Ruud Hovius
EPFL SB ISIC LCBM
CH B3 424 (Bâtiment CH)
Station 6
CH-1015 Lausanne
Unité: SCGC-ENS
Mission
=> Teaching: Lecture courses, tutorials and practicals in biochemistry, biophysical chemistry of membranes and signalling processes. Participating to the Commission d'Enseignement and Commission de l'Ecole Doctorale of Chemistry and Chemical Engineering.
=> Research interests: Protein function and structure, Membrane protein (especially receptors and channels) expression, structure and function, biomolecular interaction, labeling with biophysical probes, fluorescence imaging and assay and sensor techniques, single molecule techniques.
BSc course EPFL : TP Biochem_Biophys
Practical introducing 6th semester Chemistry Bachelor students in genetic engineering, protein expression and purification, and biochemical and biophysical charaterisation of proteins.
MSc course EPFL : Cellular Signalling
Masters course on advanced concepts of various cellular signalling pathways and their interactions
Graduate course : ESONN - Grenoble
Module "Advanced biophysics for microsystems design" à European School on Nanosciences & Nanotechnologies (ESONN.fr) à Grenoble, organisé par IPNG, UJF, CNRS et CEA.
Compétences
Protein structure, expression & purification
Molecular interaction & receptor activation
Fluorescence imaging & spectroscopy
Signalling & sensing
Publications
Infoscience
r. hovius : A chemogenetic approach for optical monitoring of voltage in neurons. 2018-12-20. DOI : 10.1002/anie.201812967.
E. Bozkurt; M. A. S. Perez; R. Hovius; N. J. Browning; U. Rothlisberger : A genetic algorithm based design and experimental characterization of a highly thermostable metalloprotein; Journal of the American Chemical Society. 2018. DOI : 10.1021/jacs.7b10660.
G. Hassaïne; C. Deluz; L. Grasso; R. Wyss; R. Hovius et al. : Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins: Case Study of the Mouse 5-HT3 Receptor; Membrane Protein Structure and Function Characterization; New York, NY: Springer New York, 2017. p. 139-168.
S. Scarabelli; K. T. Tan; R. Griss; R. Hovius; P. L. D’Alessandro et al. : Evaluating Cellular Drug Uptake with Fluorescent Sensor Proteins; ACS Sensors. 2017. DOI : 10.1021/acssensors.7b00331.
H. Haruki; R. Hovius; M. G. Pedersen; K. Johnsson : Tetrahydrobiopterin Biosynthesis as a Potential Target of the Kynurenine Pathway Metabolite Xanthurenic Acid; Journal Of Biological Chemistry. 2015. DOI : 10.1074/jbc.C115.680488.
E. Bozkurt; R. Hovius; T. A. Soares; U. Rothlisberger : Biomimetic Sequestration of CO2: reprogramming the B1 domain of protein g through a combined computational and experimental approach. 2015. 29th Annual Symposium of the Protein Society, July 22-25, 2015, Barcelona, Spain. p. 178. DOI : 10.1002/pro.2823.
A. Latremoliere; A. Latini; N. Andrews; S. Cronin; M. Fujita et al. : Reduction of Neuropathic and Inflammatory Pain through Inhibition of the Tetrahydrobiopterin Pathway; Neuron. 2015. DOI : 10.1016/j.neuron.2015.05.033.
M. Werner; R. Palankar; L. Arm; R. Hovius; H. Vogel : Microfluidic Single-Cell Analysis with Affinity Beads; Small. 2015. DOI : 10.1002/smll.201402650.
D. Andres : Characterization and scale-up of an aqueous two-phase extraction ; 2014.
P. Lederrey : Sensory characterization of perfumery raw materials and perfumes: individual sensitivity, trigeminal components and attentional effect ; 2014.
S. Chen; R. Gopalakrishnan; T. Schaer; F. Marger; R. Hovius et al. : Dithiol amino acids can structurally shape and enhance the ligand-binding properties of polypeptides; Nature Chemistry. 2014. DOI : 10.1038/nchem.2043.
G. Hassaine; C. Deluz; L. Grasso; R. Wyss; M. B. Tol et al. : X-ray structure of the mouse serotonin 5-HT3 receptor; Nature. 2014. DOI : 10.1038/nature13552.
J. Walstab; C. Wohlfarth; R. Hovius; S. Schmitteckert; R. Röth et al. : Natural compounds boldine and menthol are antagonists of human 5-HT; Neurogastroenterology & Motility. 2014. DOI : 10.1111/nmo.12334.
C. Bonnaud; C. A. Monnier; D. Demurtas; C. Jud; D. Vanhecke et al. : Insertion of Nanoparticle Clusters into Vesicle Bilayers; ACS Nano. 2014. DOI : 10.1021/nn406349z.
E. Prifti; L. Reymond; M. Umebayashi; R. Hovius; H. Riezman et al. : A fluorogenic probe for SNAP-tagged plasma membrane proteins based on the solvatochromic molecule Nile Red; ACS Chemical Biology. 2014. DOI : 10.1021/cb400819c.
A. Delachat : Towards reversible self-folding droplet networks ; 2013.
M. Werner; L. Arm; R. Palankar; R. Hovius; H. Vogel : Microfluidic single cell analysis with affinity beads. 2013. 9th European-Biophysical-Societies-Association Congress. p. S114-S114.
L. Grasso; R. Wyss; J. Piguet; M. Werner; G. Hassaïne et al. : Downscaling the Analysis of Complex Transmembrane Signaling Cascades to Closed Attoliter Volumes; PLoS ONE. 2013. DOI : 10.1371/journal.pone.0070929.
R. Hovius : Characterization and Validation of Fluorescent Receptor Ligands: A Case Study of the Ionotropic Serotonin Receptor; Methods in Molecular Biology Chemical Neurobiology. 2013. DOI : 10.1007/978-1-62703-345-9_12.
L. Grasso; R. Wyss; J. Piguet; M. Werner; G. Hassaïne et al. : Cell-derived plasma membrane vesicles as minimal cells for analyzing transmembrane signaling ; 56th Biophysical Society Meeting, San Diego, California, USA, February 25-29, 2012.
C. Jeanneret : Detection of allergenic proteins in wine and influence of different oenological practices on their presence ; 2011.
S. Roizard; C. Danelon; G. Hassaïne; J. Piguet; K. Schulze et al. : Activation of G-Protein-Coupled Receptors in Cell-Derived Plasma Membranes Supported on Porous Beads; Journal of the American Chemical Society. 2011. DOI : 10.1021/ja205302g.
R. Schmauder; D. Kosanic; R. Hovius; H. Vogel : Correlated Optical and Electrical Single-Molecule Measurements Reveal Conformational Diffusion from Ligand Binding to Channel Gating in the Nicotinic Acetylcholine Receptor; ChemBioChem. 2011. DOI : 10.1002/cbic.201100302.
J. Piguet; C. Schreiter; J.-M. Segura; H. Vogel; R. Hovius : Acetylcholine Receptor Organization in Membrane Domains in Muscle Cells: EVIDENCE FOR RAPSYN-INDEPENDENT AND RAPSYN-DEPENDENT MECHANISMS; Journal of Biological Chemistry. 2011. DOI : 10.1074/jbc.M110.139782.
J. Piguet / H. Vogel; R. Hovius (Dir.) : Advanced Fluorescence Microscopy to Study Plasma Membrane Protein Dynamics. Lausanne, EPFL, 2010. DOI : 10.5075/epfl-thesis-4869.
J. Kapeller; D. Möller; F. Lasitschka; F. Autschbach; R. Hovius et al. : Serotonin receptor diversity in the human colon: Expression of serotonin type 3 receptor subunits 5-HT3C, 5-HT3D, and 5-HT3E; The Journal of Comparative Neurology. 2010. DOI : 10.1002/cne.22525.
L. WICHT : Guanine nucleotide binding proteins expression and purification for biophysical studies ; 2009.
Y. Scheidegger : Expression et purification de protéines membranaires dans E.Coli et in vitro ; 2008.
Y. Gawad : Optimisation de la solubilisation du récepteur NK1 et étude de l'interaction avec la substance P par méthodes spéctrométriques et optiques ; 2008.
J. Grandl; R. Hovius; H. Vogel; D. Bertrand; E. Sakr et al. ; New conjugate of agonist ligand directed to ligand-gated ion channels of cysteine loop-family with chromophore, useful, e.g. in receptor proteins/their interaction with agonistic ligand and to study the effect of unlabelled ligands. WO2007057428 . 2007.
C. Danelon; J. Grandl; R. Hovius; H. Vogel : Modulation of proton-induced current fluctuations in the human nicotinic acetylcholine receptor channel; Biochim. Biophys. Acta. 2007.
E. G. Guignet; J.-M. Segura; R. Hovius; H. Vogel : Repetitive Reversible Labeling of Proteins at Polyhistidine Sequences for Single-Molecule Imaging in Live Cells; ChemPhysChem. 2007. DOI : 10.1002/cphc.200700065.
J. Grandl; E. Sakr; F. Kotzyba-Hibert; F. Krieger; S. Bertrand et al. : Fluorescent Epibatidine Agonists for Neuronal and Muscle-Type Nicotinic Acetylcholine Receptors; Angewandte Chemie, International Edition. 2007. DOI : 10.1002/anie.200604807.
C. Schreiter; J.-M. Segura; H. Vogel; R. Hovius; K. Martinez et al. ; Determining a receptor-ligand interaction useful in e.g. drug screening involves repetitive application of reversibly binding fluorescent ligands with fast association-dissociation kinetics. WO2006105985 . 2006.
R. Hovius; E. Guignet; B. Meyer; H. Vogel : Fluorescent Labelling of Membrane Proteins in Living Cells; Structural Genomics on Membrane Pro; CRC Press - Francis & Taylor/St Lucie Pr, 2006. p. 199-210.
J. Grandl; C. Danelon; R. Hovius; H. Vogel : Functional asymmetry of transmembrane segments in nicotinic acetylcholine receptors; European Biophysics Journal. 2006. DOI : 10.1007/s00249-006-0078-2.
B. H. Meyer; K. L. Martinez; J.-M. Segura; P. Pascoal; R. Hovius et al. : Covalent labeling of cell-surface proteins for in-vivo FRET studies; FEBS Letters. 2006. DOI : 10.1016/j.febslet.2006.02.007.
H. Meyer Bruno; J.-M. Segura; L. Martinez Karen; R. Hovius; N. George et al. : FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells; Proceedings of the National Academy of Sciences of the United States of America. 2006. DOI : 10.1073/pnas.0507686103.
H. Pick; E. L. Schmid; A.-P. Tairi; E. Ilegems; R. Hovius et al. : Investigating cellular signaling reactions in single attoliter vesicles; Journal of the American Chemical Society. 2005. DOI : 10.1021/ja044605x.
I. Geissbuehler; R. Hovius; K. L. Martinez; M. Adrian; K. R. Thampi et al. : Lipid-coated nanocrystals as multifunctionalized luminescent scaffolds for supramolecular biological assemblies; Angewandte Chemie, International Edition. 2005. DOI : 10.1002/anie.200461491.
C. Schreiter; M. Gjoni; R. Hovius; K. L. Martinez; J.-M. Segura et al. : Reversible sequential-binding probe receptor-ligand interactions in single cells; ChemBioChem. 2005. DOI : 10.1002/cbic.200500216.
G. Gopalakrishnan; J.-M. Segura; D. Stamou; C. Gaillard; M. Gjoni et al. : Synthesis of nanoscopic optical fibers using lipid membranes as templates; Angewandte Chemie, International Edition. 2005. DOI : 10.1002/anie.200500386.
M. Baeumle; D. Stamou; J.-M. Segura; R. Hovius; H. Vogel : Highly Fluorescent Streptavidin-Coated CdSe Nanoparticles: Preparation in Water, Characterization, and Micropatterning; Langmuir. 2004.
E. G. Guignet; R. Hovius; H. Vogel : Reversible site-selective labeling of membrane proteins in live cells; Nature Biotechnology. 2004. DOI : 10.1038/nbt954.
S. Milon; R. Hovius; H. Vogel; T. Wohland : Factors influencing fluorescence correlation spectroscopy measurements on membranes: simulations and experiments; Chemical Physics. 2003. DOI : 10.1016/S0301-0104(03)00018-1.
C. Schreiter; R. Hovius; M. Costioli; H. Pick; S. Kellenberger et al. : Characterization of the Ligand-binding Site of the Serotonin 5-HT3 Receptor: The Role of Glutamate Residues 97, 224,and 235; Journal of Biological Chemistry. 2003. DOI : 10.1074/jbc.M301801200.
P. Rigler; W.-P. Ulrich; R. Hovius; E. Ilegems; H. Pick et al. : Downscaling Fourier Transform Infrared Spectroscopy to the Micrometer and Nanogram Scale: Secondary Structure of Serotonin and Acetylcholine Receptors; Biochemistry. 2003. DOI : 10.1021/bi035113k.
K. L. Martinez; B. H. Meyer; R. Hovius; K. Lundstrom; H. Vogel : Ligand Binding to G Protein-Coupled Receptors in Tethered Cell Membranes; Langmuir. 2003. DOI : 10.1021/la035354t.
H. Pick; A. K. Preuss; M. Mayer; T. Wohland; R. Hovius et al. : Monitoring expression and clustering of the ionotropic 5HT3 receptor in plasma membranes of live biological cells; Biochemistry. 2003. DOI : 10.1021/bi026576d.
E. Guignet : Marquage réversible de protéines avec His-tag par NTA-chromophore, suivi par transfert d’ énergie ; 2001.
T. Wohland; H. Pick; A. Preuss; R. Hovius; H. Vogel : Characterization of a transmembrane receptor protein by fluorescence correlation spectroscopy. 2001. p. PHYS-339.
P. Vallotton; R. Hovius; H. Pick; H. Vogel : In vitro and in vivo ligand binding to the 5HT3 serotonin receptor characterised by time-resolved fluorescence spectroscopy; ChemBioChem. 2001. DOI : 10.1002/1439-7633(20010302)2:3<205::AID-CBIC205>3.0.CO;2-J.
P. Vallotton; A.-P. Tairi; T. Wohland; K. Friedrich-Benet; H. Pick et al. : Mapping the Antagonist Binding Site of the Serotonin Type 3 Receptor by Fluorescence Resonance Energy Transfer; Biochemistry. 2001. DOI : 10.1021/bi0106989.
a. Sevin-Landais; P. Rigler; s. Tzartos; f. Hucho; R. Hovius et al. : Functional immobilisation of the nicotinic acetylcholine receptor in tethered lipid membranes; Biophysical chemistry. 2000. DOI : 10.1016/S0301-4622(00)00114-9.
H. D. Blasey; B. Brethon; R. Hovius; H. Vogel H.; A. P. Tairi et al. : Large scale transient 5-HT3 receptor production with the Semliki Forest Virus Expression System; Cytotechnology. 2000. DOI : 10.1023/A:1008192709549.
R. Hovius; P. Vallotton; T. Wohland; H. Vogel : Fluorescence techniques: shedding light on ligand-receptor interactions; Trends in pharmacological sciences. 2000.
H. D. Blasey; R. Hovius; H. Vogel; A. R. Bernard : Transient-expression technologies, their application and scale-up: 5-HT3 serotonin receptor case study; Biochemical Society transactions. 2000.
T. Wohland; K. Friedrich; R. Hovius; H. Vogel : Study of ligand-receptor interactions by fluorescence correlation spectroscopy with different fluorophores: Evidence that the homopentameric 5-hydroxytryptamine type 3(As) receptor binds only one ligand; BIOCHEMISTRY. 1999. DOI : 10.1021/bi990366s.
H. D. Blasey; R. Hovius; H. Vogel; A. Bernard : Transient-expression technologies, their application and scale-up: 5-HT3 serotonin receptor case study. 1999. Colloquium on Expression and Purification of Membrane Proteins,, Keele, UK, JULy 20-22, 1999. p. 956-960.
Z. Liron; A. Bromberg; M. Fisher; E. Schmid Osborne; R. Hovius et al. : Waveguide fluorosensor for the detection of ligand-receptor interactions - The 5-HT3 serotonin receptor. 1999. 43rd OHOLO Conference on Novel Applroaches in Biosensors and Rapid Diagnostic Assays, Elat, Isareal, 1999. p. 135-145.
R. Hovius; E. L. Schmid; A.-P. Tairi; H. Blasey; A. R. Bernard et al. : Fluorescence techniques for fundamental and applied studies of membrane protein receptors: the 5-HT3 serotonin receptor; Journal of Receptor and Signal Transduction Research. 1999.
R. Hovius; A.-P. Tairi; H. Blasey; A. Bernard; K. Lundstrom et al. : Characterization of a mouse serotonin 5-HT3 receptor purified from mammalian cells; Journal of Neurochemistry. 1998.
A.-P. Tairi; R. Hovius; H. Pick; H. Blasey; A. Bernard et al. : Ligand binding to the serotonin 5HT3 receptor studied with a novel fluorescent ligand; Biochemistry. 1998. DOI : 10.1021/bi981812z.
E. L. Schmid; A.-P. Tairi; R. Hovius; H. Vogel : Screening Ligands for Membrane Protein Receptors by Total Internal Reflectance Fluorescence: The 5-HT3 Serotonin Receptor; Analytical Chemistry. 1998. DOI : 10.1021/ac9712658.
T. Stora; R. Hovius; Z. Dienes; M. Pachoud; H. Vogel : Metal ion trace detection by a chelator-modified gold electrode: A comparison of surface to bulk affinity; Langmuir. 1997. DOI : 10.1021/la970504j.
K. Lundstrom; A. Michel; H. Blasey; A. R. Bernard; R. Hovius et al. : Expression of ligand-gated ion channels with the Semliki Forest virus expression system; Journal of Receptor and Signal Transduction Research. 1997.
K. Lundstrom; H. Blasey; R. Hovius; H. Vogel; A. Bernard : The Semliki Forest virus system: receptor expression and scale-up; Genetic Engineer and Biotechnologist. 1997.
R. Hovius; J. Thijssen; P. van der Linden; K. Nicolay; B. de Kruijff : Phospholipid asymmetry of the outer membrane of rat liver mitochondria: Evidence for the presence of cardiolipin on the outside of the outer membrane; FEBS letters. 1993. DOI : 10.1016/0014-5793(93)80922-H.
R. Hovius; B. Faber; B. Brigot; K. Nicolay; B. de Kruijff : On the mechanism of the mitochondrial decarboxylation of phosphatidylserine; The Journal of biological chemistry. 1992.
M. Rojo; R. Hovius; R. A. Demel; K. Nicolay; T. Wallimann : Mitochondrial creatine kinase mediates contact formation between mitochondrial membranes; The Journal of biological chemistry. 1991.
M. Rojo; R. Hovius; R. Demel; T. Wallimann; H. M. Eppenberger et al. : Interaction of mitochondrial creatine kinase with model membranes: A monolayer study; FEBS letters. 1991. DOI : 10.1016/0014-5793(91)80374-C.
K. Nicolay; M. Rojo; T. Wallimann; R. Demel; R. Hovius : The role of contact sites between inner and outer mitochondrial membrane in energy transfer; Biochimica et biophysica acta. 1990.
K. Nicolay; R. Hovius; R. Bron; K. Wirtz; B. de Kruijff : The phosphatidylcholine-transfer protein catalyzed import of phosphatidylcholine into isolated rat liver mitochondria; Biochimica et biophysica acta. 1990. DOI : 10.1016/0005-2736(90)90189-U.
R. Hovius; H. Lambrechts; K. Nicolay; B. de Kruijff : Improved methods to isolate and subfractionate rat liver mitochondria. Lipid composition of the inner and outer membrane.; Biochimica et biophysica acta. 1990. DOI : 10.1016/0005-2736(90)90036-N.
R. A. Demel; W. Jordi; H. Lambrechts; H. van Damme; R. Hovius et al. : Differential interactions of apo- and holocytochrome c with acidic membrane lipids in model systems and the implications for their import into mitochondria; The Journal of biological chemistry. 1989.
Enseignement & Phd
Enseignement
- Chemistry and Chemical Engineering,
Programmes doctoraux
- Doctoral Program in Chemistry and Chemical Engineering
Doctorants
Cours
Cellular signalling
Présentation de chemins de signalisation en soulignant le mécanisme d'actions des molécules impliquées, les intéractions moléculaires et le rôle de leur organisation spatio-temporel dans la cellulle, en considérant les dimensions et lesconditions cellulai... 
Chemical biology
Ce cours montrera comment les outils de la chimie peuvent être utilisés pour traiter des problèmes importants de la biologie. Par la discussion d'articles de première importance en chimie biologique, les étudiants seront familiarisés avec la recherche à l...
Experimental biochemistry and biophysics
During a semester long experiment students plan and perform the construction from DNA bricks of a fluorescent sensor
protein that will be expressed and purified for characterization by biochemical and spectroscopic methods. A report in the
style of a scie...
Molecular and cellular biophysics II
Recherche
Current interests
Structure and function of proteinsReal-time monitoring of structural changes in receptor proteins and molecular interactions of signalling cascades and scaffolds
Single molecule techniques