Sebastian Maerkl
Professeur associé
sebastian.maerkl@epfl.ch +41 21 693 78 35 http://lbnc.epfl.ch
Nationalité : German
EPFL STI IBI-STI LBNC
BM 5130 (Bâtiment BM)
Station 17
CH-1015 Lausanne
+41 21 693 78 35
+41 21 693 11 61
Local: BM 5130
EPFL > STI > IBI-STI > LBNC
Web site: Site web: https://lbnc.epfl.ch/
+41 21 693 78 35
EPFL > SV > SV-SSV > SSV-ENS
Web site: Site web: https://sv.epfl.ch/education
Domaines de compétences
microfluidics, systems biology, cell-free synthetic biology, transcription factor biophysics / transcriptional regulation, molecular diagnostics
Publications
Publications Infoscience
Infoscience
Microfluidic iMITOMI platform to study the architecture of low-affinity transcription factor binding site clusters
Lausanne, EPFL, 2020. DOI : 10.5075/epfl-thesis-7427.High-throughput microfluidic platforms for characterizing and engineering cell-free gene regulatory circuits
Lausanne, EPFL, 2020. DOI : 10.5075/epfl-thesis-10123.A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
Journal of Visualized Experiments. 2019-10-06. DOI : 10.3791/59655.Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals
Nature Chemical Biology. 2019-05-01. DOI : 10.1038/s41589-019-0244-3.Cell-free gene-regulatory network engineering with synthetic transcription factors
Proceedings Of The National Academy Of Sciences Of The United States Of America. 2019-03-26. DOI : 10.1073/pnas.1816591116.A Simple, Robust, and Low-Cost Method To Produce the PURE Cell Free System
Acs Synthetic Biology. 2019-02-01. DOI : 10.1021/acssynbio.8b00427.Microfluidic Transfection for High-Throughput Mammalian Protein Expression
Methods in Molecular Biology. 2018-09-22. DOI : 10.1007/978-1-4939-8730-6_13.Microfluidic Module for Real-Time Generation of Complex Multimolecule Temporal Concentration Profiles
ANALYTICAL CHEMISTRY. 2018. DOI : 10.1021/acs.analchem.7b04099.Development of microfluidic tools and methods for the biochemical characterization of scFv and Cas9 affinity reagents and their applications in molecular detection
Lausanne, EPFL, 2018. DOI : 10.5075/epfl-thesis-8614.Microfluidic device for real-time formulation of reagents and their subsequent encapsulation into double emulsions
Scientific Reports. 2018. DOI : 10.1038/s41598-018-26542-x.A Microfluidic Biodisplay
Acs Synthetic Biology. 2017. DOI : 10.1021/acssynbio.7b00088.High-throughput microfluidic platforms applied to immunoassay-based diagnostics and environmental monitoring
Lausanne, EPFL, 2017. DOI : 10.5075/epfl-thesis-7903.Microfluidic platforms for high-throughput mammalian cell printing, transfection, and dosage-dependent studies
Lausanne, EPFL, 2017. DOI : 10.5075/epfl-thesis-7889.A system, device and method for multiplexed biomarker diagnostics of ultra-low volume whole blood samples
WO2017102593 . 2017.A High-Throughput Microfluidic Platform for Mammalian Cell Transfection and Culturing
Scientific Reports. 2016-03-31. DOI : 10.1038/srep23937.Single Molecule Localization and Discrimination of DNA–Protein Complexes by Controlled Translocation Through Nanocapillaries
Nano Letters. 2016. DOI : 10.1021/acs.nanolett.6b04165.GreA and GreB Enhance Expression of Escherichia coil RNA Polymerase Promoters in a Reconstituted Transcription-Translation System
Acs Synthetic Biology. 2016. DOI : 10.1021/acssynbio.6b00017.Microfluidic co-culture platform to quantify chemotaxis of primary stem cells
Lab On A Chip. 2016. DOI : 10.1039/c6lc00236f.Integrating gene synthesis and microfluidic protein analysis for rapid protein engineering
Nucleic Acids Research. 2016. DOI : 10.1093/nar/gkv1497.A Digital-Analog Microfluidic Platform for Patient-Centric Multiplexed Biomarker Diagnostics of Ultralow Volume Samples
Acs Nano. 2016. DOI : 10.1021/acsnano.5b07939.Integrating Gene Synthesis and MITOMI for Rapid Protein Engineering
Lausanne, EPFL, 2016. DOI : 10.5075/epfl-thesis-6796.Rapid cell-free forward engineering of novel genetic ring oscillators
eLife. 2015-10-02. DOI : 10.7554/eLife.09771.High-throughput nanoimmunoassay chip
US2015285794 ; EP2908949 ; WO2014060869 . 2015.Implementation and Characterization of Dynamic Genetic Networks in Vitro
Lausanne, EPFL, 2015. DOI : 10.5075/epfl-thesis-6617.Mechanically Induced Trapping of Molecular Interactions and Its Applications
Journal of laboratory automation. 2015. DOI : 10.1177/2211068215578586.A microfluidic platform for high-throughput multiplexed protein quantitation
PloS One. 2015. DOI : 10.1371/journal.pone.0117744.Automated long-term single cell analysis of Schizosaccharomyces pombe on a microfluidic microchemostat array
Lausanne, EPFL, 2014. DOI : 10.5075/epfl-thesis-6196.Two distinct promoter architectures centered on dynamic nucleosomes control ribosomal protein gene transcription
Genes & Development. 2014. DOI : 10.1101/gad.244434.114.A 1024-sample serum analyzer chip for cancer diagnostics
Lab On A Chip. 2014. DOI : 10.1039/c31c51153g.LSPR Chip for Parallel, Rapid, and Sensitive Detection of Cancer Markers in Serum
Nano Letters. 2014. DOI : 10.1021/nl500574n.Long-Term Single Cell Analysis of S. pombe on a Microfluidic Microchemostat Array
Plos One. 2014. DOI : 10.1371/journal.pone.0093466.Systematic Analysis of Yeast Gene Expression by Synthetic Promoter Libraries
Lausanne, EPFL, 2013. DOI : 10.5075/epfl-thesis-6002.Implementation of cell-free biological networks at steady state
Proceedings Of The National Academy Of Sciences Of The United States Of America. 2013. DOI : 10.1073/pnas.1311166110.Mapping the fine structure of a eukaryotic promoter input-output function
Nature Genetics. 2013. DOI : 10.1038/ng.2729.A chemostat array enables the spatio-temporal analysis of the yeast proteome
Proceedings of the National Academy of Sciences of the United States of America. 2013. DOI : 10.1073/pnas.1308265110.iSLIM: a comprehensive approach to mapping and characterizing gene regulatory networks
Nucleic Acids Research. 2013. DOI : 10.1093/nar/gks1323.Live mammalian cell arrays
Nature Methods. 2013. DOI : 10.1038/nmeth.2473.A high-throughput nanoimmunoassay chip applied to large-scale vaccine adjuvant screening
Integrative Biology. 2013. DOI : 10.1039/c3ib20263a.Multiplexed surface micropatterning of proteins with a pressure-modulated microfluidic button-membrane
Chemical Communications. 2013. DOI : 10.1039/c2cc37740c.A microfluidic live-cell imaging platform to study large collections of microbial genotypes
Lausanne, EPFL, 2013. DOI : 10.5075/epfl-thesis-5593.Generating microarrays for protein interaction studies using microfluidic devices
Lausanne, EPFL, 2013. DOI : 10.5075/epfl-thesis-5592.Topology and dynamics of the zebrafish segmentation clock core circuit
PLoS Biology. 2012. DOI : 10.1371/journal.pbio.1001364.Real-Time mRNA Measurement during an
ACS Synthetic Biology. 2012. DOI : 10.1021/sb300104f.Massively parallel measurements of molecular interaction kinetics on a microfluidic platform
Proceedings Of The National Academy Of Sciences Of The United States Of America. 2012. DOI : 10.1073/pnas.1206011109.Rapid Synthesis of Defined Eukaryotic Promoter Libraries
Acs Synthetic Biology. 2012. DOI : 10.1021/sb300045j.Probing the Informational and Regulatory Plasticity of a Transcription Factor DNA-Binding Domain
Plos Genetics. 2012. DOI : 10.1371/journal.pgen.1002614.MITOMI: A Microfluidic Platform for In Vitro Characterization of Transcription Factor–DNA Interaction
Gene Regulatory Networks. 2011. DOI : 10.1007/978-1-61779-292-2_6.A High-Throughput Microfluidic Method for Generating and Characterizing Transcription Factor Mutant Libraries
Synthetic Gene Networks. 2011. DOI : 10.1007/978-1-61779-412-4_6.Does Positive Selection Drive Transcription Factor Binding Site Turnover? A Test with Drosophila Cis-Regulatory Modules
PLoS Genetics. 2011. DOI : 10.1371/journal.pgen.1002053.A software-programmable microfluidic device for automated biology
Lab on a Chip. 2011. DOI : 10.1039/C0LC00537A.Next generation microfluidic platforms for high-throughput protein biochemistry
Current Opinion in Biotechnology. 2011. DOI : 10.1016/j.copbio.2010.08.010.Software-reconfigurable universally-applicable microfluidic platform
2010. p. 18-ANYL.Experimental strategies for studying transcription factor-DNA binding specificities
Briefings in Functional Genomics. 2010. DOI : 10.1093/bfgp/elq023.Integration of plasmonic trapping in a microfluidic environment
Optics Express. 2009. DOI : 10.1364/OE.17.006018.Experimental determination of the evolvability of a transcription factor
Proceedings of the National Academy of Sciences of the United States of America. 2009. DOI : 10.1073/pnas.0907688106.Integration column: Microfluidic high-throughput screening
Integrative Biology. 2009. DOI : 10.1039/b819762h.An in vitro microfluidic approach to generating protein-interaction networks
Nature Methods. 2009. DOI : 10.1038/nmeth.1289.Discovery of a hepatitis C target and its pharmacological inhibitors by microfluidic affinity analysis
Nature Biotechnology. 2008.A Systems Approach to Measuring the Binding Energy Landscapes of Transcription Factors
Science. 2007. DOI : 10.1126/science.1131007.Microfluidic Large-Scale Integration
Science. 2002. DOI : 10.1126/science.1076996.2020
Microfluidic iMITOMI platform to study the architecture of low-affinity transcription factor binding site clusters
Lausanne, EPFL, 2020. DOI : 10.5075/epfl-thesis-7427.High-throughput microfluidic platforms for characterizing and engineering cell-free gene regulatory circuits
Lausanne, EPFL, 2020. DOI : 10.5075/epfl-thesis-10123.2019
A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
Journal of Visualized Experiments. 2019-10-06. DOI : 10.3791/59655.Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals
Nature Chemical Biology. 2019-05-01. DOI : 10.1038/s41589-019-0244-3.Cell-free gene-regulatory network engineering with synthetic transcription factors
Proceedings Of The National Academy Of Sciences Of The United States Of America. 2019-03-26. DOI : 10.1073/pnas.1816591116.A Simple, Robust, and Low-Cost Method To Produce the PURE Cell Free System
Acs Synthetic Biology. 2019-02-01. DOI : 10.1021/acssynbio.8b00427.2018
Microfluidic Transfection for High-Throughput Mammalian Protein Expression
Methods in Molecular Biology. 2018-09-22. DOI : 10.1007/978-1-4939-8730-6_13.Microfluidic Module for Real-Time Generation of Complex Multimolecule Temporal Concentration Profiles
ANALYTICAL CHEMISTRY. 2018. DOI : 10.1021/acs.analchem.7b04099.Development of microfluidic tools and methods for the biochemical characterization of scFv and Cas9 affinity reagents and their applications in molecular detection
Lausanne, EPFL, 2018. DOI : 10.5075/epfl-thesis-8614.Microfluidic device for real-time formulation of reagents and their subsequent encapsulation into double emulsions
Scientific Reports. 2018. DOI : 10.1038/s41598-018-26542-x.2017
A Microfluidic Biodisplay
Acs Synthetic Biology. 2017. DOI : 10.1021/acssynbio.7b00088.High-throughput microfluidic platforms applied to immunoassay-based diagnostics and environmental monitoring
Lausanne, EPFL, 2017. DOI : 10.5075/epfl-thesis-7903.Microfluidic platforms for high-throughput mammalian cell printing, transfection, and dosage-dependent studies
Lausanne, EPFL, 2017. DOI : 10.5075/epfl-thesis-7889.A system, device and method for multiplexed biomarker diagnostics of ultra-low volume whole blood samples
WO2017102593 . 2017.2016
A High-Throughput Microfluidic Platform for Mammalian Cell Transfection and Culturing
Scientific Reports. 2016-03-31. DOI : 10.1038/srep23937.Single Molecule Localization and Discrimination of DNA–Protein Complexes by Controlled Translocation Through Nanocapillaries
Nano Letters. 2016. DOI : 10.1021/acs.nanolett.6b04165.GreA and GreB Enhance Expression of Escherichia coil RNA Polymerase Promoters in a Reconstituted Transcription-Translation System
Acs Synthetic Biology. 2016. DOI : 10.1021/acssynbio.6b00017.Microfluidic co-culture platform to quantify chemotaxis of primary stem cells
Lab On A Chip. 2016. DOI : 10.1039/c6lc00236f.Integrating gene synthesis and microfluidic protein analysis for rapid protein engineering
Nucleic Acids Research. 2016. DOI : 10.1093/nar/gkv1497.A Digital-Analog Microfluidic Platform for Patient-Centric Multiplexed Biomarker Diagnostics of Ultralow Volume Samples
Acs Nano. 2016. DOI : 10.1021/acsnano.5b07939.Integrating Gene Synthesis and MITOMI for Rapid Protein Engineering
Lausanne, EPFL, 2016. DOI : 10.5075/epfl-thesis-6796.2015
Rapid cell-free forward engineering of novel genetic ring oscillators
eLife. 2015-10-02. DOI : 10.7554/eLife.09771.High-throughput nanoimmunoassay chip
US2015285794 ; EP2908949 ; WO2014060869 . 2015.Implementation and Characterization of Dynamic Genetic Networks in Vitro
Lausanne, EPFL, 2015. DOI : 10.5075/epfl-thesis-6617.Mechanically Induced Trapping of Molecular Interactions and Its Applications
Journal of laboratory automation. 2015. DOI : 10.1177/2211068215578586.A microfluidic platform for high-throughput multiplexed protein quantitation
PloS One. 2015. DOI : 10.1371/journal.pone.0117744.2014
Automated long-term single cell analysis of Schizosaccharomyces pombe on a microfluidic microchemostat array
Lausanne, EPFL, 2014. DOI : 10.5075/epfl-thesis-6196.Two distinct promoter architectures centered on dynamic nucleosomes control ribosomal protein gene transcription
Genes & Development. 2014. DOI : 10.1101/gad.244434.114.A 1024-sample serum analyzer chip for cancer diagnostics
Lab On A Chip. 2014. DOI : 10.1039/c31c51153g.LSPR Chip for Parallel, Rapid, and Sensitive Detection of Cancer Markers in Serum
Nano Letters. 2014. DOI : 10.1021/nl500574n.Long-Term Single Cell Analysis of S. pombe on a Microfluidic Microchemostat Array
Plos One. 2014. DOI : 10.1371/journal.pone.0093466.2013
Systematic Analysis of Yeast Gene Expression by Synthetic Promoter Libraries
Lausanne, EPFL, 2013. DOI : 10.5075/epfl-thesis-6002.Implementation of cell-free biological networks at steady state
Proceedings Of The National Academy Of Sciences Of The United States Of America. 2013. DOI : 10.1073/pnas.1311166110.Mapping the fine structure of a eukaryotic promoter input-output function
Nature Genetics. 2013. DOI : 10.1038/ng.2729.A chemostat array enables the spatio-temporal analysis of the yeast proteome
Proceedings of the National Academy of Sciences of the United States of America. 2013. DOI : 10.1073/pnas.1308265110.iSLIM: a comprehensive approach to mapping and characterizing gene regulatory networks
Nucleic Acids Research. 2013. DOI : 10.1093/nar/gks1323.Live mammalian cell arrays
Nature Methods. 2013. DOI : 10.1038/nmeth.2473.A high-throughput nanoimmunoassay chip applied to large-scale vaccine adjuvant screening
Integrative Biology. 2013. DOI : 10.1039/c3ib20263a.Multiplexed surface micropatterning of proteins with a pressure-modulated microfluidic button-membrane
Chemical Communications. 2013. DOI : 10.1039/c2cc37740c.A microfluidic live-cell imaging platform to study large collections of microbial genotypes
Lausanne, EPFL, 2013. DOI : 10.5075/epfl-thesis-5593.Generating microarrays for protein interaction studies using microfluidic devices
Lausanne, EPFL, 2013. DOI : 10.5075/epfl-thesis-5592.2012
Topology and dynamics of the zebrafish segmentation clock core circuit
PLoS Biology. 2012. DOI : 10.1371/journal.pbio.1001364.Real-Time mRNA Measurement during an
ACS Synthetic Biology. 2012. DOI : 10.1021/sb300104f.Massively parallel measurements of molecular interaction kinetics on a microfluidic platform
Proceedings Of The National Academy Of Sciences Of The United States Of America. 2012. DOI : 10.1073/pnas.1206011109.Rapid Synthesis of Defined Eukaryotic Promoter Libraries
Acs Synthetic Biology. 2012. DOI : 10.1021/sb300045j.Probing the Informational and Regulatory Plasticity of a Transcription Factor DNA-Binding Domain
Plos Genetics. 2012. DOI : 10.1371/journal.pgen.1002614.2011
MITOMI: A Microfluidic Platform for In Vitro Characterization of Transcription Factor–DNA Interaction
Gene Regulatory Networks. 2011. DOI : 10.1007/978-1-61779-292-2_6.A High-Throughput Microfluidic Method for Generating and Characterizing Transcription Factor Mutant Libraries
Synthetic Gene Networks. 2011. DOI : 10.1007/978-1-61779-412-4_6.Does Positive Selection Drive Transcription Factor Binding Site Turnover? A Test with Drosophila Cis-Regulatory Modules
PLoS Genetics. 2011. DOI : 10.1371/journal.pgen.1002053.A software-programmable microfluidic device for automated biology
Lab on a Chip. 2011. DOI : 10.1039/C0LC00537A.Next generation microfluidic platforms for high-throughput protein biochemistry
Current Opinion in Biotechnology. 2011. DOI : 10.1016/j.copbio.2010.08.010.2010
Software-reconfigurable universally-applicable microfluidic platform
2010. p. 18-ANYL.Experimental strategies for studying transcription factor-DNA binding specificities
Briefings in Functional Genomics. 2010. DOI : 10.1093/bfgp/elq023.2009
Integration of plasmonic trapping in a microfluidic environment
Optics Express. 2009. DOI : 10.1364/OE.17.006018.Experimental determination of the evolvability of a transcription factor
Proceedings of the National Academy of Sciences of the United States of America. 2009. DOI : 10.1073/pnas.0907688106.Integration column: Microfluidic high-throughput screening
Integrative Biology. 2009. DOI : 10.1039/b819762h.An in vitro microfluidic approach to generating protein-interaction networks
Nature Methods. 2009. DOI : 10.1038/nmeth.1289.2008
Discovery of a hepatitis C target and its pharmacological inhibitors by microfluidic affinity analysis
Nature Biotechnology. 2008.2007
A Systems Approach to Measuring the Binding Energy Landscapes of Transcription Factors
Science. 2007. DOI : 10.1126/science.1131007.2002
Microfluidic Large-Scale Integration
Science. 2002. DOI : 10.1126/science.1076996.Enseignement & Phd
Enseignement
Life Sciences Engineering