Didier Trono
EPFL SV GHI LVG
SV 3512 (Bâtiment SV)
Station 19
1015 Lausanne
+41 21 693 17 51
+41 21 693 17 61
Office:
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Web site: Web site: https://tronolab.epfl.ch/
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Web site: Web site: https://sv.epfl.ch/education
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Mission
Didier Trono has had a long-standing interest for interactions between viruses and their hosts. This led him to study the biology of pathogens such as human immunodeficiency virus and hepatitis B virus, and to invent a HIV-based delivery system that today is used successfully in human gene therapy. Some fifteen years ago, his research shifted towards the field of epigenetics, where he now explores the impact of retroelements and their controlling mechanisms on the development and physiology of higher organisms, including humans, and exploits this information to create new approaches for cancer diagnostic and therapeutics.Biography
After obtaining an M.D. from the University of Geneva and completing a clinical training in pathology, internal medicine and infectious diseases in Geneva and at Massachusetts General Hospital in Boston, Didier Trono embarked in a scientific career at the Whitehead Institute for Biomedical Research of MIT. In 1990, he joined the faculty of the Salk Institute for Biological Studies to launch a center for AIDS research. He moved back to Europe seven years later, before taking the reins of the newly created EPFL School of Life Sciences, which he directed from 2004 to 2012. He is now actively engaged in the efforts of Switzerland to integrate new technologies in the fields of precision medicine and personalized health.Publications
Infoscience publications
Publications
2023
* Statistical learning quantifies transposable element-mediated cis-regulation
Genome Biology. 2023. DOI : 10.1186/s13059-023-03085-7.* SARS-CoV-2 hijacks a cell damage response, which induces transcription of a more efficient Spike S-acyltransferase
Nature Communications. 2023. DOI : 10.1038/s41467-023-43027-2.* New ingredients for old recipes
Nature Genetics. 2023. DOI : 10.1038/s41588-023-01591-9.* Broadly potent anti-SARS-CoV-2 antibody shares 93% of epitope with ACE2 and provides full protection in monkeys
Journal of Infection. 2023. DOI : 10.1016/j.jinf.2023.10.008.* Take a walk on the KRAB side
Trends in Genetics. 2023. DOI : 10.1016/j.tig.2023.08.003.* Genetic features and genomic targets of human KRAB-zinc finger proteins
Genome Research. 2023. DOI : 10.1101/gr.277722.123.* Efficient and sensitive profiling of RNA–protein interactions using TLC-CLIP
Nucleic Acids Research. 2023. DOI : 10.1093/nar/gkad466.* The immunopeptidome landscape associated with T cell infiltration, inflammation and immune editing in lung cancer
Nature Cancer. 2023. DOI : 10.1038/s43018-023-00548-5.* Cryo-EM structures and binding of mouse and human ACE2 to SARS-CoV-2 variants of concern indicate that mutations enabling immune escape could expand host range
PLOS Pathogens. 2023-04-05. DOI : 10.1371/journal.ppat.1011206.2022
* Seroprevalence of anti-SARS-CoV-2 antibodies and cross-variant neutralization capacity after the Omicron BA.2 wave in Geneva, Switzerland: a population-based study
The Lancet Regional Health - Europe. 2022-12-01. DOI : 10.1016/j.lanepe.2022.100547.* A satellite DNA array barcodes chromosome 7 and regulates totipotency via ZFP819
Science Advances. 2022-10-28. DOI : 10.1126/sciadv.abp8085.* Transposon-activated POU5F1B promotes colorectal cancer growth and metastasis
Nature Communications. 2022-08-20. DOI : 10.1038/s41467-022-32649-7.* Patient-derived monoclonal antibody neutralizes SARS-CoV-2 Omicron variants and confers full protection in monkeys
Nature Microbiology. 2022-07-25. DOI : 10.1038/s41564-022-01198-6.* Transposable elements contribute to the spatiotemporal microRNA landscape in human brain development
RNA. 2022-06-22. DOI : 10.1261/rna.079100.122.* A cis-acting structural variation at the ZNF558 locus controls a gene regulatory network in human brain development
Cell Stem Cell. 2022-01-06. DOI : 10.1016/j.stem.2021.09.008.* Humoral Responses Against Variants of Concern by COVID-19 mRNA Vaccines in Immunocompromised Patients
Jama Oncology. 2022-03-10. DOI : 10.1001/jamaoncol.2022.0446.* Human reproduction is regulated by retrotransposons derived from ancient Hominidae-specific viral infections
Nature Communications. 2022-01-24. DOI : 10.1038/s41467-022-28105-1.* Microfluidic characterisation reveals broad range of SARS-CoV-2 antibody affinity in human plasma
Life Science Alliance. 2022-02-01. DOI : 10.26508/lsa.202101270.* KRAB zinc finger protein ZNF676 controls the transcriptional influence of LTR12-related endogenous retrovirus sequences
Mobile Dna. 2022-01-18. DOI : 10.1186/s13100-021-00260-0.* Occupational risk of SARS-CoV-2 infection and reinfection during the second pandemic surge: a cohort study
Occupational And Environmental Medicine. 2022. DOI : 10.1136/oemed-2021-107924.2021
* S-acylation controls SARS-CoV-2 membrane lipid organization and enhances infectivity
Developmental Cell. 2021-10-25. DOI : 10.1016/j.devcel.2021.09.016.* A highly potent antibody effective against SARS-CoV-2 variants of concern
Cell Reports. 2021-10-12. DOI : 10.1016/j.celrep.2021.109814.* Transposable elements and their KZFP controllers are drivers of transcriptional innovation in the developing human brain
Genome Research. 2021-09-01. DOI : 10.1101/gr.275133.120.* A high-throughput cell- and virus-free assay shows reduced neutralization of SARS-CoV-2 variants by COVID-19 convalescent plasma
Science Translational Medicine. 2021-08-04. DOI : 10.1126/scitranslmed.abi8452.* Large variation in anti-SARS-CoV-2 antibody prevalence among essential workers in Geneva, Switzerland
Nature Communications. 2021-06-08. DOI : 10.1038/s41467-021-23796-4.* Changes in SARS-CoV-2 Spike versus Nucleoprotein Antibody Responses Impact the Estimates of Infections in Population-Based Seroprevalence Studies
Journal Of Virology. 2021-02-01. DOI : 10.1128/JVI.01828-20.2020
* The Human RNA Helicase DDX21 Presents a Dimerization Interface Necessary for Helicase Activity
iScience. 2020-11-18. DOI : 10.1016/j.isci.2020.101811.* Endogenous retroviruses drive KRAB zinc-finger protein family expression for tumor suppression
Science Advances. 2020-10-01. DOI : 10.1126/sciadv.abc3020.* Primate-restricted KRAB zinc finger proteins and target retrotransposons control gene expression in human neurons
Science Advances. 2020-08-28. DOI : 10.1126/sciadv.aba3200.* Seroprevalence of anti-SARS-CoV-2 IgG antibodies in Geneva, Switzerland (SEROCoV-POP): a population-based study
The Lancet. 2020-06-11. DOI : 10.1016/S0140-6736(20)31304-0.* Integrated proteogenomic deep sequencing and analytics accurately identify non-canonical peptides in tumor immunopeptidomes
Nature Communications. 2020-03-10. DOI : 10.1038/s41467-020-14968-9.* KRAB-zinc finger protein gene expansion in response to active retrotransposons in the murine lineage
eLife. 2020-06-01. DOI : 10.7554/eLife.56337.* KAP1 targets actively transcribed genomic loci to exert pleomorphic effects on RNA polymerase II activity
Philosophical Transactions of the Royal Society B: Biological Sciences. 2020-02-10. DOI : 10.1098/rstb.2019.0334.2019
* DUX is a non-essential synchronizer of zygotic genome activation
Development. 2019-12-05. DOI : 10.1242/dev.177725.* KAP1 is an antiparallel dimer with a functional asymmetry
Life Science Alliance. 2019-08-01. DOI : 10.26508/lsa.201900349.* The interactome of KRAB zinc finger proteins reveals the evolutionary history of their functional diversification
The EMBO Journal. 2019-08-12. DOI : 10.15252/embj.2018101220.* ZFP30 promotes adipogenesis through the KAP1-mediated activation of a retrotransposon-derived Pparg2 enhancer
Nature Communications. 2019-04-18. DOI : 10.1038/s41467-019-09803-9.* A Dissection of Oligomerization by the TRIM28 Tripartite Motif and the Interaction with Members of the Krab-ZFP Family
Journal of Molecular Biology. 2019-06-28. DOI : 10.1016/j.jmb.2019.05.002.* Hominoid-Specific Transposable Elements and KZFPs Facilitate Human Embryonic Genome Activation and Control Transcription in Naive Human ESCs
Cell Stem Cell. 2019-03-27. DOI : 10.1016/j.stem.2019.03.012.* DPPA2 and DPPA4 are necessary to establish a 2C‐like state in mouse embryonic stem cells
EMBO reports. 2019-04-04. DOI : 10.15252/embr.201847382.* Pharmacological induction of a progenitor state for the efficient expansion of primary human hepatocytes
Hepatology. 2019. DOI : 10.1002/hep.30425.* ZNF445 is a primary regulator of genomic imprinting
Genes & Development. 2019-01-01. DOI : 10.1101/gad.320069.118.2018
* Properties of LINE-1 proteins and repeat element expression in the context of amyotrophic lateral sclerosis
Mobile Dna. 2018-12-15. DOI : 10.1186/s13100-018-0138-z.* HIV-1 Vpr and p21 restrict LINE-1 mobility
Nucleic Acids Research. 2018-07-31. DOI : 10.1093/nar/gky688.* KAP1 facilitates reinstatement of heterochromatin after DNA replication
Nucleic Acids Research. 2018-06-28. DOI : 10.1093/nar/gky580.* Individual retrotransposon integrants are differentially controlled by KZFP/KAP1-dependent histone methylation, DNA methylation and TET-mediated hydroxymethylation in naïve embryonic stem cells
Epigenetics & Chromatin. 2018-02-26. DOI : 10.1186/s13072-018-0177-1.2017
* KRAB zinc finger proteins
Development. 2017. DOI : 10.1242/dev.132605.* DUX-family transcription factors regulate zygotic genome activation in placental mammals
Nature Genetics. 2017. DOI : 10.1038/ng.3858.* Polyphenic trait promotes liver cancer in a model of epigenetic instability
Hepatology. 2017. DOI : 10.1002/hep.29182.* The mouse genome displays highly dynamic populations of KRAB-zinc finger protein genes and related genetic units
PLoS One. 2017. DOI : 10.1371/journal.pone.0173746.* KRAB zinc-finger proteins contribute to the evolution of gene regulatory networks
Nature. 2017. DOI : 10.1038/nature21683.* SMiLE-seq identifies binding motifs of single and dimeric transcription factors
Nature methods. 2017. DOI : 10.1038/nmeth.4143.2016
* Switzerland and the Digital Health Revolution
Chimia. 2016. DOI : 10.2533/chimia.2016.851.* Lentiviral vectors, two decades later
Science. 2016. DOI : 10.1126/science.aah6192.* Transposable Elements, Polydactyl Proteins, and the Genesis of Human-Specific Transcription Networks
Cold Spring Harbor symposia on quantitative biology. 2016. DOI : 10.1101/sqb.2015.80.027573.* A Large-Scale Functional Screen to Identify Epigenetic Repressors of Retrotransposon Expression
Methods in molecular biology (Clifton, N.J.). 2016. DOI : 10.1007/978-1-4939-3372-3_25.* Molecular Criteria for Defining the Naive Human Pluripotent State
Cell stem cell. 2016. DOI : 10.1016/j.stem.2016.06.011.* Transposable Elements and Their KRAB-ZFP Controllers Regulate Gene Expression in Adult Tissues
Developmental Cell. 2016. DOI : 10.1016/j.devcel.2016.02.024.* The evolution of gene expression and binding specificity of the largest transcription factor family in primates
Evolution. 2016. DOI : 10.1111/evo.12819.2015
* Venus trap in the mouse embryo reveals distinct molecular dynamics underlying specification of first embryonic lineages
EMBO reports. 2015. DOI : 10.15252/embr.201540162.* Release of human cytomegalovirus from latency by a KAP1/TRIM28 phosphorylation switch
eLife. 2015. DOI : 10.7554/eLife.06068.* A KAP1 phosphorylation switch controls MyoD function during skeletal muscle differentiation
Genes & development. 2015. DOI : 10.1101/gad.254532.114.* TRIM28 Represses Transcription of Endogenous Retroviruses in Neural Progenitor Cells
Cell Reports. 2015. DOI : 10.1016/j.celrep.2014.12.004.2014
* As Time Goes by: KRABs Evolve to KAP Endogenous Retroelements
Developmental Cell. 2014. DOI : 10.1016/j.devcel.2014.10.019.* Identification of the transcription factor ZEB1 as a central component of the adipogenic gene regulatory network
Elife. 2014. DOI : 10.7554/eLife.03346.* Dual-regulated Lentiviral Vector for Gene Therapy of X-linked Chronic Granulomatosis
Molecular Therapy. 2014. DOI : 10.1038/mt.2014.87.* Evolutionally dynamic L1 regulation in embryonic stem cells
Genes & development. 2014. DOI : 10.1101/gad.241661.114.* Interplay of TRIM28 and DNA methylation in controlling human endogenous retroelements
Genome research. 2014. DOI : 10.1101/gr.172833.114.* Loss of transcriptional control over endogenous retroelements during reprogramming to pluripotency
Genome research. 2014. DOI : 10.1101/gr.172809.114.* Control of mitophagie by microRNAs - A key step of erythropoiesis
M S-Medecine Sciences. 2014. DOI : 10.1051/medsci/20143001003.2013
* VEGFR-3 Neutralization Inhibits Ovarian Lymphangiogenesis, Follicle Maturation, and Murine Pregnancy
American Journal Of Pathology. 2013. DOI : 10.1016/j.ajpath.2013.07.031.* Prototype Foamy Virus Bet Impairs the Dimerization and Cytosolic Solubility of Human APOBEC3G
Journal of virology. 2013. DOI : 10.1128/JVI.03385-12.* A switch between topological domains underlies HoxD genes collinearity in mouse limbs
Science (New York, N.Y.). 2013. DOI : 10.1126/science.1234167.* Global and stage specific patterns of krüppel-associated-box zinc finger protein gene expression in murine early embryonic cells
PloS One. 2013. DOI : 10.1371/journal.pone.0056721.* Peripuberty stress leads to abnormal aggression, altered amygdala and orbitofrontal reactivity and increased prefrontal MAOA gene expression
Transl Psychiatry. 2013. DOI : 10.1038/tp.2012.144.* De novo DNA methylation of endogenous retroviruses is shaped by KRAB-ZFPs/KAP1 and ESET
Development (Cambridge, England). 2013. DOI : 10.1242/dev.087585.* TRIM28 repression of retrotransposon-based enhancers is necessary to preserve transcriptional dynamics in embryonic stem cells
Genome research. 2013. DOI : 10.1101/gr.147678.112.2012
* The KRAB-ZFP/KAP1 System Contributes to the Early Embryonic Establishment of Site-Specific DNA Methylation Patterns Maintained during Development
Cell Reports. 2012. DOI : 10.1016/j.celrep.2012.08.043.* KAP1 regulates gene networks controlling T-cell development and responsiveness
FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2012. DOI : 10.1096/fj.12-206177.* MicroRNA-124 Is a Subventricular Zone Neuronal Fate Determinant
The Journal of neuroscience : the official journal of the Society for Neuroscience. 2012. DOI : 10.1523/JNEUROSCI.0558-12.2012.* Embryonic stem cell potency fluctuates with endogenous retrovirus activity
Nature. 2012. DOI : 10.1038/nature11244.* Liver-specific ablation of Krüppel-associated box-associated protein 1 in mice leads to male-predominant hepatosteatosis and development of liver adenoma
Hepatology. 2012. DOI : 10.1002/hep.25767.* The Kruppel-associated Box Repressor Domain Can Induce Reversible Heterochromatization of a Mouse Locus in Vivo
The Journal of biological chemistry. 2012. DOI : 10.1074/jbc.M112.350884.* Gene therapy: too much splice can spoil the dish
The Journal of clinical investigation. 2012. DOI : 10.1172/JCI63066.* KAP1 regulates gene networks controlling mouse B-lymphoid cell differentiation and function
Blood. 2012. DOI : 10.1182/blood-2011-12-401117.* The ATM substrate KAP1 controls DNA repair in heterochromatin: Regulation by HP1 proteins and Serine 473/824 phosphorylation
Molecular cancer research : MCR. 2012. DOI : 10.1158/1541-7786.MCR-11-0134.2011
* A novel lentiviral vector targets gene transfer into human hematopoietic stem cells in marrow from patients with bone marrow failure syndrome and in vivo in humanized mice
Blood. 2011. DOI : 10.1182/blood-2011-04-346619.* In Embryonic Stem Cells, ZFP57/KAP1 Recognize a Methylated Hexanucleotide to Affect Chromatin and DNA Methylation of Imprinting Control Regions
Molecular Cell. 2011. DOI : 10.1016/j.molcel.2011.08.032.* A gene-rich, transcriptionally active environment and the pre-deposition of repressive marks are predictive of susceptibility to KRAB/KAP1-mediated silencing
BMC genomics. 2011. DOI : 10.1186/1471-2164-12-378.* Measuring In Vivo Protein Half-Life
Chemistry & Biology. 2011. DOI : 10.1016/j.chembiol.2011.03.014.* Lentiviral Vector Mediated Transgenesis
Current Protocols in Mouse Biology 1. 2011. DOI : 10.1002/9780470942390.mo100169.* Chromosome Conformation Capture Uncovers Potential Genome-Wide Interactions between Human Conserved Non-Coding Sequences
PloS One. 2011. DOI : 10.1371/journal.pone.0017634.* Profaning the Ultimate Sanctuary: HIV Latency in Hematopoietic Stem Cells
Cell host & microbe. 2011. DOI : 10.1016/j.chom.2011.03.001.* Dynamic control of endogenous retroviruses during development
Virology. 2011. DOI : 10.1016/j.virol.2010.12.007.2010
* Homology-based identification of capsid determinants that protect HIV1 from human TRIM5α restriction
The Journal of biological chemistry. 2010. DOI : 10.1074/jbc.M110.187609.* A systematic enhancer screen using lentivector transgenesis identifies conserved and non-conserved functional elements at the olig1 and olig2 locus
PloS One. 2010. DOI : 10.1371/journal.pone.0015741.* Structure-function analysis points to a polynucleotide-accommodating groove essential for APOBEC3A restriction activities
Journal of virology. 2010. DOI : 10.1128/JVI.01651-10.* Inducible Gene and shRNA Expression in Resident Hematopoietic Stem Cells In Vivo
Stem cells (Dayton, Ohio). 2010. DOI : 10.1002/stem.460.* Genetic Reactivation of Cone Photoreceptors Restores Visual Responses in Retinitis pigmentosa
Science (New York, N.Y.). 2010. DOI : 10.1126/science.1190897.* KRAB-Zinc Finger Proteins and KAP1 Can Mediate Long-Range Transcriptional Repression through Heterochromatin Spreading
PLoS genetics. 2010. DOI : 10.1371/journal.pgen.1000869.* The specificity of TRIM5{alpha}-mediated restriction is influenced by its coiled-coil domain
Journal of virology. 2010. DOI : 10.1128/JVI.02413-09.* KAP1 controls endogenous retroviruses in embryonic stem cells
Nature. 2010. DOI : 10.1038/nature08674.2009
* Biosafety in ex vivo gene therapy and conditional ablation of lentivirally transduced hepatocytes in nonhuman primates
Molecular therapy : the journal of the American Society of Gene Therapy. 2009. DOI : 10.1038/mt.2009.143.* Regulation of Episomal Gene Expression by KRAB/KAP1-Mediated Histone Modifications
Journal of Virology. 2009. DOI : 10.1128/JVI.00001-09.2008
* Antiprion prophylaxis by gene transfer of a soluble prion antagonist
American journal of pathology. 2008. DOI : 10.2353/ajpath.2008.070836.* KAP1-mediated epigenetic repression in the forebrain modulates behavioral vulnerability to stress
Neuron. 2008. DOI : 10.1016/j.neuron.2008.09.036.* A human TRIM5alpha B30.2/SPRY domain mutant gains the ability to restrict and prematurely uncoat B-tropic murine leukemia virus
Virology. 2008. DOI : 10.1016/j.virol.2008.05.008.* Characterization of APOBEC3G binding to 7SL RNA
Retrovirology. 2008. DOI : 10.1186/1742-4690-5-54.* Genotypic features of lentivirus transgenic mice
Journal of virology. 2008. DOI : 10.1128/JVI.00623-08.* APOBEC3-independent interferon-induced viral clearance in hepatitis B virus transgenic mice
Journal of Virology. 2008. DOI : 10.1128/JVI.00216-08.2007
* Model Structure of Human APOBEC3G
PLoS ONE. 2007. DOI : 10.1371/journal.pone.0000378.* Lentivirus as a tool for lineage-specific gene manipulations
genesis. 2007. DOI : 10.1002/dvg.20313.* Molecular mechanism of hepcidin deficiency in a patient with juvenile hemochromatosis
Haematologica. 2007. DOI : 10.3324/haematol.10545.* Inhibition of HIV-1 multiplication by a modified U7 snRNA inducing Tat and Rev exon skipping
The journal of gene medicine. 2007. DOI : 10.1002/jgm.1027.* Expression of FGF-2 in neural progenitor cells enhances their potential for cellular brain repair in the rodent cortex
Brain : a journal of neurology. 2007. DOI : 10.1093/brain/awm200.* Interfering residues narrow the spectrum of MLV restriction by human TRIM5alpha
PLOS Pathogens. 2007. DOI : 10.1371/journal.ppat.0030200.2006
* Ataxia-telangiectasia-mutated (ATM) protein can enhance human immunodeficiency virus type 1 replication by stimulating Rev function
Journal of Virology. 2006. DOI : 10.1128/JVI.80.5.2445-2452.2006.* Ex vivo lentivirus transduction and immediate transplantation of uncultured hepatocytes for treating hyperbilirubinemic Gunn rat
Transplantation. 2006. DOI : 10.1097/01.tp.0000234675.56598.35.* Multipotential nestin and Isl-1 positive mesenchymal stem cells isolated from human pancreatic islets
Biochemical and Biophysical Research Communications. 2006. DOI : 10.1016/j.bbrc.2006.05.016.* A versatile tool for conditional gene expression and knockdown
Nature Methods. 2006. DOI : 10.1038/nmeth846.* Tuning silence: conditional systems for RNA interference
Nature Methods. 2006. DOI : 10.1038/nmeth914.2005
* A simple and highly effective method for the stable transduction of uncultured porcine hepatocytes using lentiviral vector
Cell Transplantation. 2005. DOI : 10.3727/000000005783982828.* Therapeutic lentivirus-mediated neonatal in vivo gene therapy in hyperbilirubinemic Gunn rats
Molecular therapy : the journal of the American Society of Gene Therapy. 2005. DOI : 10.1016/j.ymthe.2005.06.482.* Lentiviral vectors and antiretroviral intrinsic immunity
Human Gene Therapy. 2005. DOI : 10.1089/hum.2005.16.913.* Harnessing HIV for therapy, basic research and biotechnology
Trends Biotechnol. 2005. DOI : 10.1016/j.tibtech.2004.11.001.* Treatment of fulminant liver failure by transplantation of microencapsulated primary or immortalized xenogeneic hepatocytes
Transplant Proc. 2005. DOI : 10.1016/j.transproceed.2005.01.017.* Editing at the crossroad of innate and adaptive immunity
Science. 2005. DOI : 10.1126/science.1105964.* DNA damage sensors ATM, ATR, DNA-PKcs, and PARP-1 are dispensable for human immunodeficiency virus type 1 integration
Journal of Virology. 2005. DOI : 10.1128/JVI.79.5.2973-2978.2005.* Development of cellular models for ribosomal protein S19 (RPS19)-deficient diamond-blackfan anemia using inducible expression of siRNA against RPS19
Molecular Therapy. 2005. DOI : 10.1016/j.ymthe.2004.12.001.* Statins reduce interleukin-6-induced C-reactive protein in human hepatocytes: new evidence for direct antiinflammatory effects of statins
Arterioscler Thromb Vasc Biol. 2005. DOI : 10.1161/01.ATV.0000163840.63685.0c.* Deficiency of ribosomal protein S19 in CD34+ cells generated by siRNA blocks erythroid development and mimics defects seen in Diamond-Blackfan anemia
Blood. 2005. DOI : 10.1182/blood-2004-08-3115.* Transduction of CpG DNA-stimulated primary human B cells with bicistronic lentivectors
Molecular Therapy. 2005. DOI : 10.1016/j.ymthe.2005.05.010.* Novel lentiviral vectors displaying "early acting cytokines" selectively promote survival and transduction of NOD/SCID repopulating human hematopoietic stem cells
Blood. 2005. DOI : 10.1182/blood-2004-12-4736.* Oncogenesis Following Delivery of a Nonprimate Lentiviral Gene Therapy Vector to Fetal and Neonatal Mice
Molecular Therapy. 2005. DOI : 10.1016/j.ymthe.2005.07.358.2004
* A single amino acid determinant governs the species-specific sensitivity of APOBEC3G to Vif action
Journal of Biological Chemistry. 2004. DOI : 10.1074/jbc.C400060200.* Response to Comment on "Inhibition of Hepatitis B Virus Replication by APOBEC3G"
Science. 2004. DOI : 10.1126/science.1101974.* Inhibition of hepatitis B virus replication by APOBEC3G
Science. 2004. DOI : 10.1126/science.1092066.* Contribution of proteoglycans to human immunodeficiency virus type 1 brain invasion
Journal of Virology. 2004. DOI : 10.1128/JVI.78.12.6567-6584.2004.* ARF1 regulates Nef-induced CD4 degradation
Current Biology. 2004. DOI : 10.1016/j.cub.2004.06.021.* Retroviruses under editing crossfire: a second member of the human APOBEC3 family is a Vif-blockable innate antiretroviral factor
EMBO Rep. 2004. DOI : 10.1038/sj.embor.7400192.* Entry and transcription as key determinants of differences in CD4 T-cell permissiveness to human immunodeficiency virus type 1 infection
Journal of Virology. 2004. DOI : 10.1128/JVI.78.19.10747-10754.2004.* Lentivirus-mediated RNA interference of DC-SIGN expression inhibits human immunodeficiency virus transmission from dendritic cells to T cells
Journal of Virology. 2004. DOI : 10.1128/JVI.78.20.10848-10855.2004.* The innate antiretroviral factor APOBEC3G does not affect human LINE-1 retrotransposition in a cell culture assay
Journal of Biological Chemistry. 2004. DOI : 10.1074/jbc.C400334200.* Lentiviral vectors interfering with virus-induced CD4 down-modulation potently block human immunodeficiency virus type 1 replication in primary lymphocytes
Journal of Virology. 2004. DOI : 10.1128/JVI.78.23.13072-13081.2003
* Lentivirus-mediated transduction of connexin cDNAs shows level- and isoform-specific alterations in insulin secretion of primary pancreatic beta-cells
Journal of Cell Science. 2003. DOI : 10.1242/jcs.00442.* Hide, shield and strike back: how HIV-infected cells avoid immune eradication
Nat Rev Immunol. 2003. DOI : 10.1038/nri998.* Lentiviral vector transduction of NOD/SCID repopulating cells results in multiple vector integrations per transduced cell: risk of insertional mutagenesis
Blood. 2003. DOI : 10.1182/blood-2002-07-2238.* Efficient transduction of primary human B lymphocytes and nondividing myeloma B cells with HIV-1-derived lentiviral vectors
Blood. 2003. DOI : 10.1182/blood-2001-12-0249.* IL-7 surface-engineered lentiviral vectors promote survival and efficient gene transfer in resting primary T lymphocytes
Blood. 2003. DOI : 10.1182/blood-2002-07-2224.* Activity analysis of housekeeping promoters using self-inactivating lentiviral vector delivery into the mouse retina
Gene Therapy. 2003. DOI : 10.1038/sj.gt.3301948.* Ectopic expression of the beta-cell specific transcription factor Pdx1 inhibits glucagon gene transcription
Diabetologia. 2003. DOI : 10.1007/s00125-003-1115-7.* Virology. Picking the right spot.
Science. 2003. DOI : 10.1126/science.1086238.* Broad antiretroviral defence by human APOBEC3G through lethal editing of nascent reverse transcripts
Nature. 2003. DOI : 10.1038/nature01709.* Conditional suppression of cellular genes: lentivirus vector-mediated drug-inducible RNA interference
Journal of Virology. 2003. DOI : 10.1128/JVI.77.16.8957-8951.2003.* Lentivector-mediated transfer of Bmi-1 and telomerase in muscle satellite cells yields a duchenne myoblast cell line with long-term genotypic and phenotypic stability
Human Gene Therapy. 2003. DOI : 10.1089/104303403322495034.2002
* Efficient gene transfer into human primary blood lymphocytes by surface-engineered lentiviral vectors that display a T cell-activating polypeptide
Blood. 2002. DOI : 10.1182/blood.V99.7.2342.* Differentiation of the RN33B cell line into forebrain projection neurons after transplantation into the neonatal rat brain
Exp Neurol. 2002. DOI : 10.1006/exnr.2002.7888.* Transepithelial transport of HIV-1 by M cells is receptor-mediated
Proceedings Of The National Academy Of Sciences Of The United States Of America. 2002. DOI : 10.1073/pnas.142586899.* Highly efficient lentiviral vector-mediated transduction of nondividing, fully reimplantable primary hepatocytes
Molecular Therapy. 2002. DOI : 10.1006/mthe.2002.0653.* Lentiviral vectors pseudotyped with a modified RD114 envelope glycoprotein show increased stability in sera and augmented transduction of primary lymphocytes and CD34+ cells derived from human and nonhuman primates
Blood. 2002. DOI : 10.1182/blood-2001-11-0042.* Modalities of interleukin-7-induced human immunodeficiency virus permissiveness in quiescent T lymphocytes
Journal of Virology. 2002. DOI : 10.1128/JVI.76.18.9103-9111.2002.* Role for human immunodeficiency virus type 1 membrane cholesterol in viral internalization
Journal of Virology. 2002. DOI : 10.1128/JVI.76.20.10356-10364.2002.* The HIV-1 Nef protein and phagocyte NADPH oxidase activation
Journal of Biological Chemistry. 2002. DOI : 10.1074/jbc.M200862200.2001
* Living in oblivion: HIV immune evasion
Semin Immunol. 2001. DOI : 10.1006/smim.2000.0295.* Cytoplasmic recruitment of INI1 and PML on incoming HIV preintegration complexes: interference with early steps of viral replication
Molecular Cell. 2001. DOI : 10.1016/S1097-2765(01)00255-6.* Transduction of CD34+ cells with lentiviral vectors enables the production of large quantities of transgene-expressing immature and mature dendritic cells
J Gene Med. 2001. DOI : 10.1002/1521-2254(200107/08)3:4<311::AID-JGM198>3.0.CO;2-B.* Inhibition of HIV-1 in cell culture by oligonucleotide-loaded nanoparticles
Pharm Res. 2001. DOI : 10.1023/A:1010962507273.* Organ distribution of gene expression after intravenous infusion of targeted and untargeted lentiviral vectors
Gene Therapy. 2001. DOI : 10.1038/sj.gt.3301552.2000
* Lentivirus vector gene expression during ES cell-derived hematopoietic development in vitro
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Journal of Cell Biology. 1997. DOI : 10.1083/jcb.139.1.37.Teaching & PhD
Teaching
Life Sciences Engineering
PhD Students
Coudray Alexandre, Kasa Keida, Milovanovic Danica, Pulver Cyril David Son-Tuyên,Past EPFL PhD Students
Bojkowska Karolina , Brandão Sanches Vong Martins Filipe Amândio , Bulliard Yannick , Coluccio Andrea , Corsinotti Andrea , De Tribolet-Hardy Jonas Caspar , Ecco Gabriela , Groner Anna Claire , Helleboid Pierre-Yves Joseph Laurent , Iouranova Alexandra , Jost Stéphanie , Kauzlaric Annamaria , Maillard Pierre , Marzetta Flavia , Meylan Sylvain , Pennese Natali , Sauvain Marc-Olivier , Shin Eunji , Thorball Christian Axel Wandall ,Courses
New tools & research strategies in personalized health
We will define the concept of personalized health, describe the underlying technologies, the technological, legal and ethical challenges that the field faces today, and how they are being met.