Mor-Miri Mishkovsky
EPFL SB IPHYS LIFMET
CH F0 582 (Bâtiment CH)
Station 6
1015 Lausanne
+41 21 693 67 50
Office: CH F1 622
EPFL › SB › IPHYS › LIFMET
Site web: https://lifmet.epfl.ch/
Expertise
Spectroscopie par résonance magnétique in vivo (SRM)
Résonance magnétique nucléaire à haute résolution (RMN)
RMN multidimensionnelle ultra-rapide
Polarisation nucléaire dynamique (PDN)
Résonance magnétique nucléaire à haute résolution (RMN)
RMN multidimensionnelle ultra-rapide
Polarisation nucléaire dynamique (PDN)
I am a chemist, graduated Cum Laude in 2002 from Tel Aviv University in Israel. I obtained a PhD in Physical Chemistry from the Weizmann Institute of Science in Israel on the development of new technology for rapid detection of nuclear magnetic resonance (NMR) spectroscopic data aiming at reducing the measurement time from hour to a fraction of seconds. My thesis «Methodological Developments in Ultrafast Multidimensional NMR» granted me the Auto Swartz excellence award in 2007. Since 2009 I am working with the Laboratory of Functional and Metabolic Imaging at the école Polytechnique Fédérale de Lausanne (EPFL), Switzerland,
on the development of hyperpolarized (HP) magnetic resonance spectroscopy (MRS) methodologies for monitoring biochemical processes in real-time.
My research is focused on the development of Magnetic Resonance (MR) Molecular Imaging by dissolution dynamic nuclear polarization (dDNP). HP agents to interrogate biochemical processes in real-time are applied in vivo in the healthy brain and disease models, aiming at improving our understanding of cerebral function and metabolism toward better clinical diagnostics and therapy.
on the development of hyperpolarized (HP) magnetic resonance spectroscopy (MRS) methodologies for monitoring biochemical processes in real-time.
My research is focused on the development of Magnetic Resonance (MR) Molecular Imaging by dissolution dynamic nuclear polarization (dDNP). HP agents to interrogate biochemical processes in real-time are applied in vivo in the healthy brain and disease models, aiming at improving our understanding of cerebral function and metabolism toward better clinical diagnostics and therapy.
Publications représentatives
Localized in vivo hyperpolarization transfer sequences.
M. Mishkovsky, T. Cheng, A. Comment, R Gruetter
Published in Magn. Res. Med. Available on line (2011) doi: 10.1002/mrm.23231 in
Scavenging free radicals to preserve enhancement and extend relaxation times in NMR using dynamic nuclear polarization.
Mi�ville P, Ahuja P, Sarkar R, Jannin S, Vasos PR, Gerber-Lemaire S, Mishkovsky M, Comment A, Gruetter R, Ouari O, Tordo P, Bodenhausen G.
Published in Angew Chem Int Ed 49:6182-6185 (2010) in
Perfect state transfers by selective quantum interferences within complex spin networks
G. A. Alvarez, M. Mishkovsky, E. P. Danieli, P. R. Levstein, H. M. Pastawski, and L. Frydman
Published in Phys. Rev. A., Phys. Rev. A. 81:060302 (2010) in
Nearly 10(6)-fold enhancements in intermolecular (1)H double-quantum NMR experiments by nuclear hyperpolarization
M. Mishkovsky, U. Eliav, G. Navon, L. Frydman
Published in J. Magn. Reson., 200: 142-146 (2009) in
Principles and Progress in Ultrafast Multidimensional Nuclear Magnetic Resonance
M. Mishkovsky, L. Frydman
Published in Annu. Rev. Phys. Chem., 60:429-448 (2009). in
Progress in Hyperpolarized Ultrafast 2D NMR Spectroscopy
M. Mishkovsky, L. Frydman
Published in Chem. Phys. Chem., 9: 2340-2348 (2008). � Cover paper in
Spatially-Encoded Strategies and the Execution of Biomolecule-Oriented 3D NMR Experiments
M. Mishkovsky, M. Gal and L. Frydman
Published in J. Biomol. NMR., 39: 291-301, (2007). in
Ultrafast-Based Projection Reconstruction 3D Nuclear Magnetic Resonance Spectroscopy
M. Mishkovsky, E. Kupce and L. Frydman
Published in J. Chem. Phys., 127, 034507, (2007) in
Real-Time Monitoring of Chemical Transformations by Ultrafast 2D NMR Spectroscopy
M. Gal, M. Mishkovsky and L. Frydman
Published in J. Am. Chem. Soc., 128, 951-956 (2006). in
Interlaced Fourier Transformation of Ultrafast 2D NMR Data
M. Mishkovsky and L. Frydman
Published in J. Magn. Reson., 173:344-350 (2005). in
Sensitivity Enhancement in 1D Heteronuclear NMR Spectroscopy via Single-Scan Inverse Experiments
M. Mishkovsky and L. Frydman
Published in Chem. Phys. Chem, 5:779-786 (2004). in
Journal Articles
Influence of DNP Polarizing Agents on Biochemical Processes: TEMPOL in Transient Ischemic Stroke
ACS Chemical Neuroscience. 2023. DOI : 10.1021/acschemneuro.3c00137.Measuring Glycolytic Activity with Hyperpolarized [2H7, U-13C6] D-Glucose in the Naive Mouse Brain under Different Anesthetic Conditions
Metabolites. 2021-06-23. DOI : 10.3390/metabo11070413.Hyperpolarized C-13-glucose magnetic resonance highlights reduced aerobic glycolysis in vivo in infiltrative glioblastoma
Scientific Reports. 2021-03-11. DOI : 10.1038/s41598-021-85339-7.Noninvasive rapid detection of metabolic adaptation in activated human T lymphocytes by hyperpolarized 13C magnetic resonance
Scientific Reports. 2020-01-13. DOI : 10.1038/s41598-019-57026-1.Evaluating the potential of hyperpolarised [1-13C] L-lactate as a neuroprotectant metabolic biosensor for stroke
Scientific Reports. 2020. DOI : 10.1038/s41598-020-62319-x.Hyperpolarized C-13 Magnetic Resonance Spectroscopy Reveals the Rate-Limiting Role of the Blood-Brain Barrier in the Cerebral Uptake and Metabolism of L-Lactate in Vivo
Acs Chemical Neuroscience. 2018-11-01. DOI : 10.1021/acschemneuro.8b00066.Measuring glucose cerebral metabolism in the healthy mouse using hyperpolarized C-13 magnetic resonance
Scientific Reports. 2017. DOI : 10.1038/s41598-017-12086-z.Producing Radical-Free Hyperpolarized Perfusion Agents for In Vivo Magnetic Resonance Using Spin-Labeled Thermoresponsive Hydrogel
Macromolecular rapid communications. 2016. DOI : 10.1002/marc.201600133.Hyperpolarized 6Li as a probe for hemoglobin oxygenation level
Contrast Media and Molecular Imaging. 2016. DOI : 10.1002/cmmi.1656.Correcting surface coil excitation inhomogeneities in single-shot SPEN MRI
Journal of Magnetic Resonance. 2015. DOI : 10.1016/j.jmr.2015.08.018.Hyperpolarization without persistent radicals for in vivo real-time metabolic imaging
Proceedings of the National Academy of Sciences. 2013. DOI : 10.1073/pnas.1314928110.Automated transfer and injection of hyperpolarized molecules with polarization measurement prior to in vivo NMR
NMR in biomedicine. 2013. DOI : 10.1002/nbm.2993.In vivo enzymatic activity of acetylCoA synthetase in skeletal muscle revealed by (13)C turnover from hyperpolarized [1-(13)C]acetate to [1-(13)C]acetylcarnitine
Biochimica et biophysica acta. 2013. DOI : 10.1016/j.bbagen.2013.03.023.In vivo detection of brain Krebs cycle intermediate by hyperpolarized magnetic resonance
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism. 2012. DOI : 10.1038/jcbfm.2012.136.Localized in vivo hyperpolarization transfer sequences
Magnetic Resonance in Medicine. 2012. DOI : 10.1002/mrm.23231.Scavenging Free Radicals To Preserve Enhancement and Extend Relaxation Times in NMR using Dynamic Nuclear Polarization
Angewandte Chemie International Edition. 2010. DOI : 10.1002/anie.201000934.Progress in Hyperpolarized Ultrafast 2D NMR Spectroscopy
ChemPhysChem. 2008. DOI : 10.1002/cphc.200800461.Spatially encoded strategies in the execution of biomolecular-oriented 3D NMR experiments
Journal of Biomolecular NMR. 2007. DOI : 10.1007/s10858-007-9195-1.Ultrafast-based projection-reconstruction three-dimensional nuclear magnetic resonance spectroscopy
The Journal of Chemical Physics. 2007. DOI : 10.1063/1.2748768.Real-Time Monitoring of Chemical Transformations by Ultrafast 2D NMR Spectroscopy
Journal of the American Chemical Society. 2006. DOI : 10.1021/ja0564158.Interlaced Fourier transformation of ultrafast 2D NMR data
Journal of Magnetic Resonance. 2005. DOI : 10.1016/j.jmr.2005.01.007.Sensitivity Enhancement in 1D Heteronuclear NMR Spectroscopy via Single-Scan Inverse Experiments
ChemPhysChem. 2004. DOI : 10.1002/cphc.200400005.Conference Papers
In vivo Hyperpolarized 13C MRS using DPPH as polarizing agent
2014. 1st Joint Turkish-German Symposium on Human Neuroscience, Berlin, Germany, June 5-7, 2014.In vivo Hyperpolarized 13C MRS using DPPH as polarizing agent
2014. Swiss Physical Society Annual Meeting 2014, Fribourg, Switzerland, June 30-July2, 2014.In vivo Hyperpolarized 13C MRS using DPPH as polarizing agent
2014. International Society for Magnetic Resonance in Medicine, Milano, Italy, 2014.Studying glioblastoma progression in a rat model of human glioma initiating cells using 1H MRS and DTI
2014. International Society for Magnetic Resonance in Medicine, Milano, Italy, 2014.Purely endogenous hyperpolarized [1-13C]Pyruvate solutions for metabolic study in glioblastoma rat models
2014. International Society for Magnetic Resonance in Medicine, Milano, Italy, 2014.Bioreactor to simultaneously measure three parallel in vitro preparations
2013. 4th International DNP Symposium, Copenhagen, Denmark, August 28-31, 2013.Automated protocol for hyperpolarized in vitro and in vivo 13C MRS using a hydrophobic radical.
2013. 4th International DNP Symposium, Copenhagen, Denmark, August 28-31, 2013.Brain metabolism in rat model of human glioma initiating cells
2013. International Society for Magnetic Resonance in Medicine, Salt Lake City, USA, April 2013.Hyperpolarized 6Li as probe for hemoglobin oxygenation level
2012. ESMRMB 29th Annual Scientific Meeting, Lisbon, Portugal, October 4-6, 2012.Method to minimize the polarization losses in hyperpolarized biomolecules prior to in vivo MR experiments
2012. ESMRMB 29th Annual Scientific Meeting, Lisbon, October 4-6, 2012.Producing radical-free hyperpolarized solutions for in vivo magnetic resonance
2012. ISMRM 20th Annual Scientific Meeting, Melbourne, Australia, May 5 -11, 2012. p. 1664.Kinetic analysis of acetylCoA synthetase activity in skeletal muscle
2012. ESMRMB 29th Annual Scientific Meeting, Lisbon, Portugal, October 4-6, 2012..In vivo enzymatic assay of carnitine acetyl transferase and acetylCoA synthetase using hyperpolarized acetate
2012. ISMRM, Melbourne, May 2012. p. 4327.BDPA as a polarizing agent for in vivo hyperpolarized MR experiment
2012. Metaflux Meeting, May 2012.Effects of Blood and Plasma Oxygenation on the Spin Relaxation Times of Hyperpolarized 6Li
2011. Joint Annual Meeting - Swiss Physical Society, Lausanne CH, 15-17/06/2011.Localized in vivo hyperpolarization transfer experiments
2011. 19th Annual ISMRM Meeting, Montreal, Canada, May 7-13, 2011. p. 3530.In vivo detection of brain Krebs cycle intermediate by hyperpolarized MR
2011. 19th Annual ISMRM Meeting, Montreal, Canada, May 7-13, 2011.. p. 660.In situ polarization measurement of hyperpolarized solutions prior to in vivo 9.4T MR experiments
2011. 18th Annual ISMRM Meeting, Montreal, Canada, May 7-13, 2011.. p. 1504.In vivo real-time metabolic studies of mice brain and skeletal muscle at 9.4T
2011. Third International Symposium on Dynamic Nuclear Polarization, Lausanne, September 2011.The metabolic effect of hyperpolarized [1-13C]acetate dosage in vivo
2011. Third International Symposium on Dynamic Nuclear Polarization, Lausanne, September 2011.Study of acetylcarnitine kinetics in skeletal muscle in vivo using hyperpolarized 1-13C acetate
2011. ISMRM, Montreal, May 2011. p. 3536.Hyperpolarized acetate as a metabolic tracer for skeletal and cardiac muscle energetics
2011. Hot Topics in Molecular Imaging - TOPIM, Les Houches, January 2011.In vivo localized 15N MRS detection of hyperpolarized 15N labeled choline in the rat brain
2011. ISMRM 19th Annual Scientific Meeting, Montreal, Canada, 7-13 May 2011.In vivo hyperpolarized 89Y studies in a 9.4T animal scanner
2010. 18th Annual ISMRM Meeting, May 2008., Stockholm, Sweden, May 1-7, 2010. p. 1030.Reviews
Hyperpolarized MRS: New tool to study real-time brain function and metabolism
Analytical biochemistry. 2017. DOI : 10.1016/j.ab.2016.09.020.Principles and Progress in Ultrafast Multidimensional Nuclear Magnetic Resonance
Annual Review of Physical Chemistry. 2009. DOI : 10.1146/annurev.physchem.040808.090420.Posters
Hyperpolarization of 2-keto[1-13C]isocaproate for in vivo studies with photo-induced radicals
ISMRM 2017, Honolulu, HI, USA, April 22-27, 2017.Lactate brain metabolism in different mouse strains probed by hyperpolarized 13C MRS
4th International DNP Symposium, Copenhagen, Denmark, August 28-31, 2013.In vivo real-time metabolic studies in mice at physiological concentrations following 1-13C lactate injection
ISMRM 20th annual meeting, Merbourne, Australia, May 5 - 11, 2012.Journal Articles
Influence of DNP Polarizing Agents on Biochemical Processes: TEMPOL in Transient Ischemic Stroke
ACS Chemical Neuroscience. 2023. DOI : 10.1021/acschemneuro.3c00137.Measuring Glycolytic Activity with Hyperpolarized [2H7, U-13C6] D-Glucose in the Naive Mouse Brain under Different Anesthetic Conditions
Metabolites. 2021-06-23. DOI : 10.3390/metabo11070413.Hyperpolarized C-13-glucose magnetic resonance highlights reduced aerobic glycolysis in vivo in infiltrative glioblastoma
Scientific Reports. 2021-03-11. DOI : 10.1038/s41598-021-85339-7.Noninvasive rapid detection of metabolic adaptation in activated human T lymphocytes by hyperpolarized 13C magnetic resonance
Scientific Reports. 2020-01-13. DOI : 10.1038/s41598-019-57026-1.Evaluating the potential of hyperpolarised [1-13C] L-lactate as a neuroprotectant metabolic biosensor for stroke
Scientific Reports. 2020. DOI : 10.1038/s41598-020-62319-x.Hyperpolarized C-13 Magnetic Resonance Spectroscopy Reveals the Rate-Limiting Role of the Blood-Brain Barrier in the Cerebral Uptake and Metabolism of L-Lactate in Vivo
Acs Chemical Neuroscience. 2018-11-01. DOI : 10.1021/acschemneuro.8b00066.Measuring glucose cerebral metabolism in the healthy mouse using hyperpolarized C-13 magnetic resonance
Scientific Reports. 2017. DOI : 10.1038/s41598-017-12086-z.Producing Radical-Free Hyperpolarized Perfusion Agents for In Vivo Magnetic Resonance Using Spin-Labeled Thermoresponsive Hydrogel
Macromolecular rapid communications. 2016. DOI : 10.1002/marc.201600133.Hyperpolarized 6Li as a probe for hemoglobin oxygenation level
Contrast Media and Molecular Imaging. 2016. DOI : 10.1002/cmmi.1656.Correcting surface coil excitation inhomogeneities in single-shot SPEN MRI
Journal of Magnetic Resonance. 2015. DOI : 10.1016/j.jmr.2015.08.018.Hyperpolarization without persistent radicals for in vivo real-time metabolic imaging
Proceedings of the National Academy of Sciences. 2013. DOI : 10.1073/pnas.1314928110.Automated transfer and injection of hyperpolarized molecules with polarization measurement prior to in vivo NMR
NMR in biomedicine. 2013. DOI : 10.1002/nbm.2993.In vivo enzymatic activity of acetylCoA synthetase in skeletal muscle revealed by (13)C turnover from hyperpolarized [1-(13)C]acetate to [1-(13)C]acetylcarnitine
Biochimica et biophysica acta. 2013. DOI : 10.1016/j.bbagen.2013.03.023.In vivo detection of brain Krebs cycle intermediate by hyperpolarized magnetic resonance
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism. 2012. DOI : 10.1038/jcbfm.2012.136.Localized in vivo hyperpolarization transfer sequences
Magnetic Resonance in Medicine. 2012. DOI : 10.1002/mrm.23231.Scavenging Free Radicals To Preserve Enhancement and Extend Relaxation Times in NMR using Dynamic Nuclear Polarization
Angewandte Chemie International Edition. 2010. DOI : 10.1002/anie.201000934.Progress in Hyperpolarized Ultrafast 2D NMR Spectroscopy
ChemPhysChem. 2008. DOI : 10.1002/cphc.200800461.Spatially encoded strategies in the execution of biomolecular-oriented 3D NMR experiments
Journal of Biomolecular NMR. 2007. DOI : 10.1007/s10858-007-9195-1.Ultrafast-based projection-reconstruction three-dimensional nuclear magnetic resonance spectroscopy
The Journal of Chemical Physics. 2007. DOI : 10.1063/1.2748768.Real-Time Monitoring of Chemical Transformations by Ultrafast 2D NMR Spectroscopy
Journal of the American Chemical Society. 2006. DOI : 10.1021/ja0564158.Interlaced Fourier transformation of ultrafast 2D NMR data
Journal of Magnetic Resonance. 2005. DOI : 10.1016/j.jmr.2005.01.007.Sensitivity Enhancement in 1D Heteronuclear NMR Spectroscopy via Single-Scan Inverse Experiments
ChemPhysChem. 2004. DOI : 10.1002/cphc.200400005.Conference Papers
In vivo Hyperpolarized 13C MRS using DPPH as polarizing agent
2014. 1st Joint Turkish-German Symposium on Human Neuroscience, Berlin, Germany, June 5-7, 2014.In vivo Hyperpolarized 13C MRS using DPPH as polarizing agent
2014. Swiss Physical Society Annual Meeting 2014, Fribourg, Switzerland, June 30-July2, 2014.In vivo Hyperpolarized 13C MRS using DPPH as polarizing agent
2014. International Society for Magnetic Resonance in Medicine, Milano, Italy, 2014.Studying glioblastoma progression in a rat model of human glioma initiating cells using 1H MRS and DTI
2014. International Society for Magnetic Resonance in Medicine, Milano, Italy, 2014.Purely endogenous hyperpolarized [1-13C]Pyruvate solutions for metabolic study in glioblastoma rat models
2014. International Society for Magnetic Resonance in Medicine, Milano, Italy, 2014.Bioreactor to simultaneously measure three parallel in vitro preparations
2013. 4th International DNP Symposium, Copenhagen, Denmark, August 28-31, 2013.Automated protocol for hyperpolarized in vitro and in vivo 13C MRS using a hydrophobic radical.
2013. 4th International DNP Symposium, Copenhagen, Denmark, August 28-31, 2013.Brain metabolism in rat model of human glioma initiating cells
2013. International Society for Magnetic Resonance in Medicine, Salt Lake City, USA, April 2013.Hyperpolarized 6Li as probe for hemoglobin oxygenation level
2012. ESMRMB 29th Annual Scientific Meeting, Lisbon, Portugal, October 4-6, 2012.Method to minimize the polarization losses in hyperpolarized biomolecules prior to in vivo MR experiments
2012. ESMRMB 29th Annual Scientific Meeting, Lisbon, October 4-6, 2012.Producing radical-free hyperpolarized solutions for in vivo magnetic resonance
2012. ISMRM 20th Annual Scientific Meeting, Melbourne, Australia, May 5 -11, 2012. p. 1664.Kinetic analysis of acetylCoA synthetase activity in skeletal muscle
2012. ESMRMB 29th Annual Scientific Meeting, Lisbon, Portugal, October 4-6, 2012..In vivo enzymatic assay of carnitine acetyl transferase and acetylCoA synthetase using hyperpolarized acetate
2012. ISMRM, Melbourne, May 2012. p. 4327.BDPA as a polarizing agent for in vivo hyperpolarized MR experiment
2012. Metaflux Meeting, May 2012.Effects of Blood and Plasma Oxygenation on the Spin Relaxation Times of Hyperpolarized 6Li
2011. Joint Annual Meeting - Swiss Physical Society, Lausanne CH, 15-17/06/2011.Localized in vivo hyperpolarization transfer experiments
2011. 19th Annual ISMRM Meeting, Montreal, Canada, May 7-13, 2011. p. 3530.In vivo detection of brain Krebs cycle intermediate by hyperpolarized MR
2011. 19th Annual ISMRM Meeting, Montreal, Canada, May 7-13, 2011.. p. 660.In situ polarization measurement of hyperpolarized solutions prior to in vivo 9.4T MR experiments
2011. 18th Annual ISMRM Meeting, Montreal, Canada, May 7-13, 2011.. p. 1504.In vivo real-time metabolic studies of mice brain and skeletal muscle at 9.4T
2011. Third International Symposium on Dynamic Nuclear Polarization, Lausanne, September 2011.The metabolic effect of hyperpolarized [1-13C]acetate dosage in vivo
2011. Third International Symposium on Dynamic Nuclear Polarization, Lausanne, September 2011.Study of acetylcarnitine kinetics in skeletal muscle in vivo using hyperpolarized 1-13C acetate
2011. ISMRM, Montreal, May 2011. p. 3536.Hyperpolarized acetate as a metabolic tracer for skeletal and cardiac muscle energetics
2011. Hot Topics in Molecular Imaging - TOPIM, Les Houches, January 2011.In vivo localized 15N MRS detection of hyperpolarized 15N labeled choline in the rat brain
2011. ISMRM 19th Annual Scientific Meeting, Montreal, Canada, 7-13 May 2011.In vivo hyperpolarized 89Y studies in a 9.4T animal scanner
2010. 18th Annual ISMRM Meeting, May 2008., Stockholm, Sweden, May 1-7, 2010. p. 1030.Reviews
Hyperpolarized MRS: New tool to study real-time brain function and metabolism
Analytical biochemistry. 2017. DOI : 10.1016/j.ab.2016.09.020.Principles and Progress in Ultrafast Multidimensional Nuclear Magnetic Resonance
Annual Review of Physical Chemistry. 2009. DOI : 10.1146/annurev.physchem.040808.090420.Posters
Hyperpolarization of 2-keto[1-13C]isocaproate for in vivo studies with photo-induced radicals
ISMRM 2017, Honolulu, HI, USA, April 22-27, 2017.Lactate brain metabolism in different mouse strains probed by hyperpolarized 13C MRS
4th International DNP Symposium, Copenhagen, Denmark, August 28-31, 2013.In vivo real-time metabolic studies in mice at physiological concentrations following 1-13C lactate injection
ISMRM 20th annual meeting, Merbourne, Australia, May 5 - 11, 2012.Enseignement et PhD
Cours
Advanced biomedical imaging methods and instrumentation
PHYS-719
The main goal of this course is to give the student a solid introduction into approaches, methods, and instrumentation used in biomedical research. A major focus is on Magnetic Resonance Imaging (MRI) and related methods, but other imaging modalities will be increasingly covered.