Sebastian Maerkl
EPFL STI IBI-STI LBNC
BM 5130 (Bâtiment BM)
Station 17
CH-1015 Lausanne
Publications
Infoscience
K. Woodruff; S. Maerkl : Microfluidic Module for Real-Time Generation of Complex Multimolecule Temporal Concentration Profiles; ANALYTICAL CHEMISTRY. 2018. DOI : 10.1021/acs.analchem.7b04099.
E. E. Petrova / S. Maerkl (Dir.) : Development of microfluidic tools and methods for the biochemical characterization of scFv and Cas9 affinity reagents and their applications in molecular detection. Lausanne, EPFL, 2018. DOI : 10.5075/epfl-thesis-8614.
F. Volpetti; E. Petrova; S. J. Maerkl : A Microfluidic Biodisplay; Acs Synthetic Biology. 2017. DOI : 10.1021/acssynbio.7b00088.
F. Volpetti / S. Maerkl (Dir.) : High-throughput microfluidic platforms applied to immunoassay-based diagnostics and environmental monitoring. Lausanne, EPFL, 2017. DOI : 10.5075/epfl-thesis-7903.
K. P. Woodruff / S. Maerkl (Dir.) : Microfluidic platforms for high-throughput mammalian cell printing, transfection, and dosage-dependent studies. Lausanne, EPFL, 2017. DOI : 10.5075/epfl-thesis-7889.
S. J. Maerkl; F. Piraino; F. Volpetti ; A system, device and method for multiplexed biomarker diagnostics of ultra-low volume whole blood samples. WO2017102593 . 2017.
R. D. Bulushev; S. Marion; E. Petrova; S. J. Davis; S. J. Maerkl et al. : Single Molecule Localization and Discrimination of DNA–Protein Complexes by Controlled Translocation Through Nanocapillaries; Nano Letters. 2016. DOI : 10.1021/acs.nanolett.6b04165.
L. L. De Maddalena; H. Niederholtmeyer; M. Turtola; Z. N. Swank; G. A. Belogurov et al. : GreA and GreB Enhance Expression of Escherichia coil RNA Polymerase Promoters in a Reconstituted Transcription-Translation System; Acs Synthetic Biology. 2016. DOI : 10.1021/acssynbio.6b00017.
M. C. Blackburn; E. Petrova; B. E. Correia; S. J. Maerkl : Integrating gene synthesis and microfluidic protein analysis for rapid protein engineering; Nucleic Acids Research. 2016. DOI : 10.1093/nar/gkv1497.
K. Woodruff; S. J. Maerkl : A High-Throughput Microfluidic Platform for Mammalian Cell Transfection and Culturing; Scientific Reports. 2016. DOI : 10.1038/srep23937.
F. Piraino; F. Volpetti; C. Watson; S. J. Maerkl : A Digital-Analog Microfluidic Platform for Patient-Centric Multiplexed Biomarker Diagnostics of Ultralow Volume Samples; Acs Nano. 2016. DOI : 10.1021/acsnano.5b07939.
M. C. Blackburn / S. Maerkl (Dir.) : Integrating Gene Synthesis and MITOMI for Rapid Protein Engineering. Lausanne, EPFL, 2016. DOI : 10.5075/epfl-thesis-6796.
S. Maerkl; J. L. Garcia-Cordero ; High-throughput nanoimmunoassay chip. US2015285794 ; EP2908949 ; WO2014060869 . 2015.
H. M. Niederholtmeyer / S. Maerkl (Dir.) : Implementation and Characterization of Dynamic Genetic Networks in Vitro. Lausanne, EPFL, 2015. DOI : 10.5075/epfl-thesis-6617.
J. L. Garcia-Cordero; S. J. Maerkl : Mechanically Induced Trapping of Molecular Interactions and Its Applications; Journal of laboratory automation. 2015. DOI : 10.1177/2211068215578586.
F. Volpetti; J. Garcia-Cordero; S. J. Maerkl : A microfluidic platform for high-throughput multiplexed protein quantitation; PloS one. 2015. DOI : 10.1371/journal.pone.0117744.
J.-B. Nobs / S. Maerkl (Dir.) : Automated long-term single cell analysis of Schizosaccharomyces pombe on a microfluidic microchemostat array. Lausanne, EPFL, 2014. DOI : 10.5075/epfl-thesis-6196.
B. Knight; S. Kubik; B. Ghosh; M. J. Bruzzone; M. Geertz et al. : Two distinct promoter architectures centered on dynamic nucleosomes control ribosomal protein gene transcription; Genes & Development. 2014. DOI : 10.1101/gad.244434.114.
J. L. Garcia-Cordero; S. J. Maerkl : A 1024-sample serum analyzer chip for cancer diagnostics; Lab On A Chip. 2014. DOI : 10.1039/c31c51153g.
S. S. Acimovic; M. A. Ortega; V. Sanz; J. Berthelot; J. L. Garcia-Cordero et al. : LSPR Chip for Parallel, Rapid, and Sensitive Detection of Cancer Markers in Serum; Nano Letters. 2014. DOI : 10.1021/nl500574n.
J.-B. Nobs; S. J. Maerkl : Long-Term Single Cell Analysis of S. pombe on a Microfluidic Microchemostat Array; Plos One. 2014. DOI : 10.1371/journal.pone.0093466.
A. S. Rajkumar / S. Maerkl (Dir.) : Systematic Analysis of Yeast Gene Expression by Synthetic Promoter Libraries. Lausanne, EPFL, 2013. DOI : 10.5075/epfl-thesis-6002.
H. Niederholtmeyer; V. Stepanova; S. J. Maerkl : Implementation of cell-free biological networks at steady state; Proceedings Of The National Academy Of Sciences Of The United States Of America. 2013. DOI : 10.1073/pnas.1311166110.
A. S. Rajkumar; N. Denervaud; S. J. Maerkl : Mapping the fine structure of a eukaryotic promoter input-output function; Nature Genetics. 2013. DOI : 10.1038/ng.2729.
N. Dénervaud; J. Becker; R. Delgado-Gonzalo; P. Damay; A. S. Rajkumar et al. : A chemostat array enables the spatio-temporal analysis of the yeast proteome; Proceedings of the National Academy of Sciences of the United States of America. 2013. DOI : 10.1073/pnas.1308265110.
S. Rockel; M. Geertz; K. Hens; B. Deplancke; S. J. Maerkl : iSLIM: a comprehensive approach to mapping and characterizing gene regulatory networks; Nucleic Acids Research. 2013. DOI : 10.1093/nar/gks1323.
K. P. Woodruff; L. M. Fidalgo; S. Gobaa; M. Lutolf; S. Maerkl : Live mammalian cell arrays; Nature Methods. 2013. DOI : 10.1038/nmeth.2473.
J. L. Garcia-Cordero; C. Nembrini; A. Stano; J. A. Hubbell; S. J. Maerkl : A high-throughput nanoimmunoassay chip applied to large-scale vaccine adjuvant screening; Integrative Biology. 2013. DOI : 10.1039/c3ib20263a.
J. L. Garcia-Cordero; S. J. Maerkl : Multiplexed surface micropatterning of proteins with a pressure-modulated microfluidic button-membrane; Chemical Communications. 2013. DOI : 10.1039/c2cc37740c.
N. Dénervaud / S. Maerkl; F. Naef (Dir.) : A microfluidic live-cell imaging platform to study large collections of microbial genotypes. Lausanne, EPFL, 2013. DOI : 10.5075/epfl-thesis-5593.
S. Rockel / S. Maerkl (Dir.) : Generating microarrays for protein interaction studies using microfluidic devices. Lausanne, EPFL, 2013. DOI : 10.5075/epfl-thesis-5592.
C. Schröter; S. Ares; L. G. Morelli; A. Isakova; K. Hens et al. : Topology and dynamics of the zebrafish segmentation clock core circuit; PLoS Biology. 2012. DOI : 10.1371/journal.pbio.1001364.
R. K. Shultzaberger; S. J. Maerkl; J. F. Kirsch; M. B. Eisen : Probing the Informational and Regulatory Plasticity of a Transcription Factor DNA–Binding Domain; PLoS Genetics. 2012. DOI : 10.1371/journal.pgen.1002614.
H. Niederholtmeyer; L. Xu; S. J. Maerkl : Real-Time mRNA Measurement during an; ACS Synthetic Biology. 2012. DOI : 10.1021/sb300104f.
M. Geertz; D. Shore; S. J. Maerkl : Massively parallel measurements of molecular interaction kinetics on a microfluidic platform; Proceedings Of The National Academy Of Sciences Of The United States Of America. 2012. DOI : 10.1073/pnas.1206011109.
A. S. Rajkumar; S. J. Maerkl : Rapid Synthesis of Defined Eukaryotic Promoter Libraries; Acs Synthetic Biology. 2012. DOI : 10.1021/sb300045j.
R. K. Shultzaberger; S. J. Maerkl; J. F. Kirsch; M. B. Eisen : Probing the Informational and Regulatory Plasticity of a Transcription Factor DNA-Binding Domain; Plos Genetics. 2012. DOI : 10.1371/journal.pgen.1002614.
S. Rockel; M. Geertz; S. J. Maerkl : MITOMI: A Microfluidic Platform for In Vitro Characterization of Transcription Factor–DNA Interaction; Gene Regulatory Networks. 2011. DOI : 10.1007/978-1-61779-292-2_6.
M. Geertz; S. Rockel; S. J. Maerkl : A High-Throughput Microfluidic Method for Generating and Characterizing Transcription Factor Mutant Libraries; Synthetic Gene Networks. 2011. DOI : 10.1007/978-1-61779-412-4_6.
B. Z. He; A. K. Holloway; S. J. Maerkl; M. Kreitman : Does Positive Selection Drive Transcription Factor Binding Site Turnover? A Test with Drosophila Cis-Regulatory Modules; PLoS Genetics. 2011. DOI : 10.1371/journal.pgen.1002053.
F. Rabanera; L. Miguel; S. J. Maerkl : A software-programmable microfluidic device for automated biology; Lab on a Chip. 2011. DOI : 10.1039/C0LC00537A.
L. M. Fidalgo; S. Maerkl : Software-reconfigurable universally-applicable microfluidic platform. 2010. p. -.
S. J. Maerkl : Next generation microfluidic platforms for high-throughput protein biochemistry; Current Opinion in Biotechnology. 2010. DOI : 10.1016/j.copbio.2010.08.010.
M. Geertz; S. J. Maerkl : Experimental strategies for studying transcription factor-DNA binding specificities; Briefings in Functional Genomics. 2010. DOI : 10.1093/bfgp/elq023.
L. Huang; O. J. F. Martin; S. J. Maerkl : Integration of plasmonic trapping in a microfluidic environment; Optics Express. 2009. DOI : 10.1364/OE.17.006018.
S. Maerkl; S. Quake : Experimental determination of the evolvability of a transcription factor; Proceedings of the National Academy of Sciences of the United States of America. 2009. DOI : 10.1073/pnas.0907688106.
S. Maerkl : Integration column: Microfluidic high-throughput screening; Integrative Biology. 2009. DOI : 10.1039/b819762h.
D. Gerber; S. Maerkl; S. Quake : An in vitro microfluidic approach to generating protein-interaction networks; Nature Methods. 2009. DOI : 10.1038/nmeth.1289.
S. Einav; D. Gerber; P. Bryson; E. Sklan; M. Elazar et al. : Discovery of a hepatitis C target and its pharmacological inhibitors by microfluidic affinity analysis; Nature Biotechnology. 2008.
S. Maerkl; S. Quake : A Systems Approach to Measuring the Binding Energy Landscapes of Transcription Factors; Science. 2007. DOI : 10.1126/science.1131007.
T. Thorsen; S. Maerkl; S. Quake : Microfluidic Large-Scale Integration; Science. 2002. DOI : 10.1126/science.1076996.
2018
K. Woodruff; S. Maerkl : Microfluidic Module for Real-Time Generation of Complex Multimolecule Temporal Concentration Profiles; ANALYTICAL CHEMISTRY. 2018. DOI : 10.1021/acs.analchem.7b04099.
E. E. Petrova / S. Maerkl (Dir.) : Development of microfluidic tools and methods for the biochemical characterization of scFv and Cas9 affinity reagents and their applications in molecular detection. Lausanne, EPFL, 2018. DOI : 10.5075/epfl-thesis-8614.
2017
F. Volpetti; E. Petrova; S. J. Maerkl : A Microfluidic Biodisplay; Acs Synthetic Biology. 2017. DOI : 10.1021/acssynbio.7b00088.
F. Volpetti / S. Maerkl (Dir.) : High-throughput microfluidic platforms applied to immunoassay-based diagnostics and environmental monitoring. Lausanne, EPFL, 2017. DOI : 10.5075/epfl-thesis-7903.
K. P. Woodruff / S. Maerkl (Dir.) : Microfluidic platforms for high-throughput mammalian cell printing, transfection, and dosage-dependent studies. Lausanne, EPFL, 2017. DOI : 10.5075/epfl-thesis-7889.
S. J. Maerkl; F. Piraino; F. Volpetti ; A system, device and method for multiplexed biomarker diagnostics of ultra-low volume whole blood samples. WO2017102593 . 2017.
2016
R. D. Bulushev; S. Marion; E. Petrova; S. J. Davis; S. J. Maerkl et al. : Single Molecule Localization and Discrimination of DNA–Protein Complexes by Controlled Translocation Through Nanocapillaries; Nano Letters. 2016. DOI : 10.1021/acs.nanolett.6b04165.
L. L. De Maddalena; H. Niederholtmeyer; M. Turtola; Z. N. Swank; G. A. Belogurov et al. : GreA and GreB Enhance Expression of Escherichia coil RNA Polymerase Promoters in a Reconstituted Transcription-Translation System; Acs Synthetic Biology. 2016. DOI : 10.1021/acssynbio.6b00017.
M. C. Blackburn; E. Petrova; B. E. Correia; S. J. Maerkl : Integrating gene synthesis and microfluidic protein analysis for rapid protein engineering; Nucleic Acids Research. 2016. DOI : 10.1093/nar/gkv1497.
K. Woodruff; S. J. Maerkl : A High-Throughput Microfluidic Platform for Mammalian Cell Transfection and Culturing; Scientific Reports. 2016. DOI : 10.1038/srep23937.
F. Piraino; F. Volpetti; C. Watson; S. J. Maerkl : A Digital-Analog Microfluidic Platform for Patient-Centric Multiplexed Biomarker Diagnostics of Ultralow Volume Samples; Acs Nano. 2016. DOI : 10.1021/acsnano.5b07939.
M. C. Blackburn / S. Maerkl (Dir.) : Integrating Gene Synthesis and MITOMI for Rapid Protein Engineering. Lausanne, EPFL, 2016. DOI : 10.5075/epfl-thesis-6796.
2015
S. Maerkl; J. L. Garcia-Cordero ; High-throughput nanoimmunoassay chip. US2015285794 ; EP2908949 ; WO2014060869 . 2015.
H. M. Niederholtmeyer / S. Maerkl (Dir.) : Implementation and Characterization of Dynamic Genetic Networks in Vitro. Lausanne, EPFL, 2015. DOI : 10.5075/epfl-thesis-6617.
J. L. Garcia-Cordero; S. J. Maerkl : Mechanically Induced Trapping of Molecular Interactions and Its Applications; Journal of laboratory automation. 2015. DOI : 10.1177/2211068215578586.
F. Volpetti; J. Garcia-Cordero; S. J. Maerkl : A microfluidic platform for high-throughput multiplexed protein quantitation; PloS one. 2015. DOI : 10.1371/journal.pone.0117744.
2014
J.-B. Nobs / S. Maerkl (Dir.) : Automated long-term single cell analysis of Schizosaccharomyces pombe on a microfluidic microchemostat array. Lausanne, EPFL, 2014. DOI : 10.5075/epfl-thesis-6196.
B. Knight; S. Kubik; B. Ghosh; M. J. Bruzzone; M. Geertz et al. : Two distinct promoter architectures centered on dynamic nucleosomes control ribosomal protein gene transcription; Genes & Development. 2014. DOI : 10.1101/gad.244434.114.
J. L. Garcia-Cordero; S. J. Maerkl : A 1024-sample serum analyzer chip for cancer diagnostics; Lab On A Chip. 2014. DOI : 10.1039/c31c51153g.
S. S. Acimovic; M. A. Ortega; V. Sanz; J. Berthelot; J. L. Garcia-Cordero et al. : LSPR Chip for Parallel, Rapid, and Sensitive Detection of Cancer Markers in Serum; Nano Letters. 2014. DOI : 10.1021/nl500574n.
J.-B. Nobs; S. J. Maerkl : Long-Term Single Cell Analysis of S. pombe on a Microfluidic Microchemostat Array; Plos One. 2014. DOI : 10.1371/journal.pone.0093466.
2013
A. S. Rajkumar / S. Maerkl (Dir.) : Systematic Analysis of Yeast Gene Expression by Synthetic Promoter Libraries. Lausanne, EPFL, 2013. DOI : 10.5075/epfl-thesis-6002.
H. Niederholtmeyer; V. Stepanova; S. J. Maerkl : Implementation of cell-free biological networks at steady state; Proceedings Of The National Academy Of Sciences Of The United States Of America. 2013. DOI : 10.1073/pnas.1311166110.
A. S. Rajkumar; N. Denervaud; S. J. Maerkl : Mapping the fine structure of a eukaryotic promoter input-output function; Nature Genetics. 2013. DOI : 10.1038/ng.2729.
N. Dénervaud; J. Becker; R. Delgado-Gonzalo; P. Damay; A. S. Rajkumar et al. : A chemostat array enables the spatio-temporal analysis of the yeast proteome; Proceedings of the National Academy of Sciences of the United States of America. 2013. DOI : 10.1073/pnas.1308265110.
S. Rockel; M. Geertz; K. Hens; B. Deplancke; S. J. Maerkl : iSLIM: a comprehensive approach to mapping and characterizing gene regulatory networks; Nucleic Acids Research. 2013. DOI : 10.1093/nar/gks1323.
K. P. Woodruff; L. M. Fidalgo; S. Gobaa; M. Lutolf; S. Maerkl : Live mammalian cell arrays; Nature Methods. 2013. DOI : 10.1038/nmeth.2473.
J. L. Garcia-Cordero; C. Nembrini; A. Stano; J. A. Hubbell; S. J. Maerkl : A high-throughput nanoimmunoassay chip applied to large-scale vaccine adjuvant screening; Integrative Biology. 2013. DOI : 10.1039/c3ib20263a.
J. L. Garcia-Cordero; S. J. Maerkl : Multiplexed surface micropatterning of proteins with a pressure-modulated microfluidic button-membrane; Chemical Communications. 2013. DOI : 10.1039/c2cc37740c.
N. Dénervaud / S. Maerkl; F. Naef (Dir.) : A microfluidic live-cell imaging platform to study large collections of microbial genotypes. Lausanne, EPFL, 2013. DOI : 10.5075/epfl-thesis-5593.
2012
C. Schröter; S. Ares; L. G. Morelli; A. Isakova; K. Hens et al. : Topology and dynamics of the zebrafish segmentation clock core circuit; PLoS Biology. 2012. DOI : 10.1371/journal.pbio.1001364.
R. K. Shultzaberger; S. J. Maerkl; J. F. Kirsch; M. B. Eisen : Probing the Informational and Regulatory Plasticity of a Transcription Factor DNA–Binding Domain; PLoS Genetics. 2012. DOI : 10.1371/journal.pgen.1002614.
H. Niederholtmeyer; L. Xu; S. J. Maerkl : Real-Time mRNA Measurement during an; ACS Synthetic Biology. 2012. DOI : 10.1021/sb300104f.
M. Geertz; D. Shore; S. J. Maerkl : Massively parallel measurements of molecular interaction kinetics on a microfluidic platform; Proceedings Of The National Academy Of Sciences Of The United States Of America. 2012. DOI : 10.1073/pnas.1206011109.
A. S. Rajkumar; S. J. Maerkl : Rapid Synthesis of Defined Eukaryotic Promoter Libraries; Acs Synthetic Biology. 2012. DOI : 10.1021/sb300045j.
R. K. Shultzaberger; S. J. Maerkl; J. F. Kirsch; M. B. Eisen : Probing the Informational and Regulatory Plasticity of a Transcription Factor DNA-Binding Domain; Plos Genetics. 2012. DOI : 10.1371/journal.pgen.1002614.
2011
S. Rockel; M. Geertz; S. J. Maerkl : MITOMI: A Microfluidic Platform for In Vitro Characterization of Transcription Factor–DNA Interaction; Gene Regulatory Networks. 2011. DOI : 10.1007/978-1-61779-292-2_6.
M. Geertz; S. Rockel; S. J. Maerkl : A High-Throughput Microfluidic Method for Generating and Characterizing Transcription Factor Mutant Libraries; Synthetic Gene Networks. 2011. DOI : 10.1007/978-1-61779-412-4_6.
B. Z. He; A. K. Holloway; S. J. Maerkl; M. Kreitman : Does Positive Selection Drive Transcription Factor Binding Site Turnover? A Test with Drosophila Cis-Regulatory Modules; PLoS Genetics. 2011. DOI : 10.1371/journal.pgen.1002053.
F. Rabanera; L. Miguel; S. J. Maerkl : A software-programmable microfluidic device for automated biology; Lab on a Chip. 2011. DOI : 10.1039/C0LC00537A.
2010
L. M. Fidalgo; S. Maerkl : Software-reconfigurable universally-applicable microfluidic platform. 2010. p. -.
S. J. Maerkl : Next generation microfluidic platforms for high-throughput protein biochemistry; Current Opinion in Biotechnology. 2010. DOI : 10.1016/j.copbio.2010.08.010.
M. Geertz; S. J. Maerkl : Experimental strategies for studying transcription factor-DNA binding specificities; Briefings in Functional Genomics. 2010. DOI : 10.1093/bfgp/elq023.
2009
L. Huang; O. J. F. Martin; S. J. Maerkl : Integration of plasmonic trapping in a microfluidic environment; Optics Express. 2009. DOI : 10.1364/OE.17.006018.
S. Maerkl : Integration column: Microfluidic high-throughput screening; Integrative Biology. 2009. DOI : 10.1039/b819762h.
2008
2007
2002
T. Thorsen; S. Maerkl; S. Quake : Microfluidic Large-Scale Integration; Science. 2002. DOI : 10.1126/science.1076996.
Enseignement & Phd
Enseignement
- Life Sciences Engineering,
Programmes doctoraux
- Doctoral Program in Biotechnology and Bioengineering
- Doctoral Program in Microsystems and Microelectronics
- Doctoral program in computer and communication sciences
- Doctoral Program in Molecular Life Sciences
Doctorants
Cai Chun-Jie
Cheng Shiyu
Giaveri Simone
Istomin Ivan
Jammes Fabien Louis Claude Robert
Lavickova Barbora
Michielin Grégoire
Swank Zoe Newell
Yip Hon Ming
Blackburn Matthew Christopher ...
Dénervaud Nicolas ...
Elitas Meltem ...
Niederholtmeyer Henrike Marie ...
Nobs Jean-Bernard ...
Petrova Ekaterina Emilova ...
Rajkumar Arun Stephen ...
Rockel Sylvie ...
Tatárová Zuzana ...
Verpoorte Amanda ...
Volpetti Francesca ...
Woodruff Kristina Pan ...
Cheng Shiyu
Giaveri Simone
Istomin Ivan
Jammes Fabien Louis Claude Robert
Lavickova Barbora
Michielin Grégoire
Swank Zoe Newell
Yip Hon Ming
A dirigé les thèses de
Becker Johannes ...Blackburn Matthew Christopher ...
Dénervaud Nicolas ...
Elitas Meltem ...
Niederholtmeyer Henrike Marie ...
Nobs Jean-Bernard ...
Petrova Ekaterina Emilova ...
Rajkumar Arun Stephen ...
Rockel Sylvie ...
Tatárová Zuzana ...
Verpoorte Amanda ...
Volpetti Francesca ...
Woodruff Kristina Pan ...
Cours
Scientific project design in synthetic biology (iGEM)
Une équipe interdisciplinaire d'étudiants de l'EPFL va concevoir et construire des circuits génétiques avec de nouvelles fonctionnalités. Leur créativité et l'esprit critique sont fortement encouragés.Ils acquièrent des compétences pratiques au laboratoir... 