Niels Lion
EPFL ENT-R CNP
Campus Biotech B3
Ch. des Mines 9
CH-1202 Genève
+41 21 693 18 42
Office: B3 2 222.134
EPFL > ENT-R > CNP > CNP-GE
Web site: Web site: https://cnp.epfl.ch/
Fields of expertise
Bioanalytics
Blood industry
R&D management
Innovation
Technology transfer
Blood industry
R&D management
Innovation
Technology transfer
Biography
With an initial training in industrial biotechnologies and biomedical engineering, I specialized in analytical biochemistry in the laboratory of Prof. Hubert Girault (PhD and postdoc, EPFL-ISIC-LEPA). I then contributed to several SNSF, and European projects, as well as collaborations with the industry (research contracts and CTI projects), all related to sensor development for various life sciences or medical topics (largely protein analysis and proteomics, food allergies).I then joined the blood industry, first to develop an independent R&D program, that proved to be highly successful in terms of product launches, scientific publications, and international recognition. I also rapidly took operational duties, such as the supervision of production, and quality control activities, which gave me a solid experience of innovation and routine management in highly regulated environments (Swissmedic, GMP, ISO...).
Being a major actor of technology transfer in the Swiss blood industry, I was appointed as national head of the workgroup responsible for market release recommandations for all new blood products or production material.
Being the lead author of more than 70 publications, co-inventor of four patents, I am at the benefit of a broad experience in bringing innovation to the real world, both from academic and industrial perspectives.
I also had the chance to be appointed as external lecturer at EPFL to teach advanced bioanalytics and sensor design to chemistry and microtechnology master students.
Publications
Infoscience publications
Journal Articles
2008
Microparticles in stored red blood cells: an approach using flow cytometry and proteomic tools, in Vox Sanguinis, vol. 95, p. 288-297, 2008.
Microfabricated Dual Sprayer for On-Line Mass Tagging of Phosphopeptides, in Anal. Chem., vol. 80, p. 2531-2538, 2008.
Application of proteomics to hematology: the revolution is starting, in Expert Review of Proteomics, vol. 5, num. 3, p. 375-379, 2008.
Electrospray Micromixer Chip for On-Line Derivatization and Kinetic Studies, in Analytical Chemistry, vol. 80, p. 3372-3378, 2008.
Electrokinetic supercharging for highly efficient peptide preconcentration in capillary zone electrophoresis, in Electrophoresis, vol. 29, num. 7, p. 1565-1572, 2008.
Oxidation of proteins: basic principles and perspectives for blood proteomics, in Proteomics-Clinical Applications, vol. 2, num. 2, p. 142-157, 2008.
2007
Capillary Electrophoresis as a Second Dimension to Isoelectric Focusing for Peptide Separation, in Analytical Chemistry, vol. 79, num. 15, p. 5949-5955, 2007.
Modeling the Isoelectric Focusing of Peptides in an OFFGEL Multicompartment Cell, in Journal of Proteome Research, vol. 6, num. 5, p. 1666-1676, 2007.
Detection of proteins on membranes and in microchannels using copper staining combined with Scanning Electrochemical Microscopy, in Journal of Electroanalytical Chemistry, vol. 599, num. 2, p. 349-355, 2007.
2006
Multitrack electrospray chips, in Journal of Mass Spectrometry, vol. 41, num. 11, p. 1484-1490, 2006.
2005
SPS’ Digest: the Swiss Proteomics Society selection of proteomics articles, in Proteomics, vol. 5, num. 12, p. 3045-3047, 2005.
Copper staining/labeling and scanning electrochemical microscopy readout of proteins on poly(vinylidene difluoride) membranes, in Chimia, vol. 59, num. 3, p. 105-108, 2005.
On-Line Counting of Cysteine Residues in Peptides during Electrospray Ionization by Electrogenerated Tags and Their Application to Protein Identification, in Electrophoresis, vol. 26, num. 1, p. 238, 2005.
Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis, in Lab on a Chip, vol. 5, num. 3, p. 298, 2005.
Salt Removal During Off-GelTM Electrophoresis of Protein Samples, in Electrophoresis, vol. 26, num. 9, p. 1650, 2005.
Editorial: Microfluidics in system biology, in Electrophoresis, vol. 26, num. 19, p. 3593, 2005.
Chip electrospray mass spectrometry for carbohydrate analysis, in Electrophoresis, vol. 26, num. 19, p. 3650, 2005.
2004
Why the move to microfluidics for protein analysis?, in Current Opinion in Biotechnology, vol. 15, num. 1, p. 31, 2004.
Electrochemical and theoretical aspects of electrospray ionisation, in Physical Chemistry Chemical Physics, vol. 6, num. 12, p. 3056, 2004.
Flow-Rate Characterization of Microfabricated Polymer Microspray Emitters, in Rapid Communications in Mass Spectrometry, vol. 18, num. 14, p. 1614, 2004.
A Thin Chip Microsprayer System Coupled to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Glycopeptide Screening, in Rapid Communications in Mass Spectrometry, vol. 18, num. 23, p. 2913, 2004.
Detection of Proteins on Poly(vinylidene difluoride) membranes by Scanning Electrochemical Microscopy, in Electrochemistry Communications, vol. 6, num. 12, p. 1217, 2004.
Generation of Mass Tags by the Inherent Electrochemistry of Electrospray for Protein Mass Spectrometry, in Journal of the American Society for Mass Spectrometry, vol. 15, num. 12, p. 1767, 2004.
2003
Thin-Chip Microspray System for High-Performance Fourier-Transform Ion-Cyclotron Resonance Mass Spectrometry of Biopolymers, in Angewandte Chemie, International Edition, vol. 42, num. 1, p. 53, 2003.
On-chip protein sample desalting and preparation for direct coupling with electrospray ionization mass spectrometry, in Journal of Chromatography A, vol. 1003, num. 1-2, p. 11, 2003.
Microfluidic systems in proteomics, in Electrophoresis, vol. 24, num. 21, p. 3533, 2003.
2002
Integration of a membrane-based desalting step in a microfabricated disposable polymer injector for mass spectrometric protein analysis, in Electrophoresis, vol. 23, num. 20, p. 3583, 2002.
Books
2006
. Microfluidic Applications in Biology From Technologies to Systems Biology. Wiley-VCH, Weinheim, 3-527-31761-9, 2006.
PhD Theses
2006
. Microfluidic front-end technologies for protein electrospray mass spectrometry. EPFL, Lausanne, 2006.
Patents
2008
Plate for matrix-assisted laser desorption ionization mass spectrometry for, e.g. analyzing peptide, has substrate that is covered with light sensitive matrix with light absorber, charge carrier, probe molecule and photo-sensitizer, US8080784, 2008.
2002
Apparatus and method for dispensing a sample, US7098450, 2002.
Infoscience
Plate for matrix-assisted laser desorption ionization mass spectrometry for, e.g. analyzing peptide, has substrate that is covered with light sensitive matrix with light absorber, charge carrier, probe molecule and photo-sensitizer, US8080784, 2008.
Microparticles in stored red blood cells: an approach using flow cytometry and proteomic tools, in Vox Sanguinis, vol. 95, p. 288-297, 2008.
Microfabricated Dual Sprayer for On-Line Mass Tagging of Phosphopeptides, in Anal. Chem., vol. 80, p. 2531-2538, 2008.
Application of proteomics to hematology: the revolution is starting, in Expert Review of Proteomics, vol. 5, num. 3, p. 375-379, 2008.
Electrospray Micromixer Chip for On-Line Derivatization and Kinetic Studies, in Analytical Chemistry, vol. 80, p. 3372-3378, 2008.
Electrokinetic supercharging for highly efficient peptide preconcentration in capillary zone electrophoresis, in Electrophoresis, vol. 29, num. 7, p. 1565-1572, 2008.
Oxidation of proteins: basic principles and perspectives for blood proteomics, in Proteomics-Clinical Applications, vol. 2, num. 2, p. 142-157, 2008.
Capillary Electrophoresis as a Second Dimension to Isoelectric Focusing for Peptide Separation, in Analytical Chemistry, vol. 79, num. 15, p. 5949-5955, 2007.
Modeling the Isoelectric Focusing of Peptides in an OFFGEL Multicompartment Cell, in Journal of Proteome Research, vol. 6, num. 5, p. 1666-1676, 2007.
Detection of proteins on membranes and in microchannels using copper staining combined with Scanning Electrochemical Microscopy, in Journal of Electroanalytical Chemistry, vol. 599, num. 2, p. 349-355, 2007.
Multitrack electrospray chips, in Journal of Mass Spectrometry, vol. 41, num. 11, p. 1484-1490, 2006.
. Microfluidic Applications in Biology From Technologies to Systems Biology. Wiley-VCH, Weinheim, 3-527-31761-9, 2006.
. Microfluidic front-end technologies for protein electrospray mass spectrometry. EPFL, Lausanne, 2006.
SPS’ Digest: the Swiss Proteomics Society selection of proteomics articles, in Proteomics, vol. 5, num. 12, p. 3045-3047, 2005.
Copper staining/labeling and scanning electrochemical microscopy readout of proteins on poly(vinylidene difluoride) membranes, in Chimia, vol. 59, num. 3, p. 105-108, 2005.
On-Line Counting of Cysteine Residues in Peptides during Electrospray Ionization by Electrogenerated Tags and Their Application to Protein Identification, in Electrophoresis, vol. 26, num. 1, p. 238, 2005.
Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis, in Lab on a Chip, vol. 5, num. 3, p. 298, 2005.
Salt Removal During Off-GelTM Electrophoresis of Protein Samples, in Electrophoresis, vol. 26, num. 9, p. 1650, 2005.
Editorial: Microfluidics in system biology, in Electrophoresis, vol. 26, num. 19, p. 3593, 2005.
Chip electrospray mass spectrometry for carbohydrate analysis, in Electrophoresis, vol. 26, num. 19, p. 3650, 2005.
Why the move to microfluidics for protein analysis?, in Current Opinion in Biotechnology, vol. 15, num. 1, p. 31, 2004.
Electrochemical and theoretical aspects of electrospray ionisation, in Physical Chemistry Chemical Physics, vol. 6, num. 12, p. 3056, 2004.
Flow-Rate Characterization of Microfabricated Polymer Microspray Emitters, in Rapid Communications in Mass Spectrometry, vol. 18, num. 14, p. 1614, 2004.
A Thin Chip Microsprayer System Coupled to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Glycopeptide Screening, in Rapid Communications in Mass Spectrometry, vol. 18, num. 23, p. 2913, 2004.
Detection of Proteins on Poly(vinylidene difluoride) membranes by Scanning Electrochemical Microscopy, in Electrochemistry Communications, vol. 6, num. 12, p. 1217, 2004.
Generation of Mass Tags by the Inherent Electrochemistry of Electrospray for Protein Mass Spectrometry, in Journal of the American Society for Mass Spectrometry, vol. 15, num. 12, p. 1767, 2004.
Thin-Chip Microspray System for High-Performance Fourier-Transform Ion-Cyclotron Resonance Mass Spectrometry of Biopolymers, in Angewandte Chemie, International Edition, vol. 42, num. 1, p. 53, 2003.
On-chip protein sample desalting and preparation for direct coupling with electrospray ionization mass spectrometry, in Journal of Chromatography A, vol. 1003, num. 1-2, p. 11, 2003.
Microfluidic systems in proteomics, in Electrophoresis, vol. 24, num. 21, p. 3533, 2003.
Apparatus and method for dispensing a sample, US7098450, 2002.
Integration of a membrane-based desalting step in a microfabricated disposable polymer injector for mass spectrometric protein analysis, in Electrophoresis, vol. 23, num. 20, p. 3583, 2002.
Selected publications
| Arnaud IL, Josserand J, Jensen H, Lion N, Roussel C, Girault HH Electrophoresis, 26(9): 1650-1658, 2005 |
Salt removal during Off-GelTM electrophoresis of protein samples |
| Zamfir AD, Lion N, Vukelic Z, Bindila L, Rossier JS, Girault HH, Peter-Katalinic J Lab on a Chip, 5(3), 298-307, 2005 |
Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis |
| Carano M, Lion N, Girault HH Chimia, 59(3): 105-108, 2005 |
Copper staining/labeling and scanning electrochemical microscopy readout of proteins on poly(vinylidene difluoride) membranes |
| Dayon L, Roussel C, Prudent M, Lion N, Girault HH Electrophoresis, 26(1): 238-247, 2005 |
On-line counting of cysteine residues in peptides during electrospray ionization by electrogenerated tags and their application to protein identificatioN |
| Roussel C, Dayon L, Rohner TC, Josserand J, Rossier JS, Jensen H, Girault HH Journal of the American Society for Mass Spectrometry, 15(12): 1767-1779, 2004 |
Generation of mass tags by the inherent electrochemistry of electrospray for protein mass spectrometry |
| Carano M, Lion N, Abid JP, Girault HH Electrochemistry Communications, 6(12): 1217-1221, 2004 |
Detection of proteins on poly(vinylidene difluoride) membranes by scanning electrochemical microscopy |
| Bindila L, Froesch M, Lion N, Vukelic Z, Rossier JS, Girault HH, Peter-Katalinic J, Zamfir AD Rapid Communications in Mass Spectrometry, 18(23): 2004 |
A thin chip microsprayer system coupled to Fourier transform ion cyclotron resonance mass spectrometry for glycopeptide screening |
| Roussel C, Dayon L, Rohner TC, Lion N, Girault HH Proceedings of SPIE -- Volume 5461, Biophotonics New Frontier: From Genome to Proteome, Michel D. Faupel, Patrick Meyrueis, Editors, September 2004, pp. 30-37 |
On-line electrochemical tagging of free cysteines during nanospray ionisation for mass spectrometry analysis |
| Lion N, Gellon JO, Girault HH Rapid Communications in Mass Spectrometry, 18(4): 1614-1620, 2004 |
Flow-rate characterization of microfabricated polymer microspray emitters |
| Rohner TC, Lion N, Girault HH Physical Chemistry Chemical Physics, 6(12): 3056-3068, 2004 |
Electrochemical and theoretical aspects of electrospray ionization (invited article) |
| Lion N, Reymond F, Girault HH, Rossier JS Current Opinion in Biotechnology, 15(1): 31-37, 2004 |
Why the move to microfluidics for protein analysis? |
| Lion N, Rohner TC, Dayon L, Arnaud IL, Damoc E, Youhnovski N, Roussel C, Josserand J, Jensen H, Rossier JS, Przybylski M, Girault HH Electrophoresis, 24(21): 3533-3562, 2003 |
Microfluidic systems in proteomics |
| Rossier JS, Youhnovski N, Lion N, Damoc N, Reymond F, Girault HH, Przybylski M Angewandte Chemie International Edition, 42(1): 53-58, 2003 |
Thin-chip Microspray system for high-performance Fourier-transform ion cyclotron resonance mass spectrometry of biopolymers |
| Lion N, Gellon JO, Jensen H, Girault HH Journal of Chromatography A, 1003(1-2): 11-19, 2003 |
On-chip protein sample desalting and preparation for direct coupling with electrospray ionization mass spectrometry |
| Lion N, Gobry V, Jensen H, Rossier JS, Girault HH Electrophoresis, 23(20): 3583-3588, 2002 |
Integration of a membrane-based desalting step in a microfabricated disposable polymer injector for mass spectrometric protein analysis |
| Rossier JS, Reymond F, Girault HH, Rohner TC, Lion N, Gobry V patent, WO 02/080222 A1, 2002 |
Apparatus for dispensing a sample |