Cellular responses to external signals begin at the plasma membrane, where the dynamic assembly of receptors or ion channels can regulate cellular activity. Membrane-enveloped viruses, including the human immunodeficiency virus (HIV) also assemble at the plasma membrane, exploiting mechanisms evolved for cellular trafficking. Despite the importance of these processes, our physical paradigm for how proteins form mesoscale assemblies is far from complete.
This is in part a consequence of technical limitations. While the organization and dynamics of membrane proteins are heterogeneous, commonly used fluorescence-based measurements lack information at the molecular scale. In contrast, single molecule measurements limited to looking at only a few molecules in a given cell lack ensemble information. Thus, the study of protein assembly has been limited by a lack of spatially resolved, dynamic information on ensembles of molecules. To overcome these obstacles, we use super-resolution fluorescence imaging techniques combined with live cell imaging and single molecule tracking to determine how the dynamics of protein assembly are coordinated.
From 2016 Associate professor, EPFL, Lausanne, Switzerland
2009-2016 Tenure-track assistant professor, EPFL, Lausanne, Switzerland
2006-2009 Post-Doctoral fellow, National Institutes of Health, Bethesda, MD, USA
2004-2006 Post-Doctoral fellow, Massachusetts Institute of Technology, Cambridge, MA, USA
1999-2004 PhD (Physics) Awarded 06/2004, Harvard University, Cambridge, MA, USA
1993-1997 Bachelors (Cum Laude) Physics & Mathematics, Rice University, Houston, TX, USA
Manoël Prouteau, Ambroise Desfosses, Christian Sieben, Clélia Bourgoint, Nour Lydia Mozaffari, Davide Demurtas, Alok K. Mitra, Paul Guichard, Suliana Manley and Robbie Loewith , “TORC1 Organised in Inhibited Domains (TOROIDs) regulate TORC1 activity,” Nature doi:10.1038/nature24021 (2017)
A Vancevska*, KM Douglass*, V Pfeiffer, S Manley and J Lingner , “The telomeric DNA damage response occurs in the absence of chromatin decompaction,” Genes Dev, 31(6):567-577 (2017)
T Verdier, J Gunzenhäuser, S Manley and M Castelnovo , "Single Particle Maximum Likelihood Reconstruction from Superresolution Microscopy Images," PLoS One, 12(3), e0172943 (2017)
M Bergé, S Campagne, J Mignolet, SJ Holden, L Théraulaz, S Manley, FHT Allain and PH Viollier , "Modularity and determinants of a (bi-)polarization control system from free-living and obligate intracellular bacteria," eLife 5:e20640 (2016)
J Mignolet, SJ Holden, M Bergé, G Panis, L Théraulaz, S Manley and PH Viollier , "Functional dichotomy and distinct nanoscale assemblies of a cell cycle-controlled bipolar zinc-finger regulator," eLife 5:e18647 (2016)
C Deluz, ET Friman, D Strebinger, A Benke, M Raccaud, A Callegari, S Manley and DM Suter , "A role for mitotic bookmarking of SOX2 in pluripotency and differentiation," Genes Dev 30(22) 2538-2550 (2016)
K Douglass , C Sieben, A Archetti, A Lambert, S Manley , "Super-resolution imaging of multiple cells by optimized flat-field epi-illumination," Nat Photonics 10.1038 (2016) Featured in Nat Methods Research Highlights
Y Nasu, A Benke, S Arakawa, GJ Yoshida, G Kawamura, S Manley, S Shimizu, T Ozawa , "In Situ Characterization of Bak Clusters Responsible for Cell Death Using Single Molecule Localization Microscopy," Sci Rep 6:27505 (2016)
L Carlini*, S Holden*, K Douglass* and S Manley , "Correction of a depth-dependent lateral distortion in 3D super-resolution imaging," PLoS One 10(11) e0142949 (2015)
PJ Fabre, A Benke, E Joye, THN Huynh, S Manley and D Duboule , "Nanoscale spatial organization of the HoxD cluster in distinct transcriptional states," Proc Natl Acad Sci USA 112(45) 13964-13969 (2015)
D Sage, H Kirschner, T Pengo, N Stuurman, J Min, S Manley and M Unser , "Quantitative evaluation of software packages for single-molecule localization microscopy." Nat Methods,12(8) 717-724 (2015)
T Pengo, S Holden and S Manley , "PALMsiever: a tool to turn raw data into results for single-molecule localization microscopy," Bioinformatics, 31(5):797-798 (2015)
J Gunzenhäuser, R Wyss and S Manley , "A quantitative approach to evaluate the impact of fluorescent labeling on HIV-Gag assembly by titration of unlabeled proteins," PLoS One, 9(12) e115095 (2014)
J Min*, S Holden*, L Carlini, M Unser, S Manley and JC Ye , "3D high-density localization microscopy using hybrid astigmatic/ biplane imaging and sparse image reconstruction," Biomed Opt Express, 5(11):3935-3948 (2014)
J Min, C Vonesch*, H Kirschner*, L Carlini*, N Olivier, S Holden, S Manley, JC Ye and M Unser , "FALCON: Fast and unbiased reconstruction of high-density super-resolution microscopy data," Sci Rep 4:4577 (2014)
SJ Holden, T Pengo, K Meibom, C Fernandez-Fernandez, J Collier and S Manley , "In vivo quantitative analysis of Caulobacter crescentus z-ring organization by high throughput 3D PALM," Proc Natl Acad Sci USA 111(12):4566-4571 (2014) Featured in Nat Methods Research Highlights
L Carlini, A Benke, L Reymond, G Lukinavicius and S Manley , "Reduced dyes enhance single molecule localization density for live superresolution imaging," ChemPhysChem 15(4):750-755 (2014)
D Keller, M Orpinell, N Olivier, M Wachsmuth, R Mahen, R Wyss, V Hachet, J Ellenberg, S Manley and P Gönczy , "Mechanisms of HsSAS-6 assembly promoting centriole formation in human cells," J Cell Biol 204(5):697-712 (2014)
L Carlini and S Manley , "Live intracellular super-resolution imaging using site-specific stains," ACS Chem Biol 8(12):2643-2648 (2013)
N Olivier , D Keller, P Gönczy and S Manley , "Resolution doubling in 3D STORM imaging through improved buffers," PLoS One 8(7):e69004 (2013)
N Olivier , D Keller, VS Rajan, P Gönczy and S Manley , "Simple buffers for 3D STORM microscopy," Biomed Opt Express 4(6):885-899 (2013)
J Min, C Vonesch, N Olivier, H Kirshner, S Manley, JC Ye and M Unser , "Continuous localization using sparsity constraints for high-density super-resolution microscopy," ISBI (2013)
G Lukinavicius, K Umezawa, N Olivier, A Honigmann, G Yang, T Plass, V Mueller, L Reymond, IR Correa Jr., Z-G Luo, C Schultz, EA Lemke, P Heppenstall, C Eggeling, S Manley and K Johnsson , "A near-infrared fluorophore for live-cell superresolution microscopy of cellular proteins," Nat Chem 5(2):132-139 (2013)
N Hoze, D Nair, E Hosy, C Sieben, S Manley, A Herrmann, J-B Sibarita, D Choquet and D Holcman , “Heterogeneity of AMPA receptor trafficking and molecular interactions revealed by superresolution analysis of live cell imaging,” Proc Natl Acad Sci USA, 109(42):17052-17057 (2012)
J Gunzenhäuser, N Olivier, T Pengo and S Manley , “Quantitative super-resolution imaging reveals protein stoichiometry and nanoscale morphology of assembling HIV-Gag virions,” Nano Lett 12(9):4705-10 (2012)
H Kirschner, T Pengo, N Olivier, D Sage, S Manley, and M Unser “A PSF-based approach to Biplane calibration in 3D super-resolution microscopy,” ISBI (2012) pdf
A Benke*, N Olivier*, J Gunzenhäuser and S Manley , “Multicolor single molecule tracking of stochastically active synthetic dyes,” Nano Lett 12(5):2619-2624 (2012)
A Benke and S Manley , “Live-cell dSTORM of cellular DNA based on direct DNA labeling,” ChemBioChem, 13(2):298-301 (2012) “Very Important Paper” and cover, Featured in Nat Methods Research Highlights
S Banala, D Maurel, S Manley and K Johnsson “A caged, localizable rhodamine derivative for superresolution microscopy,” ACS Chem Biol, 7(2) (2012)
PhD and postdoctoral positions available. Email for more information.
Teaching & PhD
- Doctoral Program in Physics
- Doctoral Program in Molecular Life Sciences
- Doctoral Program in Biotechnology and Bioengineering
- Doctoral Program in Chemistry and Chemical Engineering
- Doctoral Program in Photonics
- Doctoral Program in Computational and Quantitative Biology
The students will acquire knowledge on the fundamental aspects of super-resolutions microscopy. Practical skills include preparation of samples of cells (either bacteria or eukarytic cell culture) for imaging, image acquisition, and data analysis.
The goal of General Physics is to give the student the basic notions to have a better understanding of physical phenomena. This objective is attained when the student can quantitatively analyse the consequences of these effects with the appropriate theore...
In this course we will study the cell (minimum unit of life) and its components. We will study several key cellular features: Membranes, genomes, channels and receptors. We will apply the laws of physics to develop models to make quantitative and predicti...