Ruud Hovius

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Maître d'enseignement et de recherche

ruud.hovius@epfl.ch +41 21 693 31 34 http://people.epfl.ch/ruud.hovius

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ORCID

0000-0001-9258-6587

EPFL SB ISIC LCBM
CH B3 424 (Bâtiment CH)
Station 6
1015 Lausanne

+41 21 693 31 34
Local: CH B3 424
EPFL > SB > ISIC > LCBM

Web site: Site web: https://lcbm.epfl.ch/

EPFL > SB > SB-SCGC > SCGC-ENS

Web site: Site web: https://www.epfl.ch/schools/sb/scgc/

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Domaines de compétences

Protein structure, expression & purification

Molecular interaction & receptor activation

Fluorescence imaging & spectroscopy

Signalling & sensing

Mission

=> Teaching: Lecture courses, tutorials and practicals in biochemistry, biophysical chemistry of membranes and signalling processes. Participating to the Commission d'Enseignement and Commission de l'Ecole Doctorale of Chemistry and Chemical Engineering.

=> Research interests: Protein function and structure, Membrane protein (especially receptors and channels) expression, structure and function, biomolecular interaction, labeling with biophysical probes, fluorescence imaging and assay and sensor techniques, single molecule techniques.

BSc course EPFL : TP Biochem_Biophys

Practical introducing 6th semester Chemistry Bachelor students in genetic engineering, protein expression and purification, and biochemical and biophysical charaterisation of proteins.

BSc course Molecular and cellular biophysics II

MSc course EPFL : Cellular Signalling

Masters course on advanced concepts of various cellular signalling pathways and their interactions

MSc Course EPFL Chemical Biology

Graduate course : ESONN - Grenoble

Module "Advanced biophysics for microsystems design" à European School on Nanosciences & Nanotechnologies (ESONN.fr) à Grenoble, organisé par IPNG, UJF, CNRS et CEA.

Publications

Publications Infoscience

Infoscience

The HP1 box of KAP1 organizes HP1α for silencing of endogenous retroviral elements in embryonic stem cells

N. Gaurav; R. O’Hara; U. Hyder; W. Qin; C. Her et al.

Nature Communications. 2025. DOI : 10.1038/s41467-025-60279-2.

Ruud Hovius

Maître d'enseignement et de recherche

ORCID

Domaines de compétences

Mission

BSc course EPFL : TP Biochem_Biophys

BSc course Molecular and cellular biophysics II

MSc course EPFL : Cellular Signalling

MSc Course EPFL Chemical Biology

Graduate course : ESONN - Grenoble

Publications

Publications Infoscience

Infoscience

The HP1 box of KAP1 organizes HP1α for silencing of endogenous retroviral elements in embryonic stem cells

A covalently linked probe to monitor local membrane properties surrounding plasma membrane proteins

International Union of Basic and Clinical Pharmacology. CX. Classification of Receptors for 5-hydroxytryptamine; Pharmacology and Function

Torsin ATPases influence chromatin interaction of the Torsin regulator LAP1

Structural mechanism of cGAS inhibition by the nucleosome

A Chemogenetic Approach for the Optical Monitoring of Voltage in Neurons

Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins: Case Study of the Mouse 5-HT3 Receptor

Evaluating Cellular Drug Uptake with Fluorescent Sensor Proteins

Tetrahydrobiopterin Biosynthesis as a Potential Target of the Kynurenine Pathway Metabolite Xanthurenic Acid

Reduction of Neuropathic and Inflammatory Pain through Inhibition of the Tetrahydrobiopterin Pathway

Microfluidic Single-Cell Analysis with Affinity Beads

Insertion of Nanoparticle Clusters into Vesicle Bilayers

A fluorogenic probe for SNAP-tagged plasma membrane proteins based on the solvatochromic molecule Nile Red

Sensory characterization of perfumery raw materials and perfumes: individual sensitivity, trigeminal components and attentional effect

Dithiol amino acids can structurally shape and enhance the ligand-binding properties of polypeptides

Natural compounds boldine and menthol are antagonists of human 5-HT

X-ray structure of the mouse serotonin 5-HT3 receptor

Characterization and Validation of Fluorescent Receptor Ligands: A Case Study of the Ionotropic Serotonin Receptor

Downscaling the Analysis of Complex Transmembrane Signaling Cascades to Closed Attoliter Volumes

Microfluidic single cell analysis with affinity beads

Cell-derived plasma membrane vesicles as minimal cells for analyzing transmembrane signaling

Correlated Optical and Electrical Single-Molecule Measurements Reveal Conformational Diffusion from Ligand Binding to Channel Gating in the Nicotinic Acetylcholine Receptor

Acetylcholine Receptor Organization in Membrane Domains in Muscle Cells: EVIDENCE FOR RAPSYN-INDEPENDENT AND RAPSYN-DEPENDENT MECHANISMS

Activation of G-Protein-Coupled Receptors in Cell-Derived Plasma Membranes Supported on Porous Beads

Advanced Fluorescence Microscopy to Study Plasma Membrane Protein Dynamics

Serotonin receptor diversity in the human colon: Expression of serotonin type 3 receptor subunits 5-HT3C, 5-HT3D, and 5-HT3E

New conjugate of agonist ligand directed to ligand-gated ion channels of cysteine loop-family with chromophore, useful, e.g. in receptor proteins/their interaction with agonistic ligand and to study the effect of unlabelled ligands

Fluorescent Epibatidine Agonists for Neuronal and Muscle-Type Nicotinic Acetylcholine Receptors

Repetitive Reversible Labeling of Proteins at Polyhistidine Sequences for Single-Molecule Imaging in Live Cells

Modulation of proton-induced current fluctuations in the human nicotinic acetylcholine receptor channel

Fluorescent Labelling of Membrane Proteins in Living Cells

FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells

Determining a receptor-ligand interaction useful in e.g. drug screening involves repetitive application of reversibly binding fluorescent ligands with fast association-dissociation kinetics

Functional asymmetry of transmembrane segments in nicotinic acetylcholine receptors

Covalent labeling of cell-surface proteins for in-vivo FRET studies

Synthesis of nanoscopic optical fibers using lipid membranes as templates

Lipid-coated nanocrystals as multifunctionalized luminescent scaffolds for supramolecular biological assemblies

Investigating cellular signaling reactions in single attoliter vesicles

Reversible sequential-binding probe receptor-ligand interactions in single cells

Reversible site-selective labeling of membrane proteins in live cells

Highly Fluorescent Streptavidin-Coated CdSe Nanoparticles: Preparation in Water, Characterization, and Micropatterning

Ligand Binding to G Protein-Coupled Receptors in Tethered Cell Membranes

Characterization of the Ligand-binding Site of the Serotonin 5-HT3 Receptor: The Role of Glutamate Residues 97, 224,and 235

Downscaling Fourier Transform Infrared Spectroscopy to the Micrometer and Nanogram Scale: Secondary Structure of Serotonin and Acetylcholine Receptors

Monitoring expression and clustering of the ionotropic 5HT3 receptor in plasma membranes of live biological cells

Factors influencing fluorescence correlation spectroscopy measurements on membranes: simulations and experiments

Characterization of a transmembrane receptor protein by fluorescence correlation spectroscopy

Mapping the Antagonist Binding Site of the Serotonin Type 3 Receptor by Fluorescence Resonance Energy Transfer

In vitro and in vivo ligand binding to the 5HT3 serotonin receptor characterised by time-resolved fluorescence spectroscopy

Functional immobilisation of the nicotinic acetylcholine receptor in tethered lipid membranes

Transient-expression technologies, their application and scale-up: 5-HT3 serotonin receptor case study

Fluorescence techniques: shedding light on ligand-receptor interactions

Large scale transient 5-HT3 receptor production with the Semliki Forest Virus Expression System

Study of ligand-receptor interactions by fluorescence correlation spectroscopy with different fluorophores: Evidence that the homopentameric 5-hydroxytryptamine type 3(As) receptor binds only one ligand

Fluorescence techniques for fundamental and applied studies of membrane protein receptors: the 5-HT3 serotonin receptor

Waveguide fluorosensor for the detection of ligand-receptor interactions - The 5-HT3 serotonin receptor

Characterization of a mouse serotonin 5-HT3 receptor purified from mammalian cells

Ligand binding to the serotonin 5HT3 receptor studied with a novel fluorescent ligand

Screening Ligands for Membrane Protein Receptors by Total Internal Reflectance Fluorescence: The 5-HT3 Serotonin Receptor

The Semliki Forest virus system: receptor expression and scale-up

Expression of ligand-gated ion channels with the Semliki Forest virus expression system

Metal ion trace detection by a chelator-modified gold electrode: A comparison of surface to bulk affinity

Phospholipid asymmetry of the outer membrane of rat liver mitochondria: Evidence for the presence of cardiolipin on the outside of the outer membrane

On the mechanism of the mitochondrial decarboxylation of phosphatidylserine

Interaction of mitochondrial creatine kinase with model membranes: A monolayer study

Mitochondrial creatine kinase mediates contact formation between mitochondrial membranes

The phosphatidylcholine-transfer protein catalyzed import of phosphatidylcholine into isolated rat liver mitochondria