Fields of expertise
Solid State Nanopores
Second Harmonic Generation
Super -resolution microscopy
Single-molecule techniques can provide us with extraordinarily clear and often surprising views of biomolecules in action. Development of appropriate instrumentation has been identified as the key ingredient for advances in biophysical sciences. Three most important examples here are single molecule localization microscopy (SMLM), optical tweezers and nanopores. SMLM made it possible to see by visualizing single fluorophores in living cells while laser-based optical tweezers allowed us to touch with experiments on physical forces involved in stretching and binding of biomolecules, while nanopores, the simplest and the most recent single molecule technique allows for rapid and high-throughput biosensing and discrimination of attomolar molecule concentrations.
2008-2015 Tenure-Track Assistant Professor
2004-2007 Postdoc at the University of California, Berkeley in the group of Prof.Liphardt
2003 PhD student of Prof. Dietler in Laboratory of Physics of Living Matter, University of Lausanne
1999 Diploma thesis on the subject of the Raman spectroscopy of beta carotene
1994-1999 Physics department at the University of Zagreb
1994 baccalaureate, Classical gymnasium
(i) Developing and using nanopores as platform for molecular sensing and manipulation. In particular we focus on solid-state nanopores realized either in glass nanocapillaries, or on suspended 2d-material membranes and standard silicon-nitride membranes.
(ii) Studying how biomolecules function, especially how proteins and nucleic acids interact, using force-based manipulation single-molecule techniques, in particular optical tweezers, optical wrench system, Anti- Brownian Electrokinetic (ABEL) trap and combination of nanopore/nanocapillaries with OT.
(iii) Developing super-resolution optical microscopy, based on single molecule localizations (SMLM) in cells with molecular-scale resolution, with an aim to extract quantitative information.
2015 SNSF-ERC Consolidator Grant
2010 ERC Starting Grant
2003 Fellowship of the Swiss National Science Foundation
ALIGNMENT, TRANSPORTATION AND INTEGRATION OF NANOWIRES USING OPTICAL TRAPPING
Publication date: 2007-07-12.
MANUFACTURING OF ORIFICES IN GLASS LIKE MATERIALS, E.G. NANOCAPILLARIES AND OBJECT OBTAINED ACCORDING TO THIS PROCESS
Publication date: 2014-25-03
Link US patent
Patent Number: WO2015121394
MOLECULAR SENSING DEVICE-
Publication date: 2015-20-08
Patent Number: WO2016142925A1
NANOPORE FORMING METHOD AND USES THEREOF WO2016142925A1
Publication date :2016-15-09
Publication date :2018-15-09
Publication date :2016-15-09
ERC Starting Grant
SNSF-ERC Consolidator Grant
 Fundamental Applications of Nanopores: Controlled DNA Translocations to NanofluidicsLausanne, EPFL, 2020. DOI : 10.5075/epfl-thesis-7693.
Peer reviewed journal articles
 Wetting of nanopores probed with pressurePhysical Chemistry Chemical Physics. 2021-02-28. DOI : 10.1039/d1cp00253h.
 Recent Advances and Prospects in the Research of Nascent AdhesionsFrontiers In Physiology. 2020-12-04. DOI : 10.3389/fphys.2020.574371.
 Towards artificial mechanosensingNature Materials. 2020-10-01. DOI : 10.1038/s41563-020-00811-5.
 Self-Blinking Dyes Unlock High-Order and Multiplane Super-Resolution Optical Fluctuation ImagingAcs Nano. 2020-07-28. DOI : 10.1021/acsnano.0c04602.
 Waveguide-Based Platform for Large-FOV Imaging of Optically Active Defects in 2D MaterialsAcs Photonics. 2019-12-01. DOI : 10.1021/acsphotonics.9b01103.
 Spatiotemporal Imaging of Water in Operating Voltage-Gated Ion Channels Reveals the Slow Motion of Interfacial IonsNano Letters. 2019-10-03. DOI : 10.1021/acs.nanolett.9b02024.
 Wafer-scale MOCVD growth of monolayer MoS2 on sapphire and SiO2Nano Research. 2019-10-01. DOI : 10.1007/s12274-019-2502-9.
 Facile Production of Hexagonal Boron Nitride Nanoparticles by Cryogenic ExfoliationNano Letters. 2019-08-01. DOI : 10.1021/acs.nanolett.9b01913.
 Transverse Detection of DNA in a MoS2 NanoporeBiophysical Journal. 2018. DOI : 10.1016/j.bpj.2017.11.1005.
 Fluorescent Nanodiamonds in Biological and Biomedical Imaging and SensingSuper-Resolution Imaging in Biomedicine; Taylor & Francis Group, 6000 Broken Sound Parkway NW, Suite 300, Boca Raton, FL 33487-2742: CRC Press, 2016.
 Molybdenum Disulfide Nanopores: Why 3 Atoms are Better than One?2015. 59th Annual Meeting of the Biophysical-Society, Baltimore, MD, FEB 07-11, 2015. p. 489A-489A.
 Investigating Cellular Focal Adhesions on Nano-Patterned Substrates with Dual Color Photo-Activated Localization Microscopy2015. 59th Annual Meeting of the Biophysical-Society, Baltimore, MD, FEB 07-11, 2015. p. 359A-359A.
 Electron Spin Resonance of Nitrogen-Vacancy Defects Embedded in Single Nanodiamonds in an ABEL TrapNano Letters. 2014. DOI : 10.1021/nl5023964.
 Shrinking Nanocapillaries to Low Noise Nanopores for Single Molecule Detection2014. 58th Annual Meeting of the Biophysical-Society, San Francisco, CA, FEB 15-19, 2014. p. 633A-633A.
 Combination of Optical Tweezers with Nanocapillaries as System for Estimation of DNA/Ligand Interactions2014. 58th Annual Meeting of the Biophysical-Society, San Francisco, CA, FEB 15-19, 2014. p. 393A-393A.
 Niobates Nanowires: Synthesis, Characterization and ApplicationsNanowires - Implementations and Applications; In Tech, 2011. p. 509-524.
 Electrophoretic threading kinetics of optically trapped DNA through synthetic nanoporesBiophysical Journal. 2007.
 Study of DNA in "glasslike state" by atomic force microscopy: Importance of substratesJapanese Journal of Applied Physics Part 1-Regular Papers Brief Communications & Review Papers. 2006. DOI : 10.1143/JJAP.45.2345.
 Complex characterization of physiology solution based magnetic fluidIndian Journal of Engineering and Materials Sciences. 2004.
 Study of probes and substrates for low temperature atomic force microscopy and biological applications2003.
 A low-temperature ultrahigh vacuum atomic force microscope for biological applicationsReview of Scientific Instruments. 2003. DOI : 10.1063/1.1532840.
 Low noise current-to-voltage converter and vibration damping system for a low-temperature ultrahigh vacuum scanning tunneling microscopeReview of Scientific Instruments. 2003. DOI : 10.1063/1.1533100.
 Characterization of atomic force microscope probes at low temperaturesJournal of Applied Physics. 2003. DOI : 10.1063/1.1604952.
Teaching & PhD
Life Sciences Engineering