Lucia Baldi Unser

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Project Officer

lucia.baldi@epfl.ch +41 21 693 55 62

Citizenship: Italian and Swiss

Linkedin ID

https://orcid.org/0000-0001-5550-9435

EPFL VPA-EM GE
CE 1 625 (Centre Est)
Station 1
1015 Lausanne

+41 21 693 55 62
Office: CE 1 625
EPFL > VPA-EM > VPA-EM-EM > VPA-EM-GE

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Administrative data

Fields of expertise

Management of academic organizations
Communication & events management
Gender equality promotion in academia
Past research activities:
Mammalian cells biotechnology ; Biopharmaceuticals ; Bioprocess engineering ; Non-viral gene delivery ; Stable cell lines development ; Gene expression and regulation ; Molecular immunology.

Biography

01/2021 - 04/2022 Deputy to the Associate VP for Research / Deputy to the Associate VP for Centers & Platforms, EPFL
06/2017 - 12/2020 Deputy to the Dean, School of Basic Sciences, Ecole Polytechnique Fédérale de Lausanne ;
01/2015 - 11/2017 Lecturer/Communication and outreach manager at the School of Life Sciences, EPFL
04/1998 - 03/2015: Research and Teaching Associate/Lecturer, Laboratory of Cellular Biotechnology, School of Life Sciences; 01/1994 - 07/1997: Visiting Fellow, Laboratory of Immunoregulation, NIAID, NIH, Bethesda, Maryland, USA;
1991 - 1994: Research Fellow, Laboratory of Pediatric Oncology, G. Gaslini Scientific Institute, Genova, Italy;
1987 - 1990: Research Fellow, Clinical Immunology and Immunogenetics Dept., Nat. Inst. for Cancer Research, Genova, Italy.

Education

PhD (Board Certification)

Clinical Immunology and Allergy

University of Genova, Italy, and NIAID, NIH, Bethesda MD, USA

1994

MS, Biology

Microbiology

University of Genova, Italy

1986

Publications

Infoscience publications

Publications of the last years at EPFL

2018

Improved process conditions for increasing expression of MHC class II protein from a stable Drosophila S2 cell line

X. Shen; D. Dojcinovic; L. Baldi; D. Hacker; I. Luescher et al.

BIOTECHNOLOGY LETTERS. 2018. DOI : 10.1007/s10529-017-2440-0.

Lucia Baldi Unser

Project Officer

Fields of expertise

Biography

Education

Publications

Infoscience publications

Publications of the last years at EPFL

2018

Improved process conditions for increasing expression of MHC class II protein from a stable Drosophila S2 cell line

2016

A comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation

User-Friendly Image-Based Segmentation and Analysis of Chromosomes

Comparison of Three Transposons for the Generation of Highly Productive Recombinant CHO Cell Pools and Cell Lines

Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension

2015

Enhanced Plasmid DNA Utilization in Transiently Transfected CHO-DG44 Cells in the Presence of Polar Solvents

A simple plasmid-based transient gene expression method using High Five cells

Transient Production of Recombinant Adeno-Associated Virus (AAV) Vectors for Gene Therapy Applications Using Suspension-Adapted HEK 293 Cells in Orbital Shaken Bioreactors

Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools

Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells

2014

Cell Compatible Arginine Containing Cationic Polymer: One-Pot Synthesis and Preliminary Biological Assessment

Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells

Arginine-Based Biodegradable Ether–Ester Polymers with Low Cytotoxicity as Potential Gene Carriers

Virus-free transient protein production in Sf9 cells

2013

The optimization of a rapid low-cost alternative of large-scale medium sterilization

Study of the improved Sf9 transient gene expression process

Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells

High expression of the aspartate–glutamate carrier Aralar1 favors lactate consumption in CHO cell culture

Disposable 600-mL orbitally shaken bioreactor for mammalian cell cultivation in suspension

Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures

DNA delivery with hyperbranched polylysine: A comparative study with linear and dendritic polylysine

2012

Comparative Study on the In Vitro Cytotoxicity of Linear, Dendritic, and Hyperbranched Polylysine Analogues

A NanoDrop-based method for rapid determination of viability decline in suspension cultures of animal cells

Hyperbranched Polylysine: A Versatile, Biodegradable Transfection Agent for the Production of Recombinant Proteins by Transient Gene Expression and the Transfection of Primary Cells

Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells

Poly(ethyleneimine)-Mediated Large-Scale Transient Gene Expression: Influence of Molecular Weight, Polydispersity and N-Propionyl Groups

k(L)a as a predictor for successful probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors

Reduced glutamine concentration improves protein production in growth-arrested CHO-DG44 and HEK-293E cells

2011

Hydrodynamic stress in orbitally shaken bioreactors

Transient gene expression with CHO cells in conditioned medium: a study using TubeSpin® bioreactors

Influence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells

Rapid recombinant protein production from pools of transposon-generated CHO cells

The use of filler DNA for improved transfection and reduced DNA needs in transient gene expression with CHO and HEK cells

Transposon mediated co-integration and co-expression of transgenes in CHO-DG44 cells

CHO cell lines generated by PiggyBac transposition

Large-Scale Transfection of Mammalian Cells

The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability

A simple high-yielding process for transient gene expression in CHO cells

Efficient and reproducible mammalian cell bioprocesses without probes and controllers?

TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension

2010

Innovative, Non-stirred Bioreactors in Scales from Milliliters up to 1000 Liters for Suspension Cultures of Cells using Disposable Bags and Containers – A Swiss Contribution

Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells

Hyperosmolarity enhances transient recombinant protein yield in Chinese hamster ovary cells

2009

Transient Gene Expression in Mammalian Cells: Promises and Challenges for Medical Biotechnology

Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells

2008

Recombinant therapeutic protein production in cultivated mammalian cells: current status and future prospects

Sustained activation and tumor targeting of NKT cells using a CD1d-anti-HER2-scFv fusion protein induce antitumor effects in mice

2007

High-titer, serum-free production of adeno-associated virus vectors by polyethyleneimine-mediated plasmid transfection in mammalian suspension cells

Recombinant protein production by large-scale transient gene expression in mammalian cells : state of the art and future perspectives

Glass reflow on 3-dimensional micro-apertures for electrophysiological measurements on-chip

2005

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

2004

Natural anti-FcepsilonRIalpha autoantibodies may interfere with diagnostic tests for autoimmune urticaria

Specific recognition of the viral protein UL18 by CD85j/LIR-1/ILT2 on CD8+ T cells mediates the non-MHC-restricted lysis of human cytomegalovirus-infected cells

Reduction of adenovirus E1A mRNA by RNAi results in enhanced recombinant protein expression in transiently transfected HEK293 cells

2003

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

2002

Transient gene expression: A novel mammalian cell-based technology for recombinant protein production. A historical and technical perspective

2001