s10529-011-0527-6.2010
Innovative, Non-stirred Bioreactors in Scales from Milliliters up to 1000 Liters for Suspension Cultures of Cells using Disposable Bags and Containers – A Swiss Contribution
CHIMIA International Journal for Chemistry. 2010. DOI : 10.2533/chimia.2010.819.Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells
Journal of biotechnology. 2010. DOI : 10.1016/j.jbiotec.2010.05.003.Hyperosmolarity enhances transient recombinant protein yield in Chinese hamster ovary cells
Biotechnology Letters. 2010. DOI : 10.1007/s10529-010-0331-8.2009
Transient Gene Expression in Mammalian Cells: Promises and Challenges for Medical Biotechnology
Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology; Hoboken, N.J: Wiley, 2009.Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells
Vaccine. 2009. DOI : 10.1016/j.vaccine.2009.06.019.2008
Recombinant therapeutic protein production in cultivated mammalian cells: current status and future prospects
Drug Discovery Today: Technologies. 2008. DOI : 10.1016/j.ddtec.2008.12.003.Sustained activation and tumor targeting of NKT cells using a CD1d-anti-HER2-scFv fusion protein induce antitumor effects in mice
Journal of Clinical Investigation. 2008. DOI : 10.1172/JCI33249.2007
High-titer, serum-free production of adeno-associated virus vectors by polyethyleneimine-mediated plasmid transfection in mammalian suspension cells
Biotechnology Letters. 2007. DOI : 10.1007/s10529-007-9441-3.Recombinant protein production by large-scale transient gene expression in mammalian cells : state of the art and future perspectives
Biotechnology Letters. 2007. DOI : 10.1007/s10529-006-9297-y.Glass reflow on 3-dimensional micro-apertures for electrophysiological measurements on-chip
Microfluidics and Nanofluidics. 2007. DOI : 10.1007/s10404-006-0111-x.2005
Transient gene expression in suspension HEK-293 cells: application to large-scale protein production
Biotechnology progress. 2005. DOI : 10.1021/bp049830x.2004
Natural anti-FcepsilonRIalpha autoantibodies may interfere with diagnostic tests for autoimmune urticaria
J Autoimmun. 2004. DOI : 10.1016/j.jaut.2003.09.007.Specific recognition of the viral protein UL18 by CD85j/LIR-1/ILT2 on CD8+ T cells mediates the non-MHC-restricted lysis of human cytomegalovirus-infected cells
Journal of Immunology. 2004. DOI : 10.4049/jimmunol.172.9.5629.Reduction of adenovirus E1A mRNA by RNAi results in enhanced recombinant protein expression in transiently transfected HEK293 cells
Gene. 2004. DOI : 10.1016/j.gene.2004.06.054.2003
Transient gene expression in suspension HEK-293 cells: application to large-scale protein production
2003. p. 365-367. DOI : 10.1007/1-4020-3103-3_71.2002
Transient gene expression: A novel mammalian cell-based technology for recombinant protein production. A historical and technical perspective
2002. p. 83-88. DOI : 10.1007/978-94-017-0726-8_15.2001
Na+ is responsible for intra-run variations of specific productivity of CHO cell lines producing human anti RhD-IgG in bioreactors
2001. p. 193-196. DOI : 10.1007/978-94-010-0369-8_42.1999
The SPP biotech module 1: proteins for medical applications: a multidisciplinary effort for the development of a recombinant anti-RhD antibody preparation for the prevention of hemolytic disease of the newborn
1999. 16th ESACT Meeting, Lugano, Switzerland, Apr. 25-29, 1999. p. 455-458. DOI : 10.1007/0-306-46875-1_96.Research
All publications
The full list of my publications is available on my Publon Profile