Priya Ramakrishna
EPFL ENAC IIE PAL
GR C2 525 (Bâtiment GR)
Station 2
1015 Lausanne
+41 21 693 42 43
+41 21 693 80 39
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| Priya Ramakrishna, Francisco M. Gámez-Arjona, Etienne Bellani, Cristina Martin-Olmos, Stéphane Escrig, Damien De Bellis, Anna De Luca, José M. Pardo, Francisco J. Quintero, Christel Genoud, Clara Sánchez-Rodriguez, Niko Geldner & Anders Meibom Increasing soil salinity causes significant crop losses globally; therefore, understanding plant responses to salt (sodium) stress is of high importance. Plants avoid sodium toxicity through subcellular compartmentation by intricate processes involving a high level of elemental interdependence. Here we used the newly developed cryo nanoscale secondary ion mass spectrometry ion microprobe1, which allows high-resolution elemental imaging of cryo-preserved samples and reveals the subcellular distributions of key macronutrients and micronutrients in root meristem cells of Arabidopsis and rice. We found an unexpected, concentration-dependent change in sodium distribution, switching from sodium accumulation in the cell walls at low external sodium concentrations to vacuolar accumulation at stressful concentrations. We conclude that, in root meristems, a key function of the NHX family sodium/proton antiporter SALT OVERLY SENSITIVE 1 (also known as Na /H exchanger 7; SOS1/NHX7) is to sequester sodium into vacuoles, rather than extrusion of sodium into the extracellular space. |
Elemental cryo-imaging reveals SOS1-dependent vacuolar sodium accumulation |