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Many biomolecules show remarkably more complex behavior at the single-molecule level than it is observed in bulk, ensemble-averaged experiments. It is now evident that sparsely populated molecular sub-states are vital for the biological function. Insights into complex behavior can be gained through manipulation, imaging or sensing of single biomolecules.
Single-molecule techniques can provide us with extraordinarily clear and often surprising views of biomolecules in action. Development of appropriate instrumentation has been identified as the key ingredient for advances in biophysical sciences. Three most important examples here are single molecule localization microscopy (SMLM), optical tweezers and nanopores. SMLM made it possible to see by visualizing single fluorophores in living cells while laser-based optical tweezers allowed us to touch with experiments on physical forces involved in stretching and binding of biomolecules, while nanopores, the simplest and the most recent single molecule technique allows for rapid and high-throughput biosensing and discrimination of attomolar molecule concentrations.
My lab works in the research field that can be termed single molecule biophysics. We develop techniques and methodologies based on optical imaging, biosensing and single molecule manipulation with the aim to monitor the behavior of individual biological molecules and complexes in vitro and in live cells. Our current research is focused on three major directions:
(i) Developing and using nanopores as platform for molecular sensing and manipulation. In particular we focus on solid-state nanopores realized either in glass nanocapillaries, or on suspended 2d-material membranes and standard silicon-nitride membranes.
(ii) Studying how biomolecules function, especially how proteins and nucleic acids interact, using force-based manipulation single-molecule techniques, in particular optical tweezers, optical wrench system, Anti- Brownian Electrokinetic (ABEL) trap and combination of nanopore/nanocapillaries with OT.
(iii) Developing super-resolution optical microscopy, based on single molecule localizations (SMLM) in cells with molecular-scale resolution, with an aim to extract quantitative information.
From October 2015 Associate Professor
2008-2015 Tenure-Track Assistant Professor
2004-2007 Postdoc at the University of California, Berkeley in the group of Prof.Liphardt
2003 PhD student of Prof. Dietler in Laboratory of Physics of Living Matter, University of Lausanne
1999 Diploma thesis on the subject of the Raman spectroscopy of beta carotene
1994-1999 Physics department at the University of Zagreb
1994 baccalaureate, Classical gymnasium
2015 SNSF-ERC Consolidator Grant
2010 ERC Starting Grant
2003 Fellowship of the Swiss National Science Foundation
Patent Number: WO07079411
ALIGNMENT, TRANSPORTATION AND INTEGRATION OF NANOWIRES USING OPTICAL TRAPPING
Publication date: 2007-07-12.
MANUFACTURING OF ORIFICES IN GLASS LIKE MATERIALS, E.G. NANOCAPILLARIES AND OBJECT OBTAINED ACCORDING TO THIS PROCESS
Publication date: 2014-25-03
Patent Number: WO2015121394
MOLECULAR SENSING DEVICE-
Publication date: 2015-20-08
NANOPORE FORMING METHOD AND USES THEREOF
PCT / P1899EP00; Publication date: Pending
Fields of expertise
Solid State Nanopores
Second Harmonic Generation
Super -resolution microscopy
Peer reviewed journal articlesOnline data is not available
Teaching & PhD
- Life Sciences Engineering,
- Doctoral Program in Biotechnology and Bioengineering
- Doctoral Program in Physics
- Doctoral Program in Microsystems and Microelectronics
- Doctoral Program in Photonics
- Doctoral Program in Chemistry and Chemical Engineering
- Doctoral Program in Materials Science and Engineering
- Doctoral Program in Computational and Quantitative Biology
PhD StudentsBabashah Hossein
Davis Sebastian James
Descloux Adrien Charles-François Raymond
Macha Michal Daniel
Planchette Arielle Louise
Past PhD StudentsAnnibale Paolo ...
Bulushev Roman ...
Dutto Fabrizia ...
Feng Jiandong ...
Graf Michael ...
Kayci Metin ...
Raillon Camille Alice ...
Shivanandan Arun ...
This module serves as an introduction to the area of biophotonics. The approach is multidisciplinary .The course is mainly knowledge-based but students will benefit from the skills learned by carrying out problem solving and by completing the assignment....
Advanced Bioengineering Methods Laboratories (ABML) offers laboratory practice and data analysis. These active sessions present a variety of techniques employed in the bioengineering field and matching a quantitative and technological based approach.