Ruud Hovius
EPFL SB ISIC LCBM
CH B3 424 (Bâtiment CH)
Station 6
CH-1015 Lausanne
Web site: Web site: https://lcbm.epfl.ch/
Web site: Web site: https://www.epfl.ch/schools/sb/scgc/
Fields of expertise
Protein structure, expression & purification
Molecular interaction & receptor activation
Fluorescence imaging & spectroscopy
Signalling & sensing
Mission
=> Teaching: Lecture courses, tutorials and practicals in biochemistry, biophysical chemistry of membranes and signalling processes. Participating to the Commission d'Enseignement and Commission de l'Ecole Doctorale of Chemistry and Chemical Engineering. => Research interests: Protein function and structure, Membrane protein (especially receptors and channels) expression, structure and function, biomolecular interaction, labeling with biophysical probes, fluorescence imaging and assay and sensor techniques, single molecule techniques.BSc course EPFL : Practical BioChem & BioPhys
Practical introducing 6th Chemistry Bachelor students in genetic engineering, protein expression and purification, and biochemical and biophysical charaterisation of proteins.BSc course Molecular and cellular biophysics II
.MSc course : Cellular signalling
Masters course on advanced concepts of various cellular signalling pathways and their interactionsMSc Course Chemical Biology
.Graduate course : ESONN - Grenoble
Module "Advanced biophysics for microsystems design" at the post-graduate European School on Nanosciences & Nanotechnologies (ESONN.fr) in Grenoble, organized by the IPNG, UJF, CNRS and CEA.Publications
Infoscience publications
Infoscience
A covalently linked probe to monitor local membrane properties surrounding plasma membrane proteins
Journal Of Cell Biology. 2022-12-26. DOI : 10.1083/jcb.202206119.International Union of Basic and Clinical Pharmacology. CX. Classification of Receptors for 5-hydroxytryptamine; Pharmacology and Function
Pharmacological Reviews. 2021-01-01. DOI : 10.1124/pr.118.015552.Torsin ATPases influence chromatin interaction of the Torsin regulator LAP1
Elife. 2020-12-15. DOI : 10.7554/eLife.63614.Structural mechanism of cGAS inhibition by the nucleosome
Nature. 2020. DOI : 10.1038/s41586-020-2750-6.Chromatin Fiber Invasion and Nucleosome Displacement by the Rap1 Transcription Factor
Molecular Cell. 2020. DOI : 10.1016/j.molcel.2019.10.025.A Chemogenetic Approach for the Optical Monitoring of Voltage in Neurons
Angewandte Chemie International Edition. 2018-12-20. DOI : 10.1002/anie.201812967.A genetic algorithm based design and experimental characterization of a highly thermostable metalloprotein
Journal of the American Chemical Society. 2018. DOI : 10.1021/jacs.7b10660.Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins: Case Study of the Mouse 5-HT3 Receptor
Membrane Protein Structure and Function Characterization; New York, NY: Springer New York, 2017. p. 139-168.Evaluating Cellular Drug Uptake with Fluorescent Sensor Proteins
ACS Sensors. 2017. DOI : 10.1021/acssensors.7b00331.Tetrahydrobiopterin Biosynthesis as a Potential Target of the Kynurenine Pathway Metabolite Xanthurenic Acid
Journal Of Biological Chemistry. 2015. DOI : 10.1074/jbc.C115.680488.Biomimetic Sequestration of CO2: reprogramming the B1 domain of protein g through a combined computational and experimental approach
2015. 29th Annual Symposium of the Protein Society, July 22-25, 2015, Barcelona, Spain. p. 178. DOI : 10.1002/pro.2823.Reduction of Neuropathic and Inflammatory Pain through Inhibition of the Tetrahydrobiopterin Pathway
Neuron. 2015. DOI : 10.1016/j.neuron.2015.05.033.Microfluidic Single-Cell Analysis with Affinity Beads
Small. 2015. DOI : 10.1002/smll.201402650.Characterization and scale-up of an aqueous two-phase extraction
2014Sensory characterization of perfumery raw materials and perfumes: individual sensitivity, trigeminal components and attentional effect
2014Dithiol amino acids can structurally shape and enhance the ligand-binding properties of polypeptides
Nature Chemistry. 2014. DOI : 10.1038/nchem.2043.X-ray structure of the mouse serotonin 5-HT3 receptor
Nature. 2014. DOI : 10.1038/nature13552.Natural compounds boldine and menthol are antagonists of human 5-HT
Neurogastroenterology & Motility. 2014. DOI : 10.1111/nmo.12334.Insertion of Nanoparticle Clusters into Vesicle Bilayers
ACS Nano. 2014. DOI : 10.1021/nn406349z.A fluorogenic probe for SNAP-tagged plasma membrane proteins based on the solvatochromic molecule Nile Red
ACS Chemical Biology. 2014. DOI : 10.1021/cb400819c.Towards reversible self-folding droplet networks
2013Microfluidic single cell analysis with affinity beads
2013. 9th European-Biophysical-Societies-Association Congress. p. S114-S114.Downscaling the Analysis of Complex Transmembrane Signaling Cascades to Closed Attoliter Volumes
PLoS ONE. 2013. DOI : 10.1371/journal.pone.0070929.Characterization and Validation of Fluorescent Receptor Ligands: A Case Study of the Ionotropic Serotonin Receptor
Methods in Molecular Biology Chemical Neurobiology. 2013. DOI : 10.1007/978-1-62703-345-9_12.Cell-derived plasma membrane vesicles as minimal cells for analyzing transmembrane signaling
56th Biophysical Society Meeting, San Diego, California, USA, February 25-29, 2012.Detection of allergenic proteins in wine and influence of different oenological practices on their presence
2011Activation of G-Protein-Coupled Receptors in Cell-Derived Plasma Membranes Supported on Porous Beads
Journal of the American Chemical Society. 2011. DOI : 10.1021/ja205302g.Correlated Optical and Electrical Single-Molecule Measurements Reveal Conformational Diffusion from Ligand Binding to Channel Gating in the Nicotinic Acetylcholine Receptor
ChemBioChem. 2011. DOI : 10.1002/cbic.201100302.Acetylcholine Receptor Organization in Membrane Domains in Muscle Cells: EVIDENCE FOR RAPSYN-INDEPENDENT AND RAPSYN-DEPENDENT MECHANISMS
Journal of Biological Chemistry. 2011. DOI : 10.1074/jbc.M110.139782.Advanced Fluorescence Microscopy to Study Plasma Membrane Protein Dynamics
Lausanne, EPFL, 2010. DOI : 10.5075/epfl-thesis-4869.Serotonin receptor diversity in the human colon: Expression of serotonin type 3 receptor subunits 5-HT3C, 5-HT3D, and 5-HT3E
The Journal of Comparative Neurology. 2010. DOI : 10.1002/cne.22525.Guanine nucleotide binding proteins expression and purification for biophysical studies
2009Expression et purification de protéines membranaires dans E.Coli et in vitro
2008Optimisation de la solubilisation du récepteur NK1 et étude de l'interaction avec la substance P par méthodes spéctrométriques et optiques
2008New conjugate of agonist ligand directed to ligand-gated ion channels of cysteine loop-family with chromophore, useful, e.g. in receptor proteins/their interaction with agonistic ligand and to study the effect of unlabelled ligands
WO2007057428 . 2007.Modulation of proton-induced current fluctuations in the human nicotinic acetylcholine receptor channel
Biochimica et Biophysica Acta. 2007. DOI : 10.1016/j.bbamem.2006.07.015.Repetitive Reversible Labeling of Proteins at Polyhistidine Sequences for Single-Molecule Imaging in Live Cells
ChemPhysChem. 2007. DOI : 10.1002/cphc.200700065.Fluorescent Epibatidine Agonists for Neuronal and Muscle-Type Nicotinic Acetylcholine Receptors
Angewandte Chemie, International Edition. 2007. DOI : 10.1002/anie.200604807.Determining a receptor-ligand interaction useful in e.g. drug screening involves repetitive application of reversibly binding fluorescent ligands with fast association-dissociation kinetics
WO2006105985 . 2006.Fluorescent Labelling of Membrane Proteins in Living Cells
Structural Genomics on Membrane Pro; CRC Press - Francis & Taylor/St Lucie Pr, 2006. p. 199-210.Functional asymmetry of transmembrane segments in nicotinic acetylcholine receptors
European Biophysics Journal. 2006. DOI : 10.1007/s00249-006-0078-2.Covalent labeling of cell-surface proteins for in-vivo FRET studies
FEBS Letters. 2006. DOI : 10.1016/j.febslet.2006.02.007.FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells
Proceedings of the National Academy of Sciences of the United States of America. 2006. DOI : 10.1073/pnas.0507686103.Investigating cellular signaling reactions in single attoliter vesicles
Journal of the American Chemical Society. 2005. DOI : 10.1021/ja044605x.Lipid-coated nanocrystals as multifunctionalized luminescent scaffolds for supramolecular biological assemblies
Angewandte Chemie, International Edition. 2005. DOI : 10.1002/anie.200461491.Reversible sequential-binding probe receptor-ligand interactions in single cells
ChemBioChem. 2005. DOI : 10.1002/cbic.200500216.Synthesis of nanoscopic optical fibers using lipid membranes as templates
Angewandte Chemie, International Edition. 2005. DOI : 10.1002/anie.200500386.Highly Fluorescent Streptavidin-Coated CdSe Nanoparticles: Preparation in Water, Characterization, and Micropatterning
Langmuir. 2004. DOI : 10.1021/la0363940.Reversible site-selective labeling of membrane proteins in live cells
Nature Biotechnology. 2004. DOI : 10.1038/nbt954.Factors influencing fluorescence correlation spectroscopy measurements on membranes: simulations and experiments
Chemical Physics. 2003. DOI : 10.1016/S0301-0104(03)00018-1.Characterization of the Ligand-binding Site of the Serotonin 5-HT3 Receptor: The Role of Glutamate Residues 97, 224,and 235
Journal of Biological Chemistry. 2003. DOI : 10.1074/jbc.M301801200.Downscaling Fourier Transform Infrared Spectroscopy to the Micrometer and Nanogram Scale: Secondary Structure of Serotonin and Acetylcholine Receptors
Biochemistry. 2003. DOI : 10.1021/bi035113k.Ligand Binding to G Protein-Coupled Receptors in Tethered Cell Membranes
Langmuir. 2003. DOI : 10.1021/la035354t.Monitoring expression and clustering of the ionotropic 5HT3 receptor in plasma membranes of live biological cells
Biochemistry. 2003. DOI : 10.1021/bi026576d.Marquage réversible de protéines avec His-tag par NTA-chromophore, suivi par transfert d’ énergie
2001Characterization of a transmembrane receptor protein by fluorescence correlation spectroscopy
2001. p. PHYS-339.In vitro and in vivo ligand binding to the 5HT3 serotonin receptor characterised by time-resolved fluorescence spectroscopy
ChemBioChem. 2001. DOI : 10.1002/1439-7633(20010302)2:3<205::AID-CBIC205>3.0.CO;2-J.Mapping the Antagonist Binding Site of the Serotonin Type 3 Receptor by Fluorescence Resonance Energy Transfer
Biochemistry. 2001. DOI : 10.1021/bi0106989.Transient-expression technologies, their application and scale-up: 5-HT3 serotonin receptor case study
2000. Colloquium on Expression and Purification of Membrane Proteins, Keele, UK, JULy 20-22, 1999. p. 956-960. DOI : 10.1042/bst0270956.Functional immobilisation of the nicotinic acetylcholine receptor in tethered lipid membranes
Biophysical chemistry. 2000. DOI : 10.1016/S0301-4622(00)00114-9.Large scale transient 5-HT3 receptor production with the Semliki Forest Virus Expression System
Cytotechnology. 2000. DOI : 10.1023/A:1008192709549.Fluorescence techniques: shedding light on ligand-receptor interactions
Trends in pharmacological sciences. 2000.Study of ligand-receptor interactions by fluorescence correlation spectroscopy with different fluorophores: Evidence that the homopentameric 5-hydroxytryptamine type 3(As) receptor binds only one ligand
BIOCHEMISTRY. 1999. DOI : 10.1021/bi990366s.Waveguide fluorosensor for the detection of ligand-receptor interactions - The 5-HT3 serotonin receptor
1999. 43rd OHOLO Conference on Novel Applroaches in Biosensors and Rapid Diagnostic Assays, Elat, Isareal, 1999. p. 135-145. DOI : 10.1007/978-1-4615-1231-8_10.Fluorescence techniques for fundamental and applied studies of membrane protein receptors: the 5-HT3 serotonin receptor
Journal of Receptor and Signal Transduction Research. 1999. DOI : 10.3109/10799899909036670.Characterization of a mouse serotonin 5-HT3 receptor purified from mammalian cells
Journal of Neurochemistry. 1998. DOI : 10.1046/j.1471-4159.1998.70020824.x.Ligand binding to the serotonin 5HT3 receptor studied with a novel fluorescent ligand
Biochemistry. 1998. DOI : 10.1021/bi981812z.Screening Ligands for Membrane Protein Receptors by Total Internal Reflectance Fluorescence: The 5-HT3 Serotonin Receptor
Analytical Chemistry. 1998. DOI : 10.1021/ac9712658.Metal ion trace detection by a chelator-modified gold electrode: A comparison of surface to bulk affinity
Langmuir. 1997. DOI : 10.1021/la970504j.Expression of ligand-gated ion channels with the Semliki Forest virus expression system
Journal of Receptor and Signal Transduction Research. 1997. DOI : 10.3109/10799899709036597.The Semliki Forest virus system: receptor expression and scale-up
Genetic Engineer and Biotechnologist. 1997.Phospholipid asymmetry of the outer membrane of rat liver mitochondria: Evidence for the presence of cardiolipin on the outside of the outer membrane
FEBS letters. 1993. DOI : 10.1016/0014-5793(93)80922-H.On the mechanism of the mitochondrial decarboxylation of phosphatidylserine
The Journal of biological chemistry. 1992. DOI : 10.1016/S0021-9258(18)41851-0.Mitochondrial creatine kinase mediates contact formation between mitochondrial membranes
The Journal of biological chemistry. 1991. DOI : 10.1016/S0021-9258(18)54921-8.Interaction of mitochondrial creatine kinase with model membranes: A monolayer study
FEBS letters. 1991. DOI : 10.1016/0014-5793(91)80374-C.The role of contact sites between inner and outer mitochondrial membrane in energy transfer
Biochimica et biophysica acta. 1990. DOI : 10.1016/0005-2728(90)90255-3.The phosphatidylcholine-transfer protein catalyzed import of phosphatidylcholine into isolated rat liver mitochondria
Biochimica et biophysica acta. 1990. DOI : 10.1016/0005-2736(90)90189-U.Improved methods to isolate and subfractionate rat liver mitochondria. Lipid composition of the inner and outer membrane.
Biochimica et biophysica acta. 1990. DOI : 10.1016/0005-2736(90)90036-N.Differential interactions of apo- and holocytochrome c with acidic membrane lipids in model systems and the implications for their import into mitochondria
The Journal of biological chemistry. 1989. DOI : 10.1016/S0021-9258(19)84950-5.Other publications
Patents
New conjugate of agonist ligand directed to ligand-gated ion channels of cysteine loop-family with chromophore, useful, e.g. in receptor proteins/their interaction with agonistic ligand and to study the effect of unlabelled ligands. J. Grandl, R. Hovius, H. Vogel, D. Bertrand, E. Sakr, F. Kotzyba-Hibert and M. Goeldner. Patent number: WO2007057428-A1 Publication date: 2007 Determining a receptor-ligand interaction useful in e.g. drug screening involves repetitive application of reversibly binding fluorescent ligands with fast association-dissociation kinetics . C. Schreiter, J.-M. Segura, H. Vogel, R. Hovius, K. Martinez and M. Gjoni Boillat. Patent number: WO2006105985-A1 Publication date: 2006Research
Current interests
Structure and function DNA-protein macromolecular complexes Real-time monitoring of structural dynamics and molecular interactions Single molecule techniques & cellular signallingTeaching & PhD
Teaching
Chemistry and Chemical Engineering
Past EPFL PhD Students
Piguet Joachim ,ESONN
Graduate course : ESONN - Grenoble Module "Advanced biophysics for microsystems design" at the post-graduate European School on Nanosciences & Nanotechnologies (ESONN.fr) in Grenoble, organized by the IPNG, UJF, CNRS and CEA.Courses
Experimental biochemistry and biophysics
A semester long experiment where you plan and construct a fluorescent sensor protein starting from DNA bricks. The protein will be expressed in and purified from bacteria, and characterized by biochemical and spectroscopic methods. Your report will be in the style of a scientific paper.
Cellular signalling
Presentation of selected signalling pathways with emphasis on both the mechanism of action of the molecules involved, molecular interactions and the role of their spatio-temporal organization within the cell, considering cellular dimensions and conditions.