Aleksandra Radenovic

aleksandra.radenovic@epfl.ch +41 21 693 73 71 http://lben.epfl.ch

vCard

Nationality: Croatian and Swiss

EPFL STI IBI-STI LBEN
BM 2140 (Bâtiment BM)
Station 17
1015 Lausanne

+41 21 693 73 71
+41 21 693 11 61
Office: BM 2140
EPFL › STI › IBI-STI › LBEN

Website: https://lben.epfl.ch/

+41 21 693 73 71
EPFL › SV › SV-SSV › SSV-ENS

Website: https://sv.epfl.ch/education

+41 21 693 73 71
EPFL › STI › STI-SMT › SMT-ENS

+41 21 693 73 71
EPFL › STI › IBI-STI › IBI-STI-GE

Website: https://bioengineering.epfl.ch/

+41 21 693 73 71
EPFL › STI › STI-DEC › STI-DIR

+41 21 693 73 71
EPFL › SV › SV-DEC › SV-DIR

+41 21 693 73 71
EPFL › VPA › VPA-AVP-CP › CIME › CIME-CD

Expertise

Single molecule biophysics
Solid State Nanopores
2-D materials
Optical tweezers
Second Harmonic Generation
Super -resolution microscopy
Signaling

Current work

My lab works in the research field that can be termed single molecule biophysics. We develop techniques and methodologies based on optical imaging, biosensing and single molecule manipulation with the aim to monitor the behavior of individual biological molecules and complexes in vitro and in live cells. Our current research is focused on three major directions:
(i) Developing and using nanopores as platform for molecular sensing and manipulation. In particular we focus on solid-state nanopores realized either in glass nanocapillaries, or on suspended 2d-material membranes and standard silicon-nitride membranes.
(ii) Studying how biomolecules function, especially how proteins and nucleic acids interact, using force-based manipulation single-molecule techniques, in particular optical tweezers, optical wrench system, Anti- Brownian Electrokinetic (ABEL) trap and combination of nanopore/nanocapillaries with OT.
(iii) Developing super-resolution optical microscopy, based on single molecule localizations (SMLM) in cells with molecular-scale resolution, with an aim to extract quantitative information.

Mission

Many biomolecules show remarkably more complex behavior at the single-molecule level than it is observed in bulk, ensemble-averaged experiments. It is now evident that sparsely populated molecular sub-states are vital for the biological function. Insights into complex behavior can be gained through manipulation, imaging or sensing of single biomolecules.
Single-molecule techniques can provide us with extraordinarily clear and often surprising views of biomolecules in action. Development of appropriate instrumentation has been identified as the key ingredient for advances in biophysical sciences. Three most important examples here are single molecule localization microscopy (SMLM), optical tweezers and nanopores. SMLM made it possible to «see» by visualizing single fluorophores in living cells while laser-based optical tweezers allowed us to «touch» with experiments on physical forces involved in stretching and binding of biomolecules, while nanopores, the simplest and the most recent single molecule technique allows for rapid and high-throughput biosensing and discrimination of atto–molar molecule concentrations.

Biography Awards Publications Teaching & PhD

From April 2021 Full Professor
2015 -2021 Associate Professor
2008-2015 Tenure-Track Assistant Professor
2004-2007 Postdoc at the University of California, Berkeley in the group of Prof.Liphardt
2003 PhD student of Prof. Dietler in Laboratory of Physics of Living Matter, University of Lausanne
1999 Diploma thesis on the subject of the Raman spectroscopy of beta carotene
1994-1999 Physics department at the University of Zagreb
1994 baccalaureate, Classical gymnasium

Awards/Achievements

2016 CCMX Materials challenge award
2015 SNSF-ERC Consolidator Grant
2010 ERC Starting Grant
2003 Fellowship of the Swiss National Science Foundation

Curriculum Vitae

Curriculum Vitae CV

Patents

Patent Number: WO07079411
ALIGNMENT, TRANSPORTATION AND INTEGRATION OF NANOWIRES USING OPTICAL TRAPPING
Publication date: 2007-07-12.
Link
Patent Number:PCT/IB2013/052093
MANUFACTURING OF ORIFICES IN GLASS LIKE MATERIALS, E.G. NANOCAPILLARIES AND OBJECT OBTAINED ACCORDING TO THIS PROCESS
Publication date: 2014-25-03
Link US patent
Patent Number: WO2015121394
MOLECULAR SENSING DEVICE-
Publication date: 2015-20-08
Link
Patent Number: WO2016142925A1
NANOPORE FORMING METHOD AND USES THEREOF WO2016142925A1
Publication date :2016-15-09
Link
Patent Number: WO2018002099 OSMOTIC POWER GENERATION IN 2D NANOPORES
Publication date :2018-15-09
Publication date :2016-15-09
Link Link US patent
Patent application:BENCHMARKING OF SINGLE IMAGING DATASETS19 180 900.3 - Your ref.: 6.1943 - Our ref.: 34623EP Patent applicationSYSTEMS AND METHODS FOR DIGITAL INFORMATION DECODING AND DATA STORAGE IN HYBRID MACROMOLECULES
2020 September

Awards

SNSF Fellowship

Swiss National Science Foundation

2003

ERC Starting Grant

European Research Council

2010

SNSF-ERC Consolidator Grant

2015

ERC Advanced Grant

2021

CCMX Materials challenge award

2016

Optica Fellow

Optica

2021

LBEN Thesis

[16] 2D MoS2 Nanopores: Wafer-scale Fabrication and Monolayer Stability for Long-term Single-Molecule Sensing

M. Thakur / A. Radenovic (Dir.)

Lausanne, EPFL, 2022. DOI : 10.5075/epfl-thesis-9538.

Aleksandra Radenovic

Expertise

Current work

Mission

Awards/Achievements

Curriculum Vitae

Patents

Awards

LBEN Thesis

[16] 2D MoS2 Nanopores: Wafer-scale Fabrication and Monolayer Stability for Long-term Single-Molecule Sensing

[15] Molybdenum disulphide nanoporous membranes as nanofluidic platforms - large-area engineering and study

[14] Multidisciplinary Investigation of the Gut-Brain Ecosystem in a Model of Alzheimer's Disease

[13] Exploring optically active defects in wide-bandgap materials using fluorescence microscopy

[12] Biophysical applications of correlative scanning probe and super-resolution microscopy

[11] Fundamental Applications of Nanopores: Controlled DNA Translocations to Nanofluidics

[10] Electrochemical and morphological engineering of 2D materials for nanopore sensing

[9] Development of novel experimental and computational methods for three-dimensional coherent and super-resolution microscopy

[8] 2D nanopores: fabrication, energy harvesting and field-effect sensing

[7] Nanocapillaries combined with optical tweezers as a single molecule technique for studying DNA-protein complexes

[6] Probing chemical structures and physical processes with nanopores

[5] Nanoscale Magnetometry with Single Fluorescent Nanodiamonds Manipulated in an Anti-Brownian Electrokinetic Trap

[4] Analytical Methods, Correlative Microscopy and Software Tools for Quantitative Single Molecule Localization Microscopy

[3] Alkaline niobate nanostructures as opto-mechanical probes

[2] Nanopore sensing of single molecules application to RNAP-DNA complexes, fabrication of graphene-nanopore devices and translocation algorithm analysis

[1] Investigating the Impact of Single Molecule Fluorescence Dynamics on Photo Activated Localization Microscopy Experiments

Peer reviewed journal articles

2024

[159] Fluorescence microscopy: A statistics-optics perspective

[158] CVD graphene contacts for lateral heterostructure MoS2 field effect transistors

[157] Label-Free Techniques for Probing Biomolecular Condensates

[156] Nanofluidic logic with mechano-ionic memristive switches

[155] Open-source microscope add-on for structured illumination microscopy

[154] AI-driven detection and analysis of label-free protein aggregates

2023

[153] Liquid-activated quantum emission from native hBN defects for nanofluidic sensing

[152] Confinement-Controlled Water Engenders Unusually High Electrochemical Capacitance

[151] Selective Growth of van der Waals Heterostructures Enabled by Electron-Beam Irradiation

[150] Nature-Inspired Stalactite Nanopores for Biosensing and Energy Harvesting

[149] Spatially multiplexed single-molecule translocations through a nanopore at controlled speeds

[148] Nanoscale thermal control of a single living cell enabled by diamond heater-thermometer

[147] The Three-Phase Contact Potential Difference Modulates the Water Surface Charge

[146] The Three-Phase Contact Potential Difference Modulates the Water Surface Charge

[145] Optical imaging of the small intestine immune compartment across scales

[144] High durability and stability of 2D nanofluidic devices for long-term single-molecule sensing

[143] Defect engineering of 2D material for biosensing applications

[142] Imaging of interactions of biomolecules with nanomaterials with interferometric scattering microscopy

[141] Substitutional p‐type Doping in NbS2‐MoS2 Lateral Heterostructures Grown by MOCVD

[140] Synthesis of Fluorescent Cyclic Peptides via Gold(I)-Catalyzed Macrocyclization

[139] A large-scale integrated vector–matrix multiplication processor based on monolayer molybdenum disulfide memories

[138] How to Achieve Large-Area Ultra-Fast Operation of MoS 2 Monolayer Flash Memories?

2022

[137] High-Throughput Nanopore Fabrication and Classification Using Xe-Ion Irradiation and Automated Pore-Edge Analysis

[136] Wafer-scale MoS2 with water-vapor assisted showerhead MOCVD

[135] Stress induced delamination of suspended MoS2 in aqueous environments

[134] Stable Al2O3 Encapsulation of MoS2 ‐FETs Enabled by CVD Grown h‐BN

[133] Three-step, transfer-free growth of MoS2/WS2/graphene vertical van der Waals heterostructure

[132] Engineering Optically Active Defects in Hexagonal Boron Nitride Using Focused Ion Beam and Water

[131] High Performance Semiconducting Nanosheets via a Scalable Powder-Based Electrochemical Exfoliation Technique

[130] Statistical distortion of supervised learning predictions in optical microscopy induced by image compression

[129] Zero-Bias Power-Detector Circuits based on MoS<sub>2</sub> Field-Effect Transistors on Wafer-Scale Flexible Substrates

[128] Low-Power Artificial Neural Network Perceptron Based on Monolayer MoS2

[127] Bacterial nanopores open the future of data storage

[126] Flat-Band-Induced Many-Body Interactions and Exciton Complexes in a Layered Semiconductor

2021

[125] Time-Resolved Scanning Ion Conductance Microscopy for Three-Dimensional Tracking of Nanoscale Cell Surface Dynamics

[124] Rhesus Blood Typing within a Few Seconds by Packing-Enhanced Nanoscattering on Individual Erythrocytes

[123] Superconducting 2D NbS2 Grown Epitaxially by Chemical Vapor Deposition

[122] Anomalous interfacial dynamics of single proton charges in binary aqueous solutions

[121] Bio-orthogonal Red and Far-Red Fluorogenic Probes for Wash-Free Live-Cell and Super-resolution Microscopy

[120] Experimental Combination of Super-Resolution Optical Fluctuation Imaging with Structured Illumination Microscopy for Large Fields-of-View

[119] Correlative 3D microscopy of single cells using super-resolution and scanning ion-conductance microscopy

[118] Direct Growth of Hexagonal Boron Nitride on Photonic Chips for High-Throughput Characterization

[117] Adaptive optics enables multimode 3D super-resolution microscopy via remote focusing

[116] High resolution optical projection tomography platform for multispectral imaging of the mouse gut

[115] Parameter-free rendering of single-molecule localization microscopy data for parameter-free resolution estimation

[114] From Water Solutions to Ionic Liquids with Solid State Nanopores as a Perspective to Study Transport and Translocation Phenomena

[113] Super-resolved Optical Mapping of Reactive Sulfur-Vacancies in Two-Dimensional Transition Metal Dichalcogenides

[112] Wetting of nanopores probed with pressure

[111] Electrochemical Functionalization of Selectively Addressed MoS2 Nanoribbons for Sensor Device Fabrication

[110] Decoding Digital Information Stored in Polymer by Nanopore