Thierry Laroche

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Collaborateur technique

thierry.laroche@epfl.ch +41 21 693 96 29 http://biop.epfl.ch/

Nationalité : Suisse

Date de naissance : 12.05.1960

EPFL SV PTECH PTBIOP
AI 0140 (Bâtiment AI)
Station 15
CH-1015 Lausanne

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Données administratives

Compétences

Microscope Electronique
  • Philips 400
  • CM10
  • Microtome
  • Ultramicrotome
  • Embedding technique
  • Shadowing technique
  • Confocal Microscopie et Multiphoton
  • lsm410-510-Meta-700-710
  • Sp2-Sp5wll-Sp8wll-Sp5 2P
  • STED
  • STORM
  • SIM
  • Light Sheet Microscope

  • Spining Disk
  • Time Lapse Acquisition (Living Cell)
  • Till 2-4-5
  • WF Microscopie
  • Axiovert-Axioscope-Z1
  • DMI4000-DM5500
  • Ax80-AX70
  • Delta Vision
  • -Expertise
  • 2D Time
  • 3D
  • 4D
  • Spectral
  • Colocalisation
  • FRAP
  • FRET
  • Traking
  • Software
  • MetaMorph
  • IJ
  • Imaris
  • Huygens
  • Photosphop
  • -Image Processing
  • Deconvolution
  • Colocalisation
  • Publications

    Autres publications

    Gasser, S.M., Laroche, T., Falquet, J., Boy de la Tour, E. and Laemmli, U.K (1986)
    J. Mol. Biol., 155, 613 - 629.
    Metaphase Chromosome Structure: Involvement of Topoisomerase II
    Hofmann, J.F-X., Laroche, T., Brand, A.H. and Gasser, S.M. (1989)
    Cell, 57, 725 - 737.
    RAP-1 is necessary for DNA loop formation in vitro at the silent mating type locus, HML.
    Karwan, R.M., Laroche, T., Wintersberger, U., Gasser, S.M. and Binder, M. (1990)
    J. Cell Sci. 96, 451 - 458.
    Ribonuclease H(70) Is a Component of the Yeast Nuclear Scaffold
    Amati, B., Pick, L., Laroche, T. and Gasser, S.M. (1990)
    EMBO J., 9, 4007 - 4017.
    Nuclear Scaffold Attachment Stimulates but is not Necessary for ARS Activity in S. cerevisiae: Analysis of the Drosophila ftz SAR
    Cardenas, M.E., Laroche, T. and Gasser, S.M. (1990)
    J. Cell Sci., 96, 439 -450.
    The Composition and Morphology of Yeast Nuclear Scaffolds J. Cell Sci., 96, 439 -450.
    Klein, F., Laroche, T., Cardenas, M.E., Hofmann, J.F.X., Schweizer, D. and Gasser, S.M. (1992)
    J. Cell Biol., 117, 935 - 948.
    Localization of RAP1 and Topoisomerase II in Nuclei and Meiotic Chromosomes of Yeast
    Palladino, F., Laroche, T., Gilson, E., Pillus, L. and Gasser, S.M. (1993)
    CSH Symp. Quant. Biol. 58, Cold Spring Harbor Press, New York, 733 - 746
    The positioning of yeast telomeres depends on SIR3, SIR4, and the integrity of the nuclear membrane.
    Gilson E, Laroche T, Gasser SM.(1993)
    Trends Cell Biol.Apr;3(4):128-34.
    Telomeres and the functional architecture of the nucleus.
    Palladino, F., Laroche, T., Gilson, E., Axelrod, A., Pillus, L. and Gasser, S.M. (1993)
    Cell, 75, 543 - 555.
    SIR3 and SIR4 proteins are required for the positioning and integrity of yeast telomeres
    Gilson, E., M�ller, T., Sogo, J., Laroche, T. and Gasser, S.M. (1994)
    Nucl. Acids Res., 22, 5310 - 5320.9.
    RAP1 promotes single- to double-strand association of yeast telomeric DNA: implications for telomere-telomere interactions
    Grunstein, M., Hecht, A., Fisher-Adams, G., Wan, J., Mann, R.K., Strahl-Bolsinger, S., Laroche, T. and Gasser, S.M. (1995)
    J. Cell Sci., 19, 29 - 36.
    The regulation of euchromatin and heterochromatin by histones in yeast.
    Hecht, A., Laroche, T., Strahl-Bolsinger, S., Gasser, S.M. and Grunstein, M. (1995)
    Cell, 80, 583 - 592.
    Histone H3 and H4 N-termini interact with SIR3 and SIR4 proteins: a molecular model for the formation heterochromatin in yeast
    11. Cockell, M., Palladino, F., Laroche, T., Kyrion, G., Liu, C., Lustig, A.J. and Gasser, S.M. (1995)
    J. Cell Biol., 129, 909 - 924.
    The carboxy-termini of SIR4 and RAP1 affect SIR3 localization: Evidence for a multi-component complex required for yeast telomeric silencing
    Gotta, M., Laroche, T., Formenton, A., Maillet, L., Scherthan, H. and Gasser, S.M. (1996)
    J. Cell Biol., 134, 1349 - 1363.
    The clustering of telomeres and colocalization with Rap1, Sir3 and Sir4 proteins in wild-type S. cerevisiae
    Kennedy, B.K., Gotta, M., Sinclair, D.A., Mills, K., McNabb, D.S., Murthy, M., Pak, S.M., Laroche, T., Gasser, S.M. and Guarente, L. (1997)
    Cell, 89, 381 - 391
    Redistribution of silencing proteins from telomeres to the nucleolus is associated with extension of life span in S. cerevisiae.
    Gotta, M., Strahl-Bolsinger, S., Renauld, H., Kennedy, B.K., Laroche, T., Grunstein, M. and Gasser, S.M. (1997)
    EMBO J., 16, 3243 - 3255.
    Localization of Sir2p: the nucleolus as a compartment for Silent Information Regulators
    Bilaud T., Brun, C., Ancelin, K., Koering C.E., and Laroche, T. (1997)
    Nature Genet., 17, 236 - 239
    Telomeric localization of TRF2, a novel human telobox protein.
    Laroche, T., Martin, S.G., Gotta, M., Gorham, H.C., Pryde, F.E., Louis, E.J. and Gasser, S.M. (1998)
    Curr. Biology 8, 653 - 656
    Mutation of yeast Ku genes disrupts the subnuclear organization of telomeres.
    Gasser SM, Gotta M, Renauld H, Laroche T, Cockell M.
    Novartis Found Symp. 1998;214:114-26; discussion 126-32.
    Nuclear organization and silencing: trafficking of Sir proteins.
    Martin, S.G., Laroche, T., Suka, N., Grunstein, M. and Gasser, S.M. (1999)
    Cell, 97, 621 - 633
    Relocalization of telomeric Ku and SIR proteins in response to DNA strand breaks in yeast.
    Gotta M, Laroche T, Gasser SM.(1999)
    Methods Enzymol.304:663-72.
    Analysis of nuclear organization in Saccharomyces cerevisiae.
    Pollice A, Zibella MP, Bilaud T, Laroche T, Pulitzer JF, Gilson E. (2000)
    Biochem. Biophys. Res. Commun. 268: 909 - 915
    In vitro binding of nucleolin to double-stranded telomeric DNA.
    Leupin O, Zaru R, Laroche T, Muller S, Valitutti S.(2000)
    Curr. Biology 10 : 277-280
    Exclusion of CD45 from the T-cell receptor signaling area in antigen-stimulated T lymphocytes.
    Laroche, T., Martin, S.G., Tsai-Pflugfelder, M. and Gasser, S.M. (2000)
    J. Struct. Biol., 129: 159 - 174.
    The Dynamics of Yeast Telomeres and Silencing Proteins through the Cell Cycle.
    Koering CE, Fourel G, Binet-Brasselet E, Laroche T, Klein F, Gilson E. (2000)
    Nucleic Acids Res. 13: 2519-2526.
    Identification of high affinity Tbf1p-binding sites within the budding yeast genome.
    Stone, E.M., Heun, P., Laroche, T., Pillus, L. and Gasser, S.M. (2000)
    Curr. Biology, 10: 373 � 382.
    MAP kinase signaling induces nuclear reorganization in budding yeast.
    Perrod, S., Cockell, M.M., Laroche, T., Renauld, H., Bonnard, C. and Gasser, S.M. (2001)
    EMBO J. 20(1-2):197-209
    A cytosolic homologue of Sir2p modulates rDNA and telomeric silencing in yeast,
    Heun, P., Laroche, T., Raghuraman, M. K. and Gasser, S.M. (2001)
    J. Cell Biol., 152, 385 - 400.
    The positioning and dynamics of origins of replication in the budding yeast nucleus
    Heun, P., Laroche, T., Shimada, K., Furrer, P. and Gasser, S.M. (2001)
    Science, 7; 294 (5549): 2181-6
    Chromosome dynamics in the yeast interphase nucleus
    Gartenberg MR, Neumann FR, Laroche T, Blaszczyk M, Gasser SM.
    Cell. 2004 Dec 29;119(7):955-67.
    Sir-mediated repression can occur independently of chromosomal and subnuclear contexts.
    Nagoshi E, Saini C, Bauer C, Laroche T, Naef F, Schibler U.
    Cell. 2004 Nov 24;119(5):693-705
    Circadian gene expression in individual fibroblasts: cell-autonomous and self-sustained oscillators pass time to daughter cells.
    Bystricky K, Laroche T, van Houwe G, Blaszczyk M, Gasser SM.
    J Cell Biol. 2005 Jan 31;168(3):375-87.
    Chromosome looping in yeast: telomere pairing and coordinated movement reflect anchoring efficiency and territorial organization.
    Kobel S, Limacher M, Gobaa S, Laroche T, Lutolf MP.
    Langmuir. 2009 Aug 4;25(15):8774-9
    Micropatterning of hydrogels by soft embossing
    Varela E, Shimada K, Laroche T, Leroy D, Gasser SM.
    EMBO J. 2009 Jun 3;28(11):1562-75. Epub 2009 Apr 23
    Lte1, Cdc14 and MEN-controlled Cdk inactivation in yeast coordinate rDNA decompaction with late telophase progression.
    Allaman I, Gavillet M, B�langer M, Laroche T, Viertl D, Lashuel HA, Magistretti PJ.
    J Neurosci. 2010 Mar 3;30(9):3326-38.
    Amyloid-beta aggregates cause alterations of astrocytic metabolic phenotype: impact on neuronal viability.
    Maurel D, Banala S, Laroche T, Johnsson K.
    ACS Chem Biol. 2010 May 21;5(5):507-16.
    Photoactivatable and photoconvertible fluorescent probes for protein labeling.
    B�langer M, Yang J, Petit JM, Laroche T, Magistretti PJ, Allaman I.
    J Neurosci. 2011 Dec 14;31(50):18338-52.
    Role of the glyoxalase system in astrocyte-mediated neuroprotection.