Curriculum Vitae CV
 

Many biomolecules show remarkably more complex behavior at the single-molecule level than it is observed in bulk, ensemble-averaged experiments. It is now evident that sparsely populated molecular sub-states are vital for the biological function. Insights into complex behavior can be gained through manipulation, imaging or sensing of single biomolecules. 
 Single-molecule techniques can provide us with extraordinarily clear and often surprising views of biomolecules in action. Development of appropriate instrumentation has been identified as the key ingredient for advances in biophysical sciences. Three most important examples here are single molecule localization microscopy (SMLM), optical tweezers and nanopores. SMLM made it possible to “see” by visualizing single fluorophores in living cells while laser-based optical tweezers allowed us to “touch” with experiments on physical forces involved in stretching and binding of biomolecules, while nanopores, the simplest and the most recent single molecule technique allows for rapid and high-throughput biosensing and discrimination of atto–molar molecule concentrations. 

From October 2015 Associate Professor
 2008-2015 Tenure-Track Assistant Professor
 2004-2007 Postdoc at the University of California, Berkeley in the group of Prof.Liphardt
 2003 PhD student of Prof. Dietler in Laboratory of Physics of Living Matter, University of Lausanne 
 1999 Diploma thesis on the subject of the Raman spectroscopy of beta carotene
 1994-1999 Physics department at the University of Zagreb
 1994 baccalaureate, Classical gymnasium 

My lab works in the research field that can be termed single molecule biophysics. We develop techniques and methodologies based on optical imaging, biosensing and single molecule manipulation with the aim to monitor the behavior of individual biological molecules and complexes in vitro and in live cells. Our current research is focused on three major directions:
 
 (i) Developing and using nanopores as platform for molecular sensing and manipulation. In particular we focus on solid-state nanopores realized either in glass nanocapillaries, or on suspended 2d-material membranes and standard silicon-nitride membranes. 
 
 (ii) Studying how biomolecules function, especially how proteins and nucleic acids interact, using force-based manipulation single-molecule techniques, in particular optical tweezers, optical wrench system, Anti- Brownian Electrokinetic (ABEL) trap and combination of nanopore/nanocapillaries with OT. 
 
 (iii) Developing super-resolution optical microscopy, based on single molecule localizations (SMLM) in cells with molecular-scale resolution, with an aim to extract quantitative information. 

2016 CCMX Materials challenge award 
2015 SNSF-ERC Consolidator Grant
2010 ERC Starting Grant
2003 Fellowship of the Swiss National Science Foundation

Patent Number: WO07079411
 ALIGNMENT, TRANSPORTATION AND INTEGRATION OF NANOWIRES USING OPTICAL TRAPPING
 Publication date: 2007-07-12.
 Link

Patent Number:PCT/IB2013/052093
MANUFACTURING OF ORIFICES IN GLASS LIKE MATERIALS, E.G. NANOCAPILLARIES AND OBJECT OBTAINED ACCORDING TO THIS PROCESS
Publication date: 2014-25-03
Link US patent

Patent Number: WO2015121394
MOLECULAR SENSING DEVICE-
Publication date: 2015-20-08
Link

Patent Number: WO2016142925A1
NANOPORE FORMING METHOD AND USES THEREOF WO2016142925A1
Publication date :2016-15-09 
Link

Patent Number: WO2018002099 

OSMOTIC POWER GENERATION IN 2D NANOPORES
Publication date :2018-15-09 
Publication date :2016-15-09  

Patent application:

BENCHMARKING OF SINGLE IMAGING DATASETS

19 180 900.3 - Your ref.: 6.1943 - Our ref.: 34623EP

 

Patent application

SYSTEMS AND METHODS FOR DIGITAL INFORMATION DECODING AND DATA STORAGE IN HYBRID MACROMOLECULES
2020 September