Florence Pojer

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florence.pojer@epfl.ch +41 21 693 19 76 https://www.epfl.ch/research/facilities/ptpsp/

EPFL SV PTECH PTPSP
AI 2147 (Bâtiment AI)
Station 19
CH-1015 Lausanne

+41 21 693 19 76
+41 21 693 09 04
Local: AI 2147
EPFL > SV > PTECH > PTPSP

Web site: Site web: https://www.epfl.ch/research/facilities/ptpsp/

EPFL AVP-PGE EDMS-ENS
SV 2515 (Bâtiment SV)
Station 19
CH-1015 Lausanne

+41 21 693 19 76
Local: SV 2515
EPFL > VPA > VPA-AVP-PGE > AVP-PGE-EDOC > EDMS-ENS

Web site: Site web: https://go.epfl.ch/edms

EPFL VPRHO DSPS COSEC-SV
AI 2147 (Bâtiment AI)
Station 19
CH-1015 Lausanne

+41 21 693 19 76
Local: AI 2147
EPFL > VPO > VPO-SE > OHS > COSEC-SV

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Données administratives

Domaines de compétences

Protein Production
Protein Purification
X-ray Crystallography
Cryo-EM
Integrative Structural Biology
Structure-based Drug Design
Biophysical Techniques
Microbiology

Parcours professionnel

Head of Protein Crystallography Core Facility

SV-EPFL

2011-2017

Staff scientist

Chair of Microbial Pathogenesis, Prof. Stewart Cole

EPFL

2007-2018

Post-doc

Structural Biology lab, Prof. Joseph Noel

The Salk Institute (La Jolla, USA)

2004-2007

Formation

PhD thesis

Antibiotic research

Tuebingen (Germany)

1999-2003

Pharm D

Pharmacy School

Besancon (France)

1992-1998

Publications

Publications Infoscience

A highly potent antibody effective against SARS-CoV-2 variants of concern

C. Fenwick; P. Turelli; L. Perez; C. Pellaton; L. Esteves-Leuenberger et al.

Cell Reports. 2021-10-12. DOI : 10.1016/j.celrep.2021.109814.

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A high-throughput cell- and virus-free assay shows reduced neutralization of SARS-CoV-2 variants by COVID-19 convalescent plasma

C. Fenwick; P. Turelli; C. Pellaton; A. Farina; J. Campos et al.

Science Translational Medicine. 2021-08-04. DOI : 10.1126/scitranslmed.abi8452.

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Palmitoylated acyl protein thioesterase APT2 deforms membranes to extract substrate acyl chains

L. Abrami; M. Audagnotto; S. Ho; M. J. Marcaida; F. S. Mesquita et al.

Nature Chemical Biology. 2021-04-01. DOI : 10.1038/s41589-021-00753-2.

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Azole-Based Indoleamine 2,3-Dioxygenase 1 (IDO1) Inhibitors

U. F. Rohrig; S. R. Majjigapu; A. Reynaud; F. Pojer; N. Dilek et al.

Journal Of Medicinal Chemistry. 2021-02-25. DOI : 10.1021/acs.jmedchem.0c01968.

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Changes in SARS-CoV-2 Spike versus Nucleoprotein Antibody Responses Impact the Estimates of Infections in Population-Based Seroprevalence Studies

C. Fenwick; A. Croxatto; A. T. Coste; F. Pojer; C. Andre et al.

Journal Of Virology. 2021-02-01. DOI : 10.1128/JVI.01828-20.

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Structural investigation of ACE2 dependent disassembly of the trimeric SARS-CoV-2 Spike glycoprotein

D. Ni; K. Lau; F. Lehmann; A. Fränkl; D. Hacker et al.

bioRxiv. 2020-10-12. DOI : 10.1101/2020.10.12.336016.

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Selective inhibition of STAT3 signaling using monobodies targeting the coiled-coil and N-terminal domains

G. La Sala; C. Michiels; T. Kuekenshoener; T. Brandstoetter; B. Maurer et al.

Nature Communications. 2020-08-17. DOI : 10.1038/s41467-020-17920-z.

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High resolution CryoEM structure of the ring-shaped virulence factor EspB from Mycobacterium tuberculosis

J. Piton; F. Pojer; S. Wakatsuki; C. Gati; S. Cole

Journal of Structural Biology: X. 2020-07-02. DOI : 10.1016/j.yjsbx.2020.100029.

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De novo development of proteolytically resistant therapeutic peptides for oral administration

X.-D. Kong; J. Moriya; V. Carle; F. Pojer; L. A. Abriata et al.

Nature Biomedical Engineering. 2020-05-11. DOI : 10.1038/s41551-020-0556-3.

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Btk SH2-kinase interface is critical for allosteric kinase activation and its targeting inhibits B-cell neoplasms

D. P. Duarte; A. J. Lamontanara; G. La Sala; S. Jeong; Y.-K. Sohn et al.

Nature Communications. 2020-05-08. DOI : 10.1038/s41467-020-16128-5.

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Inhibition Mechanisms of Indoleamine 2,3-Dioxygenase 1 (IDO1)

U. F. Rohrig; A. Reynaud; S. R. Majjigapu; P. Vogel; F. Pojer et al.

Journal Of Medicinal Chemistry. 2019-10-10. DOI : 10.1021/acs.jmedchem.9b00942.

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Structural and functional dissection of the DH and PH domains of oncogenic Bcr-Abl tyrosine kinase

S. Reckel; C. Gehin; D. Tardivon; S. Georgeon; T. Kukenshoner et al.

Nature Communications. 2017. DOI : 10.1038/s41467-017-02313-6.

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Selective Targeting of SH2 Domain–Phosphotyrosine Interactions of Src Family Tyrosine Kinases with Monobodies

T. Kükenshöner; N. E. Schmit; E. Bouda; F. Sha; F. Pojer et al.

Journal of Molecular Biology. 2017. DOI : 10.1016/j.jmb.2017.03.023.

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A rheostat mechanism governs the bifurcation of carbon flux in mycobacteria

P. Murima; M. Zimmermann; T. Chopra; F. Pojer; G. Fonti et al.

Nature Communications. 2016. DOI : 10.1038/ncomms12527.

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Discovery of benzothiazoles as antimycobacterial agents: Synthesis, structure-activity relationships and binding studies with Mycobacterium tuberculosis decaprenylphosphoryl-beta-D-ribose 2 '-oxidase

S. Landge; A. B. Mullick; K. Nagalapur; J. Neres; V. Subbulakshmi et al.

Bioorganic & Medicinal Chemistry. 2015. DOI : 10.1016/j.bmc.2015.11.017.

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Lansoprazole is an antituberculous prodrug targeting cytochrome bc1

J. Rybniker; A. Vocat; C. Sala; P. Busso; F. Pojer et al.

Nature Communications. 2015. DOI : 10.1038/ncomms8659.

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Structure of EspB, a secreted substrate of the ESX-1 secretion system of Mycobacterium tuberculosis

N. Korotkova; J. Piton; J. M. Wagner; S. Boy-Roettger; A. Japaridze et al.

Journal Of Structural Biology. 2015. DOI : 10.1016/j.jsb.2015.06.003.

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2-Carboxyquinoxalines Kill Mycobacterium tuberculosis through Noncovalent Inhibition of DprE1

J. Neres; R. C. Hartkoorn; L. R. Chiarelli; R. Gadupudi; M. R. Pasca et al.

Acs Chemical Biology. 2015. DOI : 10.1021/cb5007163.

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Internalization and vacuolar targeting of the brassinosteroid hormone receptor BRI1 are regulated by ubiquitination

S. Martins; E. M. N. Dohmann; A. Cayrel; A. Johnson; W. Fischer et al.

Nature Communications. 2015. DOI : 10.1038/ncomms7151.

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The Crystal Structures of Apo and cAMP-Bound GlxR from Corynebacterium glutamicum Reveal Structural and Dynamic Changes upon cAMP Binding in CRP/FNR Family Transcription Factors

P. D. Townsend; B. Jungwirth; F. Pojer; M. Bussmann; V. A. Money et al.

Plos One. 2014. DOI : 10.1371/journal.pone.0113265.

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Dithiol amino acids can structurally shape and enhance the ligand-binding properties of polypeptides

S. Chen; R. Gopalakrishnan; T. Schaer; F. Marger; R. Hovius et al.

Nature Chemistry. 2014. DOI : 10.1038/nchem.2043.

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The cysteine desulfurase IscS of Mycobacterium tuberculosis is involved in iron sulfur cluster biogenesis and oxidative stress defence

J. Rybniker; F. Pojer; J. Marienhagen; G. S. Kolly; J. M. Chen et al.

Biochemical Journal. 2014. DOI : 10.1042/Bj20130732.

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Functional Dissection of Intersubunit Interactions in the EspR Virulence Regulator of Mycobacterium tuberculosis

B. Blasco; A. Japaridze; M. Stenta; B. I. M. Wicky; G. Dietler et al.

Journal Of Bacteriology. 2014. DOI : 10.1128/Jb.00039-1.

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Towards a new combination therapy for tuberculosis with next generation benzothiazinones

V. Makarov; B. Lechartier; M. Zhang; J. de Almeida Neres; J. Pedro et al.

Embo Molecular Medicine. 2014. DOI : 10.1002/emmm.201303575.

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Pyridomycin bridges the NADH- and substratebinding pockets of the enoyl reductase InhA

R. C. Hartkoorn; F. Pojer; J. A. Read; H. Gingell; J. Neres et al.

Nature Chemical Biology. 2014. DOI : 10.1038/nchembio.1405.

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Peptide ligands stabilized by small molecules

S. Chen; D. Bertoldo; A. Angelini; F. Pojer; C. Heinis

Angewandte Chemie (International ed. in English). 2014. DOI : 10.1002/anie.201309459.

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Structure and Function of Essential Components of Contractile Injection Systems

S. Buth

Lausanne, EPFL, 2014. DOI : 10.5075/epfl-thesis-5939.

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Phenotypic Profiling of Mycobacterium tuberculosis EspA Point Mutants Reveals that Blockage of ESAT-6 and CFP-10 Secretion In Vitro Does Not Always Correlate with Attenuation of Virulence

J. M. Chen; M. Zhang; J. Rybniker; L. Basterra; N. Dhar et al.

Journal Of Bacteriology. 2013. DOI : 10.1128/Jb.00967-13.

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Mycobacterium tuberculosis EspB binds phospholipids and mediates EsxA-independent virulence

J. M. Chen; M. Zhang; J. Rybniker; S. Boy-Röttger; N. Dhar et al.

Molecular Microbiology. 2013. DOI : 10.1111/mmi.12336.

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Tetrahydrobiopterin Biosynthesis as an Off-Target of Sulfa Drugs

H. Haruki; M. G. Pedersen; K. I. Gorska; F. Pojer; K. Johnsson

Science. 2013. DOI : 10.1126/science.1232972.

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Structural Basis for Benzothiazinone-Mediated Killing of Mycobacterium tuberculosis

J. Neres; F. Pojer; E. Molteni; L. R. Chiarelli; N. Dhar et al.

Science Translational Medicine. 2012. DOI : 10.1126/scitranslmed.3004395.

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Towards a new tuberculosis drug: pyridomycin - nature's isoniazid

R. C. Hartkoorn; C. Sala; J. Neres; F. Pojer; S. Magnet et al.

Embo Molecular Medicine. 2012. DOI : 10.1002/emmm.201201689.

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Characterization of Molecular Determinants of the Conformational Stability of Macrophage Migration Inhibitory Factor: Leucine 46 Hydrophobic Pocket

F. El-Turk; B. Fauvet; A. Ashrafi; H. Ouertatani-Sakouhi; M.-K. Cho et al.

PLoS ONE. 2012. DOI : 10.1371/journal.pone.0045024.

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Virulence Regulator EspR of Mycobacterium tuberculosis Is a Nucleoid-Associated Protein

B. Blasco; J. M. Chen; R. Hartkoorn; C. Sala; S. Uplekar et al.

Plos Pathogens. 2012. DOI : 10.1371/journal.ppat.1002621.

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Directed evolution of the suicide protein O⁶-alkylguanine-DNA alkyltransferase for increased reactivity results in an alkylated protein with exceptional stability

B. Mollwitz; E. Brunk; S. Schmitt; F. Pojer; M. Bannwarth et al.

Biochemistry. 2012. DOI : 10.1021/bi2016537.

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Structure and function of the transketolase from Mycobacterium tuberculosis and comparison with the human enzyme

E. Fullam; F. Pojer; T. Bergfors; T. A. Jones; S. T. Cole

Open Biology. 2012. DOI : 10.1098/rsob.110026.

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Benzothiazinones Are Suicide Inhibitors of Mycobacterial Decaprenylphosphoryl-β-

C. Trefzer; H. Škovierová; S. Buroni; A. Bobovská; S. Nenci et al.

Journal of the American Chemical Society. 2012. DOI : 10.1021/ja211042r.

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EspD Is Critical for the Virulence-Mediating ESX-1 Secretion System in Mycobacterium tuberculosis

J. M. Chen; S. Boy-Röttger; N. Dhar; N. Sweeney; R. S. Buxton et al.

Journal of bacteriology. 2011. DOI : 10.1128/JB.06417-11.

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Atypical DNA recognition mechanism used by the EspR virulence regulator of Mycobacterium tuberculosis

B. Blasco; M. Stenta; L. Alonso-Sarduy; G. Dietler; M. Dal Peraro et al.

Molecular Microbiology. 2011. DOI : 10.1111/j.1365-2958.2011.07813.x.

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Towards anti-virulence drugs targeting ESX-1 mediated pathogenesis of Mycobacterium tuberculosis

J. M. Chen; F. Pojer; B. Blasco; S. T. Cole

Drug Discovery Today: Disease Mechanisms. 2010. DOI : 10.1016/j.ddmec.2010.09.002.

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Sigma Factor F does not Prevent Rifampin Inhibition of RNA Polymerase or Cause Rifampin Tolerance in Mycobacterium tuberculosis

R. C. Hartkoorn; C. Sala; S. J. Magnet; J. M. Chen; F. Pojer et al.

Journal of bacteriology. 2010. DOI : 10.1128/JB.00687-10.

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A finite element model of the LHC dipole cold mass with hysteretic, non-linear behavior and single turn description

M. Pojer

Lausanne, EPFL, 2009. DOI : 10.5075/epfl-thesis-4259.

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Enseignement & Phd

Cours

Integrative structural biology for Life sciences

Recombinant protein expression in animal cells for applications in medicine and structural biology

Methods: from disease models to therapy

Ce cours décrira les méthodes qui sont à la base des approches translationnelles depuis la génération et la caractérisation des modèles des maladies jusqu'aux applications thérapeutiques. Les bases théoriques seront complétées par des rotations pratiques dans les plateformes technologiques.

Methods: omics in biomedical research

Les méthodologies à haut débit, appelées "Omiques", permettent de caractériser la complexité et la dynamique de tout système biologique. Ce cours fournira une description générale des méthodes liées au domaine des "Omiques", suivie de rotations pratiques dans les plateformes participantes.

Florence Pojer

Responsable d'unité

Domaines de compétences

Parcours professionnel

Formation

Publications

Publications Infoscience

A highly potent antibody effective against SARS-CoV-2 variants of concern

A high-throughput cell- and virus-free assay shows reduced neutralization of SARS-CoV-2 variants by COVID-19 convalescent plasma

Palmitoylated acyl protein thioesterase APT2 deforms membranes to extract substrate acyl chains

Azole-Based Indoleamine 2,3-Dioxygenase 1 (IDO1) Inhibitors

Changes in SARS-CoV-2 Spike versus Nucleoprotein Antibody Responses Impact the Estimates of Infections in Population-Based Seroprevalence Studies

Structural investigation of ACE2 dependent disassembly of the trimeric SARS-CoV-2 Spike glycoprotein

Selective inhibition of STAT3 signaling using monobodies targeting the coiled-coil and N-terminal domains

High resolution CryoEM structure of the ring-shaped virulence factor EspB from Mycobacterium tuberculosis

De novo development of proteolytically resistant therapeutic peptides for oral administration

Btk SH2-kinase interface is critical for allosteric kinase activation and its targeting inhibits B-cell neoplasms

Inhibition Mechanisms of Indoleamine 2,3-Dioxygenase 1 (IDO1)

Structural and functional dissection of the DH and PH domains of oncogenic Bcr-Abl tyrosine kinase

Selective Targeting of SH2 Domain–Phosphotyrosine Interactions of Src Family Tyrosine Kinases with Monobodies

A rheostat mechanism governs the bifurcation of carbon flux in mycobacteria

Discovery of benzothiazoles as antimycobacterial agents: Synthesis, structure-activity relationships and binding studies with Mycobacterium tuberculosis decaprenylphosphoryl-beta-D-ribose 2 '-oxidase

Lansoprazole is an antituberculous prodrug targeting cytochrome bc1

Structure of EspB, a secreted substrate of the ESX-1 secretion system of Mycobacterium tuberculosis

2-Carboxyquinoxalines Kill Mycobacterium tuberculosis through Noncovalent Inhibition of DprE1

Internalization and vacuolar targeting of the brassinosteroid hormone receptor BRI1 are regulated by ubiquitination

The Crystal Structures of Apo and cAMP-Bound GlxR from Corynebacterium glutamicum Reveal Structural and Dynamic Changes upon cAMP Binding in CRP/FNR Family Transcription Factors

Dithiol amino acids can structurally shape and enhance the ligand-binding properties of polypeptides

The cysteine desulfurase IscS of Mycobacterium tuberculosis is involved in iron sulfur cluster biogenesis and oxidative stress defence

Functional Dissection of Intersubunit Interactions in the EspR Virulence Regulator of Mycobacterium tuberculosis

Towards a new combination therapy for tuberculosis with next generation benzothiazinones

Pyridomycin bridges the NADH- and substratebinding pockets of the enoyl reductase InhA

Peptide ligands stabilized by small molecules

Structure and Function of Essential Components of Contractile Injection Systems

Phenotypic Profiling of Mycobacterium tuberculosis EspA Point Mutants Reveals that Blockage of ESAT-6 and CFP-10 Secretion In Vitro Does Not Always Correlate with Attenuation of Virulence

Mycobacterium tuberculosis EspB binds phospholipids and mediates EsxA-independent virulence

Tetrahydrobiopterin Biosynthesis as an Off-Target of Sulfa Drugs

Structural Basis for Benzothiazinone-Mediated Killing of Mycobacterium tuberculosis

Towards a new tuberculosis drug: pyridomycin - nature's isoniazid

Characterization of Molecular Determinants of the Conformational Stability of Macrophage Migration Inhibitory Factor: Leucine 46 Hydrophobic Pocket

Virulence Regulator EspR of Mycobacterium tuberculosis Is a Nucleoid-Associated Protein

Directed evolution of the suicide protein O⁶-alkylguanine-DNA alkyltransferase for increased reactivity results in an alkylated protein with exceptional stability

Structure and function of the transketolase from Mycobacterium tuberculosis and comparison with the human enzyme

Benzothiazinones Are Suicide Inhibitors of Mycobacterial Decaprenylphosphoryl-β-

EspD Is Critical for the Virulence-Mediating ESX-1 Secretion System in Mycobacterium tuberculosis

Atypical DNA recognition mechanism used by the EspR virulence regulator of Mycobacterium tuberculosis

Towards anti-virulence drugs targeting ESX-1 mediated pathogenesis of Mycobacterium tuberculosis

Sigma Factor F does not Prevent Rifampin Inhibition of RNA Polymerase or Cause Rifampin Tolerance in Mycobacterium tuberculosis

A finite element model of the LHC dipole cold mass with hysteretic, non-linear behavior and single turn description

Enseignement & Phd

Cours

Integrative structural biology for Life sciences

Recombinant protein expression in animal cells for applications in medicine and structural biology

Methods: from disease models to therapy

Methods: omics in biomedical research

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